Details for: NEDD4

Gene ID: 4734

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: NEDD4

Ensembl ID: ENSG00000069869

Description: NEDD4 E3 ubiquitin protein ligase

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • hepatocyte CL0000182
    CSI 9.23
    rCSI 16.52%
    PRS 72.87
  • blood vessel endothelial cell CL0000071
    CSI 8.46
    rCSI 17.55%
    PRS 70.6
  • stem cell CL0000034
    CSI 8.34
    rCSI 8.05%
    PRS 65.81
  • alveolar adventitial fibroblast CL4028006
    CSI 6.44
    rCSI 10.17%
    PRS 75.44
  • L6b glutamatergic cortical neuron CL4023038
    CSI 6.32
    rCSI 19.76%
    PRS 56.7
  • lung ciliated cell CL1000271
    CSI 6.27
    rCSI 7.25%
    PRS 65
  • cerebral cortex endothelial cell CL1001602
    CSI 6.02
    rCSI 10.42%
    PRS 64.28
  • melanocyte CL0000148
    CSI 5.51
    rCSI 4.08%
    PRS 66.58
  • chandelier pvalb GABAergic cortical interneuron CL4023036
    CSI 5.19
    rCSI 16.24%
    PRS 59.13
  • pulmonary capillary endothelial cell CL4028001
    CSI 4.68
    rCSI 8.92%
    PRS 85.01
  • neural progenitor cell CL0011020
    CSI 4.43
    rCSI 19.48%
    PRS 62.28
  • vascular leptomeningeal cell CL4023051
    CSI 4.28
    rCSI 7.5%
    PRS 66.37
  • renal interstitial pericyte CL1001318
    CSI 4.25
    rCSI 11.72%
    PRS 68.49
  • extravillous trophoblast CL0008036
    CSI 4.08
    rCSI 5.05%
    PRS 70.93
  • keratinocyte CL0000312
    CSI 4.03
    rCSI 3.37%
    PRS 76.73
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 3.48
    rCSI 4.01%
    PRS 65.8
  • hepatic stellate cell CL0000632
    CSI 3.46
    rCSI 12.97%
    PRS 65.63
  • glial cell CL0000125
    CSI 3.22
    rCSI 12.26%
    PRS 64
  • midzonal region hepatocyte CL0019028
    CSI 3.22
    rCSI 7.54%
    PRS 74.98
  • neural crest cell CL0011012
    CSI 3.18
    rCSI 2.51%
    PRS 61.18
  • enterocyte CL0000584
    CSI 3.17
    rCSI 5.12%
    PRS 74.08
  • retinal blood vessel endothelial cell CL0002585
    CSI 3.14
    rCSI 5.01%
    PRS 77.35
  • fibroblast of lung CL0002553
    CSI 3.11
    rCSI 2.89%
    PRS 74.36
  • cardiac neuron CL0010022
    CSI 2.95
    rCSI 9.45%
    PRS 70.68
  • epithelial cell of lower respiratory tract CL0002632
    CSI 2.94
    rCSI 2.28%
    PRS 76.9
  • alveolar type 1 fibroblast cell CL4028004
    CSI 2.92
    rCSI 3.2%
    PRS 76.3
  • Kupffer cell CL0000091
    CSI 2.89
    rCSI 6.61%
    PRS 74.21
  • adipocyte CL0000136
    CSI 2.85
    rCSI 3.66%
    PRS 64.25
  • differentiation-committed oligodendrocyte precursor CL4023059
    CSI 2.75
    rCSI 5%
    PRS 64.56
  • kidney loop of Henle thin descending limb epithelial cell CL1001111
    CSI 2.73
    rCSI 3.86%
    PRS 69.91
  • mesothelial cell CL0000077
    CSI 2.7
    rCSI 10.56%
    PRS 50.87
  • sst GABAergic cortical interneuron CL4023017
    CSI 2.67
    rCSI 3.44%
    PRS 56.25
  • interstitial cell of Cajal CL0002088
    CSI 2.62
    rCSI 3.33%
    PRS 79.13
  • retinal cone cell CL0000573
