Details for: TOP2A

Gene ID: 7153

Symbol: TOP2A

Ensembl ID: ENSG00000131747

Description: DNA topoisomerase II alpha

Associated with

Cells (max top 100)

(Cell Significance Index and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: hematopoietic oligopotent progenitor cell (CL0002032)
    Fold Change: 60.2815
    Cell Significance Index: -15.2900
  • Cell Name: embryonic stem cell (CL0002322)
    Fold Change: 46.9841
    Cell Significance Index: -19.3600
  • Cell Name: mucosal type mast cell (CL0000485)
    Fold Change: 32.6640
    Cell Significance Index: -13.2700
  • Cell Name: basophilic erythroblast (CL0000549)
    Fold Change: 19.7626
    Cell Significance Index: 9.0000
  • Cell Name: ciliated cell of the bronchus (CL0002332)
    Fold Change: 13.9409
    Cell Significance Index: -13.3100
  • Cell Name: orthochromatic erythroblast (CL0000552)
    Fold Change: 11.7198
    Cell Significance Index: -14.4500
  • Cell Name: stromal cell of bone marrow (CL0010001)
    Fold Change: 4.0268
    Cell Significance Index: -15.8900
  • Cell Name: sensory neuron (CL0000101)
    Fold Change: 3.7086
    Cell Significance Index: 21.0800
  • Cell Name: germ cell (CL0000586)
    Fold Change: 2.6759
    Cell Significance Index: 20.2100
  • Cell Name: skeletal muscle myoblast (CL0000515)
    Fold Change: 2.1984
    Cell Significance Index: 23.9000
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 1.9451
    Cell Significance Index: 386.0100
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 1.8766
    Cell Significance Index: 305.2100
  • Cell Name: retinal progenitor cell (CL0002672)
    Fold Change: 1.7482
    Cell Significance Index: 98.1000
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 1.5685
    Cell Significance Index: 184.9700
  • Cell Name: epidermal Langerhans cell (CL0002457)
    Fold Change: 1.5648
    Cell Significance Index: -3.4200
  • Cell Name: colon goblet cell (CL0009039)
    Fold Change: 1.5187
    Cell Significance Index: 150.2400
  • Cell Name: enteroendocrine cell of colon (CL0009042)
    Fold Change: 1.4659
    Cell Significance Index: 278.9800
  • Cell Name: oral mucosa squamous cell (CL1001576)
    Fold Change: 1.4535
    Cell Significance Index: 12.4900
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 1.4306
    Cell Significance Index: 155.6100
  • Cell Name: mesonephric nephron tubule epithelial cell (CL1000022)
    Fold Change: 1.3505
    Cell Significance Index: 46.9300
  • Cell Name: epithelial cell of stomach (CL0002178)
    Fold Change: 1.1769
    Cell Significance Index: 137.1500
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 1.0822
    Cell Significance Index: 977.1600
  • Cell Name: fibroblast of dermis (CL0002551)
    Fold Change: 1.0248
    Cell Significance Index: 21.4500
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: 0.9956
    Cell Significance Index: 27.8200
  • Cell Name: tuft cell of small intestine (CL0009080)
    Fold Change: 0.8922
    Cell Significance Index: 9.0000
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: 0.8667
    Cell Significance Index: 27.7600
  • Cell Name: ovarian surface epithelial cell (CL2000064)
    Fold Change: 0.7042
    Cell Significance Index: 3.4300
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 0.6356
    Cell Significance Index: 43.9600
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 0.6349
    Cell Significance Index: 346.7600
  • Cell Name: salivary gland cell (CL0009005)
    Fold Change: 0.6185
    Cell Significance Index: 7.6800
  • Cell Name: pro-T cell (CL0000827)
    Fold Change: 0.6134
    Cell Significance Index: 15.6700
  • Cell Name: enteroendocrine cell of small intestine (CL0009006)
    Fold Change: 0.4817
    Cell Significance Index: 12.0400
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.4453
    Cell Significance Index: 12.8300
  • Cell Name: neuroblast (sensu Nematoda and Protostomia) (CL0000338)
    Fold Change: 0.4422
    Cell Significance Index: 4.0200
  • Cell Name: fetal cardiomyocyte (CL0002495)
    Fold Change: 0.3681
    Cell Significance Index: 1.0600
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: 0.3430
    Cell Significance Index: 17.8200
  • Cell Name: cortical cell of adrenal gland (CL0002097)
    Fold Change: 0.3394
    Cell Significance Index: 9.1000
  • Cell Name: late promyelocyte (CL0002151)
    Fold Change: 0.3000
    Cell Significance Index: 1.9900
  • Cell Name: glioblast (CL0000030)
    Fold Change: 0.2436
    Cell Significance Index: 1.5300
  • Cell Name: epidermal cell (CL0000362)
    Fold Change: 0.1576
    Cell Significance Index: 0.3600
  • Cell Name: hair follicular keratinocyte (CL2000092)
    Fold Change: 0.1532
    Cell Significance Index: 67.7500
  • Cell Name: Sertoli cell (CL0000216)
    Fold Change: 0.1526
    Cell Significance Index: 2.1400
  • Cell Name: intestinal crypt stem cell of small intestine (CL0009017)
    Fold Change: 0.1242
    Cell Significance Index: 2.6500
  • Cell Name: paneth cell of epithelium of small intestine (CL1000343)
    Fold Change: 0.0974
    Cell Significance Index: 2.1100
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: 0.0790
    Cell Significance Index: 2.1100
  • Cell Name: enterocyte of epithelium of large intestine (CL0002071)
    Fold Change: 0.0741
    Cell Significance Index: 3.3600
  • Cell Name: intermediate cell of urothelium (CL4030055)
    Fold Change: 0.0631
    Cell Significance Index: 11.3800
  • Cell Name: basal cell of urothelium (CL1000486)
    Fold Change: 0.0499
    Cell Significance Index: 6.1400
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.0478
    Cell Significance Index: 1.6800
  • Cell Name: pancreatic PP cell (CL0002275)
