Details for: CPNE7

Gene ID: 27132

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: CPNE7

Ensembl ID: ENSG00000178773

Description: copine 7

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • group 3 innate lymphoid cell CL0001071
    CSI 3.49
    rCSI 2.63%
    PRS 97.16
  • sst GABAergic cortical interneuron CL4023017
    CSI 2.95
    rCSI 3.8%
    PRS 89.04
  • VIP GABAergic cortical interneuron CL4023016
    CSI 2.44
    rCSI 2.92%
    PRS 88.17
  • effector CD4-positive, alpha-beta T cell CL0001044
    CSI 2.19
    rCSI 6.29%
    PRS 99.38
  • CD8-positive, CD28-negative, alpha-beta regulatory T cell CL0000920
    CSI 1.75
    rCSI 3.48%
    PRS 98.39
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 1.69
    rCSI 2.99%
    PRS 87.72
  • amacrine cell CL0000561
    CSI 1.67
    rCSI 4.85%
    PRS 90.21
  • sncg GABAergic cortical interneuron CL4023015
    CSI 1.6
    rCSI 2.57%
    PRS 88.95
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 1.51
    rCSI 3.67%
    PRS 86.4
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 1.26
    rCSI 2.12%
    PRS 88.21
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 1.21
    rCSI 4.59%
    PRS 88.22
  • L6b glutamatergic cortical neuron CL4023038
    CSI 0.79
    rCSI 2.45%
    PRS 88.95

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [CPNE7](/details-gene/27132) (Copine 7) is a protein-coding gene located on chromosome 16q24.3. It belongs to the copine family of calcium-dependent, phospholipid-binding proteins. Functionally, [CPNE7](/details-gene/27132) is involved in processes that respond to intracellular calcium transients, such as [lipid metabolic process](/details-go/GO:0006629) and membrane trafficking [Link](https://doi.org/10.1111/j.1742-4658.2010.07935.x). **Overall**, its expression profile indicates a significant and distinct role in two major biological systems: the immune system, with particularly high significance in [group 3 innate lymphoid cell](/details-cell/CL0001071), and the central nervous system, where it is a prominent marker for various subtypes of cortical interneurons, including [sst GABAergic cortical interneuron](/details-cell/CL4023017) and [VIP GABAergic cortical interneuron](/details-cell/CL4023016). ## Cellular Roles and Expression Landscape The expression landscape of [CPNE7](/details-gene/27132) reveals a specialized role within specific cell populations of both the nervous and immune systems. **Overall**, its most significant expression is observed in [group 3 innate lymphoid cell](/details-cell/CL0001071) (CSI: 3.49), suggesting a function in innate mucosal immunity. It also shows high significance in adaptive immune cells, including [effector CD4-positive, alpha-beta T cell](/details-cell/CL0001044) (CSI: 2.19) and [CD8-positive, CD28-negative, alpha-beta regulatory T cell](/details-cell/CL0000920) (CSI: 1.75), which may point to a role in T cell activation or regulation following calcium influx. Concurrently, [CPNE7](/details-gene/27132) is highly significant across a diverse array of neuronal subtypes, particularly in the cerebral cortex. It is a key marker for several GABAergic cortical interneuron populations, including [sst GABAergic cortical interneuron](/details-cell/CL4023017) (CSI: 2.95), [VIP GABAergic cortical interneuron](/details-cell/CL4023016) (CSI: 2.44), and [caudal ganglionic eminence derived cortical interneuron](/details-cell/CL4023064) (CSI: 1.69). Its expression also extends to glutamatergic neurons, such as [L2/3-6 intratelencephalic projecting glutamatergic neuron](/details-cell/CL4023040) (CSI: 1.51). This broad yet specific expression pattern in the CNS, including in retinal [amacrine cell](/details-cell/CL0000561) (CSI: 1.67), suggests a fundamental role in neuronal function, likely related to calcium-mediated signaling at the [synapse](/details-go/GO:0045202). ## Pathways and Molecular Function The molecular function of [CPNE7](/details-gene/27132) is centered on its ability to perform [calcium-dependent phospholipid binding](/details-go/GO:0005544), a role shared by other members of the copine family [Link](https://doi.org/10.1111/j.1742-4658.2010.07935.x). This core function links it directly to the biological process of [cellular response to calcium ion](/details-go/GO:0071277). In the presence of elevated intracellular calcium, [CPNE7](/details-gene/27132) is thought to translocate to cellular membranes, including the [plasma membrane](/details-go/GO:0005886), where it can interact with lipids and other proteins. This mechanism is consistent with its involvement in [Metabolism of lipids](/details-pathway/R-HSA-556833) and specifically [Phospholipid metabolism](/details-pathway/R-HSA-1483257), as annotated by Reactome. Within cells, [CPNE7](/details-gene/27132) has been localized to the [cytoplasm](/details-go/GO:0005737), [nucleus](/details-go/GO:0005634), and [synapse](/details-go/GO:0045202), and has also been detected in [extracellular exosome](/details-go/GO:0070062). Its presence at the synapse is particularly relevant given its high expression in neurons, suggesting a potential role in neurotransmitter release or postsynaptic signaling, processes that are tightly regulated by calcium. Similarly, in lymphocytes, such calcium-dependent membrane targeting is a critical step in signal transduction following antigen receptor engagement. ## Research Directions The dual-lineage expression of [CPNE7](/details-gene/27132) in both the immune and nervous systems raises important questions about its specific roles in these distinct contexts. While its fundamental biochemical function as a calcium-dependent membrane-binding protein is established, its precise physiological substrates and downstream effects remain to be elucidated. **Proposed Hypotheses:** 1. Given its high significance in multiple cortical interneuron subtypes ([sst GABAergic cortical interneuron](/details-cell/CL4023017), [VIP GABAergic cortical interneuron](/details-cell/CL4023016)) and its annotated localization to the [synapse](/details-go/GO:0045202), [CPNE7](/details-gene/27132) may function as a calcium sensor that regulates synaptic vesicle docking or fusion, thereby modulating the strength and kinetics of inhibitory neurotransmission in the cortex. 2. In the immune system, its high expression in [group 3 innate lymphoid cell](/details-cell/CL0001071) and effector T cells suggests [CPNE7](/details-gene/27132) may be a critical mediator of cytokine release. Following receptor-mediated calcium influx, [CPNE7](/details-gene/27132) could facilitate the trafficking and fusion of cytokine-containing vesicles with the plasma membrane. **Key Experimental Approach:** To test the hypothesis regarding its role in inhibitory neurotransmission (Hypothesis 1), a powerful approach would be to generate a conditional knockout mouse model where [CPNE7](/details-gene/27132) is specifically deleted in GABAergic interneurons (e.g., using a GAD2-Cre driver line). Brain slices from these mice could be subjected to electrophysiological analysis (whole-cell patch-clamp) to measure parameters such as inhibitory postsynaptic current (IPSC) amplitude and frequency, and paired-pulse ratio to assess changes in presynaptic release probability. Furthermore, immunofluorescence and super-resolution microscopy could be used to examine synaptic structure and the localization of other key presynaptic proteins in the absence of [CPNE7](/details-gene/27132). **Therapeutic Potential:** The widespread expression of [CPNE7](/details-gene/27132) in both the central nervous system and the immune system makes it a challenging therapeutic target for systemic administration due to the high risk of off-target effects. As an intracellular protein primarily involved in fundamental calcium signaling, targeting it with conventional biologics like antibodies would be difficult. However, if specific gain-of-function or loss-of-function mutations in [CPNE7](/details-gene/27132) are linked to neuroinflammatory or autoimmune diseases, developing highly specific small molecule inhibitors or stabilizers that modulate its lipid-binding or protein-interaction domains could be a potential long-term strategy. Inhibition would likely be the goal if its activity contributes to excessive inflammation or neuronal hyperexcitability.

