Details for: ZNF214

Gene ID: 7761

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF214

Ensembl ID: ENSG00000149050

Description: zinc finger protein 214

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • multi-ciliated epithelial cell CL0005012
    CSI 3.33
    rCSI 3.32%
    PRS 96.93
  • lung ciliated cell CL1000271
    CSI 3.08
    rCSI 3.56%
    PRS 97.09
  • ciliated epithelial cell CL0000067
    CSI 2.96
    rCSI 2.61%
    PRS 96.01
  • ependymal cell CL0000065
    CSI 2.39
    rCSI 4.85%
    PRS 92.81
  • ciliated cell CL0000064
    CSI 2.12
    rCSI 3.44%
    PRS 96.26

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

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  • Node Color (Target Cell CSI, relative to current network):
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  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF214](/details-gene/7761) is a protein-coding gene located on chromosome 11p15.4 that encodes zinc finger protein 214. Functional annotations strongly indicate that [ZNF214](/details-gene/7761) acts as a nuclear DNA-binding transcription factor involved in the regulation of gene expression. Expression data reveal a highly specific role for this gene, as it is a significant marker for several types of ciliated cells, including those in the respiratory and central nervous systems. Its sequence was characterized as part of large-scale cDNA and genome sequencing projects ([Link](https://doi.org/10.1038/ng1285), [Link](https://doi.org/10.1038/nature04632)). ## Cellular Roles and Expression Landscape **Overall**, the expression profile of [ZNF214](/details-gene/7761) demonstrates remarkable specificity for ciliated cell lineages, suggesting a crucial role in their identity or function. The gene shows the highest significance in [multi-ciliated epithelial cell](/details-cell/CL0005012) (CSI: 3.33), [lung ciliated cell](/details-cell/CL1000271) (CSI: 3.08), and [ciliated epithelial cell](/details-cell/CL0000067) (CSI: 2.96). Its strong association extends to other specialized ciliated populations, such as the [ependymal cell](/details-cell/CL0000065) (CSI: 2.39) lining the brain ventricles and the broader category of [ciliated cell](/details-cell/CL0000064) (CSI: 2.12). This consistent and high-level expression across multiple tissues where ciliated cells are present points to [ZNF214](/details-gene/7761) as a core transcriptional regulator for this specialized cell type. A related zinc-finger gene, ZNF215, has been implicated in the developmental disorder Beckwith-Wiedemann syndrome, suggesting that genes in this family may have important roles in differentiation and development ([Link](https://doi.org/10.1086/302892)). ## Pathways and Molecular Function Functionally, [ZNF214](/details-gene/7761) is firmly placed within the machinery of transcriptional control. As a nuclear protein ([GO:0005634](https://www.ebi.ac.uk/QuickGO/term/GO:0005634)), its molecular activities include [Dna binding](/details-go/GO:0003677), [Dna-binding transcription factor activity](/details-go/GO:0003700), and [Zinc ion binding](/details-go/GO:0008270), the latter being characteristic of its zinc finger protein structure. These functions are integral to the biological process of [Regulation of dna-templated transcription](/details-go/GO:0006355). This role is further supported by its annotation in Reactome pathways such as [Gene expression (transcription)](https://reactome.org/content/detail/R-HSA-74160) and [Rna polymerase ii transcription](https://reactome.org/content/detail/R-HSA-73857). This suggests that the high expression of [ZNF214](/details-gene/7761) in ciliated cells is likely to direct a specific gene expression program required for their differentiation, maturation, or the maintenance of their complex motile machinery. ## Research Directions The highly specific expression of [ZNF214](/details-gene/7761) in ciliated cells, coupled with its function as a transcription factor, presents clear avenues for future research. **Proposed Hypotheses:** 1. [ZNF214](/details-gene/7761) is a master regulator of ciliogenesis, controlling the expression of a network of genes essential for the formation and structural integrity of cilia. Loss-of-function mutations in [ZNF214](/details-gene/7761) may therefore be a cause of primary ciliary dyskinesia (PCD) or other ciliopathies. 2. The primary role of [ZNF214](/details-gene/7761) is not in the initial formation of cilia but in the maintenance of their function in terminally differentiated cells, such as regulating the expression of genes involved in ciliary motility or intraflagellar transport in response to environmental stimuli. **Suggested Experimental Approach:** To test the hypothesis that [ZNF214](/details-gene/7761) is a master regulator of ciliogenesis, an *in vitro* model using primary human bronchial epithelial cells (HBECs) grown at an air-liquid interface (ALI) would be ideal. CRISPR-Cas9 could be used to generate a [ZNF214](/details-gene/7761) knockout in these cells prior to inducing differentiation. The impact on the formation of a ciliated epithelium could be quantified via immunofluorescence staining for ciliary markers (e.g., acetylated tubulin) and transmission electron microscopy to assess cilia ultrastructure. A comparative RNA-seq analysis between wild-type and knockout cells during the differentiation time-course would identify the downstream transcriptional targets of [ZNF214](/details-gene/7761) and reveal the specific pathways it regulates. **Therapeutic Potential:** Given its highly restricted expression pattern, [ZNF214](/details-gene/7761) is an unlikely target for broad therapeutic intervention in common diseases like cancer or inflammation. Its primary clinical relevance may lie in rare genetic disorders. If mutations in [ZNF214](/details-gene/7761) are identified as a monogenic cause of a ciliopathy, it could become a candidate for gene replacement therapies. Such a strategy would aim to restore, rather than inhibit, its function in affected tissues like the respiratory epithelium.

