Details for: ZNF189

Gene ID: 7743

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF189

Ensembl ID: ENSG00000136870

Description: zinc finger protein 189

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 4.55
    rCSI 16.36%
    PRS 74.7
  • Kupffer cell CL0000091
    CSI 3.05
    rCSI 6.99%
    PRS 90.96
  • pancreatic A cell CL0000171
    CSI 2.61
    rCSI 2.73%
    PRS 91.81
  • hepatocyte CL0000182
    CSI 2.5
    rCSI 4.47%
    PRS 88.75
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 2.43
    rCSI 5.9%
    PRS 74.57
  • hepatic stellate cell CL0000632
    CSI 2.39
    rCSI 8.94%
    PRS 85.17
  • blood vessel endothelial cell CL0000071
    CSI 2.34
    rCSI 4.85%
    PRS 88.21
  • myofibroblast cell CL0000186
    CSI 2.34
    rCSI 3.24%
    PRS 86.77
  • intrahepatic cholangiocyte CL0002538
    CSI 2.17
    rCSI 5.22%
    PRS 90.23
  • choroid plexus epithelial cell CL0000706
    CSI 2.16
    rCSI 3.53%
    PRS 82.72
  • centrilobular region hepatocyte CL0019029
    CSI 2.08
    rCSI 5.44%
    PRS 86.11
  • BEST4+ enteroycte CL4030026
    CSI 2.07
    rCSI 2.57%
    PRS 89.82
  • intestinal epithelial cell CL0002563
    CSI 2.04
    rCSI 2.13%
    PRS 87.59
  • cardiac muscle cell CL0000746
    CSI 2
    rCSI 2.86%
    PRS 82.15
  • retinal pigment epithelial cell CL0002586
    CSI 1.98
    rCSI 3.94%
    PRS 86.54
  • pancreatic acinar cell CL0002064
    CSI 1.94
    rCSI 2.58%
    PRS 92.82
  • cardiac endothelial cell CL0010008
    CSI 1.85
    rCSI 7.46%
    PRS 90.58
  • stem cell CL0000034
    CSI 1.72
    rCSI 1.66%
    PRS 85.89
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 1.57
    rCSI 1.96%
    PRS 74.58
  • direct pathway medium spiny neuron CL4023026
    CSI 1.57
    rCSI 37.48%
    PRS 74.39
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 1.54
    rCSI 2.59%
    PRS 76.77
  • regular atrial cardiac myocyte CL0002129
    CSI 1.54
    rCSI 4.95%
    PRS 86.56
  • indirect pathway medium spiny neuron CL4023029
    CSI 1.45
    rCSI 34.92%
    PRS 74.57
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 1.36
    rCSI 2.4%
    PRS 76.22
  • parietal epithelial cell CL1000452
    CSI 1.28
    rCSI 3.42%
    PRS 84.42
  • cholangiocyte CL1000488
    CSI 1.2
    rCSI 7.2%
    PRS 89.36
  • regular ventricular cardiac myocyte CL0002131
    CSI 0.93
    rCSI 5.8%
    PRS 83.74
  • L6b glutamatergic cortical neuron CL4023038
    CSI 0.72
    rCSI 2.26%
    PRS 78.17
  • podocyte CL0000653
    CSI 0.67
    rCSI 2.99%
    PRS 90.53
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 0.6
    rCSI 3.54%
    PRS 77.24

