Details for: ROBO2

Gene ID: 6092

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ROBO2

Ensembl ID: ENSG00000185008

Description: roundabout guidance receptor 2

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • VIP GABAergic cortical interneuron CL4023016
    CSI 53.55
    rCSI 63.96%
    PRS 92.37
  • sst GABAergic cortical interneuron CL4023017
    CSI 49.26
    rCSI 63.5%
    PRS 92.95
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 47.82
    rCSI 59.49%
    PRS 91.06
  • retinal bipolar neuron CL0000748
    CSI 38.3
    rCSI 71.74%
    PRS 93.49
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 35.51
    rCSI 62.72%
    PRS 92.36
  • sncg GABAergic cortical interneuron CL4023015
    CSI 34.15
    rCSI 54.92%
    PRS 92.64
  • inhibitory interneuron CL0000498
    CSI 33.13
    rCSI 76.49%
    PRS 93.26
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 32.87
    rCSI 55.18%
    PRS 92.6
  • L2/3 intratelencephalic projecting glutamatergic neuron CL4030059
    CSI 28.42
    rCSI 61.66%
    PRS 90.59
  • L4 intratelencephalic projecting glutamatergic neuron CL4030063
    CSI 24.15
    rCSI 57.77%
    PRS 91.9
  • ependymal cell CL0000065
    CSI 23.43
    rCSI 47.54%
    PRS 88.1
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 22.35
    rCSI 25.81%
    PRS 93.81
  • neuron CL0000540
    CSI 21.51
    rCSI 57.28%
    PRS 89.88
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 21.45
    rCSI 27.53%
    PRS 95.99
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 21.4
    rCSI 52.01%
    PRS 90.97
  • epithelial cell of proximal tubule CL0002306
    CSI 20.67
    rCSI 50.47%
    PRS 94
  • cerebral cortex endothelial cell CL1001602
    CSI 19.93
    rCSI 34.46%
    PRS 95.47
  • glycinergic amacrine cell CL4030028
    CSI 19.9
    rCSI 51.84%
    PRS 93.44
  • myofibroblast cell CL0000186
    CSI 19.61
    rCSI 27.16%
    PRS 96.15
  • L6b glutamatergic cortical neuron CL4023038
    CSI 19.39
    rCSI 60.58%
    PRS 92.91
  • L5/6 near-projecting glutamatergic neuron CL4030067
    CSI 19.12
    rCSI 62.85%
    PRS 91
  • interneuron CL0000099
    CSI 17.98
    rCSI 36.1%
    PRS 95.24
  • adipocyte CL0000136
    CSI 17.45
    rCSI 22.41%
    PRS 93.75
  • fibroblast of lung CL0002553
    CSI 17.42
    rCSI 16.21%
    PRS 98.37
  • rod bipolar cell CL0000751
    CSI 17.11
    rCSI 30.74%
    PRS 94.85
  • peripheral nervous system neuron CL2000032
    CSI 16.74
    rCSI 22.8%
    PRS 94.69
  • intestine goblet cell CL0019031
    CSI 16.49
    rCSI 14.64%
    PRS 96.38
  • goblet cell CL0000160
    CSI 16.42
    rCSI 15.51%
    PRS 96.37
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 15.65
    rCSI 56.3%
    PRS 91.14
  • vascular leptomeningeal cell CL4023051
    CSI 15.6
    rCSI 27.34%
    PRS 95.87
  • bronchus fibroblast of lung CL2000093
    CSI 15.33
    rCSI 12.45%
    PRS 97.6
  • choroid plexus epithelial cell CL0000706
    CSI 15.24
    rCSI 24.96%
    PRS 94.74
  • chandelier pvalb GABAergic cortical interneuron CL4023036
    CSI 15.12
    rCSI 47.3%
    PRS 93.67
  • GABAergic amacrine cell CL4030027
    CSI 14.84
    rCSI 50.83%
    PRS 90.37
  • alveolar type 1 fibroblast cell CL4028004
    CSI 14.66
    rCSI 16.05%
    PRS 98.31
  • glutamatergic neuron CL0000679
    CSI 14.56
