Details for: DLAT

Gene ID: 1737

Symbol: DLAT

Ensembl ID: ENSG00000150768

Description: dihydrolipoamide S-acetyltransferase

Associated with

Cells (max top 100)

(Cell Significance Index and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: polychromatophilic erythroblast (CL0000550)
    Fold Change: 113.6968
    Cell Significance Index: -17.6900
  • Cell Name: hematopoietic oligopotent progenitor cell (CL0002032)
    Fold Change: 74.8098
    Cell Significance Index: -18.9800
  • Cell Name: mucosal type mast cell (CL0000485)
    Fold Change: 45.2915
    Cell Significance Index: -18.4000
  • Cell Name: ileal goblet cell (CL1000326)
    Fold Change: 33.6949
    Cell Significance Index: -22.6100
  • Cell Name: ciliated cell of the bronchus (CL0002332)
    Fold Change: 19.3141
    Cell Significance Index: -18.4400
  • Cell Name: orthochromatic erythroblast (CL0000552)
    Fold Change: 16.3307
    Cell Significance Index: -20.1400
  • Cell Name: CD8-alpha-beta-positive, alpha-beta intraepithelial T cell (CL0000796)
    Fold Change: 7.5555
    Cell Significance Index: -20.2400
  • Cell Name: stromal cell of bone marrow (CL0010001)
    Fold Change: 5.1900
    Cell Significance Index: -20.4800
  • Cell Name: CD8-positive, alpha-beta regulatory T cell (CL0000795)
    Fold Change: 4.6134
    Cell Significance Index: -14.1700
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 1.7921
    Cell Significance Index: 123.9300
  • Cell Name: enteroendocrine cell of colon (CL0009042)
    Fold Change: 1.7170
    Cell Significance Index: 326.7500
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 1.1465
    Cell Significance Index: 1035.2100
  • Cell Name: gut absorptive cell (CL0000677)
    Fold Change: 1.1309
    Cell Significance Index: 67.9000
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 1.0462
    Cell Significance Index: 113.8000
  • Cell Name: conjunctival epithelial cell (CL1000432)
    Fold Change: 0.9843
    Cell Significance Index: 13.4300
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.8707
    Cell Significance Index: 141.6200
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: 0.7722
    Cell Significance Index: 59.2600
  • Cell Name: colon goblet cell (CL0009039)
    Fold Change: 0.6732
    Cell Significance Index: 66.5900
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 0.6298
    Cell Significance Index: 126.3300
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.4727
    Cell Significance Index: 13.6200
  • Cell Name: eye photoreceptor cell (CL0000287)
    Fold Change: 0.4482
    Cell Significance Index: 28.2500
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.3845
    Cell Significance Index: 76.3200
  • Cell Name: obsolete caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: 0.3453
    Cell Significance Index: 123.8500
  • Cell Name: GABAergic interneuron (CL0011005)
    Fold Change: 0.3443
    Cell Significance Index: 238.1200
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: 0.3355
    Cell Significance Index: 17.4300
  • Cell Name: cardiac muscle cell (CL0000746)
    Fold Change: 0.3177
    Cell Significance Index: 4.6900
  • Cell Name: intermediate cell of urothelium (CL4030055)
    Fold Change: 0.3030
    Cell Significance Index: 54.6300
  • Cell Name: paneth cell of epithelium of small intestine (CL1000343)
    Fold Change: 0.2885
    Cell Significance Index: 6.2500
  • Cell Name: basal cell of urothelium (CL1000486)
    Fold Change: 0.2624
    Cell Significance Index: 32.2700
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 0.2611
    Cell Significance Index: 142.5900
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: 0.1898
    Cell Significance Index: 5.3100
  • Cell Name: hair follicular keratinocyte (CL2000092)
    Fold Change: 0.1816
    Cell Significance Index: 80.3100
