Details for: GASK1B

Gene ID: 51313

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: GASK1B

Ensembl ID: ENSG00000164125

Description: golgi associated kinase 1B

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • Hofbauer cell CL3000001
    CSI 13.01
    rCSI 24.56%
    PRS 88.74
  • CD1c-positive myeloid dendritic cell CL0002399
    CSI 12.1
    rCSI 14.61%
    PRS 88.42
  • cardiac muscle cell CL0000746
    CSI 8.79
    rCSI 12.61%
    PRS 71.48
  • myoepithelial cell CL0000185
    CSI 8.1
    rCSI 20.49%
    PRS 86.14
  • stromal cell of ovary CL0002132
    CSI 6.27
    rCSI 17.22%
    PRS 87.62
  • Kupffer cell CL0000091
    CSI 4.38
    rCSI 10.02%
    PRS 82.26
  • CD14-positive, CD16-positive monocyte CL0002397
    CSI 3.47
    rCSI 4.55%
    PRS 91.16
  • renal interstitial pericyte CL1001318
    CSI 3.24
    rCSI 8.93%
    PRS 76.81
  • Schwann cell CL0002573
    CSI 3.15
    rCSI 8.94%
    PRS 77.2
  • myeloid leukocyte CL0000766
    CSI 2.96
    rCSI 2.73%
    PRS 82.99
  • intestine goblet cell CL0019031
    CSI 2.88
    rCSI 2.55%
    PRS 79.28
  • glial cell CL0000125
    CSI 2.77
    rCSI 10.56%
    PRS 72.81
  • blood vessel endothelial cell CL0000071
    CSI 2.6
    rCSI 5.4%
    PRS 78.27
  • neural crest cell CL0011012
    CSI 2.5
    rCSI 1.98%
    PRS 70.8
  • CD14-low, CD16-positive monocyte CL0002396
    CSI 2.49
    rCSI 1.91%
    PRS 84.41
  • inflammatory macrophage CL0000863
    CSI 2.4
    rCSI 4.1%
    PRS 94.44
  • CD14-positive monocyte CL0001054
    CSI 2.26
    rCSI 2.81%
    PRS 89.77
  • mesodermal cell CL0000222
    CSI 2.17
    rCSI 2.61%
    PRS 79.51
  • bronchus fibroblast of lung CL2000093
    CSI 2.1
    rCSI 1.71%
    PRS 80.82
  • retinal blood vessel endothelial cell CL0002585
    CSI 2.03
    rCSI 3.25%
    PRS 85
  • kidney interstitial alternatively activated macrophage CL1000695
    CSI 2.01
    rCSI 5.24%
    PRS 82.66
  • adventitial cell CL0002503
    CSI 1.99
    rCSI 4.74%
    PRS 85.18
  • Bergmann glial cell CL0000644
    CSI 1.93
    rCSI 2.64%
    PRS 73.02
  • elicited macrophage CL0000861
    CSI 1.82
    rCSI 1.67%
    PRS 88.6
  • endocardial cell CL0002350
    CSI 1.81
    rCSI 8.68%
    PRS 77.84
  • adipocyte CL0000136
    CSI 1.79
    rCSI 2.3%
    PRS 71.83
  • endothelial cell of uterus CL0009095
    CSI 1.78
    rCSI 13.05%
    PRS 89.03
  • alternatively activated macrophage CL0000890
    CSI 1.77
    rCSI 2.22%
    PRS 89.9
  • mononuclear phagocyte CL0000113
    CSI 1.68
    rCSI 3.7%
    PRS 85.05
