Details for: TMEM8B

Gene ID: 51754

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: TMEM8B

Ensembl ID: ENSG00000137103

Description: transmembrane protein 8B

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • myoepithelial cell CL0000185
    CSI 3.83
    rCSI 9.68%
    PRS 96.55
  • plasmacytoid dendritic cell, human CL0001058
    CSI 3.52
    rCSI 2.46%
    PRS 97.16
  • ciliated epithelial cell CL0000067
    CSI 3.31
    rCSI 2.91%
    PRS 89.33
  • neural crest cell CL0011012
    CSI 3.09
    rCSI 2.44%
    PRS 91.53
  • kidney loop of Henle thin descending limb epithelial cell CL1001111
    CSI 3.03
    rCSI 4.29%
    PRS 94.14
  • lung ciliated cell CL1000271
    CSI 2.77
    rCSI 3.21%
    PRS 91.69
  • intestinal epithelial cell CL0002563
    CSI 2.77
    rCSI 2.89%
    PRS 93.65
  • enteric smooth muscle cell CL0002504
    CSI 2.61
    rCSI 3.72%
    PRS 95.47
  • placental villous trophoblast CL2000060
    CSI 2.48
    rCSI 3.84%
    PRS 94
  • dendritic cell CL0000451
    CSI 2.36
    rCSI 2.91%
    PRS 94.72
  • cerebral cortex neuron CL0010012
    CSI 2.29
    rCSI 9.33%
    PRS 90.03
  • VIP GABAergic cortical interneuron CL4023016
    CSI 2.28
    rCSI 2.73%
    PRS 87.6
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 2.22
    rCSI 2.76%
    PRS 85.72
  • sst GABAergic cortical interneuron CL4023017
    CSI 2.09
    rCSI 2.7%
    PRS 88.48
  • cardiac muscle cell CL0000746
    CSI 2.02
    rCSI 2.9%
    PRS 89.84
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 1.98
    rCSI 3.33%
    PRS 87.65
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 1.85
    rCSI 3.27%
    PRS 87.19
  • tuft cell of colon CL0009041
    CSI 1.49
    rCSI 3.46%
    PRS 95.75
  • intestinal crypt stem cell of small intestine CL0009017
    CSI 1.46
    rCSI 3.93%
    PRS 96.14
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 0.96
    rCSI 2.33%
    PRS 85.75
  • dopaminergic neuron CL0000700
    CSI 0.94
    rCSI 5.3%
    PRS 88.15
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 0.58
    rCSI 2.07%
    PRS 86
  • indirect pathway medium spiny neuron CL4023029
    CSI 0.33
    rCSI 8.08%
    PRS 85.16
  • direct pathway medium spiny neuron CL4023026
    CSI 0.3
    rCSI 7.19%
    PRS 85.35