    rCSI 29.91%
    PRS 90.54
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 14.18
    rCSI 53.59%
    PRS 92.16
  • alveolar adventitial fibroblast CL4028006
    CSI 13.99
    rCSI 22.1%
    PRS 98.17
  • GABAergic neuron CL0000617
    CSI 13.91
    rCSI 46.61%
    PRS 90.04
  • renal interstitial pericyte CL1001318
    CSI 13.84
    rCSI 38.14%
    PRS 96.93
  • parietal epithelial cell CL1000452
    CSI 13.8
    rCSI 36.89%
    PRS 95.67
  • retinal ganglion cell CL0000740
    CSI 13.45
    rCSI 29.72%
    PRS 92.44
  • blood vessel endothelial cell CL0000071
    CSI 12.86
    rCSI 26.69%
    PRS 96.76
  • H1 horizontal cell CL0004217
    CSI 12.69
    rCSI 50.27%
    PRS 93.57
  • serotonergic neuron CL0000850
    CSI 12.66
    rCSI 56.57%
    PRS 88.46
  • radial glial cell CL0000681
    CSI 12.54
    rCSI 17.42%
    PRS 97.1
  • neural crest cell CL0011012
    CSI 12.33
    rCSI 9.75%
    PRS 95.45
  • glioblast CL0000030
    CSI 12.27
    rCSI 19.58%
    PRS 93.97
  • hepatic stellate cell CL0000632
    CSI 12.26
    rCSI 45.94%
    PRS 96.28
  • vascular associated smooth muscle cell CL0000359
    CSI 12.03
    rCSI 39.01%
    PRS 97.24
  • cerebral cortex neuron CL0010012
    CSI 11.9
    rCSI 48.48%
    PRS 92.88
  • kidney collecting duct principal cell CL1001431
    CSI 11.81
    rCSI 59.46%
    PRS 95.45
  • progenitor cell CL0011026
    CSI 11.38
    rCSI 24.21%
    PRS 94.02
  • amacrine cell CL0000561
    CSI 11.29
    rCSI 32.73%
    PRS 93.29
  • invaginating midget bipolar cell CL4033034
    CSI 11.16
    rCSI 65.92%
    PRS 91.69
  • Bergmann glial cell CL0000644
    CSI 11.16
    rCSI 15.27%
    PRS 93.8
  • cerebral cortex GABAergic interneuron CL0010011
    CSI 10.99
    rCSI 32.44%
    PRS 97.13
  • Mueller cell CL0000636
    CSI 10.94
    rCSI 24.97%
    PRS 94.61
  • neural cell CL0002319
    CSI 10.81
    rCSI 40.79%
    PRS 89.88
  • diffuse bipolar 6 cell CL4033032
    CSI 10.59
    rCSI 55.7%
    PRS 91.15
  • macroglial cell CL0000126
    CSI 10.36
    rCSI 26.64%
    PRS 95.33
  • dopaminergic neuron CL0000700
    CSI 10.35
    rCSI 58.47%
    PRS 91.76
  • cerebellar granule cell CL0001031
    CSI 10.33
    rCSI 15.19%
    PRS 94.83
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 9.53
    rCSI 56.09%
    PRS 92.43
  • retinal pigment epithelial cell CL0002586
    CSI 9.42
    rCSI 18.71%
    PRS 95.68
  • cardiac muscle cell CL0000746
    CSI 9.21
    rCSI 13.22%
    PRS 93.17
  • H2 horizontal cell CL0004218
    CSI 9.15
    rCSI 45.47%
    PRS 93.95
  • basal cell CL0000646
    CSI 9
    rCSI 12.03%
    PRS 96.08
  • astrocyte of the cerebral cortex CL0002605
    CSI 8.69
    rCSI 19.48%
    PRS 92.74
  • tracheobronchial smooth muscle cell CL0019019
    CSI 8.2
    rCSI 14.46%
    PRS 98.24
  • contractile cell CL0000183
    CSI 8.13
    rCSI 24%
    PRS 97.55
  • mesenchymal cell CL0008019
    CSI 7.96
    rCSI 20.2%
    PRS 96.23
  • erythroblast CL0000765
    CSI 7.81
    rCSI 20.73%
    PRS 97.06
  • kidney interstitial alternatively activated macrophage CL1000695
    CSI 7.78
    rCSI 20.28%
    PRS 98.38
  • central nervous system neuron CL2000029
    CSI 7.77
    rCSI 57.13%
    PRS 93.72
  • mature astrocyte CL0002627
    CSI 7.53
    rCSI 32.02%
    PRS 94.41