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF189](/details-gene/7743) is a protein-coding gene located on chromosome 9q31.1 that encodes Zinc finger protein 189. Functional annotations strongly indicate that [ZNF189](/details-gene/7743) acts as a DNA-binding transcription factor specific to RNA polymerase II, playing a role in the regulation of gene expression. Expression data highlights its significance in a diverse array of highly specialized, terminally differentiated cells, including specific neuronal subtypes such as the [L5 extratelencephalic projecting glutamatergic cortical neuron](/details-cell/CL4023041), as well as key hepatic cells like the [Kupffer cell](/details-cell/CL0000091) and [hepatocyte](/details-cell/CL0000182), suggesting a role in maintaining the unique transcriptional identity of these distinct cell types. ## Cellular Roles and Expression Landscape **Overall**, the expression profile of [ZNF189](/details-gene/7743) suggests a critical function in maintaining the state of specialized cells across various tissues, rather than a ubiquitous housekeeping role. Its highest cell significance index (CSI) is observed in the [L5 extratelencephalic projecting glutamatergic cortical neuron](/details-cell/CL4023041) (CSI: 4.55), pointing towards a potentially crucial role in the identity and function of this specific neuronal population in the cerebral cortex. The gene also demonstrates significant expression across multiple cell types within the liver microenvironment. These include immune-resident [Kupffer cells](/details-cell/CL0000091) (CSI: 3.05), parenchymal [hepatocytes](/details-cell/CL0000182) (CSI: 2.50), [hepatic stellate cells](/details-cell/CL0000632) (CSI: 2.39), and [intrahepatic cholangiocytes](/details-cell/CL0002538) (CSI: 2.17). This widespread significance within a single organ suggests [ZNF189](/details-gene/7743) may be involved in coordinating transcriptional programs that support liver homeostasis. Additionally, notable expression is seen in other diverse cell types, including [pancreatic A cells](/details-cell/CL0000171), [blood vessel endothelial cells](/details-cell/CL0000071), and [cardiac muscle cells](/details-cell/CL0000746), reinforcing its importance in a variety of specialized biological contexts. ## Pathways and Molecular Function The functional annotations for [ZNF189](/details-gene/7743) are highly consistent with its proposed role as a transcriptional regulator. It is primarily localized to the [nucleus](/details-cell/GO:0005634) and is involved in fundamental transcriptional processes. * **Biological Process:** Its principal annotated function is the [regulation of transcription by rna polymerase ii](/details-cell/GO:0006357), which is the core process for expressing protein-coding genes. * **Molecular Function:** It possesses [dna-binding transcription factor activity, rna polymerase ii-specific](/details-cell/GO:0000981) and [rna polymerase ii cis-regulatory region sequence-specific dna binding](/details-cell/GO:0000978). This indicates that it recognizes and binds to specific DNA sequences to control the expression of target genes. The annotation for [metal ion binding](/details-cell/GO:0046872) is consistent with its identity as a zinc finger protein, as these domains require zinc ions for their structural integrity and DNA-binding capability. * **Reactome Pathways:** Its involvement in pathways such as [Gene expression (transcription)](https://reactome.org/content/detail/R-HSA-74160) and [Rna polymerase ii transcription](https://reactome.org/content/detail/R-HSA-73857) further solidifies its role as a key component of the cell's core machinery for gene expression. This function is likely leveraged to establish and maintain the specialized transcriptomes of the cell types where it is highly expressed, such as neurons and hepatocytes. ## Research Directions The specific and high expression of [ZNF189](/details-gene/7743) in terminally differentiated cells provides a foundation for several testable hypotheses regarding its precise biological roles. 1. **Hypothesis 1:** Given its top ranking in [L5 extratelencephalic projecting glutamatergic cortical neurons](/details-cell/CL4023041), [ZNF189](/details-gene/7743) may function as a master regulator essential for the establishment or long-term maintenance of the transcriptional identity and synaptic function of this specific neuronal subtype. Its dysregulation could contribute to neurological disorders characterized by cortical dysfunction. 2. **Hypothesis 2:** The significant expression of [ZNF189](/details-gene/7743) across multiple distinct liver cell types ([hepatocytes](/details-cell/CL0000182), [Kupffer cells](/details-cell/CL0000091), [stellate cells](/details-cell/CL0000632)) suggests it coordinates a shared transcriptional network required for intercellular communication and metabolic homeostasis in the liver. Its disruption could impair liver function or contribute to the pathogenesis of liver diseases like fibrosis or non-alcoholic steatohepatitis (NASH). **Proposed Experiment:** To test Hypothesis 1, a conditional knockout of *ZNF189* in a mouse model would be highly informative. Using a Cre-driver line specific to layer 5 cortical neurons (e.g., *Fezf2-CreER*), the gene could be ablated at a specific developmental or adult time point. The consequences would be assessed using single-cell RNA sequencing to identify changes in the neuronal transcriptome, combined with electrophysiological recordings (patch-clamp) to measure functional deficits in synaptic transmission and neuronal excitability. **Therapeutic Potential:** As an intracellular, nucleus-localized transcription factor, [ZNF189](/details-gene/7743) presents a challenging direct target for conventional small molecule inhibitors or antibody-based therapies. However, if its downstream target genes or the signaling pathways it regulates are identified, those components might offer more tractable therapeutic entry points. Furthermore, its high specificity in certain cell types could make it a valuable biomarker for monitoring the health or disease state of those cells, particularly in the context of neurological or hepatic disorders.