Genular Protein ID: 988187264

Symbol: CPNE7_HUMAN

Name: N/A

UniProtKB Accession Codes:

Q9UBL6

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 2 CDD 3 CTD 1 ChEBI 9 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 15 Ensembl 2 ExpressionAtlas 1 GO 8 Gene3D 2 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 7 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 2 Pumba 1 RNAct 1 Reactome 1 RefSeq 2 SMART 2 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 10534407

Title: Characterization of copine VII, a new member of the copine family, and its exclusion as a candidate in sporadic breast cancers with loss of heterozygosity at 16q24.3.

PubMed ID: 10534407

DOI: 10.1006/geno.1999.5958

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 12949241

Title: Tissue expression of copines and isolation of copines I and III from the cytosol of human neutrophils.

PubMed ID: 12949241

DOI: 10.1189/jlb.0203083

PubMed ID: 21087455

Title: Copines-1, -2, -3, -6 and -7 show different calcium-dependent intracellular membrane translocation and targeting.

PubMed ID: 21087455

DOI: 10.1111/j.1742-4658.2010.07935.x

Sequence Information:

  • Length: 633
  • Mass: 70294
  • Checksum: 8AF4B68D3EFC51BB
  • Sequence:
    • MSAGSERGAA ATPGGLPAPC ASKVELRLSC RHLLDRDPLT KSDPSVALLQ QAQGQWVQVG 
RTEVVRSSLH PVFSKVFTVD YYFEEVQRLR FEVYDTHGPS GFSCQEDDFL GGMECTLGQP 
AQKWLLQVVM RVSVDVLGPA GHCAKHFLCC TESSHLARTG PSFLLRYDDL CLPWATAGAV 
RWWTCRGGHT QGWQIVAQKK VTRPLLLKFG RNAGKSTITV IAEDISGNNG YVELSFRARK 
LDDKDLFSKS DPFLELYRVN DDQGLQLVYR TEVVKNNLNP VWEAFKVSLS SLCSCEETRP 
LKCLVWDYDS RGKHDFIGEF STTFEEMQKA FEEGQAQWDC VNPKYKQKRR SYKNSGVVVL 
ADLKFHRVYS FLDYIMGGCQ IHFTVAIDFT ASNGDPRNSC SLHYINPYQP NEYLKALVSV 
GEICQDYDSD KRFSALGFGA RIPPKYEVSH DFAINFNPED DECEGIQGVV EAYQNCLPRV 
QLYGPTNVAP IISKVARVAA AEESTGKASQ YYILLILTDG VVTDMADTRE AIVRASRLPM 
SIIIVGVGNA DFTDMQVLDG DDGVLRSPRG EPALRDIVQF VPFRELKNAS PAALAKCVLA 
EVPKQVVEYY SHRGLPPRSL GVPAGEASPG CTP