  • kidney connecting tubule epithelial cell CL1000768
    CSI 7.4
    rCSI 18.77%
    PRS 95.24
  • chondrocyte CL0000138
    CSI 7.14
    rCSI 11.35%
    PRS 95.53
  • pancreatic A cell CL0000171
    CSI 7.03
    rCSI 7.36%
    PRS 97.78
  • retina horizontal cell CL0000745
    CSI 6.81
    rCSI 10.38%
    PRS 95.77
  • medium spiny neuron CL1001474
    CSI 6.77
    rCSI 58.34%
    PRS 93.3
  • podocyte CL0000653
    CSI 6.63
    rCSI 29.46%
    PRS 97.47
  • basal cell of epidermis CL0002187
    CSI 6.61
    rCSI 11.72%
    PRS 78.26
  • diffuse bipolar 1 cell CL4033027
    CSI 6.24
    rCSI 46.88%
    PRS 90.5
  • conjunctival epithelial cell CL1000432
    CSI 6.23
    rCSI 9.52%
    PRS 96.66
  • mesangial cell CL0000650
    CSI 5.92
    rCSI 24.12%
    PRS 98.61
  • cardiac blood vessel endothelial cell CL0010006
    CSI 5.8
    rCSI 40.99%
    PRS 93.88
  • kidney interstitial fibroblast CL1000692
    CSI 5.77
    rCSI 30.68%
    PRS 94.64
  • mesodermal cell CL0000222
    CSI 5.71
    rCSI 6.85%
    PRS 97.88
  • diffuse bipolar 4 cell CL4033031
    CSI 5.65
    rCSI 64.76%
    PRS 88.09
  • stromal cell CL0000499
    CSI 5.4
    rCSI 15.19%
    PRS 96.18
  • stem cell CL0000034
    CSI 5.38
    rCSI 5.19%
    PRS 96.34
  • regular atrial cardiac myocyte CL0002129
    CSI 5.26
    rCSI 16.92%
    PRS 94.97
  • melanocyte of skin CL1000458
    CSI 5.06
    rCSI 6.89%
    PRS 79.36
  • endothelial cell of vascular tree CL0002139
    CSI 4.71
    rCSI 25.76%
    PRS 95.25
  • kidney loop of Henle thick ascending limb epithelial cell CL1001106
    CSI 4.69
    rCSI 40.53%
    PRS 94.32
  • starburst amacrine cell CL0004232
    CSI 4.62
    rCSI 38.86%
    PRS 88.85
  • ON parasol ganglion cell CL4033052
    CSI 4.32
    rCSI 61.37%
    PRS 92.88
  • retinal cone cell CL0000573
    CSI 4.26
    rCSI 6.86%
    PRS 93.43
  • differentiation-committed oligodendrocyte precursor CL4023059
    CSI 4.26
    rCSI 7.74%
    PRS 94.31
  • blood vessel smooth muscle cell CL0019018
    CSI 0.8
    rCSI 6.9%
    PRS 97.1%
  • kidney distal convoluted tubule epithelial cell CL1000849
    CSI 1.3
    rCSI 14.0%
    PRS 95.3%
  • mesenchymal stem cell CL0000134
    CSI 1.4
    rCSI 15.3%
    PRS 98.3%
  • diffuse bipolar 3a cell CL4033029
    CSI 1.4
    rCSI 9.5%
    PRS 92.1%
  • S cone cell CL0003050
    CSI 2.0
    rCSI 8.8%
    PRS 94.7%
  • type B pancreatic cell CL0000169
    CSI 2.0
    rCSI 4.5%
    PRS 97.2%
  • corneal epithelial cell CL0000575
    CSI 2.2
    rCSI 6.3%
    PRS 97.4%
  • intestinal epithelial cell CL0002563
    CSI 2.3
    rCSI 2.4%
    PRS 96.3%
  • neuroplacodal cell CL0000032
    CSI 2.3
    rCSI 21.4%
    PRS 94.2%
  • indirect pathway medium spiny neuron CL4023029
    CSI 2.4
    rCSI 57.4%
    PRS 90.0%
  • direct pathway medium spiny neuron CL4023026
    CSI 2.4
    rCSI 57.6%
    PRS 90.2%
  • Cajal-Retzius cell CL0000695
    CSI 2.6
    rCSI 20.3%
    PRS 97.2%
  • glial cell CL0000125
    CSI 2.7
    rCSI 10.1%
    PRS 94.1%
  • small intestine goblet cell CL1000495
    CSI 2.7
    rCSI 6.0%
    PRS 97.8%
  • OFF midget ganglion cell CL4033047
    CSI 2.9
    rCSI 58.5%
    PRS 92.7%
  • ON midget ganglion cell CL4033046
    CSI 2.9
    rCSI 58.9%
    PRS 92.6%
  • basket cell CL0000118
    CSI 2.9
    rCSI 18.2%
