Details for: NELFA

Gene ID: 7469

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: NELFA

Ensembl ID: ENSG00000185049

Description: negative elongation factor complex member A

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 15.04
    rCSI 36.56%
    PRS 57.31
  • stromal cell CL0000499
    CSI 9.55
    rCSI 26.85%
    PRS 72.62
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 9.3
    rCSI 33.47%
    PRS 57.2
  • mesodermal cell CL0000222
    CSI 8.74
    rCSI 10.49%
    PRS 75.24
  • L6b glutamatergic cortical neuron CL4023038
    CSI 8.42
    rCSI 26.32%
    PRS 60.95
  • CD4-positive, CD25-positive, alpha-beta regulatory T cell CL0000792
    CSI 5.39
    rCSI 5.29%
    PRS 90.6
  • retinal rod cell CL0000604
    CSI 5.1
    rCSI 8.99%
    PRS 73.35
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 4.81
    rCSI 18.19%
    PRS 59.75
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 4.65
    rCSI 27.38%
    PRS 60.08
  • type B pancreatic cell CL0000169
    CSI 4.38
    rCSI 9.71%
    PRS 76.81
  • regular ventricular cardiac myocyte CL0002131
    CSI 4
    rCSI 24.97%
    PRS 69.13
  • unswitched memory B cell CL0000970
    CSI 3.9
    rCSI 3.28%
    PRS 90.18
  • melanocyte CL0000148
    CSI 3.69
    rCSI 2.73%
    PRS 70.79
  • effector CD8-positive, alpha-beta T cell CL0001050
    CSI 3.37
    rCSI 2.56%
    PRS 89.58
  • ependymal cell CL0000065
    CSI 3.24
    rCSI 6.57%
    PRS 55.85
  • cerebellar granule cell CL0001031
    CSI 3.23
    rCSI 4.75%
    PRS 70.25
  • mesenchymal cell CL0008019
    CSI 3.17
    rCSI 8.06%
    PRS 70.86
  • epithelial cell of lower respiratory tract CL0002632
    CSI 2.95
    rCSI 2.28%
    PRS 81.12
  • stem cell CL0000034
    CSI 2.9
    rCSI 2.79%
    PRS 70.39
  • perivascular cell CL4033054
    CSI 2.88
    rCSI 3.94%
    PRS 81.98
  • neural crest cell CL0011012
    CSI 2.85
    rCSI 2.25%
    PRS 65.79
  • choroid plexus epithelial cell CL0000706
    CSI 2.84
    rCSI 4.66%
    PRS 66.88
  • plasmacytoid dendritic cell, human CL0001058
    CSI 2.83
    rCSI 1.98%
    PRS 80.79
  • interstitial cell of Cajal CL0002088
    CSI 2.83
    rCSI 3.6%
    PRS 82.58
  • central nervous system neuron CL2000029
    CSI 2.78
    rCSI 20.42%
    PRS 64.6
  • hematopoietic stem cell CL0000037
    CSI 2.74
    rCSI 1.82%
    PRS 80.44
  • mature B cell CL0000785
    CSI 2.67
    rCSI 2.32%
    PRS 86.97
  • Mueller cell CL0000636
    CSI 2.65
    rCSI 6.06%
    PRS 68.95
  • activated CD4-positive, alpha-beta T cell CL0000896
    CSI 2.51
    rCSI 2.32%
    PRS 91.73
  • vascular leptomeningeal cell CL4023051
    CSI 2.45
    rCSI 4.3%
    PRS 70.9
  • precursor B cell CL0000817
    CSI 2.45
    rCSI 2.14%
    PRS 85.02
  • epithelial cell of lung CL0000082
    CSI 2.42