    CSI 2.58
    rCSI 4.15%
    PRS 63.12
  • enteric smooth muscle cell CL0002504
    CSI 2.54
    rCSI 3.62%
    PRS 75.03
  • kidney connecting tubule epithelial cell CL1000768
    CSI 2.45
    rCSI 6.21%
    PRS 63.44
  • astrocyte of the cerebral cortex CL0002605
    CSI 2.44
    rCSI 5.47%
    PRS 55.54
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 2.44
    rCSI 3.03%
    PRS 52.91
  • epithelial cell of proximal tubule CL0002306
    CSI 2.42
    rCSI 5.91%
    PRS 66.45
  • fast muscle cell CL0000190
    CSI 2.4
    rCSI 9.39%
    PRS 71.61
  • endothelial cell of vascular tree CL0002139
    CSI 2.35
    rCSI 12.85%
    PRS 70.89
  • lung secretory cell CL1000272
    CSI 2.34
    rCSI 5.78%
    PRS 72.77
  • Mueller cell CL0000636
    CSI 2.32
    rCSI 5.3%
    PRS 64.79
  • endothelial cell of pericentral hepatic sinusoid CL0019022
    CSI 2.27
    rCSI 6.98%
    PRS 79.07
  • parietal epithelial cell CL1000452
    CSI 2.2
    rCSI 5.89%
    PRS 64.63
  • intrahepatic cholangiocyte CL0002538
    CSI 2.2
    rCSI 5.28%
    PRS 79.63
  • kidney interstitial alternatively activated macrophage CL1000695
    CSI 2.2
    rCSI 5.73%
    PRS 73.98
  • S cone cell CL0003050
    CSI 2.19
    rCSI 9.61%
    PRS 69.32
  • retinal rod cell CL0000604
    CSI 2.18
    rCSI 3.85%
    PRS 69.67
  • intestinal crypt stem cell of small intestine CL0009017
    CSI 2.13
    rCSI 5.74%
    PRS 79.58
  • VIP GABAergic cortical interneuron CL4023016
    CSI 2.06
    rCSI 2.46%
    PRS 54.79
  • enteroglial cell CL4040002
    CSI 2.06
    rCSI 10.84%
    PRS 76.32
  • pulmonary artery endothelial cell CL1001568
    CSI 2.05
    rCSI 2.78%
    PRS 83.28
  • fibroblast of cardiac tissue CL0002548
    CSI 1.99
    rCSI 9.52%
    PRS 73.47
  • hematopoietic multipotent progenitor cell CL0000837
    CSI 1.95
    rCSI 4.68%
    PRS 88.06
  • endocardial cell CL0002350
    CSI 1.91
    rCSI 9.13%
    PRS 70.93
  • Schwann cell CL0002573
    CSI 1.84
    rCSI 5.24%
    PRS 70.12
  • chondrocyte CL0000138
    CSI 1.74
    rCSI 2.77%
    PRS 66.18
  • placental villous trophoblast CL2000060
    CSI 1.7
    rCSI 2.62%
    PRS 72.6
  • lung pericyte CL0009089
    CSI 1.53
    rCSI 4.03%
    PRS 81.63
  • tracheobronchial smooth muscle cell CL0019019
    CSI 1.53
    rCSI 2.69%
    PRS 80.19
  • small intestine goblet cell CL1000495
    CSI 1.46
    rCSI 3.2%
    PRS 80.03
  • endothelial cell of placenta CL0009092
    CSI 1.42
    rCSI 7.01%
    PRS 83.06
  • cardiac endothelial cell CL0010008
    CSI 1.39
    rCSI 5.59%
    PRS 72.84
  • keratocyte CL0002363
    CSI 1.16
    rCSI 2.79%
    PRS 78.33
  • cardiac blood vessel endothelial cell CL0010006
    CSI 0.84
    rCSI 5.91%
    PRS 66.45
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 0.79
    rCSI 2.86%
    PRS 53.02
  • blood vessel smooth muscle cell CL0019018
    CSI 0.66
    rCSI 5.37%
    PRS 67.28
  • stromal cell CL0000499
    CSI 0.62
    rCSI 1.74%
    PRS 69.04

