Details for: TOP3A

Gene ID: 7156

Symbol: TOP3A

Ensembl ID: ENSG00000177302

Description: DNA topoisomerase III alpha

Associated with

Cells (max top 100)

(Cell Significance Index and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: polychromatophilic erythroblast (CL0000550)
    Fold Change: 128.9657
    Cell Significance Index: -20.0600
  • Cell Name: hematopoietic oligopotent progenitor cell (CL0002032)
    Fold Change: 80.4673
    Cell Significance Index: -20.4100
  • Cell Name: smooth muscle fiber of ileum (CL1000278)
    Fold Change: 47.2970
    Cell Significance Index: -22.3300
  • Cell Name: mucosal type mast cell (CL0000485)
    Fold Change: 43.2731
    Cell Significance Index: -17.5800
  • Cell Name: ciliated cell of the bronchus (CL0002332)
    Fold Change: 18.4448
    Cell Significance Index: -17.6100
  • Cell Name: orthochromatic erythroblast (CL0000552)
    Fold Change: 17.2512
    Cell Significance Index: -21.2700
  • Cell Name: CD8-alpha-beta-positive, alpha-beta intraepithelial T cell (CL0000796)
    Fold Change: 8.0109
    Cell Significance Index: -21.4600
  • Cell Name: stromal cell of bone marrow (CL0010001)
    Fold Change: 5.4409
    Cell Significance Index: -21.4700
  • Cell Name: CD8-positive, alpha-beta regulatory T cell (CL0000795)
    Fold Change: 4.8186
    Cell Significance Index: -14.8000
  • Cell Name: epidermal Langerhans cell (CL0002457)
    Fold Change: 3.2989
    Cell Significance Index: -7.2200
  • Cell Name: helper T cell (CL0000912)
    Fold Change: 1.0648
    Cell Significance Index: 15.1300
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 1.0208
    Cell Significance Index: 204.7800
  • Cell Name: enteroendocrine cell of colon (CL0009042)
    Fold Change: 0.9859
    Cell Significance Index: 187.6300
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.7312
    Cell Significance Index: 145.1100
  • Cell Name: obsolete caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: 0.6164
    Cell Significance Index: 221.0800
  • Cell Name: retinal progenitor cell (CL0002672)
    Fold Change: 0.5421
    Cell Significance Index: 30.4200
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.4331
    Cell Significance Index: 70.4500
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 0.4206
    Cell Significance Index: 379.7400
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 0.4150
    Cell Significance Index: 45.1400
  • Cell Name: conjunctival epithelial cell (CL1000432)
    Fold Change: 0.3269
    Cell Significance Index: 4.4600
  • Cell Name: fibro/adipogenic progenitor cell (CL0009099)
    Fold Change: 0.2713
    Cell Significance Index: 13.7100
  • Cell Name: epithelial cell of stomach (CL0002178)
    Fold Change: 0.2470
    Cell Significance Index: 28.7900
  • Cell Name: GABAergic amacrine cell (CL4030027)
    Fold Change: 0.2354
    Cell Significance Index: 2.9200
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: 0.2190
    Cell Significance Index: 6.1200
  • Cell Name: indirect pathway medium spiny neuron (CL4023029)
    Fold Change: 0.1694
    Cell Significance Index: 7.5000
  • Cell Name: direct pathway medium spiny neuron (CL4023026)
    Fold Change: 0.1540
    Cell Significance Index: 5.8300
  • Cell Name: colon goblet cell (CL0009039)
    Fold Change: 0.1465
    Cell Significance Index: 14.4900
  • Cell Name: GABAergic interneuron (CL0011005)
    Fold Change: 0.1458
    Cell Significance Index: 100.8200
  • Cell Name: paneth cell of epithelium of small intestine (CL1000343)
    Fold Change: 0.1436
    Cell Significance Index: 3.1100
  • Cell Name: preadipocyte (CL0002334)
    Fold Change: 0.1435
    Cell Significance Index: 2.8000
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.1357
    Cell Significance Index: 3.9100
  • Cell Name: Purkinje cell (CL0000121)
    Fold Change: 0.1334
    Cell Significance Index: 2.9200
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 0.1259
