Details for: NCF4

Gene ID: 4689

Symbol: NCF4

Ensembl ID: ENSG00000100365

Description: neutrophil cytosolic factor 4

Associated with

Cells (max top 100)

(Cell Significance Index and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: polychromatophilic erythroblast (CL0000550)
    Fold Change: 57.9896
    Cell Significance Index: -9.0200
  • Cell Name: mucosal type mast cell (CL0000485)
    Fold Change: 27.8641
    Cell Significance Index: -11.3200
  • Cell Name: peripheral blood mononuclear cell (CL2000001)
    Fold Change: 23.9509
    Cell Significance Index: -12.3200
  • Cell Name: ciliated cell of the bronchus (CL0002332)
    Fold Change: 12.1918
    Cell Significance Index: -11.6400
  • Cell Name: orthochromatic erythroblast (CL0000552)
    Fold Change: 7.4617
    Cell Significance Index: -9.2000
  • Cell Name: centroblast (CL0009112)
    Fold Change: 6.8108
    Cell Significance Index: 15.8900
  • Cell Name: pro-T cell (CL0000827)
    Fold Change: 3.2247
    Cell Significance Index: 82.3800
  • Cell Name: epidermal Langerhans cell (CL0002457)
    Fold Change: 3.1070
    Cell Significance Index: -6.8000
  • Cell Name: CD14-positive, CD16-negative classical monocyte (CL0002057)
    Fold Change: 2.9292
    Cell Significance Index: 54.1400
  • Cell Name: CD8-positive, alpha-beta regulatory T cell (CL0000795)
    Fold Change: 2.8521
    Cell Significance Index: -8.7600
  • Cell Name: stromal cell of bone marrow (CL0010001)
    Fold Change: 2.4810
    Cell Significance Index: -9.7900
  • Cell Name: CD8-alpha-beta-positive, alpha-beta intraepithelial T cell (CL0000796)
    Fold Change: 2.2435
    Cell Significance Index: -6.0100
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: 2.2319
    Cell Significance Index: 143.9900
  • Cell Name: CD4-positive, alpha-beta memory T cell, CD45RO-positive (CL0001204)
    Fold Change: 1.8921
    Cell Significance Index: 55.5700
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 1.5027
    Cell Significance Index: 177.2200
  • Cell Name: enteroendocrine cell of colon (CL0009042)
    Fold Change: 1.3651
    Cell Significance Index: 259.7900
  • Cell Name: hematopoietic oligopotent progenitor cell (CL0002032)
    Fold Change: 0.9856
    Cell Significance Index: -0.2500
  • Cell Name: colon goblet cell (CL0009039)
    Fold Change: 0.9409
    Cell Significance Index: 93.0800
  • Cell Name: neutrophil progenitor cell (CL0000834)
    Fold Change: 0.8788
    Cell Significance Index: 23.5100
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 0.7608
    Cell Significance Index: 686.9400
  • Cell Name: intestinal crypt stem cell of small intestine (CL0009017)
    Fold Change: 0.7602
    Cell Significance Index: 16.1900
  • Cell Name: late promyelocyte (CL0002151)
    Fold Change: 0.7235
    Cell Significance Index: 4.8000
  • Cell Name: myeloid leukocyte (CL0000766)
    Fold Change: 0.6444
    Cell Significance Index: 5.2200
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.4858
    Cell Significance Index: 79.0200
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 0.4749
    Cell Significance Index: 51.6500
  • Cell Name: gut absorptive cell (CL0000677)
    Fold Change: 0.3536
    Cell Significance Index: 21.2300
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 0.1681
    Cell Significance Index: 11.6300
  • Cell Name: early promyelocyte (CL0002154)
    Fold Change: 0.1175
    Cell Significance Index: 0.7500
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: 0.1111
    Cell Significance Index: 5.7700
  • Cell Name: umbrella cell of urothelium (CL4030056)
    Fold Change: 0.0831
    Cell Significance Index: 0.7700
  • Cell Name: paneth cell of epithelium of small intestine (CL1000343)
    Fold Change: 0.0822
    Cell Significance Index: 1.7800
  • Cell Name: mononuclear cell (CL0000842)
    Fold Change: 0.0661
    Cell Significance Index: 0.2800
  • Cell Name: intermediate cell of urothelium (CL4030055)
    Fold Change: 0.0444
    Cell Significance Index: 8.0100
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.0056
    Cell Significance Index: 0.1600
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: 0.0046
    Cell Significance Index: 0.4700
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: 0.0007
    Cell Significance Index: 0.0200
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: -0.0006
    Cell Significance Index: -0.0200
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: -0.0048
    Cell Significance Index: -9.1100
  • Cell Name: enteroendocrine cell of small intestine (CL0009006)
    Fold Change: -0.0060
    Cell Significance Index: -0.1500
  • Cell Name: lens epithelial cell (CL0002224)
    Fold Change: -0.0060
    Cell Significance Index: -9.2000
  • Cell Name: enterocyte of epithelium of large intestine (CL0002071)
    Fold Change: -0.0078
    Cell Significance Index: -0.3600
  • Cell Name: pulmonary alveolar epithelial cell (CL0000322)
    Fold Change: -0.0082
    Cell Significance Index: -6.2400
  • Cell Name: basal cell of urothelium (CL1000486)
    Fold Change: -0.0088
    Cell Significance Index: -1.0900
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: -0.0132
    Cell Significance Index: -9.7700
  • Cell Name: kidney loop of Henle cortical thick ascending limb epithelial cell (CL1001109)
    Fold Change: -0.0155
    Cell Significance Index: -11.3500
  • Cell Name: pancreatic PP cell (CL0002275)
    Fold Change: -0.0159
    Cell Significance Index: -9.9400
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: -0.0167
    Cell Significance Index: -9.1200
  • Cell Name: hair follicular keratinocyte (CL2000092)
    Fold Change: -0.0171
    Cell Significance Index: -7.5600
  • Cell Name: non-pigmented ciliary epithelial cell (CL0002304)
    Fold Change: -0.0172
