Details for: MED1

Gene ID: 5469

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: MED1

Ensembl ID: ENSG00000125686

Description: mediator complex subunit 1

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • multi-ciliated epithelial cell CL0005012
    CSI 5.89
    rCSI 5.88%
    PRS 63.29
  • astrocyte of the cerebral cortex CL0002605
    CSI 5.75
    rCSI 12.89%
    PRS 51.68
  • mature B cell CL0000785
    CSI 4.24
    rCSI 3.69%
    PRS 80.25
  • VIP GABAergic cortical interneuron CL4023016
    CSI 4.11
    rCSI 4.91%
    PRS 50.79
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 3.97
    rCSI 7.01%
    PRS 50.1
  • intermediate monocyte CL0002393
    CSI 3.82
    rCSI 5.76%
    PRS 74.86
  • retinal pigment epithelial cell CL0002586
    CSI 3.69
    rCSI 7.33%
    PRS 66.37
  • double negative thymocyte CL0002489
    CSI 3.53
    rCSI 2.45%
    PRS 81.21
  • regular ventricular cardiac myocyte CL0002131
    CSI 3.5
    rCSI 21.87%
    PRS 61.15
  • central memory CD4-positive, alpha-beta T cell CL0000904
    CSI 3.49
    rCSI 2.06%
    PRS 86.07
  • unswitched memory B cell CL0000970
    CSI 3.45
    rCSI 2.9%
    PRS 84.93
  • effector CD8-positive, alpha-beta T cell CL0001050
    CSI 3.39
    rCSI 2.58%
    PRS 82.48
  • mesodermal cell CL0000222
    CSI 3.15
    rCSI 3.78%
    PRS 68.13
  • alpha-beta T cell CL0000789
    CSI 3.11
    rCSI 3.65%
    PRS 84.82
  • pancreatic D cell CL0000173
    CSI 3.1
    rCSI 3.05%
    PRS 72.42
  • dendritic cell, human CL0001056
    CSI 3.01
    rCSI 4.63%
    PRS 79.02
  • plasmacytoid dendritic cell, human CL0001058
    CSI 2.98
    rCSI 2.08%
    PRS 72.7
  • class switched memory B cell CL0000972
    CSI 2.9
    rCSI 2.16%
    PRS 84.72
  • pancreatic A cell CL0000171
    CSI 2.85
    rCSI 2.99%
    PRS 73.35
  • ionocyte CL0005006
    CSI 2.74
    rCSI 2.94%
    PRS 70.13
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 2.72
    rCSI 4.57%
    PRS 51.01
  • interneuron CL0000099
    CSI 2.65
    rCSI 5.33%
    PRS 59.02
  • naive T cell CL0000898
    CSI 2.65
    rCSI 1.85%
    PRS 84.46
  • melanocyte CL0000148
    CSI 2.6
    rCSI 1.92%
    PRS 62.55
  • neural crest cell CL0011012
    CSI 2.58
    rCSI 2.04%
    PRS 57.01
  • pro-B cell CL0000826
    CSI 2.55
    rCSI 2.11%
    PRS 72.23
  • central memory CD8-positive, alpha-beta T cell CL0000907
    CSI 2.48
    rCSI 1.67%
    PRS 82.97
  • granulocyte monocyte progenitor cell CL0000557
    CSI 2.38
    rCSI 2.06%
    PRS 74.5
  • hematopoietic stem cell CL0000037
    CSI 2.38
    rCSI 1.58%
    PRS 72.66
  • pulmonary ionocyte CL0017000
    CSI 2.36
    rCSI 2.88%
    PRS 77.07
  • hepatocyte CL0000182
    CSI 2.33
    rCSI 4.18%
    PRS 69.15
  • CD4-positive, alpha-beta memory T cell CL0000897
    CSI 2.3
    rCSI 1.65%
    PRS 83.21
  • myeloid leukocyte CL0000766
    CSI 2.29
    rCSI 2.12%
    PRS 71.51
  • perivascular cell CL4033054
    CSI 2.23
    rCSI 3.04%
    PRS 75.52
  • immature B cell CL0000816
    CSI 2.22
    rCSI 1.65%
    PRS 82.36
  • kidney loop of Henle thin descending limb epithelial cell CL1001111
    CSI 2.19
    rCSI 3.1%
    PRS 65.96
  • precursor B cell CL0000817
    CSI 2.17
    rCSI 1.9%
    PRS 78.43
  • common myeloid progenitor CL0000049
    CSI 2.16
    rCSI 1.75%
    PRS 71.6
  • CD8-positive, alpha-beta cytotoxic T cell CL0000794
    CSI 2.11
    rCSI 2.52%
    PRS 87.14
  • choroid plexus epithelial cell CL0000706
    CSI 2.1
    rCSI 3.45%
    PRS 58.81
  • pulmonary capillary endothelial cell CL4028001
    CSI 2.08
    rCSI 3.96%
    PRS 82.57
  • retinal bipolar neuron CL0000748
    CSI 2.02
    rCSI 3.79%