    PRS 85.6%
  • retinal rod cell CL0000604
    CSI 2.9
    rCSI 5.2%
    PRS 95.0%
  • GABAergic interneuron CL0011005
    CSI 2.9
    rCSI 46.4%
    PRS 96.8%
  • neural progenitor cell CL0011020
    CSI 3.0
    rCSI 13.2%
    PRS 91.0%
  • neuroendocrine cell CL0000165
    CSI 3.1
    rCSI 11.8%
    PRS 97.5%
  • mesenchymal stem cell of adipose tissue CL0002570
    CSI 3.1
    rCSI 17.2%
    PRS 98.2%
  • cerebral cortex pyramidal neuron CL4023111
    CSI 3.2
    rCSI 19.6%
    PRS 96.6%
  • suprabasal keratinocyte CL4033013
    CSI 3.3
    rCSI 5.4%
    PRS 79.9%
  • adventitial cell CL0002503
    CSI 3.4
    rCSI 8.2%
    PRS 98.4%
  • ON-bipolar cell CL0000749
    CSI 3.6
    rCSI 5.3%
    PRS 96.2%
  • OFFx cell CL4033036
    CSI 3.6
    rCSI 17.2%
    PRS 90.6%
  • midbrain dopaminergic neuron CL2000097
    CSI 3.7
    rCSI 23.6%
    PRS 93.3%
  • brain vascular cell CL4023072
    CSI 3.7
    rCSI 38.6%
    PRS 93.4%
  • stromal cell of ovary CL0002132
    CSI 3.8
    rCSI 10.5%
    PRS 98.4%
  • cerebellar neuron CL1001611
    CSI 3.9
    rCSI 33.9%
    PRS 90.1%
  • forebrain radial glial cell CL0013000
    CSI 3.9
    rCSI 12.6%
    PRS 97.4%
  • melanocyte CL0000148
    CSI 4.1
    rCSI 3.0%
    PRS 96.1%
  • differentiation-committed oligodendrocyte precursor CL4023059
    CSI 4.3
    rCSI 7.7%
    PRS 94.3%
  • retinal cone cell CL0000573
    CSI 4.3
    rCSI 6.9%
    PRS 93.4%
  • ON parasol ganglion cell CL4033052
    CSI 4.3
    rCSI 61.4%
    PRS 92.9%
  • starburst amacrine cell CL0004232
    CSI 4.6
    rCSI 38.9%
    PRS 88.9%
  • kidney loop of Henle thick ascending limb epithelial cell CL1001106
    CSI 4.7
    rCSI 40.5%
    PRS 94.3%
  • endothelial cell of vascular tree CL0002139
    CSI 4.7
    rCSI 25.8%
    PRS 95.3%
  • melanocyte of skin CL1000458
    CSI 5.1
    rCSI 6.9%
    PRS 79.4%
  • regular atrial cardiac myocyte CL0002129
    CSI 5.3
    rCSI 16.9%
    PRS 95.0%
  • stem cell CL0000034
    CSI 5.4
    rCSI 5.2%
    PRS 96.3%
  • stromal cell CL0000499
    CSI 5.4
    rCSI 15.2%
    PRS 96.2%
  • diffuse bipolar 4 cell CL4033031
    CSI 5.7
    rCSI 64.8%
    PRS 88.1%
  • mesodermal cell CL0000222
    CSI 5.7
    rCSI 6.9%
    PRS 97.9%
  • kidney interstitial fibroblast CL1000692
    CSI 5.8
    rCSI 30.7%
    PRS 94.6%
  • cardiac blood vessel endothelial cell CL0010006
    CSI 5.8
    rCSI 41.0%
    PRS 93.9%
  • mesangial cell CL0000650
    CSI 5.9
    rCSI 24.1%
    PRS 98.6%
  • conjunctival epithelial cell CL1000432
    CSI 6.2
    rCSI 9.5%
    PRS 96.7%
  • diffuse bipolar 1 cell CL4033027
    CSI 6.2
    rCSI 46.9%
    PRS 90.5%
  • basal cell of epidermis CL0002187
    CSI 6.6
    rCSI 11.7%
    PRS 78.3%
  • podocyte CL0000653
    CSI 6.6
    rCSI 29.5%
    PRS 97.5%
  • medium spiny neuron CL1001474
    CSI 6.8
    rCSI 58.3%
    PRS 93.3%
  • retina horizontal cell CL0000745
    CSI 6.8
    rCSI 10.4%
    PRS 95.8%
  • pancreatic A cell CL0000171
    CSI 7.0
    rCSI 7.4%
    PRS 97.8%
  • chondrocyte CL0000138
    CSI 7.1
    rCSI 11.4%
    PRS 95.5%
  • kidney connecting tubule epithelial cell CL1000768
    CSI 7.4
    rCSI 18.8%
    PRS 95.2%
  • mature astrocyte CL0002627
    CSI 7.5
    rCSI 32.0%
    PRS 94.4%
  • central nervous system neuron CL2000029
    CSI 7.8
    rCSI 57.1%
