DCAF5 | ENSG00000139990 | NCBI 8816

Details for: DCAF5

Gene ID: 8816

Gene Type: Protein-coding - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: DCAF5

Ensembl ID: ENSG00000139990

Description: DDB1 and CUL4 associated factor 5

Cell Significance Landscape

Display Count:

Associated with

Metabolism of proteins
(R-HSA-392499)
Neddylation
(R-HSA-8951664)
Post-translational protein modification
(R-HSA-597592)
Cul4-ring e3 ubiquitin ligase complex
(GO:0080008)
Cytoplasm
(GO:0005737)
Negative regulation of fatty acid biosynthetic process
(GO:0045717)
Nucleoplasm
(GO:0005654)
Protein binding
(GO:0005515)
Protein ubiquitination
(GO:0016567)

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040

CSI 20.69

rCSI 50.28%

PRS 27.73
lamp5 GABAergic cortical interneuron CL4023011

CSI 19.52

rCSI 32.77%

PRS 28.54
L6b glutamatergic cortical neuron CL4023038

CSI 18.06

rCSI 56.44%

PRS 29.68
L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041

CSI 15.98

rCSI 57.49%

PRS 27.47
IgG plasma cell CL0000985

CSI 15.63

rCSI 18.72%

PRS 62.7
near-projecting glutamatergic cortical neuron CL4023012

CSI 12.86

rCSI 48.6%

PRS 29.51
corticothalamic-projecting glutamatergic cortical neuron CL4023013

CSI 10

rCSI 58.89%

PRS 29.91
mature B cell CL0000785

CSI 9.93

rCSI 8.63%

PRS 52.48
retinal rod cell CL0000604

CSI 8.15

rCSI 14.37%

PRS 41.93
cardiac endothelial cell CL0010008

CSI 7.97

rCSI 32.16%

PRS 42.05
helper T cell CL0000912

CSI 7.62

rCSI 10.77%

PRS 52.33
cardiac neuron CL0010022

CSI 6.85

rCSI 21.91%

PRS 40.46
kidney loop of Henle thin ascending limb epithelial cell CL1001107

CSI 6.71

rCSI 17.34%

PRS 39.75
neural progenitor cell CL0011020

CSI 6.56

rCSI 28.87%

PRS 37.25
chandelier pvalb GABAergic cortical interneuron CL4023036

CSI 6.44

rCSI 20.13%

PRS 31.91
class switched memory B cell CL0000972

CSI 6.26

rCSI 4.67%

PRS 61.78
neuroendocrine cell CL0000165

CSI 6.13

rCSI 23.69%

PRS 62
nasal mucosa goblet cell CL0002480

CSI 6.04

rCSI 7.01%

PRS 54.2
sncg GABAergic cortical interneuron CL4023015

CSI 5.8

rCSI 9.33%

PRS 30.6
central nervous system neuron CL2000029

CSI 5.72

rCSI 42.07%

PRS 32.05
enteroendocrine cell CL0000164

CSI 5.71

rCSI 7.8%

PRS 45.91
mesodermal cell CL0000222

CSI 5.42

rCSI 6.5%

PRS 41.54
Kupffer cell CL0000091

CSI 5.16

rCSI 11.8%

PRS 42.83
retinal ganglion cell CL0000740

CSI 5.01

rCSI 11.06%

PRS 32.38
rod bipolar cell CL0000751

CSI 4.95

rCSI 8.89%

PRS 37.12
cerebral cortex endothelial cell CL1001602

CSI 4.79

rCSI 8.29%

PRS 34.6
retina horizontal cell CL0000745

CSI 4.54

rCSI 6.92%

PRS 40.25
caudal ganglionic eminence derived cortical interneuron CL4023064

CSI 4.47

rCSI 7.89%

PRS 27.56
alpha-beta T cell CL0000789

