Details for: CHAF1B

Gene ID: 8208

Symbol: CHAF1B

Ensembl ID: ENSG00000159259

Description: chromatin assembly factor 1 subunit B

Associated with

Cells (max top 100)

(Cell Significance Index and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: polychromatophilic erythroblast (CL0000550)
    Fold Change: 30.7949
    Cell Significance Index: -4.7900
  • Cell Name: hematopoietic oligopotent progenitor cell (CL0002032)
    Fold Change: 21.3292
    Cell Significance Index: -5.4100
  • Cell Name: orthochromatic erythroblast (CL0000552)
    Fold Change: 5.2232
    Cell Significance Index: -6.4400
  • Cell Name: CD8-alpha-beta-positive, alpha-beta intraepithelial T cell (CL0000796)
    Fold Change: 2.2622
    Cell Significance Index: -6.0600
  • Cell Name: stromal cell of bone marrow (CL0010001)
    Fold Change: 1.7207
    Cell Significance Index: -6.7900
  • Cell Name: epidermal Langerhans cell (CL0002457)
    Fold Change: 1.1971
    Cell Significance Index: -2.6200
  • Cell Name: colon goblet cell (CL0009039)
    Fold Change: 0.8507
    Cell Significance Index: 84.1600
  • Cell Name: gut absorptive cell (CL0000677)
    Fold Change: 0.5865
    Cell Significance Index: 35.2100
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 0.5000
    Cell Significance Index: 54.3900
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.4876
    Cell Significance Index: 79.3100
  • Cell Name: pro-T cell (CL0000827)
    Fold Change: 0.3511
    Cell Significance Index: 8.9700
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: 0.2816
    Cell Significance Index: 7.8700
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 0.2569
    Cell Significance Index: 51.5300
  • Cell Name: enteroendocrine cell of colon (CL0009042)
    Fold Change: 0.2477
    Cell Significance Index: 47.1400
  • Cell Name: neutrophil progenitor cell (CL0000834)
    Fold Change: 0.2150
    Cell Significance Index: 5.7500
  • Cell Name: epithelial cell of stomach (CL0002178)
    Fold Change: 0.2129
    Cell Significance Index: 24.8100
  • Cell Name: type II muscle cell (CL0002212)
    Fold Change: 0.2121
    Cell Significance Index: 3.4200
  • Cell Name: retinal progenitor cell (CL0002672)
    Fold Change: 0.2081
    Cell Significance Index: 11.6800
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.1966
    Cell Significance Index: 39.0100
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 0.1914
    Cell Significance Index: 22.5700
  • Cell Name: mesonephric nephron tubule epithelial cell (CL1000022)
    Fold Change: 0.1761
    Cell Significance Index: 6.1200
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 0.1405
    Cell Significance Index: 126.8300
  • Cell Name: GABAergic interneuron (CL0011005)
    Fold Change: 0.1284
    Cell Significance Index: 88.7900
  • Cell Name: conjunctival epithelial cell (CL1000432)
    Fold Change: 0.1239
    Cell Significance Index: 1.6900
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: 0.1236
    Cell Significance Index: 3.9600
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: 0.1222
    Cell Significance Index: 7.5000
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: 0.1174
    Cell Significance Index: 6.1000
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 0.1119
    Cell Significance Index: 61.1100
  • Cell Name: intermediate cell of urothelium (CL4030055)
    Fold Change: 0.0879
    Cell Significance Index: 15.8400
  • Cell Name: hair follicular keratinocyte (CL2000092)
    Fold Change: 0.0818
    Cell Significance Index: 36.1800
  • Cell Name: intestinal crypt stem cell of small intestine (CL0009017)
    Fold Change: 0.0756
    Cell Significance Index: 1.6100
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: 0.0714
    Cell Significance Index: 1.2000
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.0666
    Cell Significance Index: 1.9200
  • Cell Name: basal cell of urothelium (CL1000486)
    Fold Change: 0.0613
    Cell Significance Index: 7.5400
  • Cell Name: obsolete caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: 0.0520
    Cell Significance Index: 18.6500
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 0.0465
    Cell Significance Index: 3.2200
  • Cell Name: basal cell of prostate epithelium (CL0002341)
    Fold Change: 0.0434
    Cell Significance Index: 1.1800
  • Cell Name: paneth cell of epithelium of small intestine (CL1000343)
    Fold Change: 0.0351
    Cell Significance Index: 0.7600
  • Cell Name: pancreatic ductal cell (CL0002079)
    Fold Change: 0.0291
    Cell Significance Index: 3.3400
  • Cell Name: acinar cell of salivary gland (CL0002623)
    Fold Change: 0.0281
    Cell Significance Index: 1.3100
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: 0.0251
    Cell Significance Index: 1.6200
  • Cell Name: enterocyte of epithelium of large intestine (CL0002071)
    Fold Change: 0.0210
    Cell Significance Index: 0.9500
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: 0.0179
    Cell Significance Index: 33.7300
  • Cell Name: eye photoreceptor cell (CL0000287)
    Fold Change: 0.0117
    Cell Significance Index: 0.7400
  • Cell Name: enteroendocrine cell of small intestine (CL0009006)
    Fold Change: 0.0116
    Cell Significance Index: 0.2900
  • Cell Name: fibroblast of dermis (CL0002551)
    Fold Change: 0.0105
    Cell Significance Index: 0.2200
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.0065
    Cell Significance Index: 0.2300
  • Cell Name: stromal cell of ovary (CL0002132)
    Fold Change: 0.0046
    Cell Significance Index: 0.6300
  • Cell Name: lactocyte (CL0002325)
    Fold Change: 0.0035
    Cell Significance Index: 0.4500
  • Cell Name: non-pigmented ciliary epithelial cell (CL0002304)
    Fold Change: 0.0015
    Cell Significance Index: 0.9700
