Details for: BRF1

Gene ID: 2972

Symbol: BRF1

Ensembl ID: ENSG00000185024

Description: BRF1 RNA polymerase III transcription initiation factor subunit

Associated with

  • Gene expression (transcription)
    (R-HSA-74160)
  • Rna polymerase iii abortive and retractive initiation
    (R-HSA-749476)
  • Rna polymerase iii transcription
    (R-HSA-74158)
  • Rna polymerase iii transcription initiation
    (R-HSA-76046)
  • Rna polymerase iii transcription initiation from type 1 promoter
    (R-HSA-76061)
  • Rna polymerase iii transcription initiation from type 2 promoter
    (R-HSA-76066)
  • Dna-templated transcription initiation
    (GO:0006352)
  • Metal ion binding
    (GO:0046872)
  • Nucleoplasm
    (GO:0005654)
  • Nucleus
    (GO:0005634)
  • Positive regulation of transcription by rna polymerase iii
    (GO:0045945)
  • Protein binding
    (GO:0005515)
  • Rna polymerase iii general transcription initiation factor activity
    (GO:0000995)
  • Rna polymerase iii type 3 promoter sequence-specific dna binding
    (GO:0001006)
  • Rrna transcription
    (GO:0009303)
  • Tbp-class protein binding
    (GO:0017025)
  • Transcription by rna polymerase iii
    (GO:0006383)
  • Transcription factor tfiiib complex
    (GO:0000126)
  • Transcription initiation at rna polymerase iii promoter
    (GO:0006384)
  • Transcription preinitiation complex
    (GO:0097550)
  • Transcription preinitiation complex assembly
    (GO:0070897)
  • Trna transcription
    (GO:0009304)

Cells (max top 100)

