Details for: HUS1

Gene ID: 3364

Symbol: HUS1

Ensembl ID: ENSG00000136273

Description: HUS1 checkpoint clamp component

Associated with

Cells (max top 100)

(Marker Score score is uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: kidney capillary endothelial cell (CL1000892)
    Fold Change: 1.39
    Marker Score: 436
  • Cell Name: kidney proximal straight tubule epithelial cell (CL1000839)
    Fold Change: 1.28
    Marker Score: 3024
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: 1.25
    Marker Score: 19503
  • Cell Name: immature innate lymphoid cell (CL0001082)
    Fold Change: 1.17
    Marker Score: 2383.5
  • Cell Name: primordial germ cell (CL0000670)
    Fold Change: 1.17
    Marker Score: 1466
  • Cell Name: mononuclear cell (CL0000842)
    Fold Change: 1.15
    Marker Score: 372
  • Cell Name: pulmonary interstitial fibroblast (CL0002241)
    Fold Change: 1.02
    Marker Score: 812.5
  • Cell Name: cerebral cortex GABAergic interneuron (CL0010011)
    Fold Change: 1
    Marker Score: 71705
  • Cell Name: forebrain radial glial cell (CL0013000)
    Fold Change: 1
    Marker Score: 47929
  • Cell Name: absorptive cell (CL0000212)
    Fold Change: 0.98
    Marker Score: 30394
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 0.94
    Marker Score: 2398
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: 0.93
    Marker Score: 1968
  • Cell Name: lymphoid lineage restricted progenitor cell (CL0000838)
    Fold Change: 0.93
    Marker Score: 559
  • Cell Name: fibroblast of cardiac tissue (CL0002548)
    Fold Change: 0.93
    Marker Score: 5574
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 0.93
    Marker Score: 478
  • Cell Name: BEST4+ intestinal epithelial cell, human (CL4030026)
    Fold Change: 0.92
    Marker Score: 434
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: 0.9
    Marker Score: 2705
  • Cell Name: proerythroblast (CL0000547)
    Fold Change: 0.88
    Marker Score: 595
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.87
    Marker Score: 5288
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.86
    Marker Score: 308
  • Cell Name: transit amplifying cell (CL0009010)
    Fold Change: 0.85
    Marker Score: 4840
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: 0.83
    Marker Score: 8571
  • Cell Name: connective tissue cell (CL0002320)
    Fold Change: 0.81
    Marker Score: 210
  • Cell Name: elicited macrophage (CL0000861)
    Fold Change: 0.8
    Marker Score: 4841
  • Cell Name: intestinal epithelial cell (CL0002563)
    Fold Change: 0.78
    Marker Score: 1265
  • Cell Name: early T lineage precursor (CL0002425)
    Fold Change: 0.77
    Marker Score: 591
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.77
    Marker Score: 307
  • Cell Name: smooth muscle myoblast (CL0000514)
    Fold Change: 0.73
    Marker Score: 349.5
  • Cell Name: endothelial cell of vascular tree (CL0002139)
    Fold Change: 0.73
    Marker Score: 1063
  • Cell Name: Cajal-Retzius cell (CL0000695)
    Fold Change: 0.72
    Marker Score: 371
  • Cell Name: blood cell (CL0000081)
    Fold Change: 0.71
    Marker Score: 8208
  • Cell Name: large pre-B-II cell (CL0000957)
    Fold Change: 0.7
    Marker Score: 1915.5
  • Cell Name: alveolar macrophage (CL0000583)
