Details for: TOP3A

Gene ID: 7156

Symbol: TOP3A

Ensembl ID: ENSG00000177302

Description: DNA topoisomerase III alpha

Associated with

Cells (max top 100)

(Marker Score score is uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 1.27
    Marker Score: 78289.5
  • Cell Name: corticothalamic-projecting glutamatergic cortical neuron (CL4023013)
    Fold Change: 1.25
    Marker Score: 11899
  • Cell Name: L6b glutamatergic cortical neuron (CL4023038)
    Fold Change: 1.23
    Marker Score: 10568.5
  • Cell Name: near-projecting glutamatergic cortical neuron (CL4023012)
    Fold Change: 1.22
    Marker Score: 11510
  • Cell Name: L5 extratelencephalic projecting glutamatergic cortical neuron (CL4023041)
    Fold Change: 1.18
    Marker Score: 1829
  • Cell Name: pvalb GABAergic cortical interneuron (CL4023018)
    Fold Change: 1.15
    Marker Score: 42513.5
  • Cell Name: neural progenitor cell (CL0011020)
    Fold Change: 1.13
    Marker Score: 4393
  • Cell Name: caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: 1.13
    Marker Score: 4381
  • Cell Name: OFF retinal ganglion cell (CL4023033)
    Fold Change: 1.1
    Marker Score: 459
  • Cell Name: neuron (CL0000540)
    Fold Change: 1.08
    Marker Score: 4392
  • Cell Name: sst GABAergic cortical interneuron (CL4023017)
    Fold Change: 1.08
    Marker Score: 21418
  • Cell Name: vip GABAergic cortical interneuron (CL4023016)
    Fold Change: 1.04
    Marker Score: 39382.5
  • Cell Name: lamp5 GABAergic cortical interneuron (CL4023011)
    Fold Change: 1.01
    Marker Score: 15128
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: 1.01
    Marker Score: 10446
  • Cell Name: sncg GABAergic cortical interneuron (CL4023015)
    Fold Change: 1
    Marker Score: 7677
  • Cell Name: cerebral cortex GABAergic interneuron (CL0010011)
    Fold Change: 1
    Marker Score: 71758
  • Cell Name: forebrain radial glial cell (CL0013000)
    Fold Change: 1
    Marker Score: 47982
  • Cell Name: chandelier pvalb GABAergic cortical interneuron (CL4023036)
    Fold Change: 0.99
    Marker Score: 4119
  • Cell Name: absorptive cell (CL0000212)
    Fold Change: 0.98
    Marker Score: 30401
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 0.95
    Marker Score: 2405
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 0.94
    Marker Score: 486
  • Cell Name: BEST4+ intestinal epithelial cell, human (CL4030026)
    Fold Change: 0.93
    Marker Score: 440
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: 0.91
    Marker Score: 2721
  • Cell Name: mesangial cell (CL0000650)
    Fold Change: 0.9
    Marker Score: 1084
  • Cell Name: transit amplifying cell (CL0009010)
    Fold Change: 0.88
    Marker Score: 5010
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.87
    Marker Score: 5295
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.86
    Marker Score: 309
  • Cell Name: mononuclear cell (CL0000842)
    Fold Change: 0.82
    Marker Score: 263
  • Cell Name: ON retinal ganglion cell (CL4023032)
    Fold Change: 0.82
    Marker Score: 223
  • Cell Name: intestinal epithelial cell (CL0002563)
    Fold Change: 0.78
    Marker Score: 1259
  • Cell Name: early T lineage precursor (CL0002425)
    Fold Change: 0.74
    Marker Score: 568.5
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.73
    Marker Score: 294
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.73
    Marker Score: 3050
  • Cell Name: kidney proximal straight tubule epithelial cell (CL1000839)
    Fold Change: 0.73
    Marker Score: 1712
  • Cell Name: Cajal-Retzius cell (CL0000695)
    Fold Change: 0.73
    Marker Score: 376
  • Cell Name: lens epithelial cell (CL0002224)
    Fold Change: 0.72
    Marker Score: 871
  • Cell Name: choroid plexus epithelial cell (CL0000706)
    Fold Change: 0.72
    Marker Score: 657.5
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: 0.71
    Marker Score: 2961
  • Cell Name: secondary lens fiber (CL0002225)
    Fold Change: 0.71
    Marker Score: 413
  • Cell Name: brush cell (CL0002204)
    Fold Change: 0.67
    Marker Score: 614
  • Cell Name: proerythroblast (CL0000547)
    Fold Change: 0.67
    Marker Score: 456
  • Cell Name: neutrophil progenitor cell (CL0000834)
    Fold Change: 0.66
    Marker Score: 170
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: 0.63
    Marker Score: 1393.5
  • Cell Name: interneuron (CL0000099)
    Fold Change: 0.63
    Marker Score: 289
  • Cell Name: GABAergic neuron (CL0000617)
    Fold Change: 0.63
    Marker Score: 2633
  • Cell Name: cerebellar granule cell precursor (CL0002362)
    Fold Change: 0.63
    Marker Score: 353
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: 0.6
    Marker Score: 803
  • Cell Name: precursor B cell (CL0000817)
    Fold Change: 0.58
    Marker Score: 382
  • Cell Name: inhibitory interneuron (CL0000498)
    Fold Change: 0.58
    Marker Score: 2668