CSI 4.42

rCSI 5.18%

PRS 58.87
dopaminergic neuron CL0000700

CSI 4.37

rCSI 24.69%

PRS 30.5
glioblast CL0000030

CSI 4.35

rCSI 6.94%

PRS 37.55
alveolar macrophage CL0000583

CSI 4.32

rCSI 7.12%

PRS 48.87
effector CD8-positive, alpha-beta T cell CL0001050

CSI 4.14

rCSI 3.15%

PRS 54.57
unswitched memory B cell CL0000970

CSI 4

rCSI 3.36%

PRS 60.59
pvalb GABAergic cortical interneuron CL4023018

CSI 3.88

rCSI 4.82%

PRS 27.1
colonocyte CL1000347

CSI 3.86

rCSI 5.54%

PRS 50.68
Mueller cell CL0000636

CSI 3.85

rCSI 8.79%

PRS 37.26
neuroblast (sensu Vertebrata) CL0000031

CSI 3.85

rCSI 4.94%

PRS 41.49
renal alpha-intercalated cell CL0005011

CSI 3.8

rCSI 5.08%

PRS 51.6
double negative thymocyte CL0002489

CSI 3.78

rCSI 2.63%

PRS 51.7
double-positive, alpha-beta thymocyte CL0000809

CSI 3.77

rCSI 3.84%

PRS 56.32
respiratory hillock cell CL4030023

CSI 3.74

rCSI 6.66%

PRS 58.96
pulmonary artery endothelial cell CL1001568

CSI 3.71

rCSI 5.05%

PRS 56.28
myoepithelial cell CL0000185

CSI 3.45

rCSI 8.72%

PRS 51.57
pancreatic D cell CL0000173

CSI 3.39

rCSI 3.33%

PRS 45.57
melanocyte CL0000148

CSI 3.37

rCSI 2.49%

PRS 37.38
epithelial cell of lower respiratory tract CL0002632

CSI 3.33

rCSI 2.58%

PRS 43.81
small intestine goblet cell CL1000495

CSI 3.25

rCSI 7.11%

PRS 53.34
CD4-positive, alpha-beta memory T cell CL0000897

CSI 3.24

rCSI 2.33%

PRS 56.11
transit amplifying cell of colon CL0009011

CSI 3.1

rCSI 3.64%

PRS 47.09
mature T cell CL0002419

CSI 3.07

rCSI 2.39%

PRS 60.11
intestine goblet cell CL0019031

CSI 3.01

rCSI 2.67%

PRS 42.48
kidney interstitial alternatively activated macrophage CL1000695

CSI 3

rCSI 7.81%

PRS 42.58
type B pancreatic cell CL0000169

CSI 2.98

rCSI 6.59%

PRS 40.75
interstitial cell of Cajal CL0002088

CSI 2.97

rCSI 3.78%

PRS 48.5
pulmonary capillary endothelial cell CL4028001

CSI 2.94

rCSI 5.62%

PRS 60.13
lung pericyte CL0009089

CSI 2.81

rCSI 7.42%

PRS 50.78
VIP GABAergic cortical interneuron CL4023016

CSI 2.79

rCSI 3.33%

PRS 28.52
direct pathway medium spiny neuron CL4023026

CSI 2.75

rCSI 65.91%

PRS 28.05
radial glial cell CL0000681

CSI 2.75

rCSI 3.82%

PRS 42.88
neural crest cell CL0011012

CSI 2.74

rCSI 2.17%

PRS 31.76
group 3 innate lymphoid cell CL0001071

CSI 2.72

rCSI 2.04%

PRS 46.65
vascular leptomeningeal cell CL4023051

CSI 2.65

rCSI 4.65%

PRS 36.12
precursor B cell CL0000817

CSI 2.63

rCSI 2.31%

PRS 53.04
adipocyte CL0000136

CSI 2.63

rCSI 3.38%

PRS 39.26
interneuron CL0000099

CSI 2.61

rCSI 5.24%

PRS 33.82
indirect pathway medium spiny neuron CL4023029

CSI 2.59

rCSI 62.52%

PRS 28.93
goblet cell CL0000160

CSI 2.59

rCSI 2.45%

PRS 44.37