    Fold Change: 0.0471
    Cell Significance Index: 29.4400
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: 0.0367
    Cell Significance Index: 0.6200
  • Cell Name: GABAergic interneuron (CL0011005)
    Fold Change: 0.0258
    Cell Significance Index: 17.8400
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: -0.0052
    Cell Significance Index: -3.8800
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: -0.0069
    Cell Significance Index: -13.0100
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: -0.0133
    Cell Significance Index: -24.5100
  • Cell Name: pulmonary alveolar epithelial cell (CL0000322)
    Fold Change: -0.0150
    Cell Significance Index: -11.3800
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: -0.0153
    Cell Significance Index: -8.6100
  • Cell Name: lens epithelial cell (CL0002224)
    Fold Change: -0.0157
    Cell Significance Index: -24.2200
  • Cell Name: secondary lens fiber (CL0002225)
    Fold Change: -0.0183
    Cell Significance Index: -24.8700
  • Cell Name: stromal cell of ovary (CL0002132)
    Fold Change: -0.0200
    Cell Significance Index: -2.7400
  • Cell Name: intestinal epithelial cell (CL0002563)
    Fold Change: -0.0217
    Cell Significance Index: -0.2300
  • Cell Name: non-pigmented ciliary epithelial cell (CL0002304)
    Fold Change: -0.0224
    Cell Significance Index: -14.2500
  • Cell Name: colon endothelial cell (CL1001572)
    Fold Change: -0.0262
    Cell Significance Index: -0.0200
  • Cell Name: pancreatic D cell (CL0000173)
    Fold Change: -0.0297
    Cell Significance Index: -6.2500
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: -0.0356
    Cell Significance Index: -16.1800
  • Cell Name: obsolete caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: -0.0377
    Cell Significance Index: -13.5400
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: -0.0480
    Cell Significance Index: -8.1900
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: -0.0484
    Cell Significance Index: -4.9500
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: -0.0486
    Cell Significance Index: -13.9900
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: -0.0628
    Cell Significance Index: -12.6100
  • Cell Name: gut absorptive cell (CL0000677)
    Fold Change: -0.0633
    Cell Significance Index: -3.8000
  • Cell Name: neutrophil progenitor cell (CL0000834)
    Fold Change: -0.0677
    Cell Significance Index: -1.8100
  • Cell Name: forebrain radial glial cell (CL0013000)
    Fold Change: -0.0853
    Cell Significance Index: -0.6200
  • Cell Name: fallopian tube secretory epithelial cell (CL4030006)
    Fold Change: -0.0885
    Cell Significance Index: -1.3700
  • Cell Name: pigmented ciliary epithelial cell (CL0002303)
    Fold Change: -0.0935
    Cell Significance Index: -13.6000
  • Cell Name: forebrain neuroblast (CL1000042)
    Fold Change: -0.0955
    Cell Significance Index: -5.8700
  • Cell Name: lung endothelial cell (CL1001567)
    Fold Change: -0.0968
    Cell Significance Index: -5.0400
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: -0.1004
    Cell Significance Index: -6.4800
  • Cell Name: granulosa cell (CL0000501)
    Fold Change: -0.1129
    Cell Significance Index: -2.9700
  • Cell Name: pancreatic endocrine cell (CL0008024)
    Fold Change: -0.1133
    Cell Significance Index: -12.9300
  • Cell Name: lactocyte (CL0002325)
    Fold Change: -0.1150
    Cell Significance Index: -14.8600
  • Cell Name: pancreatic ductal cell (CL0002079)
    Fold Change: -0.1178
    Cell Significance Index: -13.5000
  • Cell Name: odontoblast (CL0000060)
    Fold Change: -0.1334
    Cell Significance Index: -17.1000
  • Cell Name: radial glial cell (CL0000681)
    Fold Change: -0.1407
    Cell Significance Index: -0.8400
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: -0.1426
    Cell Significance Index: -8.7400
  • Cell Name: hepatoblast (CL0005026)
    Fold Change: -0.1492
    Cell Significance Index: -2.5100
  • Cell Name: smooth muscle cell of sphincter of pupil (CL0002243)
    Fold Change: -0.1493
    Cell Significance Index: -15.5500
  • Cell Name: transit amplifying cell of small intestine (CL0009012)
    Fold Change: -0.1497
    Cell Significance Index: -3.1100
  • Cell Name: CD4-positive, alpha-beta memory T cell, CD45RO-positive (CL0001204)
    Fold Change: -0.1590
    Cell Significance Index: -4.6700
  • Cell Name: sebum secreting cell (CL0000317)
    Fold Change: -0.1722
    Cell Significance Index: -12.1800
  • Cell Name: progenitor cell of mammary luminal epithelium (CL0009116)
    Fold Change: -0.2234
    Cell Significance Index: -16.6500
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: -0.2317
    Cell Significance Index: -17.7800
  • Cell Name: kidney epithelial cell (CL0002518)
    Fold Change: -0.2349
    Cell Significance Index: -6.9200
  • Cell Name: hippocampal granule cell (CL0001033)
    Fold Change: -0.2451
    Cell Significance Index: -16.4800
  • Cell Name: luminal adaptive secretory precursor cell of mammary gland (CL4033057)
    Fold Change: -0.2509
    Cell Significance Index: -11.7900
  • Cell Name: eye photoreceptor cell (CL0000287)
    Fold Change: -0.2532
    Cell Significance Index: -15.9600
  • Cell Name: medial ganglionic eminence derived interneuron (CL4023063)
    Fold Change: -0.2674
    Cell Significance Index: -3.8300
  • Cell Name: BEST4+ enteroycte (CL4030026)
    Fold Change: -0.2681
    Cell Significance Index: -4.0400
  • Cell Name: glycinergic neuron (CL1001509)
    Fold Change: -0.3270
    Cell Significance Index: -17.1700
  • Cell Name: ependymal cell (CL0000065)
    Fold Change: -0.3581
    Cell Significance Index: -4.2200

