Details for: UPF1

Gene ID: 5976

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: UPF1

Ensembl ID: ENSG00000005007

Description: UPF1 RNA helicase and ATPase

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • nasal mucosa goblet cell CL0002480
    CSI 7.51
    rCSI 8.71%
    PRS 79.45
  • renal principal cell CL0005009
    CSI 6.35
    rCSI 16.49%
    PRS 75.96
  • astrocyte of the cerebral cortex CL0002605
    CSI 5.45
    rCSI 12.21%
    PRS 56.36
  • intestinal tuft cell CL0019032
    CSI 4.97
    rCSI 7.59%
    PRS 78.48
  • retinal blood vessel endothelial cell CL0002585
    CSI 4.68
    rCSI 7.48%
    PRS 78.02
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 3.77
    rCSI 6.66%
    PRS 54.83
  • ciliated cell CL0000064
    CSI 3.72
    rCSI 6.03%
    PRS 69.81
  • secretory cell CL0000151
    CSI 3.42
    rCSI 3.57%
    PRS 74.03
  • group 3 innate lymphoid cell CL0001071
    CSI 3.23
    rCSI 2.43%
    PRS 80.4
  • Kupffer cell CL0000091
    CSI 3.05
    rCSI 6.97%
    PRS 75.03
  • pro-B cell CL0000826
    CSI 2.99
    rCSI 2.48%
    PRS 76.83
  • CD4-positive, alpha-beta memory T cell CL0000897
    CSI 2.92
    rCSI 2.1%
    PRS 87.45
  • VIP GABAergic cortical interneuron CL4023016
    CSI 2.92
    rCSI 3.48%
    PRS 55.67
  • CD4-positive helper T cell CL0000492
    CSI 2.91
    rCSI 2.2%
    PRS 86.98
  • dendritic cell, human CL0001056
    CSI 2.85
    rCSI 4.37%
    PRS 83.41
  • midzonal region hepatocyte CL0019028
    CSI 2.81
    rCSI 6.59%
    PRS 75.52
  • CD14-low, CD16-positive monocyte CL0002396
    CSI 2.78
    rCSI 2.14%
    PRS 76.63
  • neural crest cell CL0011012
    CSI 2.78
    rCSI 2.2%
    PRS 61.95
  • duct epithelial cell CL0000068
    CSI 2.62
    rCSI 3.83%
    PRS 79.28
  • multi-ciliated epithelial cell CL0005012
    CSI 2.6
    rCSI 2.59%
    PRS 67.95
  • fibroblast of lung CL0002553
    CSI 2.48
    rCSI 2.31%
    PRS 74.97
  • mucus secreting cell CL0000319
    CSI 2.48
    rCSI 3.93%
    PRS 83.6
  • bronchus fibroblast of lung CL2000093
    CSI 2.35
    rCSI 1.91%
    PRS 74.08
  • stem cell CL0000034
    CSI 2.3
    rCSI 2.22%
    PRS 66.58
  • hepatocyte CL0000182
    CSI 2.29
    rCSI 4.11%
    PRS 73.6
  • erythroblast CL0000765
    CSI 2.21
    rCSI 5.88%
    PRS 81.5
  • chondrocyte CL0000138
    CSI 2.18
    rCSI 3.46%
    PRS 66.9
  • goblet cell CL0000160
    CSI 2.17
    rCSI 2.05%
    PRS 73.52
  • lung secretory cell CL1000272
    CSI 2.15
    rCSI 5.32%
    PRS 73.4
  • ciliated epithelial cell CL0000067
    CSI 2.14
    rCSI 1.88%
    PRS 62.77
  • club cell CL0000158
    CSI 2.09
    rCSI 3.06%
    PRS 69.01
  • ionocyte CL0005006
    CSI 2.07
    rCSI 2.22%
    PRS 75.03
  • ependymal cell CL0000065
    CSI 2.07
    rCSI 4.2%
    PRS 52.37
  • kidney interstitial alternatively activated macrophage CL1000695
    CSI 2.07
    rCSI 5.39%
    PRS 74.79
  • epithelial cell of lung CL0000082
    CSI 2.05
    rCSI 1.7%
    PRS 74.7
  • foveolar cell of stomach CL0002179
    CSI 2.04
    rCSI 4.35%
    PRS 81.88
  • lung ciliated cell CL1000271
    CSI 2.04
    rCSI 2.36%
    PRS 65.9
  • conjunctival epithelial cell CL1000432
    CSI 2.02
    rCSI 3.09%
    PRS 74.59
  • perivascular cell CL4033054
    CSI 2.02
    rCSI 2.77%
    PRS 79.21