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary Transmembrane protein 8B, encoded by the [TMEM8B](/details-gene/51754) gene, is a protein with reported localization to the plasma membrane and various intracellular organelles. Functional annotations link it to fundamental cellular processes including [cell-matrix adhesion](/details-cell/GO:0007160) and the [regulation of the mitotic cell cycle](/details-cell/GO:0007346). Expression data indicates its significance across a diverse array of cell types, with particularly high scores in [myoepithelial cells](/details-cell/CL0000185), [plasmacytoid dendritic cells, human](/details-cell/CL0001058), and various ciliated and epithelial cells. Research, particularly under its alias NGX6, suggests a role as a tumor suppressor in nasopharyngeal carcinoma by modulating cell adhesion and proliferation ([Link](https://doi.org/10.1093/carcin/bgh312), [Link](https://doi.org/10.1002/jcb.20393)). ## Cellular Roles and Expression Landscape The **Overall** expression profile of [TMEM8B](/details-gene/51754) highlights its importance across multiple distinct cell lineages, suggesting a broad yet context-specific functional role. The gene shows its highest significance in epithelial and epithelial-like cells, including [myoepithelial cell](/details-cell/CL0000185) (CSI: 3.83), [ciliated epithelial cell](/details-cell/CL0000067) (CSI: 3.31), [kidney loop of Henle thin descending limb epithelial cell](/details-cell/CL1001111) (CSI: 3.03), and [lung ciliated cell](/details-cell/CL1000271) (CSI: 2.77). This pattern is consistent with its annotated function in [cell-matrix adhesion](/details-cell/GO:0007160), a critical process for tissue structure and integrity in epithelial layers. Notably, [TMEM8B](/details-gene/51754) is also a significant marker in the immune system, particularly in [plasmacytoid dendritic cells, human](/details-cell/CL0001058) (CSI: 3.52) and, more broadly, [dendritic cells](/details-cell/CL0000451) (CSI: 2.36). This suggests a potential, albeit less characterized, role in immune surveillance or antigen presentation. Furthermore, the gene is expressed in diverse cell types such as [neural crest cells](/details-cell/CL0011012) (CSI: 3.09), various [cerebral cortex neurons](/details-cell/CL0010012), and muscle tissues like [enteric smooth muscle cells](/details-cell/CL0002504) and [cardiac muscle cells](/details-cell/CL0000746). This widespread expression pattern indicates that [TMEM8B](/details-gene/51754) may be involved in fundamental cellular functions common to many tissues, such as cell cycle control and adhesion, rather than being restricted to a single lineage. ## Pathways and Molecular Function Functional annotations indicate [TMEM8B](/details-gene/51754) participates in core cellular machinery. Its involvement in [cell-matrix adhesion](/details-cell/GO:0007160) aligns with its localization to the [cell surface](/details-cell/GO:0009986) and [plasma membrane](/details-cell/GO:0005886) and is strongly supported by research demonstrating its ability to modulate cell adhesion in nasopharyngeal carcinoma cells ([Link](https://doi.org/10.1093/carcin/bgh312)). The gene is also linked to the [regulation of the mitotic cell cycle](/details-cell/GO:0007346), a function corroborated by studies showing that its expression, as NGX6, inhibits cell proliferation and negatively regulates the EGFR pathway ([Link](https://doi.org/10.1002/jcb.20393)). Its broad intracellular localization, including the [nucleus](/details-cell/GO:0005634), [endoplasmic reticulum](/details-cell/GO:0005783), and [mitochondrion](/details-cell/GO:0005739), coupled with its function in [protein binding](/details-cell/GO:0005515), suggests it may act as a scaffold or adaptor protein that integrates signals from the cell surface to intracellular pathways controlling cell division and survival. One study identified an interaction with ezrin, a protein linking the cytoskeleton to the plasma membrane, further supporting its role in adhesion and signaling ([Link](https://doi.org/10.1111/j.1349-7006.2007.00410.x)). ## Research Directions The existing literature strongly implicates [TMEM8B](/details-gene/51754) (as NGX6) as a candidate tumor suppressor, particularly in nasopharyngeal carcinoma. Future research should focus on elucidating the precise molecular mechanisms behind this function and exploring its relevance in other malignancies and cellular contexts. **Testable Hypotheses:** 1. Downregulation or loss of [TMEM8B](/details-gene/51754) in epithelial cancers directly promotes an epithelial-to-mesenchymal transition (EMT) by disrupting cell-matrix adhesion complexes and releasing constraints on cell migration. 2. [TMEM8B](/details-gene/51754) functions as a negative regulator of the cell cycle by physically interacting with and sequestering key components of growth factor signaling pathways, such as adaptors downstream of EGFR, thereby preventing their activation. 3. In [plasmacytoid dendritic cells](/details-cell/CL0001058), [TMEM8B](/details-gene/51754) is critical for cell adhesion and migration to lymph nodes, and its absence impairs the initiation of adaptive immune responses. **Proposed Experiment:** To test the first hypothesis, one could utilize CRISPR-Cas9 to knock out [TMEM8B](/details-gene/51754) in a non-metastatic epithelial cancer cell line (e.g., MCF-7). The resulting knockout cells and wild-type controls would be analyzed for changes in cell morphology, expression of EMT markers (e.g., E-cadherin, N-cadherin, Vimentin) via western blot and qRT-PCR, and functional changes in adhesion to extracellular matrix components (e.g., fibronectin, collagen) and migratory/invasive potential using Transwell assays. **Therapeutic Potential:** Given that multiple studies suggest [TMEM8B](/details-gene/51754) functions as a tumor suppressor whose expression is lost in cancer ([Link](https://doi.org/10.1002/jcb.20393), [Link](https://doi.org/10.1159/000106073)), the therapeutic strategy would focus on activation or restoration rather than inhibition. For tumors characterized by epigenetic silencing or deletion of [TMEM8B](/details-gene/51754), therapeutic approaches could include gene therapy to reintroduce a functional copy of the gene or the development of small-molecule drugs that reactivate its expression or mimic its downstream tumor-suppressive functions.