    PRS 57.59
  • group 3 innate lymphoid cell CL0001071
    CSI 2.01
    rCSI 1.51%
    PRS 75.57
  • ependymal cell CL0000065
    CSI 1.96
    rCSI 3.98%
    PRS 48.06
  • epithelial cell of lung CL0000082
    CSI 1.95
    rCSI 1.62%
    PRS 69.71
  • lung ciliated cell CL1000271
    CSI 1.92
    rCSI 2.22%
    PRS 60.73
  • fibroblast of lung CL0002553
    CSI 1.88
    rCSI 1.75%
    PRS 70.6
  • early lymphoid progenitor CL0000936
    CSI 1.88
    rCSI 1.65%
    PRS 75.14
  • ciliated epithelial cell CL0000067
    CSI 1.87
    rCSI 1.65%
    PRS 57.78
  • respiratory suprabasal cell CL4033048
    CSI 1.87
    rCSI 2.39%
    PRS 74.08
  • CD14-low, CD16-positive monocyte CL0002396
    CSI 1.69
    rCSI 1.3%
    PRS 71.48
  • stem cell CL0000034
    CSI 1.66
    rCSI 1.6%
    PRS 61.43
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 1.65
    rCSI 2.06%
    PRS 49.05
  • enteroendocrine cell CL0000164
    CSI 1.61
    rCSI 2.19%
    PRS 70.78
  • kidney loop of Henle thin ascending limb epithelial cell CL1001107
    CSI 1.6
    rCSI 4.14%
    PRS 64.65
  • sst GABAergic cortical interneuron CL4023017
    CSI 1.5
    rCSI 1.94%
    PRS 52.33
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 1.48
    rCSI 1.9%
    PRS 66.44
  • chondrocyte CL0000138
    CSI 1.48
    rCSI 2.35%
    PRS 62.43
  • extravillous trophoblast CL0008036
    CSI 1.47
    rCSI 1.82%
    PRS 66.73
  • placental villous trophoblast CL2000060
    CSI 1.47
    rCSI 2.27%
    PRS 68.52
  • CD1c-positive myeloid dendritic cell CL0002399
    CSI 1.45
    rCSI 1.76%
    PRS 78.79
  • epithelial cell of proximal tubule CL0002306
    CSI 1.42
    rCSI 3.46%
    PRS 62.82
  • peripheral nervous system neuron CL2000032
    CSI 1.4
    rCSI 1.91%
    PRS 61.26
  • common dendritic progenitor CL0001029
    CSI 1.38
    rCSI 1.73%
    PRS 79.79
  • basal cell CL0000646
    CSI 1.3
    rCSI 1.74%
    PRS 69.61
  • kidney connecting tubule epithelial cell CL1000768
    CSI 1.28
    rCSI 3.25%
    PRS 59.36
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 1.24
    rCSI 1.12%
    PRS 67.09
  • cardiac muscle cell CL0000746
    CSI 1.22
    rCSI 1.75%
    PRS 59.23
  • sncg GABAergic cortical interneuron CL4023015
    CSI 1.19
    rCSI 1.92%
    PRS 52.94
  • retinal cone cell CL0000573
    CSI 1.12
    rCSI 1.8%
    PRS 59.2
  • vascular associated smooth muscle cell CL0000359
    CSI 1.1
    rCSI 3.58%
    PRS 69.1
  • lymphoid lineage restricted progenitor cell CL0000838
    CSI 1.08
    rCSI 4.2%
    PRS 87.89
  • respiratory basal cell CL0002633
    CSI 1
    rCSI 1.03%
    PRS 75.46
  • amacrine cell CL0000561
    CSI 0.85
    rCSI 2.46%
    PRS 59.07
  • medium spiny neuron CL1001474
    CSI 0.7
    rCSI 6.03%
    PRS 56.96
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 0.7
    rCSI 1.69%
    PRS 49.41
  • podocyte CL0000653
    CSI 0.65
    rCSI 2.88%
    PRS 70.02
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 0.63
    rCSI 2.26%
    PRS 49.16
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 0.6
    rCSI 2.27%
    PRS 51.57
  • GABAergic neuron CL0000617
    CSI 0.59
    rCSI 1.99%
    PRS 54.37
  • chandelier pvalb GABAergic cortical interneuron CL4023036
    CSI 0.53
    rCSI 1.66%
    PRS 55.22
  • effector memory CD8-positive, alpha-beta T cell, terminally differentiated CL0001062
    CSI 0.51
    rCSI 2.54%
    PRS 82.13
  • L6b glutamatergic cortical neuron CL4023038
    CSI 0.46
    rCSI 1.44%
    PRS 52.76
  • blood vessel smooth muscle cell CL0019018
    CSI 0.41
    rCSI 3.32%
    PRS 63.22
  • erythroid progenitor cell CL0000038
    CSI 0.31
    rCSI 1.8%
    PRS 77.49