  • intermediate monocyte CL0002393
    CSI 1.57
    rCSI 2.37%
    PRS 86.45
  • promonocyte CL0000559
    CSI 1.54
    rCSI 2.65%
    PRS 86.94
  • type EC enteroendocrine cell CL0000577
    CSI 1.54
    rCSI 5.45%
    PRS 85.19
  • cardiac endothelial cell CL0010008
    CSI 1.52
    rCSI 6.14%
    PRS 81.43
  • astrocyte of the cerebral cortex CL0002605
    CSI 1.5
    rCSI 3.37%
    PRS 64.78
  • vein endothelial cell CL0002543
    CSI 1.47
    rCSI 4.02%
    PRS 88.39
  • stem cell CL0000034
    CSI 1.38
    rCSI 1.33%
    PRS 75.12
  • glioblast CL0000030
    CSI 1.34
    rCSI 2.13%
    PRS 73.11
  • L6b glutamatergic cortical neuron CL4023038
    CSI 1.33
    rCSI 4.16%
    PRS 65.64
  • endothelial cell of pericentral hepatic sinusoid CL0019022
    CSI 1.29
    rCSI 3.98%
    PRS 85.6
  • basal cell CL0000646
    CSI 1.23
    rCSI 1.64%
    PRS 79.79
  • neural progenitor cell CL0011020
    CSI 1.1
    rCSI 4.82%
    PRS 70
  • lung macrophage CL1001603
    CSI 1.1
    rCSI 2.45%
    PRS 88.13
  • prostate gland microvascular endothelial cell CL2000059
    CSI 1.08
    rCSI 25.81%
    PRS 89.41
  • fibroblast of cardiac tissue CL0002548
    CSI 1.07
    rCSI 5.12%
    PRS 81.44
  • mesenchymal cell CL0008019
    CSI 1.05
    rCSI 2.67%
    PRS 75.08
  • vasa recta ascending limb cell CL1001131
    CSI 1.04
    rCSI 4.69%
    PRS 88.81
  • endothelial cell of arteriole CL1000412
    CSI 1.02
    rCSI 5.63%
    PRS 89.74
  • CD14-positive, CD16-negative classical monocyte CL0002057
    CSI 1
    rCSI 6.06%
    PRS 91.53
  • stromal cell CL0000499
    CSI 0.98
    rCSI 2.77%
    PRS 76.71
  • vein endothelial cell of respiratory system CL4033008
    CSI 0.92
    rCSI 6.32%
    PRS 87.66
  • endothelial cell of placenta CL0009092
    CSI 0.79
    rCSI 3.89%
    PRS 88.46
  • colon macrophage CL0009038
    CSI 0.77
    rCSI 3.56%
    PRS 92.21
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 0.73
    rCSI 2.76%
    PRS 64.54
  • collagen secreting cell CL0000667
    CSI 0.71
    rCSI 4.08%
    PRS 86.88
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 0.69
    rCSI 1.68%
    PRS 61.96
  • endothelial cell of venule CL1000414
    CSI 0.69
    rCSI 6.11%
    PRS 89
  • endothelial cell of vascular tree CL0002139
    CSI 0.56
    rCSI 3.04%
    PRS 77.95
  • pancreatic stellate cell CL0002410
    CSI 0.54
    rCSI 3.12%
    PRS 84.89
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 0.34
    rCSI 2.01%
    PRS 64.63
  • mesenchymal stem cell CL0000134
    CSI 0.2
    rCSI 2.17%
    PRS 86.39