  • Cell Name: acinar cell of salivary gland (CL0002623)
    Fold Change: 0.1456
    Cell Significance Index: 6.7900
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 0.1403
    Cell Significance Index: 16.5400
  • Cell Name: stromal cell of ovary (CL0002132)
    Fold Change: 0.1180
    Cell Significance Index: 16.2100
  • Cell Name: enterocyte of epithelium of large intestine (CL0002071)
    Fold Change: 0.1055
    Cell Significance Index: 4.7800
  • Cell Name: retinal progenitor cell (CL0002672)
    Fold Change: 0.0855
    Cell Significance Index: 4.8000
  • Cell Name: indirect pathway medium spiny neuron (CL4023029)
    Fold Change: 0.0784
    Cell Significance Index: 3.4700
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: 0.0777
    Cell Significance Index: 2.4900
  • Cell Name: enteroendocrine cell of small intestine (CL0009006)
    Fold Change: 0.0702
    Cell Significance Index: 1.7600
  • Cell Name: sebum secreting cell (CL0000317)
    Fold Change: 0.0698
    Cell Significance Index: 4.9400
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: 0.0595
    Cell Significance Index: 10.1600
  • Cell Name: direct pathway medium spiny neuron (CL4023026)
    Fold Change: 0.0449
    Cell Significance Index: 1.7000
  • Cell Name: glycinergic neuron (CL1001509)
    Fold Change: 0.0429
    Cell Significance Index: 2.2500
  • Cell Name: odontoblast (CL0000060)
    Fold Change: 0.0234
    Cell Significance Index: 3.0000
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.0228
    Cell Significance Index: 0.8000
  • Cell Name: hippocampal granule cell (CL0001033)
    Fold Change: 0.0193
    Cell Significance Index: 1.3000
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: 0.0143
    Cell Significance Index: 0.2400
  • Cell Name: non-pigmented ciliary epithelial cell (CL0002304)
    Fold Change: 0.0127
    Cell Significance Index: 8.0900
  • Cell Name: kidney loop of Henle cortical thick ascending limb epithelial cell (CL1001109)
    Fold Change: 0.0117
    Cell Significance Index: 8.5600
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: 0.0054
    Cell Significance Index: 10.0800
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: 0.0019
    Cell Significance Index: 0.8700
  • Cell Name: lens epithelial cell (CL0002224)
    Fold Change: 0.0018
    Cell Significance Index: 2.7800
  • Cell Name: transit amplifying cell of small intestine (CL0009012)
    Fold Change: 0.0017
    Cell Significance Index: 0.0400
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: -0.0005
    Cell Significance Index: -0.9600
  • Cell Name: proerythroblast (CL0000547)
    Fold Change: -0.0049
    Cell Significance Index: -0.0700
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: -0.0052
    Cell Significance Index: -3.8700
  • Cell Name: pancreatic PP cell (CL0002275)
    Fold Change: -0.0075
    Cell Significance Index: -4.6600
  • Cell Name: subcutaneous adipocyte (CL0002521)
    Fold Change: -0.0092
    Cell Significance Index: -0.0400
  • Cell Name: pulmonary alveolar epithelial cell (CL0000322)
    Fold Change: -0.0096
    Cell Significance Index: -7.2400
  • Cell Name: secondary lens fiber (CL0002225)
    Fold Change: -0.0107
    Cell Significance Index: -14.5100
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: -0.0112
    Cell Significance Index: -0.3000
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: -0.0216
    Cell Significance Index: -12.2000
  • Cell Name: lactocyte (CL0002325)
    Fold Change: -0.0237
    Cell Significance Index: -3.0600
  • Cell Name: basal cell of prostate epithelium (CL0002341)
    Fold Change: -0.0252
    Cell Significance Index: -0.6900
  • Cell Name: pancreatic ductal cell (CL0002079)
    Fold Change: -0.0301
    Cell Significance Index: -3.4500
  • Cell Name: forebrain neuroblast (CL1000042)
    Fold Change: -0.0390
    Cell Significance Index: -2.4000