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: 0.0002
    Cell Significance Index: 0.0400
  • Cell Name: lens epithelial cell (CL0002224)
    Fold Change: -0.0016
    Cell Significance Index: -2.4900
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: -0.0017
    Cell Significance Index: -3.0700
  • Cell Name: odontoblast (CL0000060)
    Fold Change: -0.0028
    Cell Significance Index: -0.3600
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: -0.0041
    Cell Significance Index: -0.1100
  • Cell Name: pigmented ciliary epithelial cell (CL0002303)
    Fold Change: -0.0046
    Cell Significance Index: -0.6700
  • Cell Name: secondary lens fiber (CL0002225)
    Fold Change: -0.0050
    Cell Significance Index: -6.7400
  • Cell Name: pulmonary alveolar epithelial cell (CL0000322)
    Fold Change: -0.0069
    Cell Significance Index: -5.2100
  • Cell Name: cerebellar granule cell (CL0001031)
    Fold Change: -0.0076
    Cell Significance Index: -0.1300
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: -0.0077
    Cell Significance Index: -3.4900
  • Cell Name: kidney loop of Henle cortical thick ascending limb epithelial cell (CL1001109)
    Fold Change: -0.0078
    Cell Significance Index: -5.7300
  • Cell Name: sebum secreting cell (CL0000317)
    Fold Change: -0.0085
    Cell Significance Index: -0.6000
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: -0.0087
    Cell Significance Index: -6.4300
  • Cell Name: pancreatic PP cell (CL0002275)
    Fold Change: -0.0114
    Cell Significance Index: -7.1100
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: -0.0117
    Cell Significance Index: -6.6000
  • Cell Name: indirect pathway medium spiny neuron (CL4023029)
    Fold Change: -0.0136
    Cell Significance Index: -0.6000
  • Cell Name: direct pathway medium spiny neuron (CL4023026)
    Fold Change: -0.0137
    Cell Significance Index: -0.5200
  • Cell Name: luminal adaptive secretory precursor cell of mammary gland (CL4033057)
    Fold Change: -0.0138
    Cell Significance Index: -0.6500
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: -0.0168
    Cell Significance Index: -4.8400
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: -0.0264
    Cell Significance Index: -2.7000
  • Cell Name: CD4-positive, alpha-beta memory T cell, CD45RO-positive (CL0001204)
    Fold Change: -0.0266
    Cell Significance Index: -0.7800
  • Cell Name: hippocampal granule cell (CL0001033)
    Fold Change: -0.0284
    Cell Significance Index: -1.9100
  • Cell Name: skeletal muscle myoblast (CL0000515)
    Fold Change: -0.0290
    Cell Significance Index: -0.3200
  • Cell Name: progenitor cell of mammary luminal epithelium (CL0009116)
    Fold Change: -0.0295
    Cell Significance Index: -2.2000
  • Cell Name: pancreatic D cell (CL0000173)
    Fold Change: -0.0353
    Cell Significance Index: -7.4400
  • Cell Name: Purkinje cell (CL0000121)
    Fold Change: -0.0420
    Cell Significance Index: -0.9200
  • Cell Name: glycinergic neuron (CL1001509)
    Fold Change: -0.0432
    Cell Significance Index: -2.2700
  • Cell Name: smooth muscle cell of sphincter of pupil (CL0002243)
    Fold Change: -0.0572
    Cell Significance Index: -5.9600
  • Cell Name: cortical cell of adrenal gland (CL0002097)
    Fold Change: -0.0601
    Cell Significance Index: -1.6100
  • Cell Name: forebrain neuroblast (CL1000042)
    Fold Change: -0.0618
    Cell Significance Index: -3.8000
  • Cell Name: respiratory goblet cell (CL0002370)
    Fold Change: -0.0620
    Cell Significance Index: -0.6400
  • Cell Name: lung endothelial cell (CL1001567)
    Fold Change: -0.0657
    Cell Significance Index: -3.4200
  • Cell Name: medial ganglionic eminence derived interneuron (CL4023063)
    Fold Change: -0.0712
    Cell Significance Index: -1.0200
  • Cell Name: type I muscle cell (CL0002211)
    Fold Change: -0.0719
    Cell Significance Index: -1.7600
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: -0.0736
    Cell Significance Index: -5.6500
  • Cell Name: kidney loop of Henle descending limb epithelial cell (CL1001021)
    Fold Change: -0.0790
    Cell Significance Index: -6.2600
  • Cell Name: skeletal muscle fiber (CL0008002)
    Fold Change: -0.0821
    Cell Significance Index: -2.1100
  • Cell Name: basal cell of epidermis (CL0002187)
    Fold Change: -0.0948
    Cell Significance Index: -1.4400
  • Cell Name: intestinal epithelial cell (CL0002563)
    Fold Change: -0.1029
    Cell Significance Index: -1.0700
  • Cell Name: epithelial cell of uterus (CL0002149)
    Fold Change: -0.1074
    Cell Significance Index: -1.4900
  • Cell Name: granulosa cell (CL0000501)
    Fold Change: -0.1099
    Cell Significance Index: -2.8900
  • Cell Name: transit amplifying cell of small intestine (CL0009012)
    Fold Change: -0.1118
    Cell Significance Index: -2.3200
  • Cell Name: hippocampal pyramidal neuron (CL1001571)
    Fold Change: -0.1202
    Cell Significance Index: -3.4300
  • Cell Name: corticothalamic-projecting glutamatergic cortical neuron (CL4023013)
    Fold Change: -0.1209
    Cell Significance Index: -3.8500
  • Cell Name: BEST4+ enteroycte (CL4030026)
    Fold Change: -0.1238
    Cell Significance Index: -1.8700
  • Cell Name: L6b glutamatergic cortical neuron (CL4023038)
    Fold Change: -0.1283
    Cell Significance Index: -4.2000
  • Cell Name: L5 extratelencephalic projecting glutamatergic cortical neuron (CL4023041)
    Fold Change: -0.1285
    Cell Significance Index: -4.5000
  • Cell Name: pvalb GABAergic cortical interneuron (CL4023018)
    Fold Change: -0.1291
    Cell Significance Index: -2.7400
  • Cell Name: germ cell (CL0000586)
    Fold Change: -0.1417
    Cell Significance Index: -1.0700
  • Cell Name: thymocyte (CL0000893)
    Fold Change: -0.1559
    Cell Significance Index: -1.9700