(Marker Scores and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: kidney proximal straight tubule epithelial cell (CL1000839)
    Fold Change: 1.47
    Marker Score: 3,471
  • Cell Name: immature innate lymphoid cell (CL0001082)
    Fold Change: 1.41
    Marker Score: 2,870
  • Cell Name: neuron (CL0000540)
    Fold Change: 1.33
    Marker Score: 5,416
  • Cell Name: vip GABAergic cortical interneuron (CL4023016)
    Fold Change: 1.33
    Marker Score: 50,459
  • Cell Name: near-projecting glutamatergic cortical neuron (CL4023012)
    Fold Change: 1.31
    Marker Score: 12,347
  • Cell Name: corticothalamic-projecting glutamatergic cortical neuron (CL4023013)
    Fold Change: 1.31
    Marker Score: 12,400
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 1.3
    Marker Score: 79,791
  • Cell Name: caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: 1.28
    Marker Score: 4,965
  • Cell Name: kidney capillary endothelial cell (CL1000892)
    Fold Change: 1.28
    Marker Score: 401
  • Cell Name: L6b glutamatergic cortical neuron (CL4023038)
    Fold Change: 1.27
    Marker Score: 10,949
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: 1.27
    Marker Score: 19,898
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: 1.26
    Marker Score: 12,977
  • Cell Name: lamp5 GABAergic cortical interneuron (CL4023011)
    Fold Change: 1.23
    Marker Score: 18,343
  • Cell Name: pvalb GABAergic cortical interneuron (CL4023018)
    Fold Change: 1.22
    Marker Score: 45,056
  • Cell Name: sncg GABAergic cortical interneuron (CL4023015)
    Fold Change: 1.21
    Marker Score: 9,308
  • Cell Name: mononuclear cell (CL0000842)
    Fold Change: 1.19
    Marker Score: 383
  • Cell Name: L5 extratelencephalic projecting glutamatergic cortical neuron (CL4023041)
    Fold Change: 1.19
    Marker Score: 1,831
  • Cell Name: sst GABAergic cortical interneuron (CL4023017)
    Fold Change: 1.17
    Marker Score: 23,347
  • Cell Name: lymphoid lineage restricted progenitor cell (CL0000838)
    Fold Change: 1.12
    Marker Score: 672
  • Cell Name: germ cell (CL0000586)
    Fold Change: 1.06
    Marker Score: 1,844
  • Cell Name: chandelier pvalb GABAergic cortical interneuron (CL4023036)
    Fold Change: 1.05
    Marker Score: 4,363
  • Cell Name: cerebral cortex GABAergic interneuron (CL0010011)
    Fold Change: 1
    Marker Score: 71,742
  • Cell Name: forebrain radial glial cell (CL0013000)
    Fold Change: 1
    Marker Score: 47,966
  • Cell Name: absorptive cell (CL0000212)
    Fold Change: 0.98
    Marker Score: 30,400
  • Cell Name: corneal epithelial cell (CL0000575)
    Fold Change: 0.97
    Marker Score: 1,070
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 0.95
    Marker Score: 2,404
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 0.94
    Marker Score: 483
  • Cell Name: BEST4+ intestinal epithelial cell, human (CL4030026)
    Fold Change: 0.93
    Marker Score: 437
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: 0.91
    Marker Score: 2,717
  • Cell Name: fibroblast of cardiac tissue (CL0002548)
    Fold Change: 0.88
    Marker Score: 5,290
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.87
    Marker Score: 5,294
  • Cell Name: transit amplifying cell (CL0009010)
    Fold Change: 0.87
    Marker Score: 4,946
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.86
    Marker Score: 308
  • Cell Name: astrocyte of the cerebral cortex (CL0002605)
    Fold Change: 0.85
    Marker Score: 18,151
  • Cell Name: interneuron (CL0000099)
    Fold Change: 0.84
    Marker Score: 382
  • Cell Name: mesangial cell (CL0000650)
    Fold Change: 0.81
    Marker Score: 973
  • Cell Name: intestinal epithelial cell (CL0002563)
    Fold Change: 0.78
    Marker Score: 1,265
  • Cell Name: OFF retinal ganglion cell (CL4023033)
    Fold Change: 0.78
    Marker Score: 326
  • Cell Name: secondary lens fiber (CL0002225)
    Fold Change: 0.77
    Marker Score: 453
  • Cell Name: early T lineage precursor (CL0002425)
    Fold Change: 0.76
    Marker Score: 580
  • Cell Name: smooth muscle myoblast (CL0000514)
    Fold Change: 0.76
    Marker Score: 361
  • Cell Name: oligodendrocyte (CL0000128)
    Fold Change: 0.74
    Marker Score: 1,774
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.72
    Marker Score: 291
  • Cell Name: subcutaneous fat cell (CL0002521)
    Fold Change: 0.72
    Marker Score: 265
  • Cell Name: Cajal-Retzius cell (CL0000695)
    Fold Change: 0.72
    Marker Score: 375
  • Cell Name: epicardial adipocyte (CL1000309)
    Fold Change: 0.7
    Marker Score: 342
  • Cell Name: lens fiber cell (CL0011004)
    Fold Change: 0.7
    Marker Score: 222
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.7
    Marker Score: 2,928
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: 0.7