    PRS 93.7%
  • kidney interstitial alternatively activated macrophage CL1000695
    CSI 7.8
    rCSI 20.3%
    PRS 98.4%
  • erythroblast CL0000765
    CSI 7.8
    rCSI 20.7%
    PRS 97.1%
  • mesenchymal cell CL0008019
    CSI 8.0
    rCSI 20.2%
    PRS 96.2%
  • contractile cell CL0000183
    CSI 8.1
    rCSI 24.0%
    PRS 97.6%
  • tracheobronchial smooth muscle cell CL0019019
    CSI 8.2
    rCSI 14.5%
    PRS 98.2%
  • astrocyte of the cerebral cortex CL0002605
    CSI 8.7
    rCSI 19.5%
    PRS 92.7%
  • basal cell CL0000646
    CSI 9.0
    rCSI 12.0%
    PRS 96.1%
  • H2 horizontal cell CL0004218
    CSI 9.2
    rCSI 45.5%
    PRS 94.0%
  • cardiac muscle cell CL0000746
    CSI 9.2
    rCSI 13.2%
    PRS 93.2%
  • retinal pigment epithelial cell CL0002586
    CSI 9.4
    rCSI 18.7%
    PRS 95.7%
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 9.5
    rCSI 56.1%
    PRS 92.4%
  • cerebellar granule cell CL0001031
    CSI 10.3
    rCSI 15.2%
    PRS 94.8%
  • dopaminergic neuron CL0000700
    CSI 10.4
    rCSI 58.5%
    PRS 91.8%
  • macroglial cell CL0000126
    CSI 10.4
    rCSI 26.6%
    PRS 95.3%
  • diffuse bipolar 6 cell CL4033032
    CSI 10.6
    rCSI 55.7%
    PRS 91.2%
  • neural cell CL0002319
    CSI 10.8
    rCSI 40.8%
    PRS 89.9%
  • Mueller cell CL0000636
    CSI 10.9
    rCSI 25.0%
    PRS 94.6%
  • cerebral cortex GABAergic interneuron CL0010011
    CSI 11.0
    rCSI 32.4%
    PRS 97.1%
  • Bergmann glial cell CL0000644
    CSI 11.2
    rCSI 15.3%
    PRS 93.8%
  • invaginating midget bipolar cell CL4033034
    CSI 11.2
    rCSI 65.9%
    PRS 91.7%
  • amacrine cell CL0000561
    CSI 11.3
    rCSI 32.7%
    PRS 93.3%
  • progenitor cell CL0011026
    CSI 11.4
    rCSI 24.2%
    PRS 94.0%
  • kidney collecting duct principal cell CL1001431
    CSI 11.8
    rCSI 59.5%
    PRS 95.5%
  • cerebral cortex neuron CL0010012
    CSI 11.9
    rCSI 48.5%
    PRS 92.9%
  • vascular associated smooth muscle cell CL0000359
    CSI 12.0
    rCSI 39.0%
    PRS 97.2%
  • hepatic stellate cell CL0000632
    CSI 12.3
    rCSI 45.9%
    PRS 96.3%
  • glioblast CL0000030
    CSI 12.3
    rCSI 19.6%
    PRS 94.0%
  • neural crest cell CL0011012
    CSI 12.3
    rCSI 9.8%
    PRS 95.5%
  • radial glial cell CL0000681
    CSI 12.5
    rCSI 17.4%
    PRS 97.1%
  • serotonergic neuron CL0000850
    CSI 12.7
    rCSI 56.6%
    PRS 88.5%
  • H1 horizontal cell CL0004217
    CSI 12.7
    rCSI 50.3%
    PRS 93.6%
  • blood vessel endothelial cell CL0000071
    CSI 12.9
    rCSI 26.7%
    PRS 96.8%
  • retinal ganglion cell CL0000740
    CSI 13.5
    rCSI 29.7%
    PRS 92.4%
  • parietal epithelial cell CL1000452
    CSI 13.8
    rCSI 36.9%
    PRS 95.7%
  • renal interstitial pericyte CL1001318
    CSI 13.8
    rCSI 38.1%
    PRS 96.9%
  • GABAergic neuron CL0000617
    CSI 13.9
    rCSI 46.6%
    PRS 90.0%
  • alveolar adventitial fibroblast CL4028006
    CSI 14.0
    rCSI 22.1%
    PRS 98.2%
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 14.2
    rCSI 53.6%
    PRS 92.2%
  • glutamatergic neuron CL0000679
    CSI 14.6
    rCSI 29.9%
    PRS 90.5%
  • alveolar type 1 fibroblast cell CL4028004
    CSI 14.7
    rCSI 16.1%
    PRS 98.3%
  • GABAergic amacrine cell CL4030027
    CSI 14.8
    rCSI 50.8%
    PRS 90.4%