  • vascular leptomeningeal cell CL4023051
    CSI 1.98
    rCSI 3.48%
    PRS 67.15
  • cerebral cortex endothelial cell CL1001602
    CSI 1.95
    rCSI 3.38%
    PRS 65.12
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 1.87
    rCSI 1.69%
    PRS 71.94
  • basal cell CL0000646
    CSI 1.87
    rCSI 2.5%
    PRS 73.34
  • myeloid leukocyte CL0000766
    CSI 1.85
    rCSI 1.71%
    PRS 75.92
  • adventitial cell CL0002503
    CSI 1.82
    rCSI 4.36%
    PRS 79.44
  • promyelocyte CL0000836
    CSI 1.79
    rCSI 2.58%
    PRS 81.43
  • hepatic stellate cell CL0000632
    CSI 1.79
    rCSI 6.7%
    PRS 66.34
  • brush cell CL0002204
    CSI 1.78
    rCSI 3.52%
    PRS 85.38
  • transit amplifying cell of colon CL0009011
    CSI 1.73
    rCSI 2.04%
    PRS 76.41
  • intermediate monocyte CL0002393
    CSI 1.65
    rCSI 2.5%
    PRS 79.54
  • squamous epithelial cell CL0000076
    CSI 1.64
    rCSI 3.88%
    PRS 75.87
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 1.63
    rCSI 2.03%
    PRS 53.7
  • extravillous trophoblast CL0008036
    CSI 1.61
    rCSI 2%
    PRS 71.75
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 1.58
    rCSI 2.66%
    PRS 55.71
  • BEST4+ enteroycte CL4030026
    CSI 1.57
    rCSI 1.95%
    PRS 75.68
  • colon epithelial cell CL0011108
    CSI 1.56
    rCSI 1.63%
    PRS 71.44
  • cerebral cortex neuron CL0010012
    CSI 1.51
    rCSI 6.15%
    PRS 66.68
  • mononuclear phagocyte CL0000113
    CSI 1.5
    rCSI 3.3%
    PRS 78.41
  • intestine goblet cell CL0019031
    CSI 1.48
    rCSI 1.31%
    PRS 72.24
  • fallopian tube secretory epithelial cell CL4030006
    CSI 1.48
    rCSI 1.42%
    PRS 73.62
  • myoepithelial cell CL0000185
    CSI 1.45
    rCSI 3.68%
    PRS 80.17
  • placental villous trophoblast CL2000060
    CSI 1.45
    rCSI 2.24%
    PRS 73.29
  • ciliated columnar cell of tracheobronchial tree CL0002145
    CSI 1.41
    rCSI 3.22%
    PRS 68.86
  • sst GABAergic cortical interneuron CL4023017
    CSI 1.41
    rCSI 1.82%
    PRS 57.01
  • kidney connecting tubule epithelial cell CL1000768
    CSI 1.38
    rCSI 3.49%
    PRS 64.23
  • innate lymphoid cell CL0001065
    CSI 1.36
    rCSI 2.81%
    PRS 72.76
  • pancreatic acinar cell CL0002064
    CSI 1.22
    rCSI 1.62%
    PRS 80.31
  • tracheal goblet cell CL1000329
    CSI 1.17
    rCSI 2.56%
    PRS 83.13
  • intestinal crypt stem cell of small intestine CL0009017
    CSI 1.15
    rCSI 3.11%
    PRS 80.07
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 1.14
    rCSI 2.76%
    PRS 53.87
  • type B pancreatic cell CL0000169
    CSI 1.04
    rCSI 2.31%
    PRS 73.2
  • deuterosomal cell CL4033044
    CSI 0.8
    rCSI 2.69%
    PRS 74.12
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 0.78
    rCSI 2.93%
    PRS 56.17
  • respiratory goblet cell CL0002370
    CSI 0.64
    rCSI 6.97%
    PRS 84.49
  • chandelier pvalb GABAergic cortical interneuron CL4023036
    CSI 0.63
    rCSI 1.98%
    PRS 59.95
  • bronchial goblet cell CL1000312
    CSI 0.62
    rCSI 2.49%
    PRS 84.65
  • L6b glutamatergic cortical neuron CL4023038
    CSI 0.53
    rCSI 1.66%
    PRS 57.52
  • eosinophil CL0000771
    CSI 0.4
    rCSI 2.6%
    PRS 92.62
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 0.32
    rCSI 1.16%
    PRS 53.8