    Fold Change: 0.7
    Marker Score: 17930
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.69
    Marker Score: 2903
  • Cell Name: Bergmann glial cell (CL0000644)
    Fold Change: 0.68
    Marker Score: 279
  • Cell Name: choroid plexus epithelial cell (CL0000706)
    Fold Change: 0.67
    Marker Score: 618
  • Cell Name: neutrophil progenitor cell (CL0000834)
    Fold Change: 0.67
    Marker Score: 172
  • Cell Name: brush cell (CL0002204)
    Fold Change: 0.67
    Marker Score: 612
  • Cell Name: primitive red blood cell (CL0002355)
    Fold Change: 0.67
    Marker Score: 500
  • Cell Name: ependymal cell (CL0000065)
    Fold Change: 0.67
    Marker Score: 232
  • Cell Name: plasmablast (CL0000980)
    Fold Change: 0.65
    Marker Score: 870
  • Cell Name: epithelial cell of esophagus (CL0002252)
    Fold Change: 0.65
    Marker Score: 5559
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: 0.64
    Marker Score: 943
  • Cell Name: preosteoblast (CL0007010)
    Fold Change: 0.64
    Marker Score: 181
  • Cell Name: L6b glutamatergic cortical neuron (CL4023038)
    Fold Change: 0.64
    Marker Score: 5465
  • Cell Name: hepatocyte (CL0000182)
    Fold Change: 0.63
    Marker Score: 430
  • Cell Name: CD14-positive, CD16-positive monocyte (CL0002397)
    Fold Change: 0.63
    Marker Score: 1224
  • Cell Name: gut absorptive cell (CL0000677)
    Fold Change: 0.63
    Marker Score: 402
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 0.63
    Marker Score: 38532
  • Cell Name: precursor B cell (CL0000817)
    Fold Change: 0.63
    Marker Score: 414
  • Cell Name: extravillous trophoblast (CL0008036)
    Fold Change: 0.62
    Marker Score: 555
  • Cell Name: corticothalamic-projecting glutamatergic cortical neuron (CL4023013)
    Fold Change: 0.61
    Marker Score: 5828
  • Cell Name: L5 extratelencephalic projecting glutamatergic cortical neuron (CL4023041)
    Fold Change: 0.61
    Marker Score: 939
  • Cell Name: mature B cell (CL0000785)
    Fold Change: 0.6
    Marker Score: 439
  • Cell Name: mature alpha-beta T cell (CL0000791)
    Fold Change: 0.59
    Marker Score: 31347
  • Cell Name: colon epithelial cell (CL0011108)
    Fold Change: 0.59
    Marker Score: 1854
  • Cell Name: CD4-positive helper T cell (CL0000492)
    Fold Change: 0.59
    Marker Score: 641
  • Cell Name: neuronal receptor cell (CL0000006)
    Fold Change: 0.59
    Marker Score: 260
  • Cell Name: CD8-positive, alpha-beta memory T cell, CD45RO-positive (CL0001203)
    Fold Change: 0.59
    Marker Score: 1642
  • Cell Name: endothelial cell of hepatic sinusoid (CL1000398)
    Fold Change: 0.58
    Marker Score: 128
  • Cell Name: epicardial adipocyte (CL1000309)
    Fold Change: 0.58
    Marker Score: 282
  • Cell Name: erythroid progenitor cell (CL0000038)
    Fold Change: 0.58
    Marker Score: 602
  • Cell Name: double negative thymocyte (CL0002489)
    Fold Change: 0.58
    Marker Score: 795
  • Cell Name: osteoblast (CL0000062)
    Fold Change: 0.58
    Marker Score: 309
  • Cell Name: CD8-positive, alpha-beta cytokine secreting effector T cell (CL0000908)
    Fold Change: 0.57
    Marker Score: 247
  • Cell Name: decidual cell (CL2000002)
    Fold Change: 0.57
    Marker Score: 2461