central memory CD8-positive, alpha-beta T cell CL0000907

CSI 2.58

rCSI 1.74%

PRS 53.42
brush cell of tracheobronchial tree CL0002075

CSI 2.55

rCSI 7.56%

PRS 55.11
intestinal epithelial cell CL0002563

CSI 2.55

rCSI 2.66%

PRS 42.99
mononuclear phagocyte CL0000113

CSI 2.54

rCSI 5.6%

PRS 47.36
cerebellar neuron CL1001611

CSI 2.53

rCSI 22.27%

PRS 32.68
renal principal cell CL0005009

CSI 2.52

rCSI 6.55%

PRS 48.65
pulmonary alveolar type 1 cell CL0002062

CSI 2.51

rCSI 14.48%

PRS 45.66
secretory cell CL0000151

CSI 2.51

rCSI 2.61%

PRS 44.07
fibroblast of lung CL0002553

CSI 2.51

rCSI 2.33%

PRS 43.27
cerebellar granule cell CL0001031

CSI 2.5

rCSI 3.67%

PRS 39.72
activated CD4-positive, alpha-beta T cell CL0000896

CSI 2.47

rCSI 2.28%

PRS 63.56
hepatic stellate cell CL0000632

CSI 2.47

rCSI 9.25%

PRS 36.88
GABAergic interneuron CL0011005

CSI 2.42

rCSI 38.18%

PRS 47.87
effector CD4-positive, alpha-beta T cell CL0001044

CSI 2.42

rCSI 6.94%

PRS 60.65
CD14-positive monocyte CL0001054

CSI 2.41

rCSI 3.01%

PRS 54.77
naive T cell CL0000898

CSI 2.39

rCSI 1.66%

PRS 56.09
ciliated epithelial cell CL0000067

CSI 2.36

rCSI 2.08%

PRS 33.09
plasmablast CL0000980

CSI 2.36

rCSI 1.85%

PRS 49.73
inhibitory interneuron CL0000498

CSI 2.35

rCSI 5.42%

PRS 35.73
perivascular cell CL4033054

CSI 2.34

rCSI 3.19%

PRS 48.1
central memory CD4-positive, alpha-beta T cell CL0000904

CSI 2.33

rCSI 1.38%

PRS 58.18
blood vessel endothelial cell CL0000071

CSI 2.33

rCSI 4.83%

PRS 41.42
fibroblast of cardiac tissue CL0002548

CSI 2.3

rCSI 11.04%

PRS 41.98
alveolar type 1 fibroblast cell CL4028004

CSI 2.29

rCSI 2.51%

PRS 47.18
microcirculation associated smooth muscle cell CL0008035

CSI 2.27

rCSI 6.57%

PRS 45.9
endocardial cell CL0002350

CSI 2.25

rCSI 10.77%

PRS 45.21
macroglial cell CL0000126

CSI 2.23

rCSI 5.73%

PRS 46.42
cerebral cortex GABAergic interneuron CL0010011

CSI 2.23

rCSI 6.58%

PRS 47.36
elicited macrophage CL0000861

CSI 2.22

rCSI 2.04%

PRS 50.58
pulmonary alveolar type 2 cell CL0002063

CSI 2.21

rCSI 3.42%

PRS 53.41
lung neuroendocrine cell CL1000223

CSI 2.16

rCSI 3.2%

PRS 48.24
ciliated cell CL0000064

CSI 2.16

rCSI 3.49%

PRS 42.01

respiratory goblet cell CL0002370

CSI 0.2

rCSI 1.9%

PRS 64.0%
regular ventricular cardiac myocyte CL0002131

CSI 0.3

rCSI 2.0%

PRS 36.3%
mesangial cell CL0000650

CSI 0.3

rCSI 1.3%

PRS 55.2%
podocyte CL0000653

CSI 0.4

rCSI 1.7%

PRS 42.1%
decidual natural killer cell, human CL0002343

CSI 0.4

rCSI 4.0%

PRS 83.2%
amacrine cell CL0000561

CSI 0.4

rCSI 1.3%

PRS 35.4%
pancreatic ductal cell CL0002079

CSI 0.5

rCSI 1.0%

PRS 45.3%
effector memory CD8-positive, alpha-beta T cell, terminally differentiated CL0001062