    rCSI 2%
    PRS 78.09
  • intestine goblet cell CL0019031
    CSI 2.37
    rCSI 2.1%
    PRS 75.3
  • CD14-low, CD16-positive monocyte CL0002396
    CSI 2.32
    rCSI 1.78%
    PRS 80.16
  • cerebral cortex endothelial cell CL1001602
    CSI 2.29
    rCSI 3.96%
    PRS 68.76
  • pro-B cell CL0000826
    CSI 2.25
    rCSI 1.86%
    PRS 80.05
  • hepatic stellate cell CL0000632
    CSI 2.22
    rCSI 8.32%
    PRS 69.8
  • direct pathway medium spiny neuron CL4023026
    CSI 2.2
    rCSI 52.64%
    PRS 57.67
  • indirect pathway medium spiny neuron CL4023029
    CSI 2.17
    rCSI 52.38%
    PRS 58.24
  • interneuron CL0000099
    CSI 2.16
    rCSI 4.33%
    PRS 67.32
  • kidney loop of Henle thin descending limb epithelial cell CL1001111
    CSI 2.15
    rCSI 3.05%
    PRS 74.23
  • colon epithelial cell CL0011108
    CSI 2.13
    rCSI 2.23%
    PRS 74.8
  • VIP GABAergic cortical interneuron CL4023016
    CSI 2.11
    rCSI 2.52%
    PRS 59.24
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 2.09
    rCSI 3.68%
    PRS 58.48
  • rod bipolar cell CL0000751
    CSI 2.05
    rCSI 3.69%
    PRS 70.8
  • CD4-positive helper T cell CL0000492
    CSI 2.02
    rCSI 1.53%
    PRS 89.32
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 1.99
    rCSI 2.3%
    PRS 69.79
  • granulocyte monocyte progenitor cell CL0000557
    CSI 1.99
    rCSI 1.72%
    PRS 81.72
  • common dendritic progenitor CL0001029
    CSI 1.96
    rCSI 2.46%
    PRS 86.49
  • Bergmann glial cell CL0000644
    CSI 1.91
    rCSI 2.61%
    PRS 69.53
  • GABAergic neuron CL0000617
    CSI 1.9
    rCSI 6.36%
    PRS 61.56
  • inhibitory interneuron CL0000498
    CSI 1.88
    rCSI 4.33%
    PRS 65.48
  • group 3 innate lymphoid cell CL0001071
    CSI 1.86
    rCSI 1.4%
    PRS 83.38
  • radial glial cell CL0000681
    CSI 1.84
    rCSI 2.56%
    PRS 76.25
  • placental villous trophoblast CL2000060
    CSI 1.83
    rCSI 2.83%
    PRS 76.55
  • glioblast CL0000030
    CSI 1.83
    rCSI 2.92%
    PRS 69.24
  • renal beta-intercalated cell CL0002201
    CSI 1.82
    rCSI 4.34%
    PRS 77.76
  • ciliated columnar cell of tracheobronchial tree CL0002145
    CSI 1.82
    rCSI 4.15%
    PRS 71.65
  • common myeloid progenitor CL0000049
    CSI 1.79
    rCSI 1.44%
    PRS 79.93
  • sst GABAergic cortical interneuron CL4023017
    CSI 1.78
    rCSI 2.3%
    PRS 60.43
  • ciliated epithelial cell CL0000067
    CSI 1.78
    rCSI 1.56%
    PRS 66.41
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 1.78
    rCSI 2.21%
    PRS 57.15
  • retinal bipolar neuron CL0000748
    CSI 1.76
    rCSI 3.3%
    PRS 65.6
  • retina horizontal cell CL0000745
    CSI 1.76
    rCSI 2.69%
    PRS 74.35
  • neural progenitor cell CL0011020
    CSI 1.76
    rCSI 7.74%
    PRS 66.03