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [NEDD4](/details-gene/4734) (Neural precursor cell expressed, developmentally down-regulated 4) is an E3 ubiquitin-protein ligase that plays a critical role in protein turnover and signal transduction. As a member of the HECT domain family of E3 ligases, it targets a wide array of substrate proteins for ubiquitination, leading to their degradation, internalization, or altered function. Functionally, [NEDD4](/details-gene/4734) is implicated in numerous cellular processes, including '[receptor internalization](/details-gene/GO:0031623)', '[lysosomal transport](/details-gene/GO:0007041)', '[innate immune response](/details-gene/GO:0045087)', and the regulation of ion channel activity. It is also known for its involvement in pathways critical for cell growth and survival, such as '[Pten regulation](/details-gene/R-HSA-6807070)' and signaling by receptor tyrosine kinases. Reflecting its fundamental roles, [NEDD4](/details-gene/4734) shows significant expression across a diverse range of cell types, with particularly high significance in metabolically active cells like [hepatocytes](/details-cell/CL0000182) and cells forming critical barriers, such as [blood vessel endothelial cells](/details-cell/CL0000071). Its role in human health is underscored by its association with developmental processes and its hijacking by various viruses to facilitate budding ([Link](https://doi.org/10.1016/s0022-2836(02)01406-7)). ## Cellular Roles and Expression Landscape The expression profile of [NEDD4](/details-gene/4734) suggests it functions as a key enzymatic component in a broad spectrum of cell types rather than as a specific lineage marker. **Overall**, its high Cell Significance Index (CSI) in diverse cell types highlights its widespread importance in cellular homeostasis and signaling. The most significant expression is observed in cell types characterized by high metabolic activity, complex signaling, or barrier functions. Its top ranking in [hepatocytes](/details-cell/CL0000182) (CSI: 9.23) is consistent with a role in regulating protein turnover and signaling in the liver, a central metabolic hub. Similarly, high significance in various endothelial cells, including [blood vessel endothelial cell](/details-cell/CL0000071) (CSI: 8.46), [cerebral cortex endothelial cell](/details-cell/CL1001602) (CSI: 6.02), and [pulmonary capillary endothelial cell](/details-cell/CL4028001) (CSI: 4.68), points to its involvement in maintaining vascular integrity and regulating transport and signaling across the endothelium. Furthermore, [NEDD4](/details-gene/4734) appears crucial for progenitor and structural cells, as indicated by its high significance in [stem cells](/details-cell/CL0000034) (CSI: 8.34), [alveolar adventitial fibroblasts](/details-cell/CL4028006) (CSI: 6.44), and [keratinocytes](/details-cell/CL0000312) (CSI: 4.03). Its notable significance in neuronal subtypes, such as [L6b glutamatergic cortical neurons](/details-cell/CL4023038) (CSI: 6.32) and [chandelier pvalb GABAergic cortical interneurons](/details-cell/CL4023036) (CSI: 5.19), aligns with its annotated functions in '[neuron projection development](/details-gene/GO:0031175)' and '[regulation of synapse organization](/details-gene/GO:0050807)'. This broad but high-level expression pattern establishes [NEDD4](/details-gene/4734) as a vital ubiquitin ligase essential for the function of multiple major organ systems. ## Pathways and Molecular Function The primary molecular function of [NEDD4](/details-gene/4734) is its '[ubiquitin protein ligase activity](/details-gene/GO:0061630)', through which it catalyzes the attachment of ubiquitin to substrate proteins. This core activity drives its participation in a vast range of biological processes. A key role is in protein degradation via the '[ubiquitin-dependent protein catabolic process](/details-gene/GO:0006511)', including sorting proteins into the multivesicular body pathway for lysosomal degradation. This is central to its function in downregulating cell surface receptors, as described in '[receptor internalization](/details-gene/GO:0031623)' and '[receptor catabolic process](/details-gene/GO:0032801)'. Its role as a master regulator of signaling pathways is particularly evident from its involvement in the '[Pten regulation](/details-gene/R-HSA-6807070)' pathway. Seminal research identified [NEDD4](/details-gene/4734) as a proto-oncogenic ligase that targets the tumor suppressor PTEN for degradation, thereby promoting PI3K/AKT signaling ([Link](https://doi.org/10.1016/j.cell.2006.11.039)), although this finding has been contested, suggesting the regulation may be context-dependent ([Link](https://doi.org/10.1073/pnas.0803233105)). This function connects directly to pathways like '[PIP3 activates AKT signaling](/details-gene/R-HSA-1257604)' and '[Signaling by receptor tyrosine kinases](/details-gene/R-HSA-9006934)'. Additionally, [NEDD4](/details-gene/4734) plays a significant role in the immune system and host-pathogen interactions. It is annotated in the '[innate immune response](/details-gene/GO:0045087)' and is a component of the '[interferon signaling](/details-gene/R-HSA-913531)' pathway. Critically, its function is co-opted by several viruses, including Ebola virus and Herpes simplex virus, to facilitate '[viral budding](/details-gene/GO:0046755)' from host cells, making it a key host factor in viral pathogenesis ([Link](https://doi.org/10.1016/s0022-2836(02)01406-7)), ([Link](https://doi.org/10.1128/jvi.02515-07)). ## Research Directions The widespread expression and multi-faceted roles of [NEDD4](/details-gene/4734) present several avenues for future investigation, particularly regarding its regulatory specificity and therapeutic potential. **Proposed Hypotheses:** 1. The conflicting reports on [NEDD4](/details-gene/4734)-mediated degradation of the tumor suppressor PTEN suggest that this interaction is highly context-specific. We hypothesize that the ubiquitination of PTEN by [NEDD4](/details-gene/4734) is dependent on cell-type-specific adaptor proteins or post-translational modifications on either [NEDD4](/details-gene/4734) or PTEN, which are present in certain cancer cells but absent in others. 2. Given its role in the budding of multiple, unrelated enveloped viruses (e.g., Ebola, HTLV-1, HSV), we hypothesize that [NEDD4](/details-gene/4734) is a central host hub for viral egress. Its inhibition may therefore represent a broad-spectrum antiviral strategy against viruses that utilize the host ESCRT machinery for their release. **Experimental Approach:** To test the first hypothesis regarding context-specific PTEN regulation, a combination of proteomic and genetic approaches could be employed. Co-immunoprecipitation of endogenous [NEDD4](/details-gene/4734) followed by mass spectrometry could be performed in cell lines where the [NEDD4](/details-gene/4734)-PTEN interaction has been reported versus those where it has been refuted. This would identify candidate adaptor proteins or differential post-translational modifications. Subsequently, CRISPR-Cas9 could be used to knock out these candidate adaptors in the relevant cell lines to determine if their absence abrogates [NEDD4](/details-gene/4734)-mediated PTEN degradation and the subsequent activation of AKT signaling. **Therapeutic Potential:** [NEDD4](/details-gene/4734)'s demonstrated role in degrading tumor suppressors like PTEN positions it as a potential therapeutic target in oncology. **Inhibition**, rather than activation, would be the therapeutic goal to stabilize tumor suppressors and curb pro-survival signaling. However, its broad expression and essential roles in healthy tissues, such as in [hepatocytes](/details-cell/CL0000182) and [endothelial cells](/details-cell/CL0000071), pose a major challenge for systemic inhibition due to the high risk of on-target toxicity. A viable therapeutic strategy would likely require the development of small molecule inhibitors that specifically disrupt the interaction of [NEDD4](/details-gene/4734) with its oncogenic substrates while sparing its housekeeping functions, or the use of targeted delivery systems (e.g., antibody-drug conjugates) to deliver a [NEDD4](/details-gene/4734) inhibitor specifically to cancer cells.