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [MED1](/details-gene/5469) (Mediator Complex Subunit 1) is a protein-coding gene located on chromosome 17q12. It encodes a key component of the Mediator complex, a large transcriptional co-regulatory assembly that bridges DNA-binding transcription factors with the core RNA polymerase II machinery. Functionally, [MED1](/details-gene/5469) is critical for transmitting signals from nuclear receptors, including those for estrogen, thyroid hormone, vitamin D, and androgens, to regulate gene expression. Consistent with its fundamental role in transcription, its expression is significant across a wide array of cell types, including [multi-ciliated epithelial cells](/details-cell/CL0005012), [astrocytes of the cerebral cortex](/details-cell/CL0002605), and various immune cells, suggesting it is a ubiquitously important factor in cellular development, differentiation, and homeostasis. ## Cellular Roles and Expression Landscape The expression profile of [MED1](/details-gene/5469) highlights its role as a fundamental and broadly active transcriptional regulator rather than a marker for a specific cell lineage. **Overall**, it demonstrates high significance in diverse and functionally distinct cell populations. Its highest significance is observed in [multi-ciliated epithelial cells](/details-cell/CL0005012) (CSI: 5.89), suggesting a critical role in the transcriptional programs governing the function of mucosal surfaces. High significance is also noted in the central nervous system, specifically in [astrocytes of the cerebral cortex](/details-cell/CL0002605) (CSI: 5.75) and various GABAergic interneurons, pointing towards its involvement in regulating glial function and neuronal development. Furthermore, [MED1](/details-gene/5469) appears to be a key player in the immune system, with significant expression in both the adaptive and innate branches. It is highly ranked in [mature B cells](/details-cell/CL0000785) (CSI: 4.24), [unswitched memory B cells](/details-cell/CL0000970) (CSI: 3.45), various T cell subsets such as [central memory CD4-positive, alpha-beta T cells](/details-cell/CL0000904) (CSI: 3.49), and myeloid cells like [intermediate monocytes](/details-cell/CL0002393) (CSI: 3.82). This broad expression pattern across immune lineages is consistent with its function as a central coactivator required for the complex transcriptional changes that underlie immune cell differentiation and activation. ## Pathways and Molecular Function [MED1](/details-gene/5469) functions primarily as a [nuclear receptor coactivator](/details-cell/GO:0030374) ([GO:0030374](https://www.ebi.ac.uk/QuickGO/term/GO:0030374)) within the [Mediator complex](/details-cell/GO:0016592) ([GO:0016592](https://www.ebi.ac.uk/QuickGO/term/GO:0016592)). Its protein product interacts directly with the ligand-binding domains of nuclear receptors in a hormone-dependent manner, as documented in several studies ([Link](https://doi.org/10.1073/pnas.95.14.7939), [Link](https://doi.org/10.1128/mcb.20.8.2718-2726.2000)). This is reflected in its extensive annotation for binding to receptors for thyroid hormone ([GO:0046966](https://www.ebi.ac.uk/QuickGO/term/GO:0046966)), vitamin D ([GO:0042809](https://www.ebi.ac.uk/QuickGO/term/GO:0042809)), estrogen ([GO:0030331](https://www.ebi.ac.uk/QuickGO/term/GO:0030331)), and peroxisome proliferator-activated receptors (PPARs) ([GO:0042975](https://www.ebi.ac.uk/QuickGO/term/GO:0042975)). This core molecular function drives its participation in a vast array of biological processes. It is integral to the [Nuclear receptor transcription pathway](/details-cell/R-HSA-383280) ([R-HSA-383280](https://reactome.org/content/detail/R-HSA-383280)) and [Signaling by nuclear receptors](/details-cell/R-HSA-9006931) ([R-HSA-9006931](https://reactome.org/content/detail/R-HSA-9006931)). This