  • Cell Name: corneal epithelial cell (CL0000575)
    Fold Change: 0.57
    Marker Score: 632
  • Cell Name: astrocyte of the cerebral cortex (CL0002605)
    Fold Change: 0.56
    Marker Score: 11912
  • Cell Name: A2 amacrine cell (CL0004219)
    Fold Change: 0.55
    Marker Score: 175
  • Cell Name: kidney capillary endothelial cell (CL1000892)
    Fold Change: 0.55
    Marker Score: 172
  • Cell Name: epicardial adipocyte (CL1000309)
    Fold Change: 0.55
    Marker Score: 265
  • Cell Name: intestinal enteroendocrine cell (CL1001516)
    Fold Change: 0.55
    Marker Score: 435
  • Cell Name: vascular leptomeningeal cell (CL4023051)
    Fold Change: 0.53
    Marker Score: 615
  • Cell Name: primitive red blood cell (CL0002355)
    Fold Change: 0.52
    Marker Score: 393
  • Cell Name: pigmented ciliary epithelial cell (CL0002303)
    Fold Change: 0.52
    Marker Score: 178
  • Cell Name: ependymal cell (CL0000065)
    Fold Change: 0.5
    Marker Score: 176
  • Cell Name: primordial germ cell (CL0000670)
    Fold Change: 0.49
    Marker Score: 617
  • Cell Name: intestinal crypt stem cell of small intestine (CL0009017)
    Fold Change: 0.49
    Marker Score: 329
  • Cell Name: corneal endothelial cell (CL0000132)
    Fold Change: 0.48
    Marker Score: 282
  • Cell Name: Bergmann glial cell (CL0000644)
    Fold Change: 0.48
    Marker Score: 194.5
  • Cell Name: Purkinje cell (CL0000121)
    Fold Change: 0.47
    Marker Score: 15894.5
  • Cell Name: pulmonary interstitial fibroblast (CL0002241)
    Fold Change: 0.47
    Marker Score: 373
  • Cell Name: non-pigmented ciliary epithelial cell (CL0002304)
    Fold Change: 0.47
    Marker Score: 136
  • Cell Name: smooth muscle cell of sphincter of pupil (CL0002243)
    Fold Change: 0.47
    Marker Score: 147
  • Cell Name: kidney interstitial fibroblast (CL1000692)
    Fold Change: 0.47
    Marker Score: 895
  • Cell Name: fat cell (CL0000136)
    Fold Change: 0.46
    Marker Score: 257
  • Cell Name: oogonial cell (CL0000024)
    Fold Change: 0.45
    Marker Score: 652
  • Cell Name: large pre-B-II cell (CL0000957)
    Fold Change: 0.45
    Marker Score: 1225
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: 0.45
    Marker Score: 445
  • Cell Name: plasmablast (CL0000980)
    Fold Change: 0.45
    Marker Score: 595
  • Cell Name: erythroblast (CL0000765)
    Fold Change: 0.44
    Marker Score: 274
  • Cell Name: common myeloid progenitor (CL0000049)
    Fold Change: 0.44
    Marker Score: 116
  • Cell Name: squamous epithelial cell (CL0000076)
    Fold Change: 0.44
    Marker Score: 298
  • Cell Name: cerebral cortex endothelial cell (CL1001602)
    Fold Change: 0.43
    Marker Score: 259
  • Cell Name: pro-B cell (CL0000826)
    Fold Change: 0.43
    Marker Score: 418
  • Cell Name: astrocyte (CL0000127)
    Fold Change: 0.43
    Marker Score: 375
  • Cell Name: melanocyte (CL0000148)
    Fold Change: 0.43
    Marker Score: 175
  • Cell Name: kidney loop of Henle thin descending limb epithelial cell (CL1001111)
    Fold Change: 0.42
    Marker Score: 457
  • Cell Name: migratory enteric neural crest cell (CL0002607)
    Fold Change: 0.41
    Marker Score: 389
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: 0.4
    Marker Score: 127
  • Cell Name: lens fiber cell (CL0011004)
    Fold Change: 0.4
    Marker Score: 127
  • Cell Name: smooth muscle myoblast (CL0000514)
    Fold Change: 0.4
    Marker Score: 190
  • Cell Name: podocyte (CL0000653)
    Fold Change: 0.39
    Marker Score: 145
  • Cell Name: taste receptor cell (CL0000209)
    Fold Change: 0.39
    Marker Score: 336
  • Cell Name: pyramidal neuron (CL0000598)
    Fold Change: 0.39
    Marker Score: 651.5
  • Cell Name: CD8-positive, alpha-beta cytokine secreting effector T cell (CL0000908)
    Fold Change: 0.39
    Marker Score: 167
  • Cell Name: hepatocyte (CL0000182)
    Fold Change: 0.39
    Marker Score: 263
  • Cell Name: neural crest cell (CL0011012)
    Fold Change: 0.38
    Marker Score: 409
  • Cell Name: fibroblast of breast (CL4006000)
    Fold Change: 0.38
    Marker Score: 217
  • Cell Name: CD14-positive, CD16-negative classical monocyte (CL0002057)
    Fold Change: 0.38
    Marker Score: 21091
  • Cell Name: Unknown (CL0000548)
    Fold Change: 0.37
    Marker Score: 273
  • Cell Name: Schwann cell (CL0002573)
    Fold Change: 0.37
    Marker Score: 129
  • Cell Name: skeletal muscle fiber (CL0008002)
    Fold Change: 0.37
    Marker Score: 931
  • Cell Name: basal cell (CL0000646)
    Fold Change: 0.37
    Marker Score: 479
  • Cell Name: fraction A pre-pro B cell (CL0002045)
    Fold Change: 0.37
    Marker Score: 371
  • Cell Name: kidney distal convoluted tubule epithelial cell (CL1000849)
    Fold Change: 0.37
    Marker Score: 388
  • Cell Name: endothelial cell of hepatic sinusoid (CL1000398)
    Fold Change: 0.37
    Marker Score: 80