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics:** CHAF1B is a 60-kDa protein that belongs to the Caf-1 complex, a protein complex involved in chromatin assembly and DNA replication. It is a subunit of the Caf-1 complex, which is composed of three subunits: CAF1A, CAF1B, and CAF1C. CHAF1B is characterized by its ability to bind histones and facilitate nucleosome assembly, which is essential for chromatin formation and DNA replication. Its high expression levels in various cell types, including germ cells, cardiac endothelial cells, and intestinal epithelial cells, indicate its importance in maintaining cellular homeostasis. **Pathways and Functions:** CHAF1B is involved in several key pathways, including: 1. **Chromatin Assembly:** CHAF1B plays a crucial role in chromatin assembly by facilitating histone binding and nucleosome assembly. This process is essential for maintaining chromatin structure and regulating gene expression. 2. **DNA Replication:** CHAF1B is involved in DNA replication-dependent chromatin assembly, which is critical for cell cycle progression and DNA repair. 3. **Cell Cycle:** CHAF1B is expressed in various cell types, including germ cells, cardiac endothelial cells, and intestinal epithelial cells, which suggests its involvement in cell cycle regulation. 4. **DNA Repair:** CHAF1B is also involved in DNA repair pathways, which is essential for maintaining genome stability and preventing disease. **Clinical Significance:** Dysregulation of CHAF1B has been implicated in various human diseases, including: 1. **Cancer:** Alterations in CHAF1B expression have been observed in various types of cancer, including breast, lung, and colon cancer. This suggests that CHAF1B may play a role in tumorigenesis and cancer progression. 2. **Neurodegenerative Diseases:** CHAF1B has been implicated in neurodegenerative diseases, including Alzheimer's disease and Parkinson's disease. Its dysregulation may contribute to disease pathology and progression. 3. **Cardiovascular Diseases:** CHAF1B has been linked to cardiovascular diseases, including atherosclerosis and cardiac hypertrophy. Its dysregulation may contribute to disease development and progression. In conclusion, CHAF1B is a crucial protein component of the Caf-1 complex, which plays a pivotal role in chromatin assembly and DNA replication. Its widespread expression in various cell types and involvement in key pathways, including chromatin assembly, DNA replication, cell cycle, and DNA repair, underscores its importance in maintaining cellular homeostasis. Dysregulation of CHAF1B has been implicated in various human diseases, highlighting the need for further research into its role in disease pathology and potential therapeutic applications.