    Cell Significance Index: 8.7100
  • Cell Name: intermediate cell of urothelium (CL4030055)
    Fold Change: 0.1190
    Cell Significance Index: 21.4500
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: 0.1158
    Cell Significance Index: 8.8900
  • Cell Name: stromal cell of ovary (CL0002132)
    Fold Change: 0.1011
    Cell Significance Index: 13.8800
  • Cell Name: eye photoreceptor cell (CL0000287)
    Fold Change: 0.0806
    Cell Significance Index: 5.0800
  • Cell Name: enterocyte of epithelium of large intestine (CL0002071)
    Fold Change: 0.0768
    Cell Significance Index: 3.4800
  • Cell Name: basal cell of urothelium (CL1000486)
    Fold Change: 0.0726
    Cell Significance Index: 8.9300
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 0.0588
    Cell Significance Index: 32.1300
  • Cell Name: hair follicular keratinocyte (CL2000092)
    Fold Change: 0.0574
    Cell Significance Index: 25.3700
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: 0.0431
    Cell Significance Index: 81.1600
  • Cell Name: cerebellar granule cell (CL0001031)
    Fold Change: 0.0414
    Cell Significance Index: 0.7100
  • Cell Name: lens epithelial cell (CL0002224)
    Fold Change: 0.0346
    Cell Significance Index: 53.2500
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: 0.0243
    Cell Significance Index: 44.8900
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 0.0214
    Cell Significance Index: 2.5200
  • Cell Name: non-pigmented ciliary epithelial cell (CL0002304)
    Fold Change: 0.0154
    Cell Significance Index: 9.7700
  • Cell Name: secondary lens fiber (CL0002225)
    Fold Change: 0.0131
    Cell Significance Index: 17.8000
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: 0.0078
    Cell Significance Index: 3.5600
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.0051
    Cell Significance Index: 0.1800
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: -0.0037
    Cell Significance Index: -0.1000
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: -0.0062
    Cell Significance Index: -0.3200
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: -0.0107
    Cell Significance Index: -0.6900
  • Cell Name: acinar cell of salivary gland (CL0002623)
    Fold Change: -0.0155
    Cell Significance Index: -0.7300
  • Cell Name: odontoblast (CL0000060)
    Fold Change: -0.0167
    Cell Significance Index: -2.1400
  • Cell Name: kidney loop of Henle cortical thick ascending limb epithelial cell (CL1001109)
    Fold Change: -0.0189
    Cell Significance Index: -13.8600
  • Cell Name: pigmented ciliary epithelial cell (CL0002303)
    Fold Change: -0.0191
    Cell Significance Index: -2.7700
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: -0.0197
    Cell Significance Index: -14.5800
  • Cell Name: pulmonary alveolar epithelial cell (CL0000322)
    Fold Change: -0.0266
    Cell Significance Index: -20.1100
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: -0.0300
    Cell Significance Index: -16.9400
  • Cell Name: pancreatic PP cell (CL0002275)
    Fold Change: -0.0420
    Cell Significance Index: -26.2300
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: -0.0612
    Cell Significance Index: -17.6200
  • Cell Name: lung endothelial cell (CL1001567)
    Fold Change: -0.0762
    Cell Significance Index: -3.9700
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: -0.0891
    Cell Significance Index: -15.2100
  • Cell Name: intestinal crypt stem cell of small intestine (CL0009017)
    Fold Change: -0.0897
    Cell Significance Index: -1.9100
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: -0.0951
    Cell Significance Index: -9.7100
  • Cell Name: basal cell of prostate epithelium (CL0002341)
    Fold Change: -0.0992
    Cell Significance Index: -2.7000
  • Cell Name: glycinergic neuron (CL1001509)
    Fold Change: -0.1019
    Cell Significance Index: -5.3500