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 1.74
    rCSI 2.24%
    PRS 73.77
  • conjunctival epithelial cell CL1000432
    CSI 1.71
    rCSI 2.61%
    PRS 77.81
  • glutamatergic neuron CL0000679
    CSI 1.7
    rCSI 3.49%
    PRS 65.19
  • keratinocyte CL0000312
    CSI 1.68
    rCSI 1.41%
    PRS 80.55
  • differentiation-committed oligodendrocyte precursor CL4023059
    CSI 1.67
    rCSI 3.03%
    PRS 68.86
  • medium spiny neuron CL1001474
    CSI 1.66
    rCSI 14.27%
    PRS 65.23
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 1.63
    rCSI 1.47%
    PRS 75.68
  • L2/3 intratelencephalic projecting glutamatergic neuron CL4030059
    CSI 1.62
    rCSI 3.51%
    PRS 65.05
  • extravillous trophoblast CL0008036
    CSI 1.6
    rCSI 1.99%
    PRS 75.26
  • BEST4+ enteroycte CL4030026
    CSI 1.6
    rCSI 1.99%
    PRS 78.88
  • lung ciliated cell CL1000271
    CSI 1.58
    rCSI 1.82%
    PRS 69.44
  • chondrocyte CL0000138
    CSI 1.52
    rCSI 2.42%
    PRS 70.31
  • parietal epithelial cell CL1000452
    CSI 1.51
    rCSI 4.03%
    PRS 68.92
  • cerebral cortex neuron CL0010012
    CSI 1.49
    rCSI 6.07%
    PRS 69.7
  • neuron CL0000540
    CSI 1.45
    rCSI 3.86%
    PRS 65.37
  • astrocyte of the cerebral cortex CL0002605
    CSI 1.45
    rCSI 3.25%
    PRS 60.03
  • retinal ganglion cell CL0000740
    CSI 1.41
    rCSI 3.1%
    PRS 63.19
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 1.32
    rCSI 2.22%
    PRS 59.27
  • L4 intratelencephalic projecting glutamatergic neuron CL4030063
    CSI 1.3
    rCSI 3.11%
    PRS 64.39
  • intestinal tuft cell CL0019032
    CSI 1.26
    rCSI 1.92%
    PRS 81.51
  • peripheral nervous system neuron CL2000032
    CSI 1.25
    rCSI 1.71%
    PRS 68.95
  • cardiac muscle cell CL0000746
    CSI 1.24
    rCSI 1.79%
    PRS 67.25
  • cardiac neuron CL0010022
    CSI 1.23
    rCSI 3.93%
    PRS 74.6
  • erythrocyte CL0000232
    CSI 1.19
    rCSI 2.71%
    PRS 78.46
  • sncg GABAergic cortical interneuron CL4023015
    CSI 1.18
    rCSI 1.91%
    PRS 60.97
  • chandelier pvalb GABAergic cortical interneuron CL4023036
    CSI 1.04
    rCSI 3.24%
    PRS 63.37
  • retinal cone cell CL0000573
    CSI 1.02
    rCSI 1.65%
    PRS 67.35
  • amacrine cell CL0000561
    CSI 1.01
    rCSI 2.93%
    PRS 66.78
  • L5/6 near-projecting glutamatergic neuron CL4030067
    CSI 1
    rCSI 3.3%
    PRS 63.81
  • CD8-positive, CD28-negative, alpha-beta regulatory T cell CL0000920
    CSI 0.86
    rCSI 1.71%
    PRS 89.62
  • kidney connecting tubule epithelial cell CL1000768
    CSI 0.75
    rCSI 1.9%
    PRS 67.74
  • primitive red blood cell CL0002355
    CSI 0.5
    rCSI 2.71%
    PRS 84.54
  • blood vessel smooth muscle cell CL0019018
    CSI 0.38
    rCSI 3.06%
    PRS 71.36