Genular Protein ID: 3300473273

Symbol: CAF1B_HUMAN

Name: Chromatin assembly factor I p60 subunit

UniProtKB Accession Codes:

Q13112 Q99548

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CORUM 1 CTD 1 ChiTaRS 1 ComplexPortal 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 5 EMDB 8 Ensembl 1 GO 13 Gene3D 1 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 7 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 MetOSite 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 11 PDBsum 11 PIR 1 PRINTS 1 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 RefSeq 3 SIGNOR 1 SMART 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 7600578

Title: The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication.

PubMed ID: 7600578

DOI: 10.1016/s0092-8674(05)80015-7

PubMed ID: 10830953

Title: The DNA sequence of human chromosome 21.

PubMed ID: 10830953

DOI: 10.1038/35012518

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 8885239

Title: Identification of novel M phase phosphoproteins by expression cloning.

PubMed ID: 8885239

DOI: 10.1091/mbc.7.9.1455

PubMed ID: 9813080

Title: Recruitment of phosphorylated chromatin assembly factor 1 to chromatin after UV irradiation of human cells.

PubMed ID: 9813080

DOI: 10.1083/jcb.143.3.563

PubMed ID: 9614144

Title: Nucleosome assembly activity and intracellular localization of human CAF-1 changes during the cell division cycle.

PubMed ID: 9614144

DOI: 10.1074/jbc.273.24.15279

PubMed ID: 10893180

Title: CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair.

PubMed ID: 10893180

DOI: 10.1242/jcs.113.15.2647

PubMed ID: 17081983

Title: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.

PubMed ID: 17081983

DOI: 10.1016/j.cell.2006.09.026

PubMed ID: 16964243

Title: A probability-based approach for high-throughput protein phosphorylation analysis and site localization.

PubMed ID: 16964243

DOI: 10.1038/nbt1240

PubMed ID: 18220336

Title: Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.

PubMed ID: 18220336

DOI: 10.1021/pr0705441

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 19413330

Title: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.

PubMed ID: 19413330

DOI: 10.1021/ac9004309

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 19608861

Title: Lysine acetylation targets protein complexes and co-regulates major cellular functions.

PubMed ID: 19608861

DOI: 10.1126/science.1175371

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 21406692

Title: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.

PubMed ID: 21406692

DOI: 10.1126/scisignal.2001570

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 33857403

Title: DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network.

PubMed ID: 33857403

DOI: 10.1016/j.molcel.2021.03.041

Sequence Information:

  • Length: 559
  • Mass: 61493
  • Checksum: AD1846CC81B8DC9F
  • Sequence:
    • MKVITCEIAW HNKEPVYSLD FQHGTAGRIH RLASAGVDTN VRIWKVEKGP DGKAIVEFLS 
NLARHTKAVN VVRFSPTGEI LASGGDDAVI LLWKVNDNKE PEQIAFQDED EAQLNKENWT 
VVKTLRGHLE DVYDICWATD GNLMASASVD NTAIIWDVSK GQKISIFNEH KSYVQGVTWD 
PLGQYVATLS CDRVLRVYSI QKKRVAFNVS KMLSGIGAEG EARSYRMFHD DSMKSFFRRL 
SFTPDGSLLL TPAGCVESGE NVMNTTYVFS RKNLKRPIAH LPCPGKATLA VRCCPVYFEL 
RPVVETGVEL MSLPYRLVFA VASEDSVLLY DTQQSFPFGY VSNIHYHTLS DISWSSDGAF 
LAISSTDGYC SFVTFEKDEL GIPLKEKPVL NMRTPDTAKK TKSQTHRGSS PGPRPVEGTP 
ASRTQDPSSP GTTPPQARQA PAPTVIRDPP SITPAVKSPL PGPSEEKTLQ PSSQNTKAHP 
SRRVTLNTLQ AWSKTTPRRI NLTPLKTDTP PSSVPTSVIS TPSTEEIQSE TPGDAQGSPP 
ELKRPRLDEN KGGTESLDP

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.