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

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  • Node Color (Target Cell CSI, relative to current network):
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    • High
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  • Node Size: Proportional to Target Cell CSI magnitude
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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [UPF1](/details-gene/5976) (Up-frameshift suppressor 1 homolog) is a highly conserved protein-coding gene located on chromosome 19p13.11. It encodes a key RNA helicase and ATPase that serves as the central regulator of the nonsense-mediated mRNA decay (NMD) pathway, a critical cellular surveillance mechanism that identifies and degrades mRNAs containing premature termination codons. Its fundamental role in maintaining transcriptomic integrity is underscored by its broad expression across diverse and highly specialized cell types, including secretory cells like the [nasal mucosa goblet cell](/details-cell/CL0002480) and neural cells such as the [astrocyte of the cerebral cortex](/details-cell/CL0002605). Beyond NMD, [UPF1](/details-gene/5976) is also implicated in other crucial cellular processes, including DNA repair and telomere maintenance, highlighting its multifaceted role in preserving cellular homeostasis. Dysregulation of [UPF1](/details-gene/5976) is associated with human disease, as indicated by its OMIM entry ([601430](https://omim.org/entry/601430)). ## Cellular Roles and Expression Landscape The expression profile of [UPF1](/details-gene/5976) suggests it is a ubiquitously expressed and functionally indispensable gene, rather than a marker for a specific cell lineage. Its significance is particularly high in cells characterized by high metabolic activity, complex morphology, or specialized secretory functions. **Overall**, the gene shows the highest significance (CSI: 7.51) in the [nasal mucosa goblet cell](/details-cell/CL0002480), a highly active secretory cell type. This is followed by other functionally specialized cells such as the [renal principal cell](/details-cell/CL0005009) (CSI: 6.35) and [intestinal tuft cell](/details-cell/CL0019032) (CSI: 4.97). This pattern suggests that cells with high transcriptional and translational loads may be particularly dependent on the RNA quality control functions of [UPF1](/details-gene/5976) to prevent the accumulation of aberrant proteins. Furthermore, [UPF1](/details-gene/5976) is highly significant in various neural cell types, including the [astrocyte of the cerebral cortex](/details-cell/CL0002605) (CSI: 5.45) and multiple cortical interneurons. This is consistent with the need for stringent quality control of the complex and long-lived transcripts required for proper neuronal function. The gene also plays a notable role within the immune system, showing significant expression in both innate immune cells like the [Kupffer cell](/details-cell/CL0000091) and [group 3 innate lymphoid cell](/details-cell/CL0001071), as well as in adaptive immune cells such as the [pro-B cell](/details-cell/CL0000826) and [CD4-positive helper T cell](/details-cell/CL0000492). This broad expression across functionally diverse cell types highlights its role as a fundamental component of cellular machinery. ## Pathways and Molecular Function The primary and most well-characterized function of [UPF1](/details-gene/5976) is its central role in RNA surveillance. Functional annotations confirm its involvement in the [nuclear-transcribed mRNA catabolic process, nonsense-mediated decay](/details-ontology/GO:0000184) ([Link](https://doi.org/10.1073/pnas.93.20.10928), [Link](https://doi.org/10.1093/nar/25.4.814)). This process is detailed in Reactome pathways, including [Nonsense-mediated decay (NMD)](/details-pathway/R-HSA-927802) and its variants dependent on the [exon junction complex (EJC)](/details-pathway/R-HSA-975957). The molecular basis for this function is its intrinsic [RNA helicase activity](/details-ontology/GO:0003724) and [ATP hydrolysis activity](/details-ontology/GO:0016887), which are essential for remodeling mRNA-protein complexes during the surveillance process ([Link](https://doi.org/10.1017/s1355838200000546)). [UPF1](/details-gene/5976) is