Genular Protein ID: 2848664852

Symbol: TMM8B_HUMAN

Name: Transmembrane protein 8B

UniProtKB Accession Codes:

A6NDV4 B3KQF3 O75539 Q49AB1 Q4KMX5 Q5TCW5 Q9HBY2 Q9P0U7

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 2 CTD 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 10 Ensembl 5 ExpressionAtlas 1 GO 7 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyCosmos 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 2 KEGG 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 3 RNAct 1 RefSeq 3 SMR 1 STRING 1 SignaLink 1 TCDB 1 TopDownProteomics 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 11565907

Title: Proteomic detection of changes in protein synthesis induced by NGX6 transfected in human nasopharyngeal carcinoma cells.

PubMed ID: 11565907

DOI: 10.1023/a:1010912311564

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15164053

Title: DNA sequence and analysis of human chromosome 9.

PubMed ID: 15164053

DOI: 10.1038/nature02465

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 15498789

Title: Role of a novel EGF-like domain-containing gene NGX6 in cell adhesion modulation in nasopharyngeal carcinoma cells.

PubMed ID: 15498789

DOI: 10.1093/carcin/bgh312

PubMed ID: 15723283

Title: NGX6 gene inhibits cell proliferation and plays a negative role in EGFR pathway in nasopharyngeal carcinoma cells.

PubMed ID: 15723283

DOI: 10.1002/jcb.20393

PubMed ID: 17641538

Title: The role of NGX6 and its deletion mutants in the proliferation, adhesion and migration of nasopharyngeal carcinoma 5-8F cells.

PubMed ID: 17641538

DOI: 10.1159/000106073

PubMed ID: 17270023

Title: The expression of ezrin in NPC and its interaction with NGX6, a novel candidate suppressor.

PubMed ID: 17270023

DOI: 10.1111/j.1349-7006.2007.00410.x

Sequence Information:

  • Length: 472
  • Mass: 51941
  • Checksum: 3E5EA4495CF54389
  • Sequence:
    • MNMPQSLGNQ PLPPEPPSLG TPAEGPGTTS PPEHCWPVRP TLRNELDTFS VHFYIFFGPS 
VALPPERPAV FAMRLLPVLD SGGVLSLELQ LNASSVRQEN VTVFGCLTHE VPLSLGDAAV 
TCSKESLAGF LLSVSATTRV ARLRIPFPQT GTWFLALRSL CGVGPRFVRC RNATAEVRMR 
TFLSPCVDDC GPYGQCKLLR THNYLYAACE CKAGWRGWGC TDSADALTYG FQLLSTLLLC 
LSNLMFLPPV VLAIRSRYVL EAAVYTFTMF FSTFYHACDQ PGIVVFCIMD YDVLQFCDFL 
GSLMSVWVTV IAMARLQPVV KQVLYLLGAM LLSMALQLDR HGLWNLLGPS LFALGILATA 
WTVRSVRRRH CYPPTWRRWL FYLCPGSLIA GSAVLLYAFV ETRDNYFYIH SIWHMLIAGS 
VGFLLPPRAK TDHGVPSGAR ARGCGYQLCI NEQEELGLVG PGGATVSSIC AS