Genular Protein ID: 1954174065

Symbol: NEDD4_HUMAN

Name: E3 ubiquitin-protein ligase NEDD4

UniProtKB Accession Codes:

P46934 A1KY35 A6ND72 A7MD29 B4E2R7 B7ZM59 B7ZM60 B9EGN5 D6RF89

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 BRENDA 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 4 CDD 2 CORUM 1 CPTC 1 CTD 1 ChEMBL 1 ChiTaRS 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 EC 1 ELM 1 EMBL 12 Ensembl 4 EvolutionaryTrace 1 ExpressionAtlas 1 GO 54 Gene3D 5 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 6 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 13 PDBsum 13 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 4 Pumba 1 RNAct 1 Reactome 5 RefSeq 6 SIGNOR 1 SMART 2 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 TCDB 1 TreeFam 1 UCSC 1 UniPathway 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 17218260

Title: NEDD4-1 is a proto-oncogenic ubiquitin ligase for PTEN.

PubMed ID: 17218260

DOI: 10.1016/j.cell.2006.11.039

PubMed ID: 7788527

Title: Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1.

PubMed ID: 7788527

DOI: 10.1093/dnares/2.1.37

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 17974005

Title: The full-ORF clone resource of the German cDNA consortium.

PubMed ID: 17974005

DOI: 10.1186/1471-2164-8-399

PubMed ID: 16572171

Title: Analysis of the DNA sequence and duplication history of human chromosome 15.

PubMed ID: 16572171

DOI: 10.1038/nature04601

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 11342538

Title: Nuclear import/export of hRPF1/Nedd4 regulates the ubiquitin-dependent degradation of its nuclear substrates.

PubMed ID: 11342538

DOI: 10.1074/jbc.m101205200

PubMed ID: 11598133

Title: Nedd4 regulates ubiquitination and stability of the guanine-nucleotide exchange factor CNrasGEF.