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [GASK1B](/details-gene/51313) (Golgi Associated Kinase 1B) is a protein-coding gene located on chromosome 4q32.1. As its name suggests, and supported by functional annotations, this protein is primarily localized to the [Golgi apparatus](/details-ontology/GO0005794) and [Golgi membrane](/details-ontology/GO0000139). Initial identification of this gene emerged from large-scale cDNA sequencing and protein discovery initiatives [Link](https://doi.org/10.1101/gr.1293003) [Link](https://doi.org/10.1038/ng1285). **Overall**, expression data indicate that [GASK1B](/details-gene/51313) shows significant expression in a diverse array of cell types, with particularly high significance in cells of the myeloid lineage, including [Hofbauer cell](/details-cell/CL3000001) and [CD1c-positive myeloid dendritic cell](/details-cell/CL0002399), as well as in contractile cells such as [cardiac muscle cell](/details-cell/CL0000746). This pattern suggests a fundamental role in Golgi-mediated processes that are crucial across multiple, functionally distinct tissues. ## Cellular Roles and Expression Landscape The expression profile of [GASK1B](/details-gene/51313) points to a significant role in several distinct cellular systems. The gene's highest significance index (**Overall** context) is observed in professional phagocytes and antigen-presenting cells of the myeloid lineage. This includes placental macrophages ([Hofbauer cell](/details-cell/CL3000001); CSI: 13.01), [CD1c-positive myeloid dendritic cell](/details-cell/CL0002399) (CSI: 12.10), liver-resident macrophages ([Kupffer cell](/details-cell/CL0000091); CSI: 4.38), and circulating [CD14-positive, CD16-positive monocyte](/details-cell/CL0002397) (CSI: 3.47). This consistent enrichment across multiple myeloid subtypes suggests [GASK1B](/details-gene/51313) is likely involved in core functions of these immune cells, such as protein secretion (e.g., cytokines) or membrane trafficking associated with phagocytosis and antigen presentation. Concurrently, [GASK1B](/details-gene/51313) is also a highly significant gene in non-immune, specialized cell types. It shows a strong signal in [cardiac muscle cell](/details-cell/CL0000746) (CSI: 8.79) and [myoepithelial cell](/details-cell/CL0000185) (CSI: 8.10), both of which are characterized by contractile activity and extensive membrane systems. This dual-expression pattern in both myeloid and contractile cells may indicate its involvement in a shared fundamental process, potentially the regulation of trafficking for secreted or transmembrane proteins that are vital for the unique functions of each cell type. The gene also shows notable expression in secretory cells like [intestine goblet cell](/details-cell/CL0019031) and supportive cells such as [Schwann cell](/details-cell/CL0002573), further highlighting its broad importance in cells with high metabolic or secretory activity. ## Pathways and Molecular Function The known functional annotations for [GASK1B](/details-gene/51313) are highly specific, placing it within the [Golgi apparatus](/details-ontology/GO0005794) and integral to the [Golgi membrane](/details-ontology/GO0000139). As a putative kinase, its function is likely to involve the phosphorylation of substrate proteins within this organelle. The Golgi complex is a central hub for post-translational modification, sorting, and packaging of proteins and lipids for secretion or delivery to other organelles. This molecular function is consistent with its high expression in the aforementioned cell types. In myeloid cells like dendritic cells and macrophages, efficient Golgi function is critical for processing and secreting cytokines and chemokines to orchestrate immune responses. In [cardiac muscle cell](/details-cell/CL0000746), the Golgi is essential for processing proteins destined for the sarcolemma and sarcoplasmic reticulum, which are critical for excitation-contraction coupling. Therefore, the kinase activity of [GASK1B](/details-gene/51313) may represent a key regulatory node in the secretory and protein trafficking pathways of these diverse cells. ## Research Directions The widespread yet specific expression of [GASK1B](/details-gene/51313) in functionally critical cell types presents several avenues for future research, particularly concerning its role in both immunology and muscle physiology. **Proposed Testable Hypotheses:** 1. **[GASK1B](/details-gene/51313) is a critical regulator of cytokine and chemokine secretion in myeloid cells.** Given its high expression in macrophages, monocytes, and dendritic cells, and its localization to the Golgi, it is plausible that [GASK1B](/details-gene/51313) phosphorylates key trafficking proteins (e.g., Rabs, SNAREs) or cytokine precursors, thereby controlling their transport and release during an inflammatory response. 2. **[GASK1B](/details-gene/51313) activity is essential for the proper trafficking and membrane localization of ion channels or structural proteins in cardiomyocytes.** Its high significance in [cardiac muscle cell](/details-cell/CL0000746) suggests a role beyond immunity. Dysregulation of [GASK1B](/details-gene/51313) could disrupt the processing of proteins vital for cardiac function, potentially contributing to cardiomyopathies. **Suggested Experimental Approach:** To test the hypothesis regarding its role in myeloid cell secretion (Hypothesis 1), a robust experimental plan could be implemented. CRISPR-Cas9 could be used to knock out [GASK1B](/details-gene/51313) in a human monocyte cell line (e.g., THP-1). Following differentiation into macrophage-like cells, both knockout and wild-type control cells would be stimulated with an inflammatory agonist like lipopolysaccharide (LPS). The secretion of key cytokines (e.g., TNF-alpha, IL-6, IL-1beta) into the supernatant would be quantified by ELISA. A significant reduction in cytokine secretion in the knockout cells would support the hypothesis. To identify direct substrates, phosphoproteomic analysis could be performed on Golgi-enriched fractions from stimulated wild-type versus knockout cells. **Therapeutic Potential:** As a kinase, [GASK1B](/details-gene/51313) is a potentially druggable target. If its activity is shown to be upregulated in inflammatory disorders or autoimmune diseases, leading to pathologic cytokine secretion from myeloid cells, it could represent a novel target for therapeutic **inhibition**. The development of specific small-molecule inhibitors targeting the ATP-binding pocket of [GASK1B](/details-gene/51313) could offer a new strategy to dampen excessive inflammation. However, its high expression in other critical tissues, such as the heart, raises potential safety concerns regarding off-target toxicity, which would require careful evaluation in any drug development program.