    Marker Score: 2,921
  • Cell Name: renal principal cell (CL0005009)
    Fold Change: 0.7
    Marker Score: 537
  • Cell Name: corneal endothelial cell (CL0000132)
    Fold Change: 0.68
    Marker Score: 395
  • Cell Name: non-pigmented ciliary epithelial cell (CL0002304)
    Fold Change: 0.68
    Marker Score: 198
  • Cell Name: lens epithelial cell (CL0002224)
    Fold Change: 0.68
    Marker Score: 818
  • Cell Name: brush cell (CL0002204)
    Fold Change: 0.67
    Marker Score: 615
  • Cell Name: inhibitory interneuron (CL0000498)
    Fold Change: 0.67
    Marker Score: 3,107
  • Cell Name: neutrophil progenitor cell (CL0000834)
    Fold Change: 0.66
    Marker Score: 170
  • Cell Name: connective tissue cell (CL0002320)
    Fold Change: 0.65
    Marker Score: 169
  • Cell Name: vascular leptomeningeal cell (CL4023051)
    Fold Change: 0.65
    Marker Score: 748
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: 0.65
    Marker Score: 1,425
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: 0.64
    Marker Score: 634
  • Cell Name: fat cell (CL0000136)
    Fold Change: 0.62
    Marker Score: 345
  • Cell Name: ON retinal ganglion cell (CL4023032)
    Fold Change: 0.61
    Marker Score: 168
  • Cell Name: pyramidal neuron (CL0000598)
    Fold Change: 0.61
    Marker Score: 1,032
  • Cell Name: kidney loop of Henle thin descending limb epithelial cell (CL1001111)
    Fold Change: 0.61
    Marker Score: 654
  • Cell Name: ciliated cell (CL0000064)
    Fold Change: 0.6
    Marker Score: 2,072
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: 0.6
    Marker Score: 807
  • Cell Name: A2 amacrine cell (CL0004219)
    Fold Change: 0.59
    Marker Score: 186
  • Cell Name: kidney interstitial fibroblast (CL1000692)
    Fold Change: 0.58
    Marker Score: 1,111
  • Cell Name: glandular epithelial cell (CL0000150)
    Fold Change: 0.57
    Marker Score: 1,406
  • Cell Name: astrocyte (CL0000127)
    Fold Change: 0.57
    Marker Score: 493
  • Cell Name: cerebellar granule cell precursor (CL0002362)
    Fold Change: 0.57
    Marker Score: 318
  • Cell Name: GABAergic interneuron (CL0011005)
    Fold Change: 0.56
    Marker Score: 216
  • Cell Name: neuronal receptor cell (CL0000006)
    Fold Change: 0.56
    Marker Score: 247
  • Cell Name: Purkinje cell (CL0000121)
    Fold Change: 0.55
    Marker Score: 18,629
  • Cell Name: cortical thymic epithelial cell (CL0002364)
    Fold Change: 0.55
    Marker Score: 2,029
  • Cell Name: kidney connecting tubule epithelial cell (CL1000768)
    Fold Change: 0.54
    Marker Score: 765
  • Cell Name: precursor B cell (CL0000817)
    Fold Change: 0.54
    Marker Score: 356
  • Cell Name: podocyte (CL0000653)
    Fold Change: 0.54
    Marker Score: 198
  • Cell Name: intestinal enteroendocrine cell (CL1001516)
    Fold Change: 0.54
    Marker Score: 427
  • Cell Name: basal cell (CL0000646)
    Fold Change: 0.53
    Marker Score: 686
  • Cell Name: ciliated columnar cell of tracheobronchial tree (CL0002145)
    Fold Change: 0.53
    Marker Score: 4,587
  • Cell Name: common lymphoid progenitor (CL0000051)
    Fold Change: 0.53
    Marker Score: 358
  • Cell Name: mature astrocyte (CL0002627)
    Fold Change: 0.52
    Marker Score: 345
  • Cell Name: proerythroblast (CL0000547)
    Fold Change: 0.52
    Marker Score: 354
  • Cell Name: peripheral nervous system neuron (CL2000032)
    Fold Change: 0.51
    Marker Score: 578
  • Cell Name: male germ cell (CL0000015)
    Fold Change: 0.51
    Marker Score: 147
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: 0.51
    Marker Score: 575
  • Cell Name: adipocyte of epicardial fat of left ventricle (CL1000311)
    Fold Change: 0.51
    Marker Score: 133
  • Cell Name: Leydig cell (CL0000178)
    Fold Change: 0.5
    Marker Score: 539
  • Cell Name: choroid plexus epithelial cell (CL0000706)
    Fold Change: 0.5
    Marker Score: 459
  • Cell Name: cerebral cortex endothelial cell (CL1001602)
    Fold Change: 0.49
    Marker Score: 295
  • Cell Name: smooth muscle cell of sphincter of pupil (CL0002243)
    Fold Change: 0.49
    Marker Score: 156
  • Cell Name: lung endothelial cell (CL1001567)
    Fold Change: 0.49
    Marker Score: 4,296
  • Cell Name: Bergmann glial cell (CL0000644)
    Fold Change: 0.49
    Marker Score: 198
  • Cell Name: acinar cell (CL0000622)
    Fold Change: 0.48
    Marker Score: 341
  • Cell Name: lactocyte (CL0002325)
    Fold Change: 0.48
    Marker Score: 7,730
  • Cell Name: oogonial cell (CL0000024)
    Fold Change: 0.48
    Marker Score: 690
  • Cell Name: leptomeningeal cell (CL0000708)
    Fold Change: 0.47
    Marker Score: 233
  • Cell Name: melanocyte (CL0000148)
    Fold Change: 0.47
    Marker Score: 191
  • Cell Name: pro-B cell (CL0000826)
    Fold Change: 0.47
    Marker Score: 450