PubMed ID: 11598133

DOI: 10.1074/jbc.m108373200

PubMed ID: 12559917

Title: Ebola virus matrix protein VP40 interaction with human cellular factors Tsg101 and Nedd4.

PubMed ID: 12559917

DOI: 10.1016/s0022-2836(02)01406-7

PubMed ID: 14581525

Title: PPPYVEPTAP motif is the late domain of human T-cell leukemia virus type 1 Gag and mediates its functional interaction with cellular proteins Nedd4 and Tsg101.

PubMed ID: 14581525

DOI: 10.1128/jvi.77.22.11882-11895.2003

PubMed ID: 18819914

Title: Nedd4 family-interacting protein 1 (Ndfip1) is required for the exosomal secretion of Nedd4 family proteins.

PubMed ID: 18819914

DOI: 10.1074/jbc.m804120200

PubMed ID: 18353951

Title: Herpes simplex virus type 2 UL56 interacts with the ubiquitin ligase Nedd4 and increases its ubiquitination.

PubMed ID: 18353951

DOI: 10.1128/jvi.02515-07

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 18305167

Title: ISG15 inhibits Ebola VP40 VLP budding in an L-domain-dependent manner by blocking Nedd4 ligase activity.

PubMed ID: 18305167

DOI: 10.1073/pnas.0710629105

PubMed ID: 18562292

Title: The ubiquitin ligase Nedd4-1 is dispensable for the regulation of PTEN stability and localization.

PubMed ID: 18562292

DOI: 10.1073/pnas.0803233105

PubMed ID: 19920177

Title: Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation.

PubMed ID: 19920177

DOI: 10.1073/pnas.0907052106

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 20559325

Title: Arrestin domain-containing protein 3 recruits the NEDD4 E3 ligase to mediate ubiquitination of the beta2-adrenergic receptor.

PubMed ID: 20559325

DOI: 10.1038/embor.2010.80

PubMed ID: 20086093

Title: HECT E3 ubiquitin ligase Nedd4-1 ubiquitinates ACK and regulates epidermal growth factor (EGF)-induced degradation of EGF receptor and ACK.

PubMed ID: 20086093

DOI: 10.1128/mcb.00013-10

PubMed ID: 20159449

Title: Regulation of Rap2A by the ubiquitin ligase Nedd4-1 controls neurite development.

PubMed ID: 20159449

DOI: 10.1016/j.neuron.2010.01.007

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 21765395

Title: Nedd4-1 binds and ubiquitylates activated FGFR1 to control its endocytosis and function.

PubMed ID: 21765395

DOI: 10.1038/emboj.2011.234

PubMed ID: 21191027

Title: Multiple interactions between the ESCRT machinery and arrestin-related proteins: implications for PPXY-dependent budding.

PubMed ID: 21191027

DOI: 10.1128/jvi.02045-10

PubMed ID: 22096579

Title: A role for the ubiquitin ligase Nedd4 in membrane sorting of LAPTM4 proteins.

PubMed ID: 22096579

DOI: 10.1371/journal.pone.0027478

PubMed ID: 23166351

Title: MARCH2 promotes endocytosis and lysosomal sorting of carvedilol-bound beta(2)-adrenergic receptors.

PubMed ID: 23166351

DOI: 10.1083/jcb.201208192

PubMed ID: 22223895

Title: Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features.

PubMed ID: 22223895

DOI: 10.1074/mcp.m111.015131

PubMed ID: 22179831

Title: Deubiquitination of EGFR by Cezanne-1 contributes to cancer progression.

PubMed ID: 22179831

DOI: 10.1038/onc.2011.587

PubMed ID: 23873930

Title: Cellular localization and characterization of cytosolic binding partners for Gla domain-containing proteins PRRG4 and PRRG2.

PubMed ID: 23873930

DOI: 10.1074/jbc.m113.484683

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 25631046

Title: Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response.

PubMed ID: 25631046

DOI: 10.1074/jbc.m114.613687

PubMed ID: 26854353

Title: Regulation of Itch and Nedd4 E3 Ligase Activity and Degradation by LRAD3.

PubMed ID: 26854353

DOI: 10.1021/acs.biochem.5b01218

PubMed ID: 32101753

Title: Auto-ubiquitination of NEDD4-1 Recruits USP13 to Facilitate Autophagy through Deubiquitinating VPS34.

PubMed ID: 32101753

DOI: 10.1016/j.celrep.2020.01.088

PubMed ID: 21399620

Title: Structure of the HECT:ubiquitin complex and its role in ubiquitin chain elongation.