  • Cell Name: effector CD4-positive, alpha-beta T cell (CL0001044)
    Fold Change: 0.57
    Marker Score: 533
  • Cell Name: Unknown (CL0000548)
    Fold Change: 0.57
    Marker Score: 415
  • Cell Name: IgG memory B cell (CL0000979)
    Fold Change: 0.57
    Marker Score: 342
  • Cell Name: IgG-negative class switched memory B cell (CL0002117)
    Fold Change: 0.57
    Marker Score: 558
  • Cell Name: CD14-positive, CD16-negative classical monocyte (CL0002057)
    Fold Change: 0.57
    Marker Score: 31662
  • Cell Name: fraction A pre-pro B cell (CL0002045)
    Fold Change: 0.57
    Marker Score: 569
  • Cell Name: podocyte (CL0000653)
    Fold Change: 0.56
    Marker Score: 208
  • Cell Name: kidney epithelial cell (CL0002518)
    Fold Change: 0.56
    Marker Score: 594
  • Cell Name: effector CD8-positive, alpha-beta T cell (CL0001050)
    Fold Change: 0.56
    Marker Score: 472
  • Cell Name: lung microvascular endothelial cell (CL2000016)
    Fold Change: 0.56
    Marker Score: 119
  • Cell Name: myeloid cell (CL0000763)
    Fold Change: 0.56
    Marker Score: 895
  • Cell Name: mature gamma-delta T cell (CL0000800)
    Fold Change: 0.56
    Marker Score: 1755
  • Cell Name: megakaryocyte-erythroid progenitor cell (CL0000050)
    Fold Change: 0.56
    Marker Score: 232
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: 0.55
    Marker Score: 254
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 0.55
    Marker Score: 131
  • Cell Name: intestinal crypt stem cell of large intestine (CL0009016)
    Fold Change: 0.54
    Marker Score: 163
  • Cell Name: neuron (CL0000540)
    Fold Change: 0.54
    Marker Score: 2206
  • Cell Name: erythroblast (CL0000765)
    Fold Change: 0.54
    Marker Score: 337
  • Cell Name: pancreatic stellate cell (CL0002410)
    Fold Change: 0.54
    Marker Score: 340
  • Cell Name: osteoclast (CL0000092)
    Fold Change: 0.54
    Marker Score: 267
  • Cell Name: intestinal enteroendocrine cell (CL1001516)
    Fold Change: 0.54
    Marker Score: 427
  • Cell Name: mucosal invariant T cell (CL0000940)
    Fold Change: 0.53
    Marker Score: 475
  • Cell Name: mononuclear phagocyte (CL0000113)
    Fold Change: 0.53
    Marker Score: 536
  • Cell Name: kidney loop of Henle thin descending limb epithelial cell (CL1001111)
    Fold Change: 0.53
    Marker Score: 575
  • Cell Name: mesenchymal lymphangioblast (CL0005021)
    Fold Change: 0.53
    Marker Score: 119
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: 0.53
    Marker Score: 2089
  • Cell Name: double-positive, alpha-beta thymocyte (CL0000809)
    Fold Change: 0.53
    Marker Score: 1040
  • Cell Name: megakaryocyte (CL0000556)
    Fold Change: 0.53
    Marker Score: 300
  • Cell Name: near-projecting glutamatergic cortical neuron (CL4023012)
    Fold Change: 0.53
    Marker Score: 4944.5
  • Cell Name: cardiac endothelial cell (CL0010008)
    Fold Change: 0.52
    Marker Score: 994
  • Cell Name: glomerular endothelial cell (CL0002188)
    Fold Change: 0.52
    Marker Score: 133
  • Cell Name: melanocyte (CL0000148)
    Fold Change: 0.52
    Marker Score: 212
  • Cell Name: tendon cell (CL0000388)
    Fold Change: 0.52
    Marker Score: 128
  • Cell Name: neural cell (CL0002319)
    Fold Change: 0.52
    Marker Score: 251