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics:** 1. **DNA topoisomerase activity**: TOP2A is a type II topoisomerase that catalyzes the transient unwinding and re-ligation of DNA, enabling the covalent binding of proteins to DNA and facilitating the regulation of chromosomal processes. 2. **Cell cycle regulation**: TOP2A is involved in the control of cell cycle progression, particularly during the G1/S transition and mitosis, where it regulates DNA replication and segregation. 3. **Apoptosis regulation**: TOP2A has been shown to modulate apoptosis by regulating the expression of pro-apoptotic and anti-apoptotic genes, as well as interacting with key apoptosis regulators, such as p53 and Bcl-2. 4. **Chromatin binding**: TOP2A exhibits a high affinity for chromatin, allowing it to interact with transcription factors and other chromatin-associated proteins, influencing gene expression and chromatin structure. 5. **Sumoylation and ubiquitination**: TOP2A is a substrate for sumoylation and ubiquitination, which regulate its activity, localization, and interaction with other proteins. **Pathways and Functions:** 1. **Apoptotic chromosome condensation**: TOP2A regulates the condensation of apoptotic chromosomes, ensuring proper segregation of DNA during mitosis. 2. **DNA damage response**: TOP2A participates in the DNA damage response by regulating the expression of genes involved in DNA repair and apoptosis. 3. **Cell cycle regulation**: TOP2A modulates cell cycle progression by regulating the expression of cyclin-dependent kinases and the activity of the cyclin-dependent kinase inhibitor, p21. 4. **Transcriptional regulation**: TOP2A interacts with transcription factors, such as NF-κB and p53, to regulate the expression of genes involved in cell growth, differentiation, and survival. 5. **Chromatin remodeling**: TOP2A contributes to chromatin remodeling by regulating the activity of chromatin-associated proteins, such as histone deacetylases and chromatin-modifying enzymes. **Clinical Significance:** 1. **Cancer therapy**: TOP2A is a target for cancer therapy, as its inhibition has been shown to induce apoptosis in various cancer cell lines, including those with mutated p53. 2. **Neurodegenerative diseases**: TOP2A has been implicated in neurodegenerative diseases, such as Alzheimer's and Parkinson's, where it may contribute to the regulation of chromatin structure and gene expression. 3. **Immune system disorders**: TOP2A is expressed in immune cells, and its dysregulation has been linked to immune system disorders, such as rheumatoid arthritis and multiple sclerosis. 4. **Developmental disorders**: TOP2A is essential for embryonic development, and its dysregulation has been implicated in developmental disorders, such as neural tube defects and congenital heart disease. In conclusion, DNA topoisomerase II alpha is a multifunctional protein that plays a critical role in maintaining genomic stability, regulating cell cycle progression, and modulating apoptosis. Its dysregulation has been implicated in various diseases, highlighting the importance of further research into the mechanisms of TOP2A and its potential as a therapeutic target.