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: -0.1022
    Cell Significance Index: -1.7100
  • Cell Name: pancreatic D cell (CL0000173)
    Fold Change: -0.1101
    Cell Significance Index: -23.1900
  • Cell Name: cortical cell of adrenal gland (CL0002097)
    Fold Change: -0.1142
    Cell Significance Index: -3.0600
  • Cell Name: lactocyte (CL0002325)
    Fold Change: -0.1201
    Cell Significance Index: -15.5100
  • Cell Name: skeletal muscle fiber (CL0008002)
    Fold Change: -0.1253
    Cell Significance Index: -3.2200
  • Cell Name: sebum secreting cell (CL0000317)
    Fold Change: -0.1329
    Cell Significance Index: -9.4000
  • Cell Name: pancreatic ductal cell (CL0002079)
    Fold Change: -0.1372
    Cell Significance Index: -15.7200
  • Cell Name: pvalb GABAergic cortical interneuron (CL4023018)
    Fold Change: -0.1654
    Cell Significance Index: -3.5100
  • Cell Name: luminal adaptive secretory precursor cell of mammary gland (CL4033057)
    Fold Change: -0.1677
    Cell Significance Index: -7.8800
  • Cell Name: smooth muscle cell of sphincter of pupil (CL0002243)
    Fold Change: -0.1685
    Cell Significance Index: -17.5400
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: -0.1786
    Cell Significance Index: -5.7200
  • Cell Name: progenitor cell of mammary luminal epithelium (CL0009116)
    Fold Change: -0.1838
    Cell Significance Index: -13.7000
  • Cell Name: periportal region hepatocyte (CL0019026)
    Fold Change: -0.1988
    Cell Significance Index: -2.9400
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: -0.2023
    Cell Significance Index: -12.4000
  • Cell Name: enteroendocrine cell of small intestine (CL0009006)
    Fold Change: -0.2284
    Cell Significance Index: -5.7100
  • Cell Name: kidney loop of Henle descending limb epithelial cell (CL1001021)
    Fold Change: -0.2434
    Cell Significance Index: -19.2800
  • Cell Name: decidual cell (CL2000002)
    Fold Change: -0.2499
    Cell Significance Index: -4.0100
  • Cell Name: leptomeningeal cell (CL0000708)
    Fold Change: -0.2646
    Cell Significance Index: -5.6600
  • Cell Name: L6b glutamatergic cortical neuron (CL4023038)
    Fold Change: -0.2676
    Cell Significance Index: -8.7600
  • Cell Name: corticothalamic-projecting glutamatergic cortical neuron (CL4023013)
    Fold Change: -0.2707
    Cell Significance Index: -8.6200
  • Cell Name: cardiac muscle cell (CL0000746)
    Fold Change: -0.2716
    Cell Significance Index: -4.0100
  • Cell Name: mesonephric nephron tubule epithelial cell (CL1000022)
    Fold Change: -0.2932
    Cell Significance Index: -10.1900
  • Cell Name: sst GABAergic cortical interneuron (CL4023017)
    Fold Change: -0.3024
    Cell Significance Index: -5.9800
  • Cell Name: OFF midget ganglion cell (CL4033047)
    Fold Change: -0.3456
    Cell Significance Index: -4.3100
  • Cell Name: L5 extratelencephalic projecting glutamatergic cortical neuron (CL4023041)
    Fold Change: -0.3465
    Cell Significance Index: -12.1400
  • Cell Name: lamp5 GABAergic cortical interneuron (CL4023011)
    Fold Change: -0.3647
    Cell Significance Index: -7.8800
  • Cell Name: VIP GABAergic cortical interneuron (CL4023016)
    Fold Change: -0.3676
    Cell Significance Index: -7.3800
  • Cell Name: CD14-positive, CD16-negative classical monocyte (CL0002057)
    Fold Change: -0.3879
    Cell Significance Index: -7.1700
  • Cell Name: near-projecting glutamatergic cortical neuron (CL4023012)
    Fold Change: -0.4051
    Cell Significance Index: -10.1100
  • Cell Name: fibroblast of dermis (CL0002551)
    Fold Change: -0.4142
    Cell Significance Index: -8.6700
  • Cell Name: ON midget ganglion cell (CL4033046)
    Fold Change: -0.4247
    Cell Significance Index: -5.3600
  • Cell Name: chandelier pvalb GABAergic cortical interneuron (CL4023036)
    Fold Change: -0.4331
    Cell Significance Index: -9.0400