    Cell Significance Index: -10.9100
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: -0.0173
    Cell Significance Index: -9.7800
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: -0.0227
    Cell Significance Index: -10.2900
  • Cell Name: hematopoietic cell (CL0000988)
    Fold Change: -0.0232
    Cell Significance Index: -0.3400
  • Cell Name: obsolete caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: -0.0274
    Cell Significance Index: -9.8100
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: -0.0288
    Cell Significance Index: -8.2800
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: -0.0396
    Cell Significance Index: -7.8500
  • Cell Name: pancreatic D cell (CL0000173)
    Fold Change: -0.0453
    Cell Significance Index: -9.5400
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: -0.0477
    Cell Significance Index: -9.5600
  • Cell Name: fibro/adipogenic progenitor cell (CL0009099)
    Fold Change: -0.0499
    Cell Significance Index: -2.5200
  • Cell Name: paneth cell of colon (CL0009009)
    Fold Change: -0.0527
    Cell Significance Index: -0.7900
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: -0.0550
    Cell Significance Index: -9.3900
  • Cell Name: transit amplifying cell of small intestine (CL0009012)
    Fold Change: -0.0583
    Cell Significance Index: -1.2100
  • Cell Name: stromal cell of ovary (CL0002132)
    Fold Change: -0.0583
    Cell Significance Index: -8.0100
  • Cell Name: luminal adaptive secretory precursor cell of mammary gland (CL4033057)
    Fold Change: -0.0713
    Cell Significance Index: -3.3500
  • Cell Name: odontoblast (CL0000060)
    Fold Change: -0.0726
    Cell Significance Index: -9.3100
  • Cell Name: eukaryotic cell (CL0000255)
    Fold Change: -0.0754
    Cell Significance Index: -3.2800
  • Cell Name: pigmented ciliary epithelial cell (CL0002303)
    Fold Change: -0.0757
    Cell Significance Index: -11.0000
  • Cell Name: lactocyte (CL0002325)
    Fold Change: -0.0802
    Cell Significance Index: -10.3600
  • Cell Name: pancreatic ductal cell (CL0002079)
    Fold Change: -0.0838
    Cell Significance Index: -9.6000
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: -0.1008
    Cell Significance Index: -3.2300
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: -0.1154
    Cell Significance Index: -8.8600
  • Cell Name: sebum secreting cell (CL0000317)
    Fold Change: -0.1209
    Cell Significance Index: -8.5500
  • Cell Name: myeloid cell (CL0000763)
    Fold Change: -0.1212
    Cell Significance Index: -1.6100
  • Cell Name: immature innate lymphoid cell (CL0001082)
    Fold Change: -0.1245
    Cell Significance Index: -1.5400
  • Cell Name: progenitor cell of mammary luminal epithelium (CL0009116)
    Fold Change: -0.1249
    Cell Significance Index: -9.3100
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: -0.1329
    Cell Significance Index: -8.1500
  • Cell Name: leukocyte (CL0000738)
    Fold Change: -0.1413
    Cell Significance Index: -2.4000
  • Cell Name: acinar cell of salivary gland (CL0002623)
    Fold Change: -0.1413
    Cell Significance Index: -6.5900
  • Cell Name: eye photoreceptor cell (CL0000287)
    Fold Change: -0.1429
    Cell Significance Index: -9.0100
  • Cell Name: hippocampal granule cell (CL0001033)
    Fold Change: -0.1529
    Cell Significance Index: -10.2800
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: -0.1582
    Cell Significance Index: -4.4200
  • Cell Name: lung endothelial cell (CL1001567)
    Fold Change: -0.1645
    Cell Significance Index: -8.5700
  • Cell Name: forebrain neuroblast (CL1000042)
    Fold Change: -0.1678
    Cell Significance Index: -10.3200
  • Cell Name: Hofbauer cell (CL3000001)
    Fold Change: -0.1692
    Cell Significance Index: -1.3800
  • Cell Name: retinal progenitor cell (CL0002672)
    Fold Change: -0.1784
    Cell Significance Index: -10.0100
  • Cell Name: glycinergic neuron (CL1001509)
    Fold Change: -0.1886
    Cell Significance Index: -9.9000
  • Cell Name: CD4-positive, alpha-beta thymocyte (CL0000810)
    Fold Change: -0.1966
    Cell Significance Index: -3.3900
  • Cell Name: CD14-positive monocyte (CL0001054)
    Fold Change: -0.1974
    Cell Significance Index: -3.8600
  • Cell Name: basal cell of prostate epithelium (CL0002341)
    Fold Change: -0.2006
    Cell Significance Index: -5.4600
  • Cell Name: macrophage (CL0000235)
    Fold Change: -0.2096
    Cell Significance Index: -2.1400
  • Cell Name: indirect pathway medium spiny neuron (CL4023029)
    Fold Change: -0.2243
    Cell Significance Index: -9.9200
  • Cell Name: neuron associated cell (CL0000095)
    Fold Change: -0.2253
    Cell Significance Index: -9.2300
  • Cell Name: proerythroblast (CL0000547)
    Fold Change: -0.2296
    Cell Significance Index: -3.2900
  • Cell Name: stratified epithelial cell (CL0000079)
    Fold Change: -0.2602
    Cell Significance Index: -9.5500
  • Cell Name: direct pathway medium spiny neuron (CL4023026)
    Fold Change: -0.2628
    Cell Significance Index: -9.9500
  • Cell Name: skeletal muscle fiber (CL0008002)
    Fold Change: -0.2661
    Cell Significance Index: -6.8400
  • Cell Name: CD14-low, CD16-positive monocyte (CL0002396)
    Fold Change: -0.2704
    Cell Significance Index: -6.5500
  • Cell Name: L5 extratelencephalic projecting glutamatergic cortical neuron (CL4023041)
    Fold Change: -0.2712
    Cell Significance Index: -9.5000
  • Cell Name: alveolar macrophage (CL0000583)
    Fold Change: -0.2735
    Cell Significance Index: -2.4100
  • Cell Name: lymphoid lineage restricted progenitor cell (CL0000838)
    Fold Change: -0.2762
    Cell Significance Index: -3.5000
  • Cell Name: kidney epithelial cell (CL0002518)
    Fold Change: -0.2781
    Cell Significance Index: -8.1900