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Marker Score to the Marker Score Threshold, indicating how much the gene expression has changed compared to a baseline.
Marker Score: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Marker Score to the Marker Score Threshold, indicating how much the gene expression has changed compared to a baseline.
Marker Score: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Marker Score to the Marker Score Threshold, indicating how much the gene expression has changed compared to a baseline.
Marker Score: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics** 1. **Subunit of RNA polymerase III transcription initiation factor**: BRF1 is a subunit of the RNA polymerase III transcription initiation factor complex, which is essential for the initiation of transcription by RNA polymerase III. 2. **Positive regulation of transcription**: BRF1 enhances the transcriptional activity of RNA polymerase III by facilitating the assembly of the preinitiation complex. 3. **Specific binding to DNA**: BRF1 binds to specific DNA sequences, including type 1 and type 2 promoters, which are recognized by RNA polymerase III. 4. **Metal ion binding**: BRF1 contains metal ion-binding domains, which are essential for its function and stability. 5. **Cell-specific expression**: BRF1 is expressed in various cell types, including kidney proximal straight tubule epithelial cells, immature innate lymphoid cells, neurons, and other cell types. **Pathways and Functions** 1. **Dna-templated transcription initiation**: BRF1 plays a crucial role in the initiation of transcription by RNA polymerase III, which involves the unwinding of DNA and the assembly of the preinitiation complex. 2. **Gene expression (transcription)**: BRF1 regulates the expression of a wide range of genes, including those involved in ribosomal RNA synthesis, tRNA production, and the expression of certain small nuclear RNAs and microRNAs. 3. **RNA polymerase III abortive and retractive initiation**: BRF1 is involved in the regulation of RNA polymerase III abortive and retractive initiation, which is a process that regulates the termination of transcription. 4. **Positive regulation of transcription by RNA polymerase III**: BRF1 enhances the transcriptional activity of RNA polymerase III, which is essential for the expression of certain genes. **Clinical Significance** 1. **Disorders of RNA polymerase III**: Mutations in the BRF1 gene have been associated with disorders of RNA polymerase III, which can lead to developmental abnormalities and other clinical manifestations. 2. **Cancer**: BRF1 has been implicated in the regulation of gene expression in cancer cells, and its dysregulation has been associated with tumorigenesis. 3. **Neurological disorders**: BRF1 has been implicated in the regulation of gene expression in the nervous system, and its dysregulation has been associated with neurological disorders, such as autism and schizophrenia. 4. **Kidney disease**: BRF1 has been implicated in the regulation of gene expression in the kidney, and its dysregulation has been associated with kidney disease. In conclusion, the BRF1 gene plays a crucial role in the regulation of gene expression by facilitating the initiation of transcription by RNA polymerase III. Its dysregulation has been associated with various disorders, including cancer, neurological disorders, and kidney disease. Further research is needed to fully understand the role of BRF1 in human disease and to develop therapeutic strategies for the treatment of BRF1-related disorders.