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics** TOP3A exhibits the following key characteristics: 1. **Single-strand cutting activity**: Unlike other topoisomerases, TOP3A exhibits single-strand cutting activity, which is essential for its role in DNA replication and repair. 2. **ATP-independent activity**: TOP3A functions in the absence of ATP, making it distinct from other topoisomerases that require energy from ATP hydrolysis. 3. **DNA topological change**: TOP3A is involved in the relaxation of topological stress in DNA, which is critical for maintaining genomic integrity. 4. **Homologous recombination repair**: TOP3A plays a role in the resolution of homologous recombination intermediates, which is essential for the accurate repair of DNA double-strand breaks. **Pathways and Functions** TOP3A is involved in various cellular pathways, including: 1. **Cell cycle regulation**: TOP3A helps regulate the cell cycle by promoting the proper segregation of chromosomes during mitosis. 2. **DNA double-strand break repair**: TOP3A plays a critical role in the resolution of homologous recombination intermediates, ensuring the accurate repair of DNA double-strand breaks. 3. **Homologous recombination repair**: TOP3A is involved in the homologous recombination repair pathway, which is essential for maintaining genomic stability. 4. **DNA replication**: TOP3A helps regulate DNA replication by promoting the proper resolution of topological stress during DNA replication. **Clinical Significance** Dysregulation of TOP3A has been implicated in various human diseases, including: 1. **Cancer**: TOP3A mutations have been identified in various types of cancer, including breast, ovarian, and brain tumors. 2. **Neurodegenerative disorders**: TOP3A has been implicated in neurodegenerative disorders, such as Alzheimer's disease and Parkinson's disease. 3. **Neurodevelopmental disorders**: TOP3A has been implicated in neurodevelopmental disorders, such as autism and schizophrenia. In conclusion, DNA topoisomerase III alpha (TOP3A) is a crucial enzyme involved in maintaining genomic stability through its role in DNA replication, repair, and recombination. Dysregulation of TOP3A has been implicated in various human diseases, highlighting the importance of this enzyme in maintaining cellular homeostasis. Further research is necessary to fully understand the mechanisms by which TOP3A dysregulation contributes to disease and to explore potential therapeutic strategies for targeting this enzyme in the treatment of human diseases.