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics:** 1. **Cellular localization:** NCF4 is primarily found in the cytosol of neutrophils, macrophages, and other myeloid cells. 2. **Protein structure:** The NCF4 protein is a member of the NADPH oxidase complex, which is composed of multiple subunits, including NCF1, NCF2, NCF4, and p22phox. 3. **Function:** NCF4 is essential for the assembly and activation of the NADPH oxidase complex, which generates superoxide anions in response to various stimuli, such as pathogen recognition, oxidative stress, and inflammatory signals. **Pathways and Functions:** 1. **NADPH oxidase complex:** NCF4 is a critical subunit of the NADPH oxidase complex, which plays a central role in the respiratory burst and superoxide generation in response to pathogens and oxidative stress. 2. **Phagocytosis:** NCF4 is involved in the regulation of phagocytosis, a process by which immune cells engulf and eliminate pathogens. 3. **Antigen processing and presentation:** NCF4 is also involved in the regulation of antigen processing and presentation, which is essential for the activation of T-cells and the initiation of adaptive immune responses. 4. **Cellular responses to stimuli:** NCF4 is activated in response to various stimuli, including pathogens, oxidative stress, and inflammatory signals, leading to the production of reactive oxygen species (ROS) and the activation of downstream signaling pathways. **Clinical Significance:** 1. **Immunodeficiency disorders:** Mutations in the NCF4 gene have been associated with immunodeficiency disorders, such as chronic granulomatous disease (CGD), which is characterized by impaired phagocytosis and oxidative burst. 2. **Inflammatory diseases:** NCF4 has been implicated in the pathogenesis of various inflammatory diseases, including asthma, chronic obstructive pulmonary disease (COPD), and rheumatoid arthritis. 3. **Cancer:** NCF4 has been shown to play a role in cancer progression, including the regulation of tumor growth, invasion, and metastasis. 4. **Neurological disorders:** NCF4 has been implicated in the pathogenesis of neurological disorders, including multiple sclerosis and Alzheimer's disease. In conclusion, the NCF4 gene plays a crucial role in the regulation of immune responses, including phagocytosis, respiratory burst, and superoxide generation. Its dysregulation has been implicated in various immunodeficiency disorders, inflammatory diseases, and cancer. Further research is needed to fully elucidate the mechanisms by which NCF4 regulates immune responses and to explore its potential as a therapeutic target for various diseases.