role connects it to critical physiological pathways, including [ESR-mediated signaling](/details-cell/R-HSA-8939211) ([R-HSA-8939211](https://reactome.org/content/detail/R-HSA-8939211)), [Regulation of lipid metabolism by PPARalpha](/details-cell/R-HSA-400206) ([R-HSA-400206](https://reactome.org/content/detail/R-HSA-400206)), and [Adipogenesis](/details-cell/R-HSA-9843745) ([R-HSA-9843745](https://reactome.org/content/detail/R-HSA-9843745)). Its requirement for PPARγ2-stimulated adipogenesis has been experimentally confirmed ([Link](https://doi.org/10.1038/417563a)). Additionally, it is implicated in numerous developmental programs, such as [brain development](/details-cell/GO:0007420) ([GO:0007420](https://www.ebi.ac.uk/QuickGO/term/GO:0007420)), [erythrocyte development](/details-cell/GO:0048821) ([GO:0048821](https://www.ebi.ac.uk/QuickGO/term/GO:0048821)), and [monocyte differentiation](/details-cell/GO:0030224) ([GO:0030224](https://www.ebi.ac.uk/QuickGO/term/GO:0030224)), consistent with its widespread expression in relevant precursor and mature cell types. ## Research Directions Given the foundational role of [MED1](/details-gene/5469) in mediating nuclear receptor signaling, research should focus on its cell-type-specific functions and its potential as a therapeutic target in diseases driven by aberrant transcriptional programs. **Proposed Hypotheses:** 1. Given its high significance in [astrocytes of the cerebral cortex](/details-cell/CL0002605) and its known interactions with steroid and thyroid hormone receptors, [MED1](/details-gene/5469) may be a critical node for integrating hormonal signals that regulate astrocyte metabolic function and neuroinflammatory responses. Dysregulation of [MED1](/details-gene/5469)-dependent transcription in astrocytes could contribute to the pathology of neurodegenerative or neuroinflammatory disorders. 2. The high expression of [MED1](/details-gene/5469) across diverse immune cell types, including B cells, T cells, and monocytes, suggests that it is recruited by distinct lineage-defining transcription factors to orchestrate cell-specific gene expression programs. For example, in monocytes, [MED1](/details-gene/5469) may co-activate PPARγ to promote differentiation towards anti-inflammatory macrophages, while in B cells, it may cooperate with factors like PAX5 or EBF1 to maintain B cell identity. **Experimental Approach:** To test the role of [MED1](/details-gene/5469) in astrocyte function (Hypothesis 1), an astrocyte-specific conditional knockout mouse model could be generated (e.g., using a GFAP-Cre driver). Primary astrocytes could be isolated from knockout and wild-type mice and treated with ligands for relevant nuclear receptors (e.g., estradiol, thyroid hormone T3, or a PPARγ agonist). The resulting transcriptional changes could be profiled using RNA-seq, and key protein-DNA interactions could be mapped via CUT&RUN for MED1 and the relevant nuclear receptor. This would elucidate the specific gene networks controlled by [MED1](/details-gene/5469) in response to hormonal cues in astrocytes. **Therapeutic Potential:** As a core component of the general transcription machinery, global inhibition of [MED1](/details-gene/5469) would likely cause unacceptable toxicity. However, its specific interaction interfaces with nuclear receptors represent attractive therapeutic targets. For hormone-driven cancers, such as estrogen receptor-positive breast cancer or androgen receptor-driven prostate cancer, developing small molecules or peptides that selectively disrupt the interaction between [MED1](/details-gene/5469) and these specific oncodrivers could offer a potent and more targeted therapeutic strategy. Such an approach would aim for inhibition of specific oncogenic transcriptional programs while sparing the global functions of the Mediator complex.