Genular Protein ID: 2167626383

Symbol: ZN214_HUMAN

Name: Zinc finger protein 214

UniProtKB Accession Codes:

Q9UL59 B2R8Q1

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 4 Ensembl 2 GO 4 Gene3D 2 GeneCards 1 GeneTree 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 4 KEGG 1 MANE-Select 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 Reactome 1 RefSeq 3 SMART 2 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 10762538

Title: Disruption of a novel imprinted zinc-finger gene, ZNF215, in Beckwith-Wiedemann syndrome.

PubMed ID: 10762538

DOI: 10.1086/302892

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 16554811

Title: Human chromosome 11 DNA sequence and analysis including novel gene identification.

PubMed ID: 16554811

DOI: 10.1038/nature04632

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 606
  • Mass: 70992
  • Checksum: D0CBA2E0F9E3EAB2
  • Sequence:
    • MAVTFEDVTI IFTWEEWKFL DSSQKRLYRE VMWENYTNVM SVENWNESYK SQEEKFRYLE 
YENFSYWQGW WNAGAQMYEN QNYGETVQGT DSKDLTQQDR SQCQEWLILS TQVPGYGNYE 
LTFESKSLRN LKYKNFMPWQ SLETKTTQDY GREIYMSGSH GFQGGRYRLG ISRKNLSMEK 
EQKLIVQHSY IPVEEALPQY VGVICQEDLL RDSMEEKYCG CNKCKGIYYW NSRCVFHKRN 
QPGENLCQCS ICKACFSQRS DLYRHPRNHI GKKLYGCDEV DGNFHQSSGV HFHQRVHIGE 
VPYSCNACGK SFSQISSLHN HQRVHTEEKF YKIECDKDLS RNSLLHIHQR LHIGEKPFKC 
NQCGKSFNRS SVLHVHQRVH TGEKPYKCDE CGKGFSQSSN LRIHQLVHTG EKSYKCEDCG 
KGFTQRSNLQ IHQRVHTGEK PYKCDDCGKD FSHSSDLRIH QRVHTGEKPY TCPECGKGFS 
KSSKLHTHQR VHTGEKPYKC EECGKGFSQR SHLLIHQRVH TGEKPYKCHD CGKGFSHSSN 
LHIHQRVHTG EKPYQCAKCG KGFSHSSALR IHQRVHAGEK PYKCREYYKG FDHNSHLHNN 
HRRGNL