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: -0.0423
    Cell Significance Index: -4.3200
  • Cell Name: skeletal muscle fiber (CL0008002)
    Fold Change: -0.0424
    Cell Significance Index: -1.0900
  • Cell Name: mesonephric nephron tubule epithelial cell (CL1000022)
    Fold Change: -0.0489
    Cell Significance Index: -1.7000
  • Cell Name: pancreatic D cell (CL0000173)
    Fold Change: -0.0533
    Cell Significance Index: -11.2200
  • Cell Name: pigmented ciliary epithelial cell (CL0002303)
    Fold Change: -0.0597
    Cell Significance Index: -8.6800
  • Cell Name: epithelial cell of stomach (CL0002178)
    Fold Change: -0.0657
    Cell Significance Index: -7.6600
  • Cell Name: retinal rod cell (CL0000604)
    Fold Change: -0.0843
    Cell Significance Index: -1.0100
  • Cell Name: luminal adaptive secretory precursor cell of mammary gland (CL4033057)
    Fold Change: -0.0863
    Cell Significance Index: -4.0600
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: -0.0894
    Cell Significance Index: -5.7700
  • Cell Name: progenitor cell of mammary luminal epithelium (CL0009116)
    Fold Change: -0.1026
    Cell Significance Index: -7.6500
  • Cell Name: pancreatic endocrine cell (CL0008024)
    Fold Change: -0.1194
    Cell Significance Index: -13.6400
  • Cell Name: hippocampal pyramidal neuron (CL1001571)
    Fold Change: -0.1265
    Cell Significance Index: -3.6100
  • Cell Name: granulosa cell (CL0000501)
    Fold Change: -0.1318
    Cell Significance Index: -3.4700
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: -0.1410
    Cell Significance Index: -8.6500
  • Cell Name: intestinal crypt stem cell of small intestine (CL0009017)
    Fold Change: -0.1423
    Cell Significance Index: -3.0300
  • Cell Name: kidney epithelial cell (CL0002518)
    Fold Change: -0.1545
    Cell Significance Index: -4.5500
  • Cell Name: cerebellar granule cell (CL0001031)
    Fold Change: -0.1669
    Cell Significance Index: -2.8600
  • Cell Name: lung endothelial cell (CL1001567)
    Fold Change: -0.1695
    Cell Significance Index: -8.8300
  • Cell Name: kidney loop of Henle descending limb epithelial cell (CL1001021)
    Fold Change: -0.1760
    Cell Significance Index: -13.9400
  • Cell Name: smooth muscle cell of sphincter of pupil (CL0002243)
    Fold Change: -0.1855
    Cell Significance Index: -19.3200
  • Cell Name: intestinal epithelial cell (CL0002563)
    Fold Change: -0.1985
    Cell Significance Index: -2.0600
  • Cell Name: BEST4+ enteroycte (CL4030026)
    Fold Change: -0.2110
    Cell Significance Index: -3.1800
  • Cell Name: pro-T cell (CL0000827)
    Fold Change: -0.2630
    Cell Significance Index: -6.7200
  • Cell Name: fibroblast of dermis (CL0002551)
    Fold Change: -0.2675
    Cell Significance Index: -5.6000
  • Cell Name: pvalb GABAergic cortical interneuron (CL4023018)
    Fold Change: -0.2954
    Cell Significance Index: -6.2700
  • Cell Name: medium spiny neuron (CL1001474)
    Fold Change: -0.3027
    Cell Significance Index: -4.0900
  • Cell Name: peg cell (CL4033014)
    Fold Change: -0.3354
    Cell Significance Index: -7.7500
  • Cell Name: CD4-positive, alpha-beta memory T cell, CD45RO-positive (CL0001204)
    Fold Change: -0.3596
    Cell Significance Index: -10.5600
  • Cell Name: centrilobular region hepatocyte (CL0019029)
    Fold Change: -0.3609
    Cell Significance Index: -6.0800
  • Cell Name: epithelial cell of nephron (CL1000449)
    Fold Change: -0.3636
    Cell Significance Index: -3.0900
  • Cell Name: hepatoblast (CL0005026)
    Fold Change: -0.3651
    Cell Significance Index: -6.1400
  • Cell Name: cortical interneuron (CL0008031)
    Fold Change: -0.3724
    Cell Significance Index: -8.9300
  • Cell Name: Purkinje cell (CL0000121)
    Fold Change: -0.3804
    Cell Significance Index: -8.3300