a core component of the surveillance complex and is known to physically associate with the [exon-exon junction complex](/details-ontology/GO:0035145), a key signal for NMD activation ([Link](https://doi.org/10.1126/science.1062786)). Its function is tightly regulated by phosphorylation, which mediates its interaction with other factors like SMG-5 and SMG-7 ([Link](https://doi.org/10.1016/s1097-2765(03)00443-x)). The protein localizes to multiple subcellular compartments, including the [cytoplasm](/details-ontology/GO:0005737), [nucleus](/details-ontology/GO:0005634), and specialized RNA processing sites like [P-bodies](/details-ontology/GO:0000932), reflecting its dynamic role in mRNA metabolism. Beyond RNA decay, annotations suggest a broader role for [UPF1](/details-gene/5976) in maintaining genome integrity, with functions in [DNA repair](/details-ontology/GO:0006281), [DNA replication](/details-ontology/GO:0006260), and [regulation of telomere maintenance](/details-ontology/GO:0032204). This is supported by its ability to bind [chromatin](/details-ontology/GO:0000785) and its annotated [DNA duplex unwinding](/details-ontology/GO:0032508) activity, suggesting it may act at the interface of transcription, DNA repair, and RNA quality control. ## Research Directions The ubiquitous and critical nature of [UPF1](/details-gene/5976) makes it a central node in cellular homeostasis. Its high expression in specialized, long-lived, or highly active cells provides a foundation for investigating its role in cell-type-specific vulnerabilities. **Testable Hypotheses:** 1. The high significance of [UPF1](/details-gene/5976) in terminally differentiated, non-dividing cells like neurons and highly active secretory cells like goblet cells suggests these cell types are exceptionally vulnerable to proteotoxic stress caused by aberrant transcripts. Therefore, age-related decline in [UPF1](/details-gene/5976) function could be a key contributor to cellular dysfunction in neurodegenerative diseases and pathologies of mucosal surfaces. 2. Given its dual roles in [DNA repair](/details-ontology/GO:0006281) and RNA surveillance, [UPF1](/details-gene/5976) may function as a master regulator that coordinates the cellular response to genotoxic stress. It may be recruited to sites of DNA damage to locally suppress transcription or modulate the stability of mRNAs encoding DNA repair factors, thus preventing the translation of damaged templates while promoting repair. **Proposed Experiment:** To test the first hypothesis regarding the heightened dependency of secretory cells on [UPF1](/details-gene/5976), a conditional knockout mouse model could be employed. Crossing a *Upf1-floxed* mouse with a line expressing Cre recombinase under a goblet cell-specific promoter (e.g., *Muc2-CreERT2*) would allow for inducible deletion of [UPF1](/details-gene/5976) specifically in intestinal goblet cells. Following tamoxifen induction, intestinal tissues could be analyzed by RNA-sequencing to identify transcripts that escape NMD, proteomics to detect truncated protein products, and immunohistochemistry to assess cellular integrity, mucus production, and activation of the unfolded protein response (UPR). This experiment would directly test whether loss of [UPF1](/details-gene/5976) function compromises the viability and specialized function of these secretory cells. **Therapeutic Potential:** As a fundamental housekeeping gene, global inhibition of [UPF1](/details-gene/5976) would likely be highly toxic. However, modulation of its activity presents a compelling therapeutic strategy. For genetic diseases caused by nonsense mutations (e.g., in cystic fibrosis or Duchenne muscular dystrophy), controlled, partial inhibition of [UPF1](/details-gene/5976) could promote translational readthrough of premature stop codons, restoring partial function of the truncated protein. Conversely, in certain cancers that rely on mutated transcripts or viral oncogenes sensitive to NMD, enhancing [UPF1](/details-gene/5976) activity could be a novel anti-cancer strategy. Therefore, developing small molecules that can fine-tune, rather than ablate, [UPF1](/details-gene/5976) function holds significant therapeutic promise.