Genular Protein ID: 2528300018

Symbol: TOP2A_HUMAN

Name: DNA topoisomerase 2-alpha

UniProtKB Accession Codes:

P11388 B2RTS1 Q71UN1 Q71UQ5 Q9HB24 Q9HB25 Q9HB26 Q9UP44 Q9UQP9

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 BRENDA 2 Bgee 1 BindingDB 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 4 CORUM 1 CPTAC 1 CTD 1 CarbonylDB 1 ChEBI 12 ChEMBL 1 ChiTaRS 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 DrugBank 37 DrugCentral 1 EC 1 EMBL 37 EMDB 5 Ensembl 1 EvolutionaryTrace 1 ExpressionAtlas 1 GO 38 Gene3D 7 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyGen 1 GuidetoPHARMACOLOGY 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 18 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MalaCards 1 MassIVE 1 MetOSite 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 10 PDBsum 10 PIR 1 PRINTS 2 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 6 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 4 Pumba 1 RNAct 1 Reactome 2 RefSeq 1 SIGNOR 1 SMART 2 SMR 1 STRING 1 SUPFAM 3 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 2845399

Title: Cloning and sequencing of cDNA encoding human DNA topoisomerase II and localization of the gene to chromosome region 17q21-22.

PubMed ID: 2845399

DOI: 10.1073/pnas.85.19.7177

PubMed ID: 8393377

Title: Use of yeast in the study of anticancer drugs targeting DNA topoisomerases: expression of a functional recombinant human DNA topoisomerase II alpha in yeast.