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [NELFA](/details-gene/7469) (Negative Elongation Factor Complex Member A) is a protein-coding gene located on chromosome 4p16.3. It encodes a core subunit of the Negative Elongation Factor (NELF) complex, a critical regulator of gene expression. The primary function of the NELF complex is to induce promoter-proximal pausing of RNA Polymerase II, thereby providing a key checkpoint in transcriptional elongation. Functional annotations reveal its dual role in both the negative and positive regulation of transcription. **Overall**, expression data indicates that while [NELFA](/details-gene/7469) is broadly expressed, it shows exceptionally high significance in various subtypes of cortical glutamatergic neurons, suggesting a specialized role in the central nervous system. It is also implicated in pathways related to viral life cycles, particularly HIV, and has been linked to the critical region for Wolf-Hirschhorn syndrome [Link](https://doi.org/10.1006/geno.1999.5871). ## Cellular Roles and Expression Landscape The expression profile of [NELFA](/details-gene/7469) points to a fundamental role in transcriptional regulation across diverse cell types, with a pronounced significance in the nervous system. The gene's highest significance scores are observed in a variety of neuronal populations, particularly excitatory neurons of the cerebral cortex. These include [L2/3-6 intratelencephalic projecting glutamatergic neuron](/details-cell/CL4023040) (CSI: 15.04), [L5 extratelencephalic projecting glutamatergic cortical neuron](/details-cell/CL4023041) (CSI: 9.30), and [L6b glutamatergic cortical neuron](/details-cell/CL4023038) (CSI: 8.42). This suggests that precise control of transcriptional pausing via [NELFA](/details-gene/7469) is essential for establishing or maintaining the identity and function of these specific neuronal subtypes. Beyond the nervous system, [NELFA](/details-gene/7469) also demonstrates significant expression in non-neuronal cell types, highlighting its ubiquitous role. It is a notable marker in [stromal cell](/details-cell/CL0000499) (CSI: 9.55) and [mesodermal cell](/details-cell/CL0000222) (CSI: 8.74), indicating importance in connective and developmental tissues. Furthermore, its expression in immune cells, including [CD4-positive, CD25-positive, alpha-beta regulatory T cell](/details-cell/CL0000792) (CSI: 5.39), [unswitched memory B cell](/details-cell/CL0000970) (CSI: 3.90), and [effector CD8-positive, alpha-beta T cell](/details-cell/CL0001050) (CSI: 3.37), is consistent with its role as a host factor in viral infection pathways and general immune cell regulation. ## Pathways and Molecular Function Functionally, [NELFA](/details-gene/7469) is integral to the regulation of eukaryotic gene expression. As a component of the [NELF complex](/details-go/GO:0032021), it participates directly in controlling RNA Polymerase II activity. Gene Ontology annotations confirm its involvement in both '[Negative regulation of transcription elongation by rna polymerase ii](/details-go/GO:0034244)' and '[Positive regulation of transcription by rna polymerase ii](/details-go/GO:0045944)', reflecting the NELF complex's ability to first pause transcription and then facilitate its release upon receiving appropriate signals. Its molecular functions include '[Chromatin binding](/details-go/GO:0003682)' and '[Protein binding](/details-go/GO:0005515)', which are essential for its role within the larger transcriptional machinery. Reactome pathway analysis further elucidates its role, placing it centrally within the '[Gene expression (transcription)](/details-reactome/R-HSA-74160)' and '[Rna polymerase ii transcription elongation](/details-reactome/R-HSA-75955)' pathways. A striking number of associated pathways relate to viral pathogenesis, particularly '[Hiv infection](/details-reactome/R-HSA-162906)' and the '[Formation of hiv elongation complex in the absence of hiv tat](/details-reactome/R-HSA-167152)'. This suggests that viruses like HIV may co-opt [NELFA](/details-gene/7469) and the host's transcriptional pausing machinery to regulate their own life cycles, a concept supported by literature showing NELF's role in viral transcription [Link](https://doi.org/10.1126/science.1057925). Additionally, its inclusion in the '[Transcriptional regulation by tp53](/details-reactome/R-HSA-3700989)' pathway connects it to cellular stress responses and tumor suppression. ## Research Directions The widespread yet cell-type-specific significance of [NELFA](/details-gene/7469) presents several avenues for future investigation, particularly concerning its roles in neuronal function and host-pathogen interactions. **Proposed Hypotheses:** 1. Given its exceptionally high significance in diverse cortical glutamatergic neurons, [NELFA](/details-gene/7469) may be indispensable for fine-tuning the expression of genes critical for neuronal subtype specification, synaptic plasticity, and long-term memory. Its role in pausing RNA Pol II could allow for rapid, coordinated transcriptional responses to neuronal activity. 2. The strong association with HIV-related pathways suggests that [NELFA](/details-gene/7469) could be a key host dependency factor that regulates HIV latency. By controlling the elongation of the integrated provirus, variations in [NELFA](/details-gene/7469) activity within T cells may determine whether the virus remains dormant or enters a productive replication cycle. 3. As [NELFA](/details-gene/7469) is located within the Wolf-Hirschhorn syndrome critical region, it is plausible that haploinsufficiency of this gene contributes to the neurological phenotypes of the syndrome by disrupting the precise dose-dependent regulation of key developmental genes in the embryonic cortex. **Experimental Approach:** To test the hypothesis that [NELFA](/details-gene/7469) is critical for neuronal gene regulation (Hypothesis 1), a compelling experiment would be to use a CRISPR interference (CRISPRi) system to achieve targeted knockdown of [NELFA](/details-gene/7469) in human induced pluripotent stem cell (iPSC)-derived cortical glutamatergic neurons. The impact on transcription could be assessed globally via RNA-sequencing and, more directly, by using techniques like Precision Run-On sequencing (PRO-seq) to measure changes in RNA Polymerase II pausing and elongation rates genome-wide. This would reveal which specific genes and pathways are most sensitive to [NELFA](/details-gene/7469) levels in a neuronal context. **Therapeutic Potential:** As a core component of the general transcription machinery, global targeting of [NELFA](/details-gene/7469) would likely be associated with significant toxicity. However, its crucial role in the HIV life cycle makes it a potential host-directed antiviral target. Small molecule inhibitors designed to disrupt the interaction of [NELFA](/details-gene/7469) with viral proteins or other NELF components could represent a novel strategy to suppress viral replication, potentially complementing existing antiretroviral therapies. Such a strategy would focus on **inhibition** of its function to prevent viral gene expression. Its potential role in neurological disorders is intriguing, but therapeutic modulation would require highly advanced, cell-type-specific delivery mechanisms that are not yet available.