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Other Information

**Key characteristics:** * The HUS1 protein is a component of the checkpoint clamp, a regulatory mechanism that prevents DNA damage from triggering the DNA repair pathway. * The protein is expressed in a variety of cell types, including kidney capillary endothelial cells, cardiac muscle myoblasts, and immune cells. * The protein is involved in the regulation of the cell cycle and DNA repair pathways through its interaction with the ATR (ATR) protein. * Mutations in the HUS1 gene have been linked to a number of human diseases, including cancer and cardiovascular disease. **Pathways and functions:** * The HUS1 checkpoint clamp component is activated in response to DNA damage or replication stress. * Upon activation, the HUS1 protein recruits the ATR protein to the site of DNA damage. * The ATR protein then activates the p53 protein, which inhibits the cell cycle progression. * The HUS1 checkpoint clamp component also regulates the expression of genes involved in DNA repair, including those involved in DNA damage detection, repair, and cell cycle checkpoints. **Clinical significance:** Mutations in the HUS1 gene have been linked to a number of human diseases, including: * Cancer: Mutations in the HUS1 gene have been found in a significant proportion of human cancers, including lung cancer, breast cancer, and colon cancer. * Cardiovascular disease: Mutations in the HUS1 gene have been linked to an increased risk of cardiovascular disease, including heart attacks and strokes. * Other diseases: Mutations in the HUS1 gene have also been linked to other diseases, such as autoimmune disorders, neurodegenerative diseases, and cancer. **Overall, the HUS1 checkpoint clamp component is a critical regulator of the cell cycle and DNA repair pathways in humans. Mutations in this gene have significant clinical implications, as they can lead to the development of a variety of human diseases.**

Genular Protein ID: 236535009

Symbol: HUS1_HUMAN

Name: Checkpoint protein HUS1

UniProtKB Accession Codes:

O60921 B4DFI9

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 BRENDA 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 2 CORUM 1 CPTC 1 CTD 1 ChiTaRS 1 ComplexPortal 1 DIP 1 DNASU 1 DisGeNET 1 EMBL 12 EMDB 1 EPD 1 Ensembl 3 EvolutionaryTrace 1 ExpressionAtlas 1 GO 19 Gene3D 1 GeneCards 1 GeneTree 1 GeneWiki 1 Genevisible 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 3 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 MaxQB 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 12 PDBsum 6 PIRSF 1 PRO 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 2 Pumba 1 RNAct 1 Reactome 7 RefSeq 2 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TopDownProteomics 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 9524127

Title: Hus1p, a conserved fission yeast checkpoint protein, interacts with Rad1p and is phosphorylated in response to DNA damage.

PubMed ID: 9524127

DOI: 10.1093/emboj/17.7.2055

PubMed ID: 9878245

Title: cDNA cloning and gene mapping of human homologs for Schizosaccharomyces pombe rad17, rad1, and hus1 and cloning of homologs from mouse, Caenorhabditis elegans, and Drosophila melanogaster.

PubMed ID: 9878245

DOI: 10.1006/geno.1998.5587

PubMed ID: 10777662

Title: Physical interaction among human checkpoint control proteins HUS1p, RAD1p, and RAD9p, and implications for the regulation of cell cycle progression.

PubMed ID: 10777662

DOI: 10.1006/geno.2000.6142

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 12853948

Title: The DNA sequence of human chromosome 7.

PubMed ID: 12853948

DOI: 10.1038/nature01782

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 10359610

Title: The human G2 checkpoint control protein hRAD9 is a nuclear phosphoprotein that forms complexes with hRAD1 and hHUS1.

PubMed ID: 10359610

DOI: 10.1091/mbc.10.6.1985

PubMed ID: 10846170

Title: HDAC1, a histone deacetylase, forms a complex with Hus1 and Rad9, two G2/M checkpoint Rad proteins.

PubMed ID: 10846170

DOI: 10.1074/jbc.m000168200

PubMed ID: 10884395

Title: The human checkpoint protein hRad17 interacts with the PCNA-like proteins hRad1, hHus1, and hRad9.

PubMed ID: 10884395

DOI: 10.1074/jbc.m005782200

PubMed ID: 11077446

Title: PCNA interacts with hHus1/hRad9 in response to DNA damage and replication inhibition.