PubMed ID: 8393377

PubMed ID: 9795238

Title: Structural organization of the human TOP2A and TOP2B genes.

PubMed ID: 9795238

DOI: 10.1016/s0378-1119(98)00468-5

PubMed ID: 10095062

Title: Molecular cloning and characterization of the human topoisomerase IIalpha and IIbeta genes: evidence for isoform evolution through gene duplication.

PubMed ID: 10095062

DOI: 10.1016/s0167-4781(99)00020-2

PubMed ID: 16625196

Title: DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.

PubMed ID: 16625196

DOI: 10.1038/nature04689

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 8299728

Title: Discrete localization of different DNA topoisomerases in HeLa and K562 cell nuclei and subnuclear fractions.

PubMed ID: 8299728

DOI: 10.1006/excr.1994.1046

PubMed ID: 9155056

Title: The distribution and expression of the two isoforms of DNA topoisomerase II in normal and neoplastic human tissues.

PubMed ID: 9155056

DOI: 10.1038/bjc.1997.227

PubMed ID: 10942766

Title: Mitotic phosphorylation of DNA topoisomerase II alpha by protein kinase CK2 creates the MPM-2 phosphoepitope on Ser-1469.

PubMed ID: 10942766

DOI: 10.1074/jbc.m005179200

PubMed ID: 12821127

Title: Identification of functional nuclear export sequences in human topoisomerase IIalpha and beta.

PubMed ID: 12821127

DOI: 10.1016/s0006-291x(03)01077-5

PubMed ID: 12711669

Title: RNA helicase A interacts with dsDNA and topoisomerase IIalpha.

PubMed ID: 12711669

DOI: 10.1093/nar/gkg328

PubMed ID: 15126503

Title: Interaction between glucose-regulated destruction domain of DNA topoisomerase IIalpha and MPN domain of Jab1/CSN5.

PubMed ID: 15126503

DOI: 10.1074/jbc.m401411200

PubMed ID: 17081983

Title: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.

PubMed ID: 17081983

DOI: 10.1016/j.cell.2006.09.026

PubMed ID: 16964243

Title: A probability-based approach for high-throughput protein phosphorylation analysis and site localization.

PubMed ID: 16964243

DOI: 10.1038/nbt1240

PubMed ID: 17924679

Title: Improved titanium dioxide enrichment of phosphopeptides from HeLa cells and high confident phosphopeptide identification by cross-validation of MS/MS and MS/MS/MS spectra.

PubMed ID: 17924679

DOI: 10.1021/pr070152u

PubMed ID: 17567603

Title: Nuclear interactions of topoisomerase II alpha and beta with phospholipid scramblase 1.

PubMed ID: 17567603

DOI: 10.1093/nar/gkm434

PubMed ID: 18062778

Title: Plk3 phosphorylates topoisomerase IIalpha at Thr(1342), a site that is not recognized by Plk1.

PubMed ID: 18062778

DOI: 10.1042/bj20071394

PubMed ID: 18220336

Title: Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.

PubMed ID: 18220336

DOI: 10.1021/pr0705441

PubMed ID: 18691976

Title: Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.

PubMed ID: 18691976

DOI: 10.1016/j.molcel.2008.07.007

PubMed ID: 18790802

Title: The SET and transposase domain protein Metnase enhances chromosome decatenation: regulation by automethylation.

PubMed ID: 18790802

DOI: 10.1093/nar/gkn560

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 19413330

Title: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.

PubMed ID: 19413330

DOI: 10.1021/ac9004309

PubMed ID: 19222228

Title: Use of divalent metal ions in the DNA cleavage reaction of human type II topoisomerases.

PubMed ID: 19222228

DOI: 10.1021/bi8023256

PubMed ID: 19697956

Title: Metal ion interactions in the DNA cleavage/ligation active site of human topoisomerase IIalpha.

PubMed ID: 19697956

DOI: 10.1021/bi900875c

PubMed ID: 19043076

Title: Casein kinase I delta/epsilon phosphorylates topoisomerase IIalpha at serine-1106 and modulates DNA cleavage activity.

PubMed ID: 19043076

DOI: 10.1093/nar/gkn934

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 20457750

Title: Metnase promotes restart and repair of stalled and collapsed replication forks.