Genular Protein ID: 1907960007

Symbol: ZN189_HUMAN

Name: Zinc finger protein 189

UniProtKB Accession Codes:

O75820 O75802 Q5T7D7 Q5T7D8 Q5T7D9 Q9UBL4 Q9UPE9 Q9UPF0 Q9UPF1

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 3 CDD 1 CTD 1 DNASU 1 DisGeNET 1 EMBL 13 Ensembl 3 ExpressionAtlas 1 GO 5 Gene3D 2 GeneCards 1 GeneTree 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 5 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 4 Pumba 1 RNAct 1 Reactome 1 RefSeq 9 SMART 2 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 9653648

Title: Cloning and characterization of ZNF189, a novel human Kruppel-like zinc finger gene localized to chromosome 9q22-q31.

PubMed ID: 9653648

DOI: 10.1006/geno.1998.5309

PubMed ID: 10415338

Title: Context-dependent Taq-polymerase-mediated nucleotide alterations, as revealed by direct sequencing of the ZNF189 gene: implications for mutation detection.

PubMed ID: 10415338

DOI: 10.1016/s0378-1119(99)00205-x

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15164053

Title: DNA sequence and analysis of human chromosome 9.

PubMed ID: 15164053

DOI: 10.1038/nature02465

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 25218447

Title: Uncovering global SUMOylation signaling networks in a site-specific manner.

PubMed ID: 25218447

DOI: 10.1038/nsmb.2890

PubMed ID: 25772364

Title: SUMO-2 orchestrates chromatin modifiers in response to DNA damage.

PubMed ID: 25772364

DOI: 10.1016/j.celrep.2015.02.033

PubMed ID: 25755297

Title: System-wide analysis of SUMOylation dynamics in response to replication stress reveals novel small ubiquitin-like modified target proteins and acceptor lysines relevant for genome stability.

PubMed ID: 25755297

DOI: 10.1074/mcp.o114.044792

PubMed ID: 28112733

Title: Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.

PubMed ID: 28112733

DOI: 10.1038/nsmb.3366

Sequence Information:

  • Length: 626
  • Mass: 72976
  • Checksum: BF6E0F9943F15E4C
  • Sequence:
    • MASPSPPPES KGLLTFEDVA VFFTQEEWDY LDPAQRSLYK DVMMENYGNL VSLDVLNRDK 
DEEPTVKQEI EEIEEEVEPQ GVIVTRIKSE IDQDPMGRET FELVGRLDKQ RGIFLWEIPR 
ESLTQEQRMF RENTNIIRKR PNSEEKCHKC EECGKGFVRK AHFIQHQRVH TGEKPFQCNE 
CGKSFSRSSF VIEHQRIHTG ERPYECNYCG KTFSVSSTLI RHQRIHTGER PYQCNQCKQS 
FSQRRSLVKH QRIHTGEKPH KCSDCGKAFS WKSHLIEHQR THTGEKPYHC TKCKKSFSRN 
SLLVEHQRIH TGERPHKCGE CGKAFRLSTY LIQHQKIHTG EKPFLCIECG KSFSRSSFLI 
EHQRIHTGER PYQCKECGKS FSQLCNLTRH QRIHTGDKPH KCEECGKAFS RSSGLIQHQR 
IHTREKTYPY NETKESFDPN CSLVIQQEVY PKEKSYKCDE CGKTFSVSAH LVQHQRIHTG 
EKPYLCTVCG KSFSRSSFLI EHQRIHTGER PYLCRQCGKS FSQLCNLIRH QGVHTGNKPH 
KCDECGKAFS RNSGLIQHQR IHTGEKPYKC EKCDKSFSQQ RSLVNHQKIH AEVKTQETHE 
CDACGEAFNC RISLIQHQKL HTAWMQ