CSI 0.6

rCSI 2.8%

PRS 54.2%
extravillous trophoblast CL0008036

CSI 0.6

rCSI 0.7%

PRS 39.4%
pancreatic acinar cell CL0002064

CSI 0.6

rCSI 0.8%

PRS 48.0%
midbrain dopaminergic neuron CL2000097

CSI 0.7

rCSI 4.3%

PRS 49.9%
cholangiocyte CL1000488

CSI 0.7

rCSI 4.1%

PRS 57.7%
intermediate monocyte CL0002393

CSI 0.7

rCSI 1.0%

PRS 45.3%
stromal cell CL0000499

CSI 0.7

rCSI 2.0%

PRS 43.9%
Hofbauer cell CL3000001

CSI 0.7

rCSI 1.4%

PRS 53.5%
glutamatergic neuron CL0000679

CSI 0.7

rCSI 1.5%

PRS 38.4%
kidney distal convoluted tubule epithelial cell CL1000849

CSI 0.8

rCSI 8.2%

PRS 45.0%
GABAergic amacrine cell CL4030027

CSI 0.8

rCSI 2.7%

PRS 35.6%
centrilobular region hepatocyte CL0019029

CSI 0.8

rCSI 2.1%

PRS 51.7%
endothelial cell of placenta CL0009092

CSI 0.8

rCSI 4.0%

PRS 55.0%
regular atrial cardiac myocyte CL0002129

CSI 0.8

rCSI 2.6%

PRS 42.8%
ciliated columnar cell of tracheobronchial tree CL0002145

CSI 0.8

rCSI 1.9%

PRS 42.1%
paneth cell CL0000510

CSI 0.8

rCSI 1.3%

PRS 60.7%
duct epithelial cell CL0000068

CSI 0.9

rCSI 1.3%

PRS 46.3%
L4 intratelencephalic projecting glutamatergic neuron CL4030063

CSI 0.9

rCSI 2.1%

PRS 33.5%
pancreatic A cell CL0000171

CSI 0.9

rCSI 0.9%

PRS 46.0%
H1 horizontal cell CL0004217

CSI 0.9

rCSI 3.6%

PRS 46.4%
serotonergic neuron CL0000850

CSI 0.9

rCSI 4.2%

PRS 30.8%
paneth cell of epithelium of small intestine CL1000343

CSI 1.0

rCSI 2.7%

PRS 57.8%
acinar cell CL0000622

CSI 1.0

rCSI 1.4%

PRS 54.3%
alternatively activated macrophage CL0000890

CSI 1.0

rCSI 1.2%

PRS 56.7%
parietal epithelial cell CL1000452

CSI 1.0

rCSI 2.7%

PRS 36.7%
glial cell CL0000125

CSI 1.0

rCSI 3.9%

PRS 37.3%
ON parasol ganglion cell CL4033052

CSI 1.1

rCSI 15.0%

PRS 36.4%
megakaryocyte-erythroid progenitor cell CL0000050

CSI 1.1

rCSI 1.0%

PRS 40.2%
intestinal tuft cell CL0019032

CSI 1.1

rCSI 1.7%

PRS 48.0%
tuft cell of colon CL0009041

CSI 1.1

rCSI 2.6%

PRS 61.5%
innate lymphoid cell CL0001065

CSI 1.1

rCSI 2.3%

PRS 50.1%
retinal cone cell CL0000573

CSI 1.1

rCSI 1.8%

PRS 34.6%
keratinocyte CL0000312

CSI 1.2

rCSI 1.0%

PRS 48.3%
medium spiny neuron CL1001474

CSI 1.2

rCSI 10.3%

PRS 31.6%
club cell CL0000158

CSI 1.2

rCSI 1.8%

PRS 43.1%
kidney connecting tubule epithelial cell CL1000768

CSI 1.2

rCSI 3.1%

PRS 34.5%
epicardial adipocyte CL1000309

CSI 1.2

rCSI 4.0%

PRS 45.7%
dendritic cell, human CL0001056

CSI 1.3

rCSI 1.9%

PRS 50.2%
cardiac muscle cell CL0000746

CSI 1.3

rCSI 1.8%

PRS 35.2%
retinal blood vessel endothelial cell CL0002585

CSI 1.3

rCSI 2.0%

PRS 47.1%
myeloid dendritic cell CL0000782

CSI 1.3

rCSI 1.9%

PRS 59.5%
L2/3 intratelencephalic projecting glutamatergic neuron CL4030059

CSI 1.3

rCSI 2.8%

PRS 34.2%
GABAergic neuron CL0000617

CSI 1.3

rCSI 4.4%

PRS 32.5%
ionocyte CL0005006

CSI 1.4

rCSI 1.5%

PRS 41.4%
glycinergic amacrine cell CL4030028

CSI 1.4

rCSI 3.6%

PRS 43.4%
keratocyte CL0002363

CSI 1.4

rCSI 3.3%

PRS 53.5%
Bergmann glial cell CL0000644

CSI 1.4

rCSI 1.9%

PRS 40.1%
periportal region hepatocyte CL0019026

CSI 1.4

rCSI 5.4%

PRS 52.2%
lung resident memory CD8-positive, alpha-beta T cell CL4033039

CSI 1.4

rCSI 3.7%

PRS 74.6%
retinal bipolar neuron CL0000748

CSI 1.5

rCSI 2.8%

PRS 33.7%
respiratory basal cell CL0002633

CSI 1.5

rCSI 1.5%

PRS 49.1%
activated CD8-positive, alpha-beta T cell, human CL0001049

CSI 1.5