Genular Protein ID: 4281688024

Symbol: NELFA_HUMAN

Name: Negative elongation factor A

UniProtKB Accession Codes:

Q9H3P2 A2A2T1 O95392

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CORUM 1 CTD 1 ChiTaRS 1 ComplexPortal 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 DisProt 1 EMBL 7 EMDB 11 Ensembl 1 ExpressionAtlas 1 GO 10 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 2 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MalaCards 1 MassIVE 1 MetOSite 1 NCBI Taxonomy 1 OpenTargets 1 Orphanet 1 OrthoDB 1 PANTHER 2 PDB 11 PDBsum 11 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 2 Pumba 1 RNAct 1 Reactome 14 RefSeq 1 SIGNOR 1 SMR 1 STRING 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 10409432

Title: Comparative analysis of a novel gene from the Wolf-Hirschhorn/Pitt-Rogers-Danks syndrome critical region.

PubMed ID: 10409432

DOI: 10.1006/geno.1999.5871

PubMed ID: 11280764

Title: Molecular basis of T cell-mediated recognition of pancreatic cancer cells.

PubMed ID: 11280764

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15815621

Title: Generation and annotation of the DNA sequences of human chromosomes 2 and 4.

PubMed ID: 15815621

DOI: 10.1038/nature03466

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 11387440

Title: Stimulation of RNA polymerase II elongation by hepatitis delta antigen.

PubMed ID: 11387440

DOI: 10.1126/science.1057925

PubMed ID: 10199401

Title: NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation.

PubMed ID: 10199401

DOI: 10.1016/s0092-8674(00)80713-8

PubMed ID: 11150502

Title: Modulation of WHSC2 expression in human endothelial cells.

PubMed ID: 11150502

DOI: 10.1016/s0014-5793(00)02335-8

PubMed ID: 12563561

Title: Mapping the Wolf-Hirschhorn syndrome phenotype outside the currently accepted WHS critical region and defining a new critical region, WHSCR-2.

PubMed ID: 12563561

DOI: 10.1086/367925

PubMed ID: 12612062

Title: Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex.

PubMed ID: 12612062

DOI: 10.1128/mcb.23.6.1863-1873.2003

PubMed ID: 16964243

Title: A probability-based approach for high-throughput protein phosphorylation analysis and site localization.

PubMed ID: 16964243

DOI: 10.1038/nbt1240

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 19413330

Title: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.

PubMed ID: 19413330

DOI: 10.1021/ac9004309

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 23884411

Title: Negative elongation factor (NELF) coordinates RNA polymerase II pausing, premature termination, and chromatin remodeling to regulate HIV transcription.

PubMed ID: 23884411

DOI: 10.1074/jbc.m113.496489

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 27282391

Title: Architecture and RNA binding of the human negative elongation factor.