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics:** DLAT is a mitochondrial matrix enzyme, specifically localized within the PDH complex. Its primary function is to catalyze the transfer of an acetyl group from coenzyme A (CoA) to dihydrolipoamide, resulting in the formation of acetyl-CoA. This reaction is a crucial step in the PDH reaction, which is critical for the oxidative decarboxylation of pyruvate and the subsequent production of acetyl-CoA. DLAT is also involved in other metabolic pathways, including glyoxylate metabolism and glycine degradation. **Pathways and Functions:** DLAT is integral to several metabolic pathways, including: 1. **Pyruvate Dehydrogenase Complex (PDH) Reaction:** DLAT is a key component of the PDH complex, which converts pyruvate to acetyl-CoA. This reaction is essential for the production of energy in the form of ATP, NADH, and FADH2. 2. **Glyoxylate Metabolism:** DLAT is involved in the metabolism of glyoxylate, a product of glycolysis and amino acid catabolism. Glyoxylate is converted to succinate via the enzyme glyoxylate reductase, which is then converted to succinate by the enzyme succinate synthetase. 3. **Glycine Degradation:** DLAT is also involved in the degradation of glycine, a non-essential amino acid. Glycine is converted to serine via the enzyme glycine decarboxylase, which is then converted to pyruvate via the enzyme serine hydroxymethyltransferase. **Clinical Significance:** DLAT plays a crucial role in various cellular contexts, including: 1. **Cardiac Metabolism:** DLAT is highly expressed in cardiac muscle cells, where it plays a key role in the regulation of pyruvate metabolism and energy production. 2. **Cancer Metabolism:** DLAT is overexpressed in various types of cancer, including breast, lung, and colon cancer. Its overexpression has been linked to increased energy production and tumor growth. 3. **Neurological Disorders:** DLAT has been implicated in the pathogenesis of neurological disorders, including Alzheimer's disease and Parkinson's disease. Its dysregulation has been linked to impaired energy production and neuronal function. In conclusion, DLAT is a multifunctional enzyme that plays a critical role in various metabolic pathways, including the PDH complex, glyoxylate metabolism, and glycine degradation. Its dysregulation has been linked to various diseases, highlighting the importance of understanding its role in cellular metabolism and signaling.