Genular Protein ID: 305185906

Symbol: MED1_HUMAN

Name: Mediator of RNA polymerase II transcription subunit 1

UniProtKB Accession Codes:

Q15648 A2RRQ6 O43810 O75447 Q6P9H7 Q6PK58 Q9HD39

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CORUM 1 CTD 1 ChiTaRS 1 ComplexPortal 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 DrugBank 1 ELM 1 EMBL 8 EMDB 2 Ensembl 2 EvolutionaryTrace 1 ExpressionAtlas 1 GO 83 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyCosmos 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 IDEAL 1 InParanoid 1 IntAct 1 InterPro 2 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 60 PDBsum 60 PRO 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 2 Pumba 1 RNAct 1 Reactome 14 RefSeq 1 SIGNOR 1 SMR 1 STRING 1 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 9444950

Title: Identification of RB18A, a 205 kDa new p53 regulatory protein which shares antigenic and functional properties with p53.

PubMed ID: 9444950

DOI: 10.1038/sj.onc.1201492

PubMed ID: 9653119

Title: The TRAP220 component of a thyroid hormone receptor-associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion.

PubMed ID: 9653119

DOI: 10.1073/pnas.95.14.7939

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 10235267

Title: Composite co-activator ARC mediates chromatin-directed transcriptional activation.

PubMed ID: 10235267

DOI: 10.1038/19789

PubMed ID: 10733574

Title: The DRIP complex and SRC-1/p160 coactivators share similar nuclear receptor binding determinants but constitute functionally distinct complexes.

PubMed ID: 10733574

DOI: 10.1128/mcb.20.8.2718-2726.2000

PubMed ID: 10235266

Title: Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex.

PubMed ID: 10235266

DOI: 10.1038/19783

PubMed ID: 7776974

Title: Two classes of proteins dependent on either the presence or absence of thyroid hormone for interaction with the thyroid hormone receptor.

PubMed ID: 7776974

DOI: 10.1210/mend.9.2.7776974

PubMed ID: 10024883

Title: A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation.

PubMed ID: 10024883

DOI: 10.1016/s1097-2765(00)80178-1

PubMed ID: 10406464

Title: Identification of mouse TRAP100: a transcriptional coregulatory factor for thyroid hormone and vitamin D receptors.

PubMed ID: 10406464

DOI: 10.1210/mend.13.7.0295

PubMed ID: 10478845

Title: Coactivators for the orphan nuclear receptor RORalpha.

PubMed ID: 10478845

DOI: 10.1210/mend.13.9.0343

PubMed ID: 10770935

Title: Functional interactions between the estrogen receptor and DRIP205, a subunit of the heteromeric DRIP coactivator complex.

PubMed ID: 10770935

DOI: 10.1074/jbc.m002013200

PubMed ID: 11303023

Title: Differential recruitment of the mammalian mediator subunit TRAP220 by estrogen receptors ERalpha and ERbeta.

PubMed ID: 11303023

DOI: 10.1074/jbc.m011651200

PubMed ID: 12218053

Title: A coregulatory role for the TRAP-mediator complex in androgen receptor-mediated gene expression.

PubMed ID: 12218053

DOI: 10.1074/jbc.m206061200

PubMed ID: 12037571

Title: Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis.