PubMed ID: 27282391

DOI: 10.7554/elife.14981

Sequence Information:

  • Length: 528
  • Mass: 57277
  • Checksum: 4F33C3BC68BDC798
  • Sequence:
    • MASMRESDTG LWLHNKLGAT DELWAPPSIA SLLTAAVIDN IRLCFHGLSS AVKLKLLLGT 
LHLPRRTVDE MKGALMEIIQ LASLDSDPWV LMVADILKSF PDTGSLNLEL EEQNPNVQDI 
LGELREKVGE CEASAMLPLE CQYLNKNALT TLAGPLTPPV KHFQLKRKPK SATLRAELLQ 
KSTETAQQLK RSAGVPFHAK GRGLLRKMDT TTPLKGIPKQ APFRSPTAPS VFSPTGNRTP 
IPPSRTLLRK ERGVKLLDIS ELDMVGAGRE AKRRRKTLDA EVVEKPAKEE TVVENATPDY 
AAGLVSTQKL GSLNNEPALP STSYLPSTPS VVPASSYIPS SETPPAPSSR EASRPPEEPS 
APSPTLPAQF KQRAPMYNSG LSPATPTPAA PTSPLTPTTP PAVAPTTQTP PVAMVAPQTQ 
APAQQQPKKN LSLTREQMFA AQEMFKTANK VTRPEKALIL GFMAGSRENP CQEQGDVIQI 
KLSEHTEDLP KADGQGSTTM LVDTVFEMNY ATGQWTRFKK YKPMTNVS

Genular Protein ID: 3849663971

Symbol: A0A0C4DFX9_HUMAN

Name: N/A

UniProtKB Accession Codes:

A0A0C4DFX9

Database IDs:

AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID-ORCS 1 CTD 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 1 Ensembl 2 ExpressionAtlas 1 GO 3 GeneTree 1 HGNC 1 IntAct 1 InterPro 2 KEGG 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OrthoDB 1 PANTHER 2 PROSITE 2 PeptideAtlas 1 PhylomeDB 1 Proteomes 2 ProteomicsDB 1 RefSeq 1 SAM 1 SMR 1 VEuPathDB 1

Citations:

PubMed ID: 11237011

Title: Initial sequencing and analysis of the human genome.

PubMed ID: 11237011

DOI: 10.1038/35057062

PubMed ID: 15496913

Title: Finishing the euchromatic sequence of the human genome.

PubMed ID: 15496913

DOI: 10.1038/nature03001

PubMed ID: 15815621

Title: Generation and annotation of the DNA sequences of human chromosomes 2 and 4.

PubMed ID: 15815621

DOI: 10.1038/nature03466

PubMed ID: 16964243

Title: A probability-based approach for high-throughput protein phosphorylation analysis and site localization.

PubMed ID: 16964243

DOI: 10.1038/nbt1240

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 19413330

Title: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.

PubMed ID: 19413330

DOI: 10.1021/ac9004309

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

Sequence Information:

  • Length: 539
  • Mass: 58499
  • Checksum: 6A2F0080991B2271
  • Sequence:
    • MPGQRRALSP KMASMRESDT GLWLHNKLGA TDELWAPPSI ASLLTAAVID NIRLCFHGLS 
SAVKLKLLLG TLHLPRRTVD EMKGALMEII QLASLDSDPW VLMVADILKS FPDTGSLNLE 
LEEQNPNVQD ILGELREKVG ECEASAMLPL ECQYLNKNAL TTLAGPLTPP VKHFQLKRKP 
KSATLRAELL QKSTETAQQL KRSAGVPFHA KGRGLLRKMD TTTPLKGIPK QAPFRSPTAP 
SVFSPTGNRT PIPPSRTLLR KERGVKLLDI SELDMVGAGR EAKRRRKTLD AEVVEKPAKE 
ETVVENATPD YAAGLVSTQK LGSLNNEPAL PSTSYLPSTP SVVPASSYIP SSETPPAPSS 
REASRPPEEP SAPSPTLPAQ FKQRAPMYNS GLSPATPTPA APTSPLTPTT PPAVAPTTQT 
PPVAMVAPQT QAPAQQQPKK NLSLTREQMF AAQEMFKTAN KVTRPEKALI LGFMAGSREN 
PCQEQGDVIQ IKLSEHTEDL PKADGQGSTT MLVDTVFEMN YATGQWTRFK KYKPMTNVS