rCSI 2.5%

PRS 64.2%
erythroblast CL0000765

CSI 1.5

rCSI 3.9%

PRS 56.8%
conjunctival epithelial cell CL1000432

CSI 1.5

rCSI 2.3%

PRS 44.0%
endothelial cell of pericentral hepatic sinusoid CL0019022

CSI 1.5

rCSI 4.7%

PRS 55.6%
epithelial cell of proximal tubule CL0002306

CSI 1.5

rCSI 3.8%

PRS 39.7%
stem cell CL0000034

CSI 1.6

rCSI 1.5%

PRS 34.5%
L5/6 near-projecting glutamatergic neuron CL4030067

CSI 1.6

rCSI 5.1%

PRS 32.9%
renal interstitial pericyte CL1001318

CSI 1.6

rCSI 4.3%

PRS 40.4%
choroid plexus epithelial cell CL0000706

CSI 1.6

rCSI 2.6%

PRS 34.6%
lung macrophage CL1001603

CSI 1.6

rCSI 3.6%

PRS 49.8%
CD1c-positive myeloid dendritic cell CL0002399

CSI 1.6

rCSI 2.0%

PRS 51.0%
BEST4+ enteroycte CL4030026

CSI 1.6

rCSI 2.0%

PRS 46.0%
renal beta-intercalated cell CL0002201

CSI 1.6

rCSI 3.9%

PRS 45.7%
CD8-positive, CD28-negative, alpha-beta regulatory T cell CL0000920

CSI 1.6

rCSI 3.3%

PRS 61.2%
kidney loop of Henle thin descending limb epithelial cell CL1001111

CSI 1.6

rCSI 2.3%

PRS 40.5%
fallopian tube secretory epithelial cell CL4030006

CSI 1.7

rCSI 1.6%

PRS 43.9%
tracheobronchial smooth muscle cell CL0019019

CSI 1.7

rCSI 2.9%

PRS 51.9%
pulmonary ionocyte CL0017000

CSI 1.7

rCSI 2.0%

PRS 50.9%
lung secretory cell CL1000272

CSI 1.7

rCSI 4.1%

PRS 41.5%
brush cell CL0002204

CSI 1.7

rCSI 3.3%

PRS 69.2%
OFF midget ganglion cell CL4033047

CSI 1.7

rCSI 34.4%

PRS 37.7%
sst GABAergic cortical interneuron CL4023017

CSI 1.7

rCSI 2.2%

PRS 29.4%
chondrocyte CL0000138

CSI 1.7

rCSI 2.7%

PRS 36.9%
mature astrocyte CL0002627

CSI 1.7

rCSI 7.2%

PRS 41.0%
lymphoid lineage restricted progenitor cell CL0000838

CSI 1.7

rCSI 6.6%

PRS 63.7%
colon epithelial cell CL0011108

CSI 1.7

rCSI 1.8%

PRS 40.8%
astrocyte of the cerebral cortex CL0002605

CSI 1.7

rCSI 3.9%

PRS 29.2%
enteric smooth muscle cell CL0002504

CSI 1.8

rCSI 2.5%

PRS 46.0%
respiratory suprabasal cell CL4033048

CSI 1.8

rCSI 2.3%

PRS 48.1%
corneal epithelial cell CL0000575

CSI 1.8

rCSI 5.1%

PRS 60.5%
hepatocyte CL0000182

CSI 1.8

rCSI 3.2%

PRS 41.8%
alveolar adventitial fibroblast CL4028006

CSI 1.8

rCSI 2.9%

PRS 44.3%
enteroendocrine cell of small intestine CL0009006

CSI 1.8

rCSI 4.0%

PRS 57.9%
bronchus fibroblast of lung CL2000093

CSI 1.8

rCSI 1.5%

PRS 44.2%
Schwann cell CL0002573

CSI 1.8

rCSI 5.2%

PRS 43.5%
lung ciliated cell CL1000271

CSI 1.8

rCSI 2.1%

PRS 34.0%
squamous epithelial cell CL0000076

CSI 1.9

rCSI 4.4%

PRS 49.2%
CD8-positive, alpha-beta cytotoxic T cell CL0000794

CSI 1.9

rCSI 2.2%

PRS 63.6%
neuroblast (sensu Nematoda and Protostomia) CL0000338

CSI 1.9

rCSI 2.2%

PRS 38.0%
mucus secreting cell CL0000319

CSI 1.9

rCSI 3.0%

PRS 53.8%
myeloid leukocyte CL0000766

CSI 1.9

rCSI 1.8%

PRS 44.3%
ependymal cell CL0000065

CSI 1.9

rCSI 3.9%

PRS 26.8%

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Interaction Source:

Pathway Categories (for ONTOLOGY source):

Baseline Cell Type:

Baseline Cell Context(s): Comma-separated if multiple.

Target Cell Type:

Target Cell Context(s): Comma-separated if multiple.

Legend:

Query Gene
Node Color (Target Cell CSI, relative to current network):
- Very High
- High
- Medium
- Low
- Very Low
- CSI N/A
Node Size: Proportional to Target Cell CSI magnitude
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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