Genular Protein ID: 2834236794

Symbol: A0A087X0K2_HUMAN

Name: N/A

UniProtKB Accession Codes:

A0A087X0K2

Database IDs:

AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID-ORCS 1 CDD 1 CTD 1 DNASU 1 DisGeNET 1 EMBL 1 Ensembl 2 ExpressionAtlas 1 GO 1 Gene3D 1 GeneTree 1 HGNC 1 HOGENOM 1 InterPro 3 MassIVE 1 NCBI Taxonomy 1 OrthoDB 1 PANTHER 2 PROSITE 2 PeptideAtlas 2 Pfam 1 Proteomes 2 ProteomicsDB 1 RefSeq 1 SMART 1 SMR 1 SUPFAM 1 UCSC 1 VEuPathDB 1

Citations:

PubMed ID: 11237011

Title: Initial sequencing and analysis of the human genome.

PubMed ID: 11237011

DOI: 10.1038/35057062

PubMed ID: 15164053

Title: DNA sequence and analysis of human chromosome 9.

PubMed ID: 15164053

DOI: 10.1038/nature02465

PubMed ID: 15496913

Title: Finishing the euchromatic sequence of the human genome.

PubMed ID: 15496913

DOI: 10.1038/nature03001

Sequence Information:

  • Length: 85
  • Mass: 9609
  • Checksum: D31D9B3558B8FD26
  • Sequence:
    • MASPSPPPES KEEWDYLDPA QRSLYKDVMM ENYGNLVSLG LTLLPRLVCS DANTACCSLN 
LLGLSDQSSH LSYLSSWDYR CFEQR

Genular Protein ID: 1874173285

Symbol: B7ZLK9_HUMAN

Name: N/A

UniProtKB Accession Codes:

B7ZLK9

Database IDs:

ARBA 4 AlphaFoldDB 1 BioGRID-ORCS 1 CDD 1 CTD 1 DNASU 1 DisGeNET 1 EMBL 2 GO 2 Gene3D 2 InterPro 5 NCBI Taxonomy 1 OrthoDB 1 PANTHER 2 PROSITE 5 PROSITE-ProRule 1 PeptideAtlas 1 Pfam 2 RefSeq 1 SMART 2 SUPFAM 2

Citations:

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 611
  • Mass: 71278
  • Checksum: 32538BAE8C8E1532
  • Sequence:
    • MASPSPPPES KEWDYLDPAQ RSLYKDVMME NYGNLVSLDV LNRDKDEEPT VKQEIEEIEE 
EVEPQGVIVT RIKSEIDQDP MGRETFELVG RLDKQRGIFL WEIPRESLTQ EQRMFRENTN 
IIRKRPNSEE KCHKCEECGK GFVRKAHFIQ HQRVHTGEKP FQCNECGKSF SRSSFVIEHQ 
RIHTGERPYE CNYCGKTFSV SSTLIRHQRI HTGERPYQCN QCKQSFSQRR SLVKHQRIHT 
GEKPHKCSDC GKAFSWKSHL IEHQRTHTGE KPYHCTKCKK SFSRNSLLVE HQRIHTGERP 
HKCGECGKAF RLSTYLIQHQ KIHTGEKPFL CIECGKSFSR SSFLIEHQRI HTGERPYQCK 
ECGKSFSQLC NLTRHQRIHT GDKPHKCEEC GKAFSRSSGL IQHQRIHTRE KTYPYNETKE 
SFDPNCSLVI QQEVYPKEKS YKCDECGKTF SVSAHLVQHQ RIHTGEKPYL CTVCGKSFSR 
SSFLIEHQRI HTGERPYLCR QCGKSFSQLC NLIRHQGVHT GNKPHKCDEC GKAFSRNSGL 
IQHQRIHTGE KPYKCEKCDK SFSQQRSLVN HQKIHAEVKT QETHECDACG EAFNCRISLI 
QHQKLHTAWM Q