Genular Protein ID: 1655151888

Symbol: NCF4_HUMAN

Name: Neutrophil cytosol factor 4

UniProtKB Accession Codes:

Q15080 A8K4F9 O60808 Q86U56 Q9BU98 Q9NP45

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 BMRB 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 2 CDD 3 CTD 1 ChEBI 2 ChiTaRS 1 ComplexPortal 3 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 DrugBank 1 EMBL 19 Ensembl 2 EvolutionaryTrace 1 ExpressionAtlas 1 GO 12 Gene3D 2 GeneCards 1 GeneReviews 1 GeneTree 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 IDEAL 1 InParanoid 1 IntAct 1 InterPro 11 KEGG 1 MANE-Select 1 MIM 3 MINT 1 MalaCards 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 Orphanet 1 OrthoDB 1 PANTHER 2 PDB 6 PDBsum 6 PIR 1 PRIDE 1 PRINTS 1 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 3 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 2 RNAct 1 Reactome 8 RefSeq 2 SIGNOR 1 SMART 3 SMR 1 STRING 1 SUPFAM 3 SignaLink 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 8280052

Title: p40phox, a third cytosolic component of the activation complex of the NADPH oxidase to contain src homology 3 domains.

PubMed ID: 8280052

DOI: 10.1042/bj2960557

PubMed ID: 8839867

Title: Genomic structure, chromosomal localization, start of transcription, and tissue expression of the human p40-phox, a new component of the nicotinamide adenine dinucleotide phosphate-oxidase complex.

PubMed ID: 8839867

PubMed ID: 10437784

Title: Identification of a splice variant mRNA of p40phox, an NADPH oxidase component of phagocytes.

PubMed ID: 10437784

DOI: 10.1016/s0014-5793(99)00905-9

PubMed ID: 15461802

Title: A genome annotation-driven approach to cloning the human ORFeome.

PubMed ID: 15461802

DOI: 10.1186/gb-2004-5-10-r84

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 10591208

Title: The DNA sequence of human chromosome 22.

PubMed ID: 10591208

DOI: 10.1038/990031

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 9804763

Title: p40(phox) is phosphorylated on threonine 154 and serine 315 during activation of the phagocyte NADPH oxidase. Implication of a protein kinase c-type kinase in the phosphorylation process.

PubMed ID: 9804763

DOI: 10.1074/jbc.273.46.30097

PubMed ID: 11684018

Title: The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate.

PubMed ID: 11684018

DOI: 10.1016/s1097-2765(01)00372-0

PubMed ID: 12887891

Title: PB1 domain-mediated heterodimerization in NADPH oxidase and signaling complexes of atypical protein kinase C with Par6 and p62.

PubMed ID: 12887891

DOI: 10.1016/s1097-2765(03)00246-6

PubMed ID: 15657040

Title: Effects of p47phox C terminus phosphorylations on binding interactions with p40phox and p67phox. Structural and functional comparison of p40phox and p67phox SH3 domains.

PubMed ID: 15657040

DOI: 10.1074/jbc.m412897200

PubMed ID: 17290225

Title: Full-length p40phox structure suggests a basis for regulation mechanism of its membrane binding.

PubMed ID: 17290225

DOI: 10.1038/sj.emboj.7601561

PubMed ID: 19692703

Title: A new genetic subgroup of chronic granulomatous disease with autosomal recessive mutations in p40 phox and selective defects in neutrophil NADPH oxidase activity.

PubMed ID: 19692703

DOI: 10.1182/blood-2009-07-231498

Sequence Information:

  • Length: 339
  • Mass: 39032
  • Checksum: D82FE9E5BA12890B
  • Sequence:
    • MAVAQQLRAE SDFEQLPDDV AISANIADIE EKRGFTSHFV FVIEVKTKGG SKYLIYRRYR 
QFHALQSKLE ERFGPDSKSS ALACTLPTLP AKVYVGVKQE IAEMRIPALN AYMKSLLSLP 
VWVLMDEDVR IFFYQSPYDS EQVPQALRRL RPRTRKVKSV SPQGNSVDRM AAPRAEALFD 
FTGNSKLELN FKAGDVIFLL SRINKDWLEG TVRGATGIFP LSFVKILKDF PEEDDPTNWL 
RCYYYEDTIS TIKDIAVEED LSSTPLLKDL LELTRREFQR EDIALNYRDA EGDLVRLLSD 
EDVALMVRQA RGLPSQKRLF PWKLHITQKD NYRVYNTMP

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.