Genular Protein ID: 486658871

Symbol: GAK1B_HUMAN

Name: N/A

UniProtKB Accession Codes:

Q6UWH4 Q498Z3 Q6IAF9 Q6ZMF2 Q86XL0

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 2 CTD 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 5 Ensembl 4 ExpressionAtlas 1 GO 2 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyCosmos 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 1 KEGG 1 MANE-Select 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 3 RNAct 1 RefSeq 3 STRING 1 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 12975309

Title: The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.

PubMed ID: 12975309

DOI: 10.1101/gr.1293003

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 17974005

Title: The full-ORF clone resource of the German cDNA consortium.

PubMed ID: 17974005

DOI: 10.1186/1471-2164-8-399

PubMed ID: 11256614

Title: Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.

PubMed ID: 11256614

DOI: 10.1093/embo-reports/kvd058

Sequence Information:

  • Length: 519
  • Mass: 57552
  • Checksum: 94F6EE648E4CB0BD
  • Sequence:
    • MTCPDKPGQL INWFICSLCV PRVRKLWSSR RPRTRRNLLL GTACAIYLGF LVSQVGRASL 
QHGQAAEKGP HRSRDTAEPS FPEIPLDGTL APPESQGNGS TLQPNVVYIT LRSKRSKPAN 
IRGTVKPKRR KKHAVASAAP GQEALVGPSL QPQEAAREAD AVAPGYAQGA NLVKIGERPW 
RLVRGPGVRA GGPDFLQPSS RESNIRIYSE SAPSWLSKDD IRRMRLLADS AVAGLRPVSS 
RSGARLLVLE GGAPGAVLRC GPSPCGLLKQ PLDMSEVFAF HLDRILGLNR TLPSVSRKAE 
FIQDGRPCPI ILWDASLSSA SNDTHSSVKL TWGTYQQLLK QKCWQNGRVP KPESGCTEIH 
HHEWSKMALF DFLLQIYNRL DTNCCGFRPR KEDACVQNGL RPKCDDQGSA ALAHIIQRKH 
DPRHLVFIDN KGFFDRSEDN LNFKLLEGIK EFPASAVSVL KSQHLRQKLL QSLFLDKVYW 
ESQGGRQGIE KLIDVIEHRA KILITYINAH GVKVLPMNE

Genular Protein ID: 2838542520

Symbol: Q9NYZ0_HUMAN

Name: N/A

UniProtKB Accession Codes:

Q9NYZ0

Database IDs:

ARBA 2 AlphaFoldDB 1 BioGRID-ORCS 1 CTD 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 6 GO 1 InterPro 1 KEGG 1 NCBI Taxonomy 1 OrthoDB 1 PANTHER 2 PeptideAtlas 1 Pfam 1 PharmGKB 1 RefSeq 3

Citations:

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 246
  • Mass: 28233
  • Checksum: 90BF8959053E896D
  • Sequence:
    • MSEVFAFHLD RILGLNRTLP SVSRKAEFIQ DGRPCPIILW DASLSSASND THSSVKLTWG 
TYQQLLKQKC WQNGRVPKPE SGCTEIHHHE WSKMALFDFL LQIYNRLDTN CCGFRPRKED 
ACVQNGLRPK CDDQGSAALA HIIQRKHDPR HLVFIDNKGF FDRSEDNLNF KLLEGIKEFP 
ASAVSVLKSQ HLRQKLLQSL FLDKVYWESQ GGRQGIEKLI DVIEHRAKIL ITYINAHGVK 
VLPMNE