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.

Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ROBO2](/details-gene/6092) (Roundabout Guidance Receptor 2) is a protein-coding gene located on chromosome 3p12.3. It encodes a transmembrane receptor that is a member of the evolutionarily conserved Roundabout (Robo) family. The primary function of [ROBO2](/details-gene/6092) is to act as a receptor for Slit guidance ligands, mediating repulsive signals that are critical for axon guidance during the development of the central nervous system. Consistent with this role, expression data from the **Overall** context shows high significance in numerous neuronal subtypes, particularly diverse classes of cortical interneurons. Beyond its role in neurodevelopment, functional annotations and clinical data link [ROBO2](/details-gene/6092) to the development of other organ systems, such as the kidneys and heart. Mutations in this gene are associated with congenital anomalies of the kidney and urinary tract (CAKUT), including vesicoureteral reflux ([OMIM: 602431](https://omim.org/entry/602431)). ## Cellular Roles and Expression Landscape The expression profile of [ROBO2](/details-gene/6092) strongly indicates a specialized role in the developing and mature nervous system. In the **Overall** context, it is a highly significant marker for a wide array of neuronal cell types. Its most prominent expression is observed in various subtypes of inhibitory interneurons in the cerebral cortex, including [VIP GABAergic cortical interneuron](/details-cell/CL4023016) (CSI: 53.55), [sst GABAergic cortical interneuron](/details-cell/CL4023017) (CSI: 49.26), and [pvalb GABAergic cortical interneuron](/details-cell/CL4023018) (CSI: 47.82). This suggests a critical function in establishing the complex inhibitory circuitry that governs cortical information processing. The high significance in [caudal ganglionic eminence derived cortical interneuron](/details-cell/CL4023064) and progenitor cells like [neuroblast (sensu Vertebrata)](/details-cell/CL0000031) highlights its involvement from early stages of neuronal migration and differentiation. Furthermore, [ROBO2](/details-gene/6092) is also significantly expressed in excitatory neurons, such as [L2/3 intratelencephalic projecting glutamatergic neuron](/details-cell/CL4030059), and other neural populations like [retinal bipolar neuron](/details-cell/CL0000748). This broad yet specific expression pattern across diverse neuronal classes underscores its fundamental importance in wiring multiple components of the central nervous system. ## Pathways and Molecular Function The functions of [ROBO2](/details-gene/6092) are well-annotated in several key biological pathways, primarily centered on developmental processes. As a cell surface protein located on the [GO:0005886](https://www.ebi.ac.uk/QuickGO/term/GO:0005886) (plasma membrane) and specifically the [GO:0030673](https://www.ebi.ac.uk/QuickGO/term/GO:0030673) (axolemma), its principal molecular function is '[GO:0008046](https://www.ebi.ac.uk/QuickGO/term/GO:0008046)' (axon guidance receptor activity). This activity is central to the Reactome pathway '[R-HSA-422475](https://reactome.org/content/detail/R-HSA-422475)' (Axon guidance). The canonical mechanism involves the binding of Slit family proteins to the [ROBO2](/details-gene/6092) receptor, which triggers intracellular signaling cascades that repel the extending axon from the midline and other inappropriate targets [Link](https://doi.org/10.1016/s0092-8674(00)80590-5). This process is crucial for establishing the precise contralateral projections that define nervous system architecture [Link](https://doi.org/10.1016/s0092-8674(00)80915-0). Beyond the nervous system, functional annotations reveal a significant role in organogenesis. GO terms such as '[GO:0001656](https://www.ebi.ac.uk/QuickGO/term/GO:0001656)' (Metanephros development) and '[GO:0001657](https://www.ebi.ac.uk/QuickGO/term/GO:0001657)' (Ureteric bud development), along with the Reactome pathway '[R-HSA-9830369](https://reactome.org/content/detail/R-HSA-9830369)' (Kidney development), are highly consistent with the clinical observation that disruption of [ROBO2](/details-gene/6092) can lead to urinary tract anomalies [Link](https://doi.org/10.1086/512735). This suggests that the Slit-Robo signaling axis is a conserved mechanism used for cell guidance and tissue morphogenesis in multiple developmental contexts. ## Research Directions The well-defined role of [ROBO2](/details-gene/6092) in axon guidance and its specific expression pattern provide a strong foundation for further investigation into its precise functions in neural circuit formation and developmental disorders. ### Proposed Hypotheses: 1. **Subtype-Specific Interneuron Wiring:** Given the high significance of [ROBO2](/details-gene/6092) across multiple distinct cortical interneuron subtypes ([VIP](/details-cell/CL4023016), [sst](/details-cell/CL4023017), [pvalb](/details-cell/CL4023018)), we hypothesize that [ROBO2](/details-gene/6092) signaling is not just a general guidance cue, but is differentially interpreted or modulated in these subtypes to control their specific laminar positioning, dendritic arborization, and synaptic targeting onto pyramidal neurons. 2. **Role in Synaptic Plasticity:** Beyond its developmental role, we hypothesize that [ROBO2](/details-gene/6092) expression in mature neurons contributes to synaptic maintenance or plasticity. Its regulation may be a mechanism to permit or restrict structural changes at the synapse in the adult brain, and its misregulation could contribute to synaptic deficits in neurological disorders. ### Key Experimental Approach: To test the first hypothesis regarding subtype-specific interneuron wiring, a powerful approach would involve conditional gene knockout in mice. By crossing a `Robo2-floxed` mouse line with various Cre-driver lines that target specific interneuron precursors (e.g., `Nkx2.1-Cre`) or mature subtypes (e.g., `Sst-Cre`, `Vip-Cre`), one could selectively ablate [ROBO2](/details-gene/6092) function. The consequences could be assessed using a combination of advanced imaging (to analyze interneuron distribution and morphology in the cortex), whole-cell patch-clamp electrophysiology (to measure changes in inhibitory synaptic transmission), and circuit mapping techniques to determine if the loss of [ROBO2](/details-gene/6092) leads to predictable wiring errors specific to the targeted interneuron population. ### Therapeutic Potential: The primary clinical relevance of [ROBO2](/details-gene/6092) relates to congenital developmental disorders, making it a key gene for diagnostic screening rather than a direct therapeutic target for these conditions. However, as a cell surface receptor, it is theoretically druggable. In contexts of nerve injury or neurodegenerative disease, where controlled axonal regeneration is desired, modulation of the Slit-Robo signaling pathway could be a viable strategy. Activating [ROBO2](/details-gene/6092) signaling or providing engineered ligands might help guide regenerating axons. Conversely, inhibiting its function could be explored in cancers where developmental pathways are reactivated to promote cell migration and metastasis. However, any such intervention would require extreme precision to avoid disrupting the stable architecture of the adult nervous system.