PubMed ID: 12037571

DOI: 10.1038/417563a

PubMed ID: 11867769

Title: The TRAP/Mediator coactivator complex interacts directly with estrogen receptors alpha and beta through the TRAP220 subunit and directly enhances estrogen receptor function in vitro.

PubMed ID: 11867769

DOI: 10.1073/pnas.261715899

PubMed ID: 12034878

Title: Ordered recruitment of histone acetyltransferases and the TRAP/Mediator complex to thyroid hormone-responsive promoters in vivo.

PubMed ID: 12034878

DOI: 10.1073/pnas.122004799

PubMed ID: 12556447

Title: An extended LXXLL motif sequence determines the nuclear receptor binding specificity of TRAP220.

PubMed ID: 12556447

DOI: 10.1074/jbc.m212950200

PubMed ID: 14636573

Title: Coordination of p300-mediated chromatin remodeling and TRAP/mediator function through coactivator PGC-1alpha.

PubMed ID: 14636573

DOI: 10.1016/s1097-2765(03)00391-5

PubMed ID: 15471764

Title: Vitamin D-interacting protein 205 (DRIP205) coactivation of estrogen receptor alpha (ERalpha) involves multiple domains of both proteins.

PubMed ID: 15471764

DOI: 10.1074/jbc.m409778200

PubMed ID: 15175163

Title: A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.

PubMed ID: 15175163

DOI: 10.1016/j.molcel.2004.05.006

PubMed ID: 15340084

Title: Structural and functional organization of TRAP220, the TRAP/mediator subunit that is targeted by nuclear receptors.

PubMed ID: 15340084

DOI: 10.1128/mcb.24.18.8244-8254.2004

PubMed ID: 15989967

Title: MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription.

PubMed ID: 15989967

DOI: 10.1016/j.molcel.2005.05.015

PubMed ID: 16314496

Title: Activation of TRAP/mediator subunit TRAP220/Med1 is regulated by mitogen-activated protein kinase-dependent phosphorylation.

PubMed ID: 16314496

DOI: 10.1128/mcb.25.24.10695-10710.2005

PubMed ID: 17081983

Title: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.

PubMed ID: 17081983

DOI: 10.1016/j.cell.2006.09.026

PubMed ID: 16574658

Title: Regulation of Aurora-A kinase gene expression via GABP recruitment of TRAP220/MED1.

PubMed ID: 16574658

DOI: 10.1074/jbc.m600163200

PubMed ID: 17000779

Title: Mediator modulates Gli3-dependent Sonic hedgehog signaling.

PubMed ID: 17000779

DOI: 10.1128/mcb.00443-06

PubMed ID: 16964243

Title: A probability-based approach for high-throughput protein phosphorylation analysis and site localization.

PubMed ID: 16964243

DOI: 10.1038/nbt1240

PubMed ID: 17525332

Title: ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.

PubMed ID: 17525332

DOI: 10.1126/science.1140321

PubMed ID: 18691976

Title: Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.

PubMed ID: 18691976

DOI: 10.1016/j.molcel.2008.07.007

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 19413330

Title: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.

PubMed ID: 19413330

DOI: 10.1021/ac9004309

PubMed ID: 19369195

Title: Large-scale proteomics analysis of the human kinome.

PubMed ID: 19369195

DOI: 10.1074/mcp.m800588-mcp200

PubMed ID: 19786558

Title: The basic helix-loop-helix proteins differentiated embryo chondrocyte (DEC) 1 and DEC2 function as corepressors of retinoid X receptors.

PubMed ID: 19786558

DOI: 10.1124/mol.109.057000

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 19608861

Title: Lysine acetylation targets protein complexes and co-regulates major cellular functions.

PubMed ID: 19608861

DOI: 10.1126/science.1175371

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 21406692

Title: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.

PubMed ID: 21406692

DOI: 10.1126/scisignal.2001570

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 24245781

Title: CCAR1/CoCoA pair-mediated recruitment of the Mediator defines a novel pathway for GATA1 function.

PubMed ID: 24245781

DOI: 10.1111/gtc.12104

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 29997176

Title: Affinity switching of the LEDGF/p75 IBD interactome is governed by kinase-dependent phosphorylation.