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Other Information

**Key Characteristics** TOP3A exhibits the following key characteristics: 1. **Single-strand cutting activity**: Unlike other topoisomerases, TOP3A exhibits single-strand cutting activity, which is essential for its role in DNA replication and repair. 2. **ATP-independent activity**: TOP3A functions in the absence of ATP, making it distinct from other topoisomerases that require energy from ATP hydrolysis. 3. **DNA topological change**: TOP3A is involved in the relaxation of topological stress in DNA, which is critical for maintaining genomic integrity. 4. **Homologous recombination repair**: TOP3A plays a role in the resolution of homologous recombination intermediates, which is essential for the accurate repair of DNA double-strand breaks. **Pathways and Functions** TOP3A is involved in various cellular pathways, including: 1. **Cell cycle regulation**: TOP3A helps regulate the cell cycle by promoting the proper segregation of chromosomes during mitosis. 2. **DNA double-strand break repair**: TOP3A plays a critical role in the resolution of homologous recombination intermediates, ensuring the accurate repair of DNA double-strand breaks. 3. **Homologous recombination repair**: TOP3A is involved in the homologous recombination repair pathway, which is essential for maintaining genomic stability. 4. **DNA replication**: TOP3A helps regulate DNA replication by promoting the proper resolution of topological stress during DNA replication. **Clinical Significance** Dysregulation of TOP3A has been implicated in various human diseases, including: 1. **Cancer**: TOP3A mutations have been identified in various types of cancer, including breast, ovarian, and brain tumors. 2. **Neurodegenerative disorders**: TOP3A has been implicated in neurodegenerative disorders, such as Alzheimer's disease and Parkinson's disease. 3. **Neurodevelopmental disorders**: TOP3A has been implicated in neurodevelopmental disorders, such as autism and schizophrenia. In conclusion, DNA topoisomerase III alpha (TOP3A) is a crucial enzyme involved in maintaining genomic stability through its role in DNA replication, repair, and recombination. Dysregulation of TOP3A has been implicated in various human diseases, highlighting the importance of this enzyme in maintaining cellular homeostasis. Further research is necessary to fully understand the mechanisms by which TOP3A dysregulation contributes to disease and to explore potential therapeutic strategies for targeting this enzyme in the treatment of human diseases.

Genular Protein ID: 2629746850

Symbol: TOP3A_HUMAN

Name: DNA topoisomerase 3-alpha

UniProtKB Accession Codes:

Q13472 A8KA61 B4DK80 D3DXC7 Q13473

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 2 CORUM 1 CTD 1 ChEBI 9 ChiTaRS 1 ComplexPortal 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 EC 1 EMBL 49 EPD 1 Ensembl 6 ExpressionAtlas 1 GO 16 Gene3D 5 GeneCards 1 GeneTree 1 GeneWiki 1 Genevisible 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 13 KEGG 1 MANE-Select 1 MIM 5 MINT 1 MalaCards 1 MassIVE 1 MaxQB 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 Orphanet 1 OrthoDB 1 PANTHER 2 PDB 3 PDBsum 2 PRINTS 1 PRO 1 PROSITE 4 PROSITE-ProRule 4 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 4 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 3 Pumba 1 RNAct 1 Reactome 15 RefSeq 2 SAM 1 SMART 3 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 8622991

Title: Human TOP3: a single-copy gene encoding DNA topoisomerase III.