Genular Protein ID: 2629746850

Symbol: TOP3A_HUMAN

Name: DNA topoisomerase 3-alpha

UniProtKB Accession Codes:

Q13472 A8KA61 B4DK80 D3DXC7 Q13473

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 2 CORUM 1 CTD 1 ChEBI 9 ChiTaRS 1 ComplexPortal 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 EC 1 EMBL 49 Ensembl 4 EvolutionaryTrace 1 ExpressionAtlas 1 GO 17 Gene3D 5 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 13 KEGG 1 MANE-Select 1 MIM 5 MINT 1 MalaCards 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 Orphanet 1 OrthoDB 1 PANTHER 2 PDB 2 PDBsum 2 PRINTS 1 PRO 1 PROSITE 4 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 4 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 3 Pumba 1 RNAct 1 Reactome 15 RefSeq 2 SMART 3 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 8622991

Title: Human TOP3: a single-copy gene encoding DNA topoisomerase III.

PubMed ID: 8622991

DOI: 10.1073/pnas.93.8.3653

PubMed ID: 20843780

Title: Identification of rare DNA variants in mitochondrial disorders with improved array-based sequencing.

PubMed ID: 20843780

DOI: 10.1093/nar/gkq750

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 16625196

Title: DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.

PubMed ID: 16625196

DOI: 10.1038/nature04689

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 15775963

Title: BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity.

PubMed ID: 15775963

DOI: 10.1038/sj.emboj.7600622

PubMed ID: 18923082

Title: RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability.

PubMed ID: 18923082

DOI: 10.1101/gad.1708608

PubMed ID: 18923083

Title: BLAP18/RMI2, a novel OB-fold-containing protein, is an essential component of the Bloom helicase-double Holliday junction dissolvasome.

PubMed ID: 18923083

DOI: 10.1101/gad.1725108

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 20445207

Title: Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1.

PubMed ID: 20445207

DOI: 10.1074/jbc.m110.123216

PubMed ID: 23509288

Title: Scaffolding protein SPIDR/KIAA0146 connects the Bloom syndrome helicase with homologous recombination repair.

PubMed ID: 23509288

DOI: 10.1073/pnas.1220921110

PubMed ID: 30057030

Title: Mutations in TOP3A cause a Bloom syndrome-like disorder.

PubMed ID: 30057030

DOI: 10.1016/j.ajhg.2018.07.001

PubMed ID: 29290614

Title: Topoisomerase 3alpha is required for decatenation and segregation of human mtDNA.

PubMed ID: 29290614

DOI: 10.1016/j.molcel.2017.11.033

Sequence Information:

  • Length: 1001
  • Mass: 112372
  • Checksum: 06558C749569E0C2
  • Sequence:
    • MIFPVARYAL RWLRRPEDRA FSRAAMEMAL RGVRKVLCVA EKNDAAKGIA DLLSNGRMRR 
REGLSKFNKI YEFDYHLYGQ NVTMVMTSVS GHLLAHDFQM QFRKWQSCNP LVLFEAEIEK 
YCPENFVDIK KTLERETRQC QALVIWTDCD REGENIGFEI IHVCKAVKPN LQVLRARFSE 
ITPHAVRTAC ENLTEPDQRV SDAVDVRQEL DLRIGAAFTR FQTLRLQRIF PEVLAEQLIS 
YGSCQFPTLG FVVERFKAIQ AFVPEIFHRI KVTHDHKDGI VEFNWKRHRL FNHTACLVLY 
QLCVEDPMAT VVEVRSKPKS KWRPQALDTV ELEKLASRKL RINAKETMRI AEKLYTQGYI 
SYPRTETNIF PRDLNLTVLV EQQTPDPRWG AFAQSILERG GPTPRNGNKS DQAHPPIHPT 
KYTNNLQGDE QRLYEFIVRH FLACCSQDAQ GQETTVEIDI AQERFVAHGL MILARNYLDV 
YPYDHWSDKI LPVYEQGSHF QPSTVEMVDG ETSPPKLLTE ADLIALMEKH GIGTDATHAE 
HIETIKARMY VGLTPDKRFL PGHLGMGLVE GYDSMGYEMS KPDLRAELEA DLKLICDGKK 
DKFVVLRQQV QKYKQVFIEA VAKAKKLDEA LAQYFGNGTE LAQQEDIYPA MPEPIRKCPQ 
CNKDMVLKTK KNGGFYLSCM GFPECRSAVW LPDSVLEASR DSSVCPVCQP HPVYRLKLKF 
KRGSLPPTMP LEFVCCIGGC DDTLREILDL RFSGGPPRAS QPSGRLQANQ SLNRMDNSQH 
PQPADSRQTG SSKALAQTLP PPTAAGESNS VTCNCGQEAV LLTVRKEGPN RGRQFFKCNG 
GSCNFFLWAD SPNPGAGGPP ALAYRPLGAS LGCPPGPGIH LGGFGNPGDG SGSGTSCLCS 
QPSVTRTVQK DGPNKGRQFH TCAKPREQQC GFFQWVDENT APGTSGAPSW TGDRGRTLES 
EARSKRPRAS SSDMGSTAKK PRKCSLCHQP GHTRPFCPQN R

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.