Description
Proteins

## Summary [DCAF5](/details-gene/8816) (DDB1 and CUL4 associated factor 5) is a protein-coding gene located on chromosome 14q24.1. It functions as a substrate receptor component of the CUL4-DDB1 E3 ubiquitin ligase complex, which targets proteins for proteasomal degradation ([Link](https://doi.org/10.1016/j.molcel.2006.08.010), [Link](https://doi.org/10.1038/nature05175)). The complex plays a critical role in protein ubiquitination, and [DCAF5](/details-gene/8816) specifically mediates the degradation of methylated proteins, including key epigenetic regulators and transcription factors like DNMT1 and SOX2 ([Link](https://doi.org/10.1038/s41467-018-04019-9), [Link](https://doi.org/10.1074/jbc.ra118.005336)). **Overall**, expression data indicate that [DCAF5](/details-gene/8816) is a highly significant gene in the central nervous system, with prominent expression across diverse neuronal subtypes, including glutamatergic and GABAergic neurons. It also shows notable significance in specific immune cell populations, particularly those of the B cell lineage. ## Cellular Roles and Expression Landscape The expression profile of [DCAF5](/details-gene/8816) suggests a primary role in the function and maintenance of the mammalian nervous system. It exhibits its highest significance scores in a wide array of cortical neurons, including [L2/3-6 intratelencephalic projecting glutamatergic neuron](/details-cell/CL4023040) (CSI: 20.69), [lamp5 GABAergic cortical interneuron](/details-cell/CL4023011) (CSI: 19.52), and [L6b glutamatergic cortical neuron](/details-cell/CL4023038) (CSI: 18.06). This broad but high expression across different neuronal classes suggests a fundamental role in neuronal protein homeostasis rather than a function specific to a single neuronal identity. Its significance in [neural progenitor cell](/details-cell/CL0011020) is consistent with its known function in degrading the pluripotency factor SOX2, a key step in cellular differentiation. Beyond the central nervous system, [DCAF5](/details-gene/8816) demonstrates a distinct and significant role within the adaptive immune system. It is highly expressed in terminally differentiated [IgG plasma cell](/details-cell/CL0000985) (CSI: 15.63) and their precursors, [mature B cell](/details-cell/CL0000785) (CSI: 9.93), as well as in [helper T cell](/details-cell/CL0000912) (CSI: 7.62). This pattern suggests that [DCAF5](/details-gene/8816)-mediated protein degradation may be involved in the processes of lymphocyte maturation, activation, and effector function. The gene's expression in other tissues, such as [retinal rod cell](/details-cell/CL0000604) and [cardiac endothelial cell](/details-cell/CL0010008), indicates a broader functional relevance, although its most prominent roles appear to be concentrated in neuronal and immune contexts. ## Pathways and Molecular Function Functionally, [DCAF5](/details-gene/8816) is annotated as a core component of the [Cul4-ring e3 ubiquitin ligase complex](/details-ontology/GO:0080008), localizing to both the [cytoplasm](/details-ontology/GO:0005737) and [nucleoplasm](/details-ontology/GO:0005654). Its primary molecular function is to confer substrate specificity to this complex, facilitating [protein ubiquitination](/details-ontology/GO:0016567) and subsequent proteolysis. This role places it within the broader Reactome pathways of [Metabolism of proteins](/details-pathway/R-HSA-392499) and [Post-translational protein modification](/details-pathway/R-HSA-597592). Recent research has identified specific substrates for the CRL4-[DCAF5](/details-gene/8816) complex, revealing its importance in epigenetic regulation and developmental processes. The complex recognizes and targets methylated lysine residues on proteins, including the DNA methyltransferase DNMT1 and the transcription factor E2F1 ([Link](https://doi.org/10.1038/s41467-018-04019-9)), as well as the pluripotency-associated transcription factor SOX2 ([Link](https://doi.org/10.1074/jbc.ra118.005336)). By mediating the degradation of these key regulatory proteins, [DCAF5](/details-gene/8816) likely plays a crucial role in cell cycle control, cellular differentiation, and the maintenance of epigenetic landscapes. Its annotation for involvement in the [negative regulation of fatty acid biosynthetic process](/details-ontology/GO:0045717) suggests additional metabolic functions that warrant further investigation. ## Research Directions The specific expression patterns and known molecular functions of [DCAF5](/details-gene/8816) lead to several testable hypotheses regarding its physiological roles. 1. **Hypothesis 1:** Given its high expression across multiple mature neuronal subtypes and its role in degrading the neural stem cell factor SOX2, [DCAF5](/details-gene/8816) may be a critical factor for maintaining the post-mitotic state and terminal identity of neurons by continuously clearing residual developmental proteins. 2. **Hypothesis 2:** The high significance of [DCAF5](/details-gene/8816) in [IgG plasma cell](/details-cell/CL0000985) and its ability to target the epigenetic writer DNMT1 for degradation suggest that [DCAF5](/details-gene/8816) facilitates the extensive epigenetic reprogramming required for a B cell to differentiate into a high-rate, antibody-secreting plasma cell. To test the second hypothesis, a key experiment could be proposed. One could generate a conditional knockout mouse model where [DCAF5](/details-gene/8816) is specifically deleted in the B cell lineage (e.g., using a Cd19-Cre driver). B cells from these mice and wild-type controls could be isolated and stimulated *ex vivo* with LPS and IL-4 to induce plasma cell differentiation. The efficiency of this process could be quantified by flow cytometry for plasma cell markers (CD138), while immunoglobulin secretion could be measured by ELISA. Parallel western blot or proteomic analysis could be used to confirm the expected stabilization of DNMT1 in the knockout cells, linking the molecular mechanism to the cellular phenotype. From a therapeutic perspective, [DCAF5](/details-gene/8816) presents an intriguing target. As a substrate receptor for an E3 ligase, it is well-suited for the development of molecular glue degraders or Proteolysis Targeting Chimeras (PROTACs). Instead of inhibiting [DCAF5](/details-gene/8816), a more promising strategy would be to design molecules that enhance or redirect its activity. For example, in cancers driven by the overexpression of oncoproteins like SOX2 or DNMT1, a therapeutic could be developed to specifically enhance the binding of these targets to [DCAF5](/details-gene/8816), promoting their degradation. This activation/redirection strategy could offer a highly specific means of eliminating cancer-driving proteins, though delivery to target tissues, particularly the brain, would be a significant challenge.