Genular Protein ID: 3702656121

Symbol: TF3B_HUMAN

Name: Transcription factor IIIB 90 kDa subunit

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 7624363

Title: Structure and function of a human transcription factor TFIIIB subunit that is evolutionarily conserved and contains both TFIIB- and high-mobility-group protein 2-related domains.

PubMed ID: 7624363

DOI: 10.1073/pnas.92.15.7026

PubMed ID: 8943358

Title: RNA polymerase III transcription from the human U6 and adenovirus type 2 VAI promoters has different requirements for human BRF, a subunit of human TFIIIB.

PubMed ID: 8943358

DOI: 10.1128/mcb.16.12.7031

PubMed ID: 10921893

Title: Alternatively spliced hBRF variants function at different RNA polymerase III promoters.

PubMed ID: 10921893

DOI: 10.1093/emboj/19.15.4134

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 12508121

Title: The DNA sequence and analysis of human chromosome 14.

PubMed ID: 12508121

DOI: 10.1038/nature01348

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 14592981

Title: TFIIIB is phosphorylated, disrupted and selectively released from tRNA promoters during mitosis in vivo.

PubMed ID: 14592981

DOI: 10.1093/emboj/cdg544

PubMed ID: 17081983

Title: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.

PubMed ID: 17081983

DOI: 10.1016/j.cell.2006.09.026

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 18377933

Title: Regulation of RNA polymerase III transcription by Maf1 in mammalian cells.

PubMed ID: 18377933

DOI: 10.1016/j.jmb.2008.02.060

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 25561519

Title: BRF1 mutations alter RNA polymerase III-dependent transcription and cause neurodevelopmental anomalies.

PubMed ID: 25561519

DOI: 10.1101/gr.176925.114

PubMed ID: 16959974

Title: The consensus coding sequences of human breast and colorectal cancers.

PubMed ID: 16959974

DOI: 10.1126/science.1133427

Sequence Information:

  • Length: 677
  • Mass: 73840
  • Checksum: 5866304B879EC1DE
  • Sequence:
  • MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL 
    DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGNQL QLNQHCLDTA FNFFKMAVSR 
    HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVNV YVLGKTFLLL ARELCINAPA 
    IDPCLYIPRF AHLLEFGEKN HEVSMTALRL LQRMKRDWMH TGRRPSGLCG AALLVAARMH 
    DFRRTVKEVI SVVKVCESTL RKRLTEFEDT PTSQLTIDEF MKIDLEEECD PPSYTAGQRK 
    LRMKQLEQVL SKKLEEVEGE ISSYQDAIEI ELENSRPKAK GGLASLAKDG STEDTASSLC 
    GEEDTEDEEL EAAASHLNKD LYRELLGGAP GSSEAAGSPE WGGRPPALGS LLDPLPTAAS 
    LGISDSIREC ISSQSSDPKD ASGDGELDLS GIDDLEIDRY ILNESEARVK AELWMRENAE 
    YLREQREKEA RIAKEKELGI YKEHKPKKSC KRREPIQAST AREAIEKMLE QKKISSKINY 
    SVLRGLSSAG GGSPHREDAQ PEHSASARKL SRRRTPASRS GADPVTSVGK RLRPLVSTQP 
    AKKVATGEAL LPSSPTLGAE PARPQAVLVE SGPVSYHADE EADEEEPDEE DGEPCVSALQ 
    MMGSNDYGCD GDEDDGY

Genular Protein ID: 889167371

Symbol: V9HVY2_HUMAN

Name: N/A

UniProtKB Accession Codes:

Database IDs:

Sequence Information:

  • Length: 208
  • Mass: 22309
  • Checksum: 8761AE32A65FFE89
  • Sequence:
  • MTGRVCRGCG GTDIELDAAR GDAVCTACGS VLEDNIIVSE VQFVESSGGG SSAVGQFVSL 
    DGAGKTPTLG GGFHVNLGKE SRAQTLQNGR RHIHHLGNQL QLNQHCLDTA FNFFKMAVSR 
    HLTRGRKMAH VIAACLYLVC RTEGTPHMLL DLSDLLQVDS LRPASFPTWG CDLGVVTRVV 
    TGVYPRCLHA SQWPVCAACP VRKFWSVG

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.