Genular Protein ID: 2143941255

Symbol: ODP2_HUMAN

Name: Pyruvate dehydrogenase complex component E2

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 16554811

Title: Human chromosome 11 DNA sequence and analysis including novel gene identification.

PubMed ID: 16554811

DOI: 10.1038/nature04632

PubMed ID: 3174635

Title: Primary structure of the human M2 mitochondrial autoantigen of primary biliary cirrhosis: dihydrolipoamide acetyltransferase.

PubMed ID: 3174635

DOI: 10.1073/pnas.85.19.7317

PubMed ID: 3191998

Title: Nucleotide sequence of a cDNA for the dihydrolipoamide acetyltransferase component of human pyruvate dehydrogenase complex.

PubMed ID: 3191998

DOI: 10.1016/0014-5793(88)80337-5

PubMed ID: 14638692

Title: Organization of the cores of the mammalian pyruvate dehydrogenase complex formed by E2 and E2 plus the E3-binding protein and their capacities to bind the E1 and E3 components.

PubMed ID: 14638692

DOI: 10.1074/jbc.m308172200

PubMed ID: 20160912

Title: Interaction of E1 and E3 components with the core proteins of the human pyruvate dehydrogenase complex.

PubMed ID: 20160912

DOI: 10.1016/j.molcatb.2009.05.001

PubMed ID: 19608861

Title: Lysine acetylation targets protein complexes and co-regulates major cellular functions.

PubMed ID: 19608861

DOI: 10.1126/science.1175371

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 25525879

Title: Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity.

PubMed ID: 25525879

DOI: 10.1016/j.cell.2014.11.046

PubMed ID: 25944712

Title: N-terminome analysis of the human mitochondrial proteome.

PubMed ID: 25944712

DOI: 10.1002/pmic.201400617

PubMed ID: 16049940

Title: Clinical and genetic spectrum of pyruvate dehydrogenase deficiency: dihydrolipoamide acetyltransferase (E2) deficiency.

PubMed ID: 16049940

DOI: 10.1002/ana.20550

PubMed ID: 9649469

Title: Three-dimensional structure of the major autoantigen in primary biliary cirrhosis.

PubMed ID: 9649469

DOI: 10.1016/s0016-5085(98)70375-0

PubMed ID: 15861126

Title: Crystal structure of pyruvate dehydrogenase kinase 3 bound to lipoyl domain 2 of human pyruvate dehydrogenase complex.

PubMed ID: 15861126

DOI: 10.1038/sj.emboj.7600663

PubMed ID: 17532006

Title: Crystal structure of an asymmetric complex of pyruvate dehydrogenase kinase 3 with lipoyl domain 2 and its biological implications.

PubMed ID: 17532006

DOI: 10.1016/j.jmb.2007.04.083

PubMed ID: 17683942

Title: Distinct structural mechanisms for inhibition of pyruvate dehydrogenase kinase isoforms by AZD7545, dichloroacetate, and radicicol.

PubMed ID: 17683942

DOI: 10.1016/j.str.2007.07.001

PubMed ID: 18387944

Title: Structural and functional insights into the molecular mechanisms responsible for the regulation of pyruvate dehydrogenase kinase 2.

PubMed ID: 18387944

DOI: 10.1074/jbc.m800311200

PubMed ID: 20361979

Title: Solution structure and characterisation of the human pyruvate dehydrogenase complex core assembly.

PubMed ID: 20361979

DOI: 10.1016/j.jmb.2010.03.043

Sequence Information:

  • Length: 647
  • Mass: 68997
  • Checksum: DD93A8E666E377C2
  • Sequence:
  • MWRVCARRAQ NVAPWAGLEA RWTALQEVPG TPRVTSRSGP APARRNSVTT GYGGVRALCG 
    WTPSSGATPR NRLLLQLLGS PGRRYYSLPP HQKVPLPSLS PTMQAGTIAR WEKKEGDKIN 
    EGDLIAEVET DKATVGFESL EECYMAKILV AEGTRDVPIG AIICITVGKP EDIEAFKNYT 
    LDSSAAPTPQ AAPAPTPAAT ASPPTPSAQA PGSSYPPHMQ VLLPALSPTM TMGTVQRWEK 
    KVGEKLSEGD LLAEIETDKA TIGFEVQEEG YLAKILVPEG TRDVPLGTPL CIIVEKEADI 
    SAFADYRPTE VTDLKPQVPP PTPPPVAAVP PTPQPLAPTP SAPCPATPAG PKGRVFVSPL 
    AKKLAVEKGI DLTQVKGTGP DGRITKKDID SFVPSKVAPA PAAVVPPTGP GMAPVPTGVF 
    TDIPISNIRR VIAQRLMQSK QTIPHYYLSI DVNMGEVLLV RKELNKILEG RSKISVNDFI 
    IKASALACLK VPEANSSWMD TVIRQNHVVD VSVAVSTPAG LITPIVFNAH IKGVETIAND 
    VVSLATKARE GKLQPHEFQG GTFTISNLGM FGIKNFSAII NPPQACILAI GASEDKLVPA 
    DNEKGFDVAS MMSVTLSCDH RVVDGAVGAQ WLAEFRKYLE KPITMLL

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.