Disclaimer: This in-silico analysis is generated by an AI language model and may contain inaccuracies or hallucinations. However, it is cross-referenced with curated gene expression data from major biological sources. Please verify the information before use.

DDB1- and CUL4-associated factor 5
Q8TBB7_HUMAN

Genular Protein ID: 2059340477

Symbol: DCAF5_HUMAN

Name: DDB1- and CUL4-associated factor 5

UniProtKB Accession Codes:

Q96JK2 B2RN31 G3V4J7 O60559 Q8N3V3 Q8N3V5

Database IDs:

Citations:

PubMed ID: 11347906

Title: Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.

PubMed ID: 11347906

DOI: 10.1093/dnares/8.2.85

PubMed ID: 17974005

Title: The full-ORF clone resource of the German cDNA consortium.

PubMed ID: 17974005

DOI: 10.1186/1471-2164-8-399

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 9740667

Title: Genomic and functional map of the chromosome 14 t(12;14) breakpoint cluster region in uterine leiomyoma.

PubMed ID: 9740667

DOI: 10.1006/geno.1998.5406

PubMed ID: 16949367

Title: A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1.

PubMed ID: 16949367

DOI: 10.1016/j.molcel.2006.08.010

PubMed ID: 16964240

Title: Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery.

PubMed ID: 16964240

DOI: 10.1038/nature05175

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 29691401

Title: Methylated DNMT1 and E2F1 are targeted for proteolysis by L3MBTL3 and CRL4-DCAF5 ubiquitin ligase.

PubMed ID: 29691401

DOI: 10.1038/s41467-018-04019-9

PubMed ID: 30442713

Title: Proteolysis of methylated SOX2 protein is regulated by L3MBTL3 and CRL4-DCAF5 ubiquitin ligase.

PubMed ID: 30442713

DOI: 10.1074/jbc.ra118.005336

Sequence Information:

Length: 942
Mass: 103963
Checksum: 3C627BA3FB2DC522
Sequence:

MKRRAGLGGS MRSVVGFLSQ RGLHGDPLLT QDFQRRRLRG CRNLYKKDLL GHFGCVNAIE 
FSNNGGQWLV SGGDDRRVLL WHMEQAIHSR VKPIQLKGEH HSNIFCLAFN SGNTKVFSGG 
NDEQVILHDV ESSETLDVFA HEDAVYGLSV SPVNDNIFAS SSDDGRVLIW DIRESPHGEP 
FCLANYPSAF HSVMFNPVEP RLLATANSKE GVGLWDIRKP QSSLLRYGGN LSLQSAMSVR 
FNSNGTQLLA LRRRLPPVLY DIHSRLPVFQ FDNQGYFNSC TMKSCCFAGD RDQYILSGSD 
DFNLYMWRIP ADPEAGGIGR VVNGAFMVLK GHRSIVNQVR FNPHTYMICS SGVEKIIKIW 
SPYKQPGCTG DLDGRIEDDS RCLYTHEEYI SLVLNSGSGL SHDYANQSVQ EDPRMMAFFD 
SLVRREIEGW SSDSDSDLSE STILQLHAGV SERSGYTDSE SSASLPRSPP PTVDESADNA 
FHLGPLRVTT TNTVASTPPT PTCEDAASRQ QRLSALRRYQ DKRLLALSNE SDSEENVCEV 
ELDTDLFPRP RSPSPEDESS SSSSSSSSED EEELNERRAS TWQRNAMRRR QKTTREDKPS 
APIKPTNTYI GEDNYDYPQI KVDDLSSSPT SSPERSTSTL EIQPSRASPT SDIESVERKI 
YKAYKWLRYS YISYSNNKDG ETSLVTGEAD EGRAGTSHKD NPAPSSSKEA CLNIAMAQRN 
QDLPPEGCSK DTFKEETPRT PSNGPGHEHS SHAWAEVPEG TSQDTGNSGS VEHPFETKKL 
NGKALSSRAE EPPSPPVPKA SGSTLNSGSG NCPRTQSDDS EERSLETICA NHNNGRLHPR 
PPHPHNNGQN LGELEVVAYS SPGHSDTDRD NSSLTGTLLH KDCCGSEMAC ETPNAGTRED 
PTDTPATDSS RAVHGHSGLK RQRIELEDTD SENSSSEKKL KT