PubMed ID: 8622991

DOI: 10.1073/pnas.93.8.3653

PubMed ID: 20843780

Title: Identification of rare DNA variants in mitochondrial disorders with improved array-based sequencing.

PubMed ID: 20843780

DOI: 10.1093/nar/gkq750

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 16625196

Title: DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.

PubMed ID: 16625196

DOI: 10.1038/nature04689

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 15775963

Title: BLAP75, an essential component of Bloom's syndrome protein complexes that maintain genome integrity.

PubMed ID: 15775963

DOI: 10.1038/sj.emboj.7600622

PubMed ID: 18923082

Title: RMI, a new OB-fold complex essential for Bloom syndrome protein to maintain genome stability.

PubMed ID: 18923082

DOI: 10.1101/gad.1708608

PubMed ID: 18923083

Title: BLAP18/RMI2, a novel OB-fold-containing protein, is an essential component of the Bloom helicase-double Holliday junction dissolvasome.

PubMed ID: 18923083

DOI: 10.1101/gad.1725108

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 20445207

Title: Human topoisomerase IIIalpha is a single-stranded DNA decatenase that is stimulated by BLM and RMI1.

PubMed ID: 20445207

DOI: 10.1074/jbc.m110.123216

PubMed ID: 23509288

Title: Scaffolding protein SPIDR/KIAA0146 connects the Bloom syndrome helicase with homologous recombination repair.

PubMed ID: 23509288

DOI: 10.1073/pnas.1220921110

PubMed ID: 30057030

Title: Mutations in TOP3A cause a Bloom syndrome-like disorder.

PubMed ID: 30057030

DOI: 10.1016/j.ajhg.2018.07.001

PubMed ID: 29290614

Title: Topoisomerase 3alpha is required for decatenation and segregation of human mtDNA.

PubMed ID: 29290614

DOI: 10.1016/j.molcel.2017.11.033

Sequence Information:

  • Length: 1001
  • Mass: 112372
  • Checksum: 06558C749569E0C2
  • Sequence:
    • MIFPVARYAL RWLRRPEDRA FSRAAMEMAL RGVRKVLCVA EKNDAAKGIA DLLSNGRMRR 
REGLSKFNKI YEFDYHLYGQ NVTMVMTSVS GHLLAHDFQM QFRKWQSCNP LVLFEAEIEK 
YCPENFVDIK KTLERETRQC QALVIWTDCD REGENIGFEI IHVCKAVKPN LQVLRARFSE 
ITPHAVRTAC ENLTEPDQRV SDAVDVRQEL DLRIGAAFTR FQTLRLQRIF PEVLAEQLIS 
YGSCQFPTLG FVVERFKAIQ AFVPEIFHRI KVTHDHKDGI VEFNWKRHRL FNHTACLVLY 
QLCVEDPMAT VVEVRSKPKS KWRPQALDTV ELEKLASRKL RINAKETMRI AEKLYTQGYI 
SYPRTETNIF PRDLNLTVLV EQQTPDPRWG AFAQSILERG GPTPRNGNKS DQAHPPIHPT 
KYTNNLQGDE QRLYEFIVRH FLACCSQDAQ GQETTVEIDI AQERFVAHGL MILARNYLDV 
YPYDHWSDKI LPVYEQGSHF QPSTVEMVDG ETSPPKLLTE ADLIALMEKH GIGTDATHAE 
HIETIKARMY VGLTPDKRFL PGHLGMGLVE GYDSMGYEMS KPDLRAELEA DLKLICDGKK 
DKFVVLRQQV QKYKQVFIEA VAKAKKLDEA LAQYFGNGTE LAQQEDIYPA MPEPIRKCPQ 
CNKDMVLKTK KNGGFYLSCM GFPECRSAVW LPDSVLEASR DSSVCPVCQP HPVYRLKLKF 
KRGSLPPTMP LEFVCCIGGC DDTLREILDL RFSGGPPRAS QPSGRLQANQ SLNRMDNSQH 
PQPADSRQTG SSKALAQTLP PPTAAGESNS VTCNCGQEAV LLTVRKEGPN RGRQFFKCNG 
GSCNFFLWAD SPNPGAGGPP ALAYRPLGAS LGCPPGPGIH LGGFGNPGDG SGSGTSCLCS 
QPSVTRTVQK DGPNKGRQFH TCAKPREQQC GFFQWVDENT APGTSGAPSW TGDRGRTLES 
EARSKRPRAS SSDMGSTAKK PRKCSLCHQP GHTRPFCPQN R

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. For the full schema, download it here.