PubMed ID: 20457750

DOI: 10.1093/nar/gkq339

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 21406692

Title: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.

PubMed ID: 21406692

DOI: 10.1126/scisignal.2001570

PubMed ID: 23213405

Title: Taperin (c9orf75), a mutated gene in nonsyndromic deafness, encodes a vertebrate specific, nuclear localized protein phosphatase one alpha (PP1alpha) docking protein.

PubMed ID: 23213405

DOI: 10.1242/bio.2011049

PubMed ID: 23022727

Title: Structure of a topoisomerase II-DNA-nucleotide complex reveals a new control mechanism for ATPase activity.

PubMed ID: 23022727

DOI: 10.1038/nsmb.2388

PubMed ID: 22013166

Title: RECQL5 cooperates with Topoisomerase II alpha in DNA decatenation and cell cycle progression.

PubMed ID: 22013166

DOI: 10.1093/nar/gkr844

PubMed ID: 22814378

Title: N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

PubMed ID: 22814378

DOI: 10.1073/pnas.1210303109

PubMed ID: 22323612

Title: DNA cleavage and opening reactions of human topoisomerase IIalpha are regulated via Mg2+-mediated dynamic bending of gate-DNA.

PubMed ID: 22323612

DOI: 10.1073/pnas.1115704109

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 23652018

Title: GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy.

PubMed ID: 23652018

DOI: 10.1038/ncomms2823

PubMed ID: 25218447

Title: Uncovering global SUMOylation signaling networks in a site-specific manner.

PubMed ID: 25218447

DOI: 10.1038/nsmb.2890

PubMed ID: 25114211

Title: Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.

PubMed ID: 25114211

DOI: 10.1073/pnas.1413825111

PubMed ID: 25772364

Title: SUMO-2 orchestrates chromatin modifiers in response to DNA damage.

PubMed ID: 25772364

DOI: 10.1016/j.celrep.2015.02.033

PubMed ID: 25755297

Title: System-wide analysis of SUMOylation dynamics in response to replication stress reveals novel small ubiquitin-like modified target proteins and acceptor lysines relevant for genome stability.

PubMed ID: 25755297

DOI: 10.1074/mcp.o114.044792

PubMed ID: 26030138

Title: Identification of Novel Proteins Co-Purifying with Cockayne Syndrome Group B (CSB) Reveals Potential Roles for CSB in RNA Metabolism and Chromatin Dynamics.

PubMed ID: 26030138

DOI: 10.1371/journal.pone.0128558

PubMed ID: 28112733

Title: Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.

PubMed ID: 28112733

DOI: 10.1038/nsmb.3366

PubMed ID: 34543352

Title: The Epstein-Barr virus deubiquitinating enzyme BPLF1 regulates the activity of topoisomerase II during productive infection.

PubMed ID: 34543352

DOI: 10.1371/journal.ppat.1009954

PubMed ID: 36271492

Title: The human RNA polymerase I structure reveals an HMG-like docking domain specific to metazoans.

PubMed ID: 36271492

DOI: 10.26508/lsa.202201568

PubMed ID: 16100112

Title: Nucleotide-dependent domain movement in the ATPase domain of a human type IIA DNA topoisomerase.

PubMed ID: 16100112

DOI: 10.1074/jbc.m506520200

PubMed ID: 22841979

Title: The structure of DNA-bound human topoisomerase II alpha: conformational mechanisms for coordinating inter-subunit interactions with DNA cleavage.

PubMed ID: 22841979

DOI: 10.1016/j.jmb.2012.07.014

PubMed ID: 25202966

Title: Structure of the N-terminal Gyrase B fragment in complex with ADPPi reveals rigid-body motion induced by ATP hydrolysis.

PubMed ID: 25202966

DOI: 10.1371/journal.pone.0107289

PubMed ID: 1651812

Title: Identification of a point mutation in the topoisomerase II gene from a human leukemia cell line containing an amsacrine-resistant form of topoisomerase II.

PubMed ID: 1651812

PubMed ID: 1652758

Title: Expression of a mutant DNA topoisomerase II in CCRF-CEM human leukemic cells selected for resistance to teniposide.