PubMed ID: 21399620

DOI: 10.1038/embor.2011.21

PubMed ID: 23665454

Title: Structure and dynamics of human Nedd4-1 WW3 in complex with the alphaENaC PY motif.

PubMed ID: 23665454

DOI: 10.1016/j.bbapap.2013.04.031

PubMed ID: 23644597

Title: Structure of a ubiquitin-loaded HECT ligase reveals the molecular basis for catalytic priming.

PubMed ID: 23644597

DOI: 10.1038/nsmb.2566

PubMed ID: 24379409

Title: Structural and biochemical basis for ubiquitin ligase recruitment by arrestin-related domain-containing protein-3 (ARRDC3).

PubMed ID: 24379409

DOI: 10.1074/jbc.m113.527473

PubMed ID: 16959974

Title: The consensus coding sequences of human breast and colorectal cancers.

PubMed ID: 16959974

DOI: 10.1126/science.1133427

Sequence Information:

  • Length: 1319
  • Mass: 149114
  • Checksum: D56EBBC50A34F13B
  • Sequence:
    • MAQSLRLHFA ARRSNTYPLS ETSGDDLDSH VHMCFKRPTR ISTSNVVQMK LTPRQTALAP 
LIKENVQSQE RSSVPSSENV NKKSSCLQIS LQPTRYSGYL QSSNVLADSD DASFTCILKD 
GIYSSAVVDN ELNAVNDGHL VSSPAICSGS LSNFSTSDNG SYSSNGSDFG SCASITSGGS 
YTNSVISDSS SYTFPPSDDT FLGGNLPSDS TSNRSVPNRN TTPCEIFSRS TSTDPFVQDD 
LEHGLEIMKL PVSRNTKIPL KRYSSLVIFP RSPSTTRPTS PTSLCTLLSK GSYQTSHQFI 
ISPSEIAHNE DGTSAKGFLS TAVNGLRLSK TICTPGEVRD IRPLHRKGSL QKKIVLSNNT 
PRQTVCEKSS EGYSCVSVHF TQRKAATLDC ETTNGDCKPE MSEIKLNSDS EYIKLMHRTS 
ACLPSSQNVD CQININGELE RPHSQMNKNH GILRRSISLG GAYPNISCLS SLKHNCSKGG 
PSQLLIKFAS GNEGKVDNLS RDSNRDCTNE LSNSCKTRDD FLGQVDVPLY PLPTENPRLE 
RPYTFKDFVL HPRSHKSRVK GYLRLKMTYL PKTSGSEDDN AEQAEELEPG WVVLDQPDAA 
CHLQQQQEPS PLPPGWEERQ DILGRTYYVN HESRRTQWKR PTPQDNLTDA ENGNIQLQAQ 
RAFTTRRQIS EETESVDNRE SSENWEIIRE DEATMYSNQA FPSPPPSSNL DVPTHLAEEL 
NARLTIFGNS AVSQPASSSN HSSRRGSLQA YTFEEQPTLP VLLPTSSGLP PGWEEKQDER 
GRSYYVDHNS RTTTWTKPTV QATVETSQLT SSQSSAGPQS QASTSDSGQQ VTQPSEIEQG 
FLPKGWEVRH APNGRPFFID HNTKTTTWED PRLKIPAHLR GKTSLDTSND LGPLPPGWEE 
RTHTDGRIFY INHNIKRTQW EDPRLENVAI TGPAVPYSRD YKRKYEFFRR KLKKQNDIPN 
KFEMKLRRAT VLEDSYRRIM GVKRADFLKA RLWIEFDGEK GLDYGGVARE WFFLISKEMF 
NPYYGLFEYS ATDNYTLQIN PNSGLCNEDH LSYFKFIGRV AGMAVYHGKL LDGFFIRPFY 
KMMLHKPITL HDMESVDSEY YNSLRWILEN DPTELDLRFI IDEELFGQTH QHELKNGGSE 
IVVTNKNKKE YIYLVIQWRF VNRIQKQMAA FKEGFFELIP QDLIKIFDEN ELELLMCGLG 
DVDVNDWREH TKYKNGYSAN HQVIQWFWKA VLMMDSEKRI RLLQFVTGTS RVPMNGFAEL 
YGSNGPQSFT VEQWGTPEKL PRAHTCFNRL DLPPYESFEE LWDKLQMAIE NTQGFDGVD