PubMed ID: 29997176

DOI: 10.1073/pnas.1803909115

Sequence Information:

  • Length: 1581
  • Mass: 168478
  • Checksum: FCE0FE87EF08B887
  • Sequence:
    • MKAQGETEES EKLSKMSSLL ERLHAKFNQN RPWSETIKLV RQVMEKRVVM SSGGHQHLVS 
CLETLQKALK VTSLPAMTDR LESIARQNGL GSHLSASGTE CYITSDMFYV EVQLDPAGQL 
CDVKVAHHGE NPVSCPELVQ QLREKNFDEF SKHLKGLVNL YNLPGDNKLK TKMYLALQSL 
EQDLSKMAIM YWKATNAGPL DKILHGSVGY LTPRSGGHLM NLKYYVSPSD LLDDKTASPI 
ILHENNVSRS LGMNASVTIE GTSAVYKLPI APLIMGSHPV DNKWTPSFSS ITSANSVDLP 
ACFFLKFPQP IPVSRAFVQK LQNCTGIPLF ETQPTYAPLY ELITQFELSK DPDPIPLNHN 
MRFYAALPGQ QHCYFLNKDA PLPDGRSLQG TLVSKITFQH PGRVPLILNL IRHQVAYNTL 
IGSCVKRTIL KEDSPGLLQF EVCPLSESRF SVSFQHPVND SLVCVVMDVQ DSTHVSCKLY 
KGLSDALICT DDFIAKVVQR CMSIPVTMRA IRRKAETIQA DTPALSLIAE TVEDMVKKNL 
PPASSPGYGM TTGNNPMSGT TTPTNTFPGG PITTLFNMSM SIKDRHESVG HGEDFSKVSQ 
NPILTSLLQI TGNGGSTIGS SPTPPHHTPP PVSSMAGNTK NHPMLMNLLK DNPAQDFSTL 
YGSSPLERQN SSSGSPRMEI CSGSNKTKKK KSSRLPPEKP KHQTEDDFQR ELFSMDVDSQ 
NPIFDVNMTA DTLDTPHITP APSQCSTPPT TYPQPVPHPQ PSIQRMVRLS SSDSIGPDVT 
DILSDIAEEA SKLPSTSDDC PAIGTPLRDS SSSGHSQSTL FDSDVFQTNN NENPYTDPAD 
LIADAAGSPS SDSPTNHFFH DGVDFNPDLL NSQSQSGFGE EYFDESSQSG DNDDFKGFAS 
QALNTLGVPM LGGDNGETKF KGNNQADTVD FSIISVAGKA LAPADLMEHH SGSQGPLLTT 
GDLGKEKTQK RVKEGNGTSN STLSGPGLDS KPGKRSRTPS NDGKSKDKPP KRKKADTEGK 
SPSHSSSNRP FTPPTSTGGS KSPGSAGRSQ TPPGVATPPI PKITIQIPKG TVMVGKPSSH 
SQYTSSGSVS SSGSKSHHSH SSSSSSSAST SGKMKSSKSE GSSSSKLSSS MYSSQGSSGS 
SQSKNSSQSG GKPGSSPITK HGLSSGSSST KMKPQGKPSS LMNPSLSKPN ISPSHSRPPG 
GSDKLASPMK PVPGTPPSSK AKSPISSGSG GSHMSGTSSS SGMKSSSGLG SSGSLSQKTP 
PSSNSCTASS SSFSSSGSSM SSSQNQHGSS KGKSPSRNKK PSLTAVIDKL KHGVVTSGPG 
GEDPLDGQMG VSTNSSSHPM SSKHNMSGGE FQGKREKSDK DKSKVSTSGS SVDSSKKTSE 
SKNVGSTGVA KIIISKHDGG SPSIKAKVTL QKPGESSGEG LRPQMASSKN YGSPLISGST 
PKHERGSPSH SKSPAYTPQN LDSESESGSS IAEKSYQNSP SSDDGIRPLP EYSTEKHKKH 
KKEKKKVKDK DRDRDRDKDR DKKKSHSIKP ESWSKSPISS DQSLSMTSNT ILSADRPSRL 
SPDFMIGEED DDLMDVALIG N