PubMed ID: 1652758

DOI: 10.1073/pnas.88.17.7654

Sequence Information:

  • Length: 1531
  • Mass: 174385
  • Checksum: 3DF40BC9E84789DC
  • Sequence:
    • MEVSPLQPVN ENMQVNKIKK NEDAKKRLSV ERIYQKKTQL EHILLRPDTY IGSVELVTQQ 
MWVYDEDVGI NYREVTFVPG LYKIFDEILV NAADNKQRDP KMSCIRVTID PENNLISIWN 
NGKGIPVVEH KVEKMYVPAL IFGQLLTSSN YDDDEKKVTG GRNGYGAKLC NIFSTKFTVE 
TASREYKKMF KQTWMDNMGR AGEMELKPFN GEDYTCITFQ PDLSKFKMQS LDKDIVALMV 
RRAYDIAGST KDVKVFLNGN KLPVKGFRSY VDMYLKDKLD ETGNSLKVIH EQVNHRWEVC 
LTMSEKGFQQ ISFVNSIATS KGGRHVDYVA DQIVTKLVDV VKKKNKGGVA VKAHQVKNHM 
WIFVNALIEN PTFDSQTKEN MTLQPKSFGS TCQLSEKFIK AAIGCGIVES ILNWVKFKAQ 
VQLNKKCSAV KHNRIKGIPK LDDANDAGGR NSTECTLILT EGDSAKTLAV SGLGVVGRDK 
YGVFPLRGKI LNVREASHKQ IMENAEINNI IKIVGLQYKK NYEDEDSLKT LRYGKIMIMT 
DQDQDGSHIK GLLINFIHHN WPSLLRHRFL EEFITPIVKV SKNKQEMAFY SLPEFEEWKS 
STPNHKKWKV KYYKGLGTST SKEAKEYFAD MKRHRIQFKY SGPEDDAAIS LAFSKKQIDD 
RKEWLTNFME DRRQRKLLGL PEDYLYGQTT TYLTYNDFIN KELILFSNSD NERSIPSMVD 
GLKPGQRKVL FTCFKRNDKR EVKVAQLAGS VAEMSSYHHG EMSLMMTIIN LAQNFVGSNN 
LNLLQPIGQF GTRLHGGKDS ASPRYIFTML SSLARLLFPP KDDHTLKFLY DDNQRVEPEW 
YIPIIPMVLI NGAEGIGTGW SCKIPNFDVR EIVNNIRRLM DGEEPLPMLP SYKNFKGTIE 
ELAPNQYVIS GEVAILNSTT IEISELPVRT WTQTYKEQVL EPMLNGTEKT PPLITDYREY 
HTDTTVKFVV KMTEEKLAEA ERVGLHKVFK LQTSLTCNSM VLFDHVGCLK KYDTVLDILR 
DFFELRLKYY GLRKEWLLGM LGAESAKLNN QARFILEKID GKIIIENKPK KELIKVLIQR 
GYDSDPVKAW KEAQQKVPDE EENEESDNEK ETEKSDSVTD SGPTFNYLLD MPLWYLTKEK 
KDELCRLRNE KEQELDTLKR KSPSDLWKED LATFIEELEA VEAKEKQDEQ VGLPGKGGKA 
KGKKTQMAEV LPSPRGQRVI PRITIEMKAE AEKKNKKKIK NENTEGSPQE DGVELEGLKQ 
RLEKKQKREP GTKTKKQTTL AFKPIKKGKK RNPWSDSESD RSSDESNFDV PPRETEPRRA 
ATKTKFTMDL DSDEDFSDFD EKTDDEDFVP SDASPPKTKT SPKLSNKELK PQKSVVSDLE 
ADDVKGSVPL SSSPPATHFP DETEITNPVP KKNVTVKKTA AKSQSSTSTT GAKKRAAPKG 
TKRDPALNSG VSQKPDPAKT KNRRKRKPST SDDSDSNFEK IVSKAVTSKK SKGESDDFHM 
DFDSAVAPRA KSVRAKKPIK YLEESDEDDL F

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.