Genular Protein ID: 1899331210

Symbol: Q8TBB7_HUMAN

Name: N/A

UniProtKB Accession Codes:

Q8TBB7

Database IDs:

Citations:

PubMed ID: 11237011

Title: Initial sequencing and analysis of the human genome.

PubMed ID: 11237011

DOI: 10.1038/35057062

PubMed ID: 11181995

Title: The sequence of the human genome.

PubMed ID: 11181995

DOI: 10.1126/science.1058040

PubMed ID: 12508121

Title: The DNA sequence and analysis of human chromosome 14.

PubMed ID: 12508121

DOI: 10.1038/nature01348

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 15496913

Title: Finishing the euchromatic sequence of the human genome.

PubMed ID: 15496913

DOI: 10.1038/nature03001

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

Sequence Information:

Length: 326
Mass: 36590
Checksum: B56EF8095C053217
Sequence:

MKRRAGLGGS MRSVVGFLSQ RGLHGDPLLT QDFQRRRLRG CRNLYKKDLL GHFGCVNAIE 
FSNNGGQWLV SGGDDRRVLL WHMEQAIHSR VKPIQLKGEH HSNIFCLAFN SGNTKVFSGG 
NDEQVILHDV ESSETLDVFA HEDAVYGLSV SPVNDNIFAS SSDDGRVLIW DIRESPHGEP 
FCLANYPSAF HSVMFNPVEP RLLATANSKE GVGLWDIRKP QSSLLRYGGN LSLQSAMSVR 
FNSNGTQLLA LRRRLPPVLY DIHSRLPVFQ FDNQGYFNSC TMKSCCFAGD RDQHHNWKWR 
AQAVSFQPFL SLTRNGDADV DLKLAV

Details for: DCAF5

Cell Significance Landscape

Associated with

Significant Cells

Network Configuration

Legend:

Other Information

Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. PubMed ID: 11347906

The full-ORF clone resource of the German cDNA consortium. PubMed ID: 17974005

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). PubMed ID: 15489334

Genomic and functional map of the chromosome 14 t(12;14) breakpoint cluster region in uterine leiomyoma. PubMed ID: 9740667

A family of diverse Cul4-Ddb1-interacting proteins includes Cdt2, which is required for S phase destruction of the replication factor Cdt1. PubMed ID: 16949367

Molecular architecture and assembly of the DDB1-CUL4A ubiquitin ligase machinery. PubMed ID: 16964240

Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. PubMed ID: 19690332

Initial characterization of the human central proteome. PubMed ID: 21269460

Toward a comprehensive characterization of a human cancer cell phosphoproteome. PubMed ID: 23186163

An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. PubMed ID: 24275569

Methylated DNMT1 and E2F1 are targeted for proteolysis by L3MBTL3 and CRL4-DCAF5 ubiquitin ligase. PubMed ID: 29691401

Proteolysis of methylated SOX2 protein is regulated by L3MBTL3 and CRL4-DCAF5 ubiquitin ligase. PubMed ID: 30442713

Initial sequencing and analysis of the human genome. PubMed ID: 11237011

The sequence of the human genome. PubMed ID: 11181995

The DNA sequence and analysis of human chromosome 14. PubMed ID: 12508121

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). PubMed ID: 15489334

Finishing the euchromatic sequence of the human genome. PubMed ID: 15496913

Initial characterization of the human central proteome. PubMed ID: 21269460

PubMed ID: 11347906

PubMed ID: 17974005

PubMed ID: 15489334

PubMed ID: 9740667

PubMed ID: 16949367

PubMed ID: 16964240

PubMed ID: 19690332

PubMed ID: 21269460

PubMed ID: 23186163

PubMed ID: 24275569

PubMed ID: 29691401

PubMed ID: 30442713

PubMed ID: 11237011

PubMed ID: 11181995

PubMed ID: 12508121

PubMed ID: 15489334

PubMed ID: 15496913

PubMed ID: 21269460