Genular Protein ID: 3926584561

Symbol: ROBO2_HUMAN

Name: Roundabout homolog 2

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 16829019

Title: Isolation and differential expression of two isoforms of the ROBO2/Robo2 axon guidance receptor gene in humans and mice.

PubMed ID: 16829019

DOI: 10.1016/j.ygeno.2006.05.011

PubMed ID: 10997877

Title: Prediction of the coding sequences of unidentified human genes. XVIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.

PubMed ID: 10997877

DOI: 10.1093/dnares/7.4.271

PubMed ID: 16641997

Title: The DNA sequence, annotation and analysis of human chromosome 3.

PubMed ID: 16641997

DOI: 10.1038/nature04728

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 9458045

Title: Roundabout controls axon crossing of the CNS midline and defines a novel subfamily of evolutionarily conserved guidance receptors.

PubMed ID: 9458045

DOI: 10.1016/s0092-8674(00)80915-0

PubMed ID: 10102268

Title: Slit proteins bind Robo receptors and have an evolutionarily conserved role in repulsive axon guidance.

PubMed ID: 10102268

DOI: 10.1016/s0092-8674(00)80590-5

PubMed ID: 11404413

Title: Diversity and specificity of actions of Slit2 proteolytic fragments in axon guidance.

PubMed ID: 11404413

DOI: 10.1523/jneurosci.21-12-04281.2001

PubMed ID: 17357069

Title: Disruption of ROBO2 is associated with urinary tract anomalies and confers risk of vesicoureteral reflux.

PubMed ID: 17357069

DOI: 10.1086/512735

Sequence Information:

  • Length: 1378
  • Mass: 151200
  • Checksum: 60F7CE3E53622B50
  • Sequence:
  • MSLLMFTQLL LCGFLYVRVD GSRLRQEDFP PRIVEHPSDV IVSKGEPTTL NCKAEGRPTP 
    TIEWYKDGER VETDKDDPRS HRMLLPSGSL FFLRIVHGRR SKPDEGSYVC VARNYLGEAV 
    SRNASLEVAL LRDDFRQNPT DVVVAAGEPA ILECQPPRGH PEPTIYWKKD KVRIDDKEER 
    ISIRGGKLMI SNTRKSDAGM YTCVGTNMVG ERDSDPAELT VFERPTFLRR PINQVVLEEE 
    AVEFRCQVQG DPQPTVRWKK DDADLPRGRY DIKDDYTLRI KKTMSTDEGT YMCIAENRVG 
    KMEASATLTV RAPPQFVVRP RDQIVAQGRT VTFPCETKGN PQPAVFWQKE GSQNLLFPNQ 
    PQQPNSRCSV SPTGDLTITN IQRSDAGYYI CQALTVAGSI LAKAQLEVTD VLTDRPPPII 
    LQGPANQTLA VDGTALLKCK ATGDPLPVIS WLKEGFTFPG RDPRATIQEQ GTLQIKNLRI 
    SDTGTYTCVA TSSSGETSWS AVLDVTESGA TISKNYDLSD LPGPPSKPQV TDVTKNSVTL 
    SWQPGTPGTL PASAYIIEAF SQSVSNSWQT VANHVKTTLY TVRGLRPNTI YLFMVRAINP 
    QGLSDPSPMS DPVRTQDISP PAQGVDHRQV QKELGDVLVR LHNPVVLTPT TVQVTWTVDR 
    QPQFIQGYRV MYRQTSGLQA TSSWQNLDAK VPTERSAVLV NLKKGVTYEI KVRPYFNEFQ 
    GMDSESKTVR TTEEAPSAPP QSVTVLTVGS YNSTSISVSW DPPPPDHQNG IIQEYKIWCL 
    GNETRFHINK TVDAAIRSVI IGGLFPGIQY RVEVAASTSA GVGVKSEPQP IIIGRRNEVV 
    ITENNNSITE QITDVVKQPA FIAGIGGACW VILMGFSIWL YWRRKKRKGL SNYAVTFQRG 
    DGGLMSNGSR PGLLNAGDPS YPWLADSWPA TSLPVNNSNS GPNEIGNFGR GDVLPPVPGQ 
    GDKTATMLSD GAIYSSIDFT TKTSYNSSSQ ITQATPYATT QILHSNSIHE LAVDLPDPQW 
    KSSIQQKTDL MGFGYSLPDQ NKGNNGGKGG KKKKNKNSSK PQKNNGSTWA NVPLPPPPVQ 
    PLPGTELEHY AVEQQENGYD SDSWCPPLPV QTYLHQGLED ELEEDDDRVP TPPVRGVASS 
    PAISFGQQST ATLTPSPREE MQPMLQAHLD ELTRAYQFDI AKQTWHIQSN NQPPQPPVPP 
    LGYVSGALIS DLETDVADDD ADDEEEALEI PRPLRALDQT PGSSMDNLDS SVTGKAFTSS 
    QRPRPTSPFS TDSNTSAALS QSQRPRPTKK HKGGRMDQQP ALPHRREGMT DEEALVPYSK 
    PSFPSPGGHS SSGTASSKGS TGPRKTEVLR AGHQRNASDL LDIGYMGSNS QGQFTGEL