PubMed ID: 11077446

DOI: 10.1038/sj.onc.1203901

PubMed ID: 11573955

Title: The J domain of Tpr2 regulates its interaction with the proapoptotic and cell-cycle checkpoint protein, Rad9.

PubMed ID: 11573955

DOI: 10.1006/bbrc.2001.5685

PubMed ID: 14500360

Title: Expression of mammalian paralogues of HRAD9 and Mrad9 checkpoint control genes in normal and cancerous testicular tissue.

PubMed ID: 14500360

PubMed ID: 14611806

Title: Identification and characterization of RAD9B, a paralog of the RAD9 checkpoint gene.

PubMed ID: 14611806

DOI: 10.1016/s0888-7543(03)00200-3

PubMed ID: 12578958

Title: Loading of the human 9-1-1 checkpoint complex onto DNA by the checkpoint clamp loader hRad17-replication factor C complex in vitro.

PubMed ID: 12578958

DOI: 10.1073/pnas.0437927100

PubMed ID: 15314187

Title: The human Rad9/Rad1/Hus1 damage sensor clamp interacts with DNA polymerase beta and increases its DNA substrate utilisation efficiency: implications for DNA repair.

PubMed ID: 15314187

DOI: 10.1093/nar/gkh652

PubMed ID: 15556996

Title: The human Rad9-Rad1-Hus1 checkpoint complex stimulates flap endonuclease 1.

PubMed ID: 15556996

DOI: 10.1073/pnas.0407686101

PubMed ID: 15871698

Title: The human checkpoint sensor and alternative DNA clamp Rad9-Rad1-Hus1 modulates the activity of DNA ligase I, a component of the long-patch base excision repair machinery.

PubMed ID: 15871698

DOI: 10.1042/bj20050211

PubMed ID: 16216273

Title: The two DNA clamps Rad9/Rad1/Hus1 complex and proliferating cell nuclear antigen differentially regulate flap endonuclease 1 activity.

PubMed ID: 16216273

DOI: 10.1016/j.jmb.2005.09.018

PubMed ID: 15897895

Title: Interaction and colocalization of Rad9/Rad1/Hus1 checkpoint complex with replication protein A in human cells.

PubMed ID: 15897895

DOI: 10.1038/sj.onc.1208674

PubMed ID: 21659603

Title: A DNA damage response screen identifies RHINO, a 9-1-1 and TopBP1 interacting protein required for ATR signaling.

PubMed ID: 21659603

DOI: 10.1126/science.1203430

PubMed ID: 31776186

Title: Structure of the RAD9-RAD1-HUS1 checkpoint clamp bound to RHINO sheds light on the other side of the DNA clamp.

PubMed ID: 31776186

DOI: 10.1074/jbc.ac119.011816

PubMed ID: 36841485

Title: The 9-1-1 DNA clamp subunit RAD1 forms specific interactions with clamp loader RAD17, revealing functional implications for binding-protein RHINO.

PubMed ID: 36841485

DOI: 10.1016/j.jbc.2023.103061

Sequence Information:

  • Length: 280
  • Mass: 31691
  • Checksum: 9C3ED2FD35C2ACB3
  • Sequence:
    • MKFRAKIVDG ACLNHFTRIS NMIAKLAKTC TLRISPDKLN FILCDKLANG GVSMWCELEQ 
ENFFNEFQME GVSAENNEIY LELTSENLSR ALKTAQNARA LKIKLTNKHF PCLTVSVELL 
SMSSSSRIVT HDIPIKVIPR KLWKDLQEPV VPDPDVSIYL PVLKTMKSVV EKMKNISNHL 
VIEANLDGEL NLKIETELVC VTTHFKDLGN PPLASESTHE DRNVEHMAEV HIDIRKLLQF 
LAGQQVNPTK ALCNIVNNKM VHFDLLHEDV SLQYFIPALS

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. For the full schema, download it here.