Genular Protein ID: 1556530623

Symbol: F8W703_HUMAN

Name: N/A

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 11237011

Title: Initial sequencing and analysis of the human genome.

PubMed ID: 11237011

DOI: 10.1038/35057062

PubMed ID: 15496913

Title: Finishing the euchromatic sequence of the human genome.

PubMed ID: 15496913

DOI: 10.1038/nature03001

PubMed ID: 16641997

Title: The DNA sequence, annotation and analysis of human chromosome 3.

PubMed ID: 16641997

DOI: 10.1038/nature04728

Sequence Information:

  • Length: 1443
  • Mass: 157778
  • Checksum: 0ACB7BB7E42D4234
  • Sequence:
  • MSLLMFTQLL LCGFLYVRVD GSRLRQEDFP PRIVEHPSDV IVSKGEPTTL NCKAEGRPTP 
    TIEWYKDGER VETDKDDPRS HRMLLPSGSL FFLRIVHGRR SKPDEGSYVC VARNYLGEAV 
    SRNASLEVAL LRDDFRQNPT DVVVAAGEPA ILECQPPRGH PEPTIYWKKD KVRIDDKEER 
    ISIRGGKLMI SNTRKSDAGM YTCVGTNMVG ERDSDPAELT VFERPTFLRR PINQVVLEEE 
    AVEFRCQVQG DPQPTVRWKK DDADLPRGRY DIKDDYTLRI KKTMSTDEGT YMCIAENRVG 
    KMEASATLTV RARPVAPPQF VVRPRDQIVA QGRTVTFPCE TKGNPQPAVF WQKEGSQNLL 
    FPNQPQQPNS RCSVSPTGDL TITNIQRSDA GYYICQALTV AGSILAKAQL EVTDVLTDRP 
    PPIILQGPAN QTLAVDGTAL LKCKATGDPL PVISWLKEGF TFPGRDPRAT IQEQGTLQIK 
    NLRISDTGTY TCVATSSSGE TSWSAVLDVT ESGATISKNY DLSDLPGPPS KPQVTDVTKN 
    SVTLSWQPGT PGTLPASAYI IEAFSQSVSN SWQTVANHVK TTLYTVRGLR PNTIYLFMVR 
    AINPQGLSDP SPMSDPVRTQ DISPPAQGVD HRQVQKELGD VLVRLHNPVV LTPTTVQVTW 
    TVDRQPQFIQ GYRVMYRQTS GLQATSSWQN LDAKVPTERS AVLVNLKKGV TYEIKVRPYF 
    NEFQGMDSES KTVRTTEEAP SAPPQSVTVL TVGSYNSTSI SVSWDPPPPD HQNGIIQEYK 
    IWCLGNETRF HINKTVDAAI RSVIIGGLFP GIQYRVEVAA STSAGVGVKS EPQPIIIGRR 
    NEVVITENNN SITEQITDVV KQPAFIAGIG GACWVILMGF SIWLYWRRKK RKGLSNYAVT 
    FQRGDGGLMS NGSRPGLLNA GDPSYPWLAD SWPATSLPVN NSNSGPNEIG NFGRGDVLPP 
    VPGQGDKTAT MLSDGAIYSS IDFTTKTSYN SSSQITQATP YATTQILHSN SIHELAVDLP 
    DPQWKSSIQQ KTDLMGFGYS LPDQNKGNNG GKGGKKKKNK NSSKPQKNNG STWANVPLPP 
    PPVQPLPGTE LEHYAVEQQE NGYDSDSWCP PLPVQTYLHQ GLEDELEEDD DRVPTPPVRG 
    VASSPAISFG QQSTATLTPS PREEMQPMLQ AHLDELTRAY QFDIAKQTWH IQSNNQPPQP 
    PVPPLGYVSG ALISDLETDV ADDDADDEEE ALEIPRPLRA LDQTPGSSMD NLDSSVTGSM 
    VNGWGSASDE DRNFSSHRSS VGSSSDGSIF ASGSFAQALV AAADKAGFRL DGTSLTRTGK 
    AFTSSQRPRP TSPFSTDSNT SAALSQSQRP RPTKKHKGGR MDQQPALPHR REGMTDEEAL 
    VPYSKPSFPS PGGHSSSGTA SSKGSTGPRK TEVLRAGHQR NASDLLDIGY MGSNSQGQFT 
    GEL