Genular Protein ID: 3508377753

Symbol: RENT1_HUMAN

Name: Up-frameshift suppressor 1 homolog

UniProtKB Accession Codes:

Q92900 O00239 O43343 Q86Z25 Q92842

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 BRENDA 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 2 CDD 4 CORUM 1 CTD 1 ChEBI 27 ChiTaRS 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 EC 2 EMBL 6 EMDB 7 Ensembl 2 EvolutionaryTrace 1 ExpressionAtlas 1 GO 36 Gene3D 3 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyCosmos 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 IDEAL 1 InParanoid 1 IntAct 1 InterPro 8 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MalaCards 1 MassIVE 1 MetOSite 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 11 PDBsum 11 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 5 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 2 Pumba 1 RNAct 1 Reactome 2 RefSeq 2 Rhea 4 SIGNOR 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 8855285

Title: Mammalian orthologues of a yeast regulator of nonsense transcript stability.

PubMed ID: 8855285

DOI: 10.1073/pnas.93.20.10928

PubMed ID: 9064659

Title: Cloning and characterization of HUPF1, a human homolog of the Saccharomyces cerevisiae nonsense mRNA-reducing UPF1 protein.

PubMed ID: 9064659

DOI: 10.1093/nar/25.4.814

PubMed ID: 9039502

Title: Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain.

PubMed ID: 9039502

DOI: 10.1093/dnares/3.5.321

PubMed ID: 10454541

Title: SMG-2 is a phosphorylated protein required for mRNA surveillance in Caenorhabditis elegans and related to Upf1p of yeast.

PubMed ID: 10454541

DOI: 10.1128/mcb.19.9.5943

PubMed ID: 15057824

Title: The DNA sequence and biology of human chromosome 19.

PubMed ID: 15057824

DOI: 10.1038/nature02399

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 11163187

Title: Human Upf proteins target an mRNA for nonsense-mediated decay when bound downstream of a termination codon.

PubMed ID: 11163187

DOI: 10.1016/s0092-8674(00)00214-2

PubMed ID: 11073994

Title: Novel Upf2p orthologues suggest a functional link between translation initiation and nonsense surveillance complexes.

PubMed ID: 11073994

DOI: 10.1128/mcb.20.23.8944-8957.2000

PubMed ID: 10999600

Title: Characterization of the biochemical properties of the human Upf1 gene product that is involved in nonsense-mediated mRNA decay.

PubMed ID: 10999600

DOI: 10.1017/s1355838200000546

PubMed ID: 11544179

Title: Human SMG-1, a novel phosphatidylinositol 3-kinase-related protein kinase, associates with components of the mRNA surveillance complex and is involved in the regulation of nonsense-mediated mRNA decay.

PubMed ID: 11544179

DOI: 10.1101/gad.913001

PubMed ID: 11331269

Title: Cloning of a novel phosphatidylinositol kinase-related kinase: characterization of the human SMG-1 RNA surveillance protein.

PubMed ID: 11331269

DOI: 10.1074/jbc.c100144200

PubMed ID: 11113196

Title: Identification and characterization of human orthologues to Saccharomyces cerevisiae Upf2 protein and Upf3 protein (Caenorhabditis elegans SMG-4).

PubMed ID: 11113196

DOI: 10.1128/mcb.21.1.209-223.2001

PubMed ID: 11546874

Title: Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1.

PubMed ID: 11546874

DOI: 10.1126/science.1062786

PubMed ID: 14636577

Title: Phosphorylation of hUPF1 induces formation of mRNA surveillance complexes containing hSMG-5 and hSMG-7.

PubMed ID: 14636577

DOI: 10.1016/s1097-2765(03)00443-x

PubMed ID: 12554878

Title: Characterization of human Smg5/7a: a protein with similarities to Caenorhabditis elegans SMG5 and SMG7 that functions in the dephosphorylation of Upf1.

PubMed ID: 12554878

DOI: 10.1261/rna.2137903

PubMed ID: 15721257

Title: SMG7 is a 14-3-3-like adaptor in the nonsense-mediated mRNA decay pathway.

PubMed ID: 15721257

DOI: 10.1016/j.molcel.2005.01.010

PubMed ID: 16086026

Title: Regulated degradation of replication-dependent histone mRNAs requires both ATR and Upf1.

PubMed ID: 16086026

DOI: 10.1038/nsmb972

PubMed ID: 17081983

Title: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.

PubMed ID: 17081983

DOI: 10.1016/j.cell.2006.09.026

PubMed ID: 16452507

Title: Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay.

PubMed ID: 16452507

DOI: 10.1101/gad.1389006

PubMed ID: 17932509

Title: Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.

PubMed ID: 17932509

DOI: 10.1038/sj.embor.7401088

PubMed ID: 17525332

Title: ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.

PubMed ID: 17525332

DOI: 10.1126/science.1140321

PubMed ID: 18172165

Title: Degradation of histone mRNA requires oligouridylation followed by decapping and simultaneous degradation of the mRNA both 5' to 3' and 3' to 5'.

PubMed ID: 18172165

DOI: 10.1101/gad.1622708

PubMed ID: 18447585

Title: A competition between stimulators and antagonists of Upf complex recruitment governs human nonsense-mediated mRNA decay.

PubMed ID: 18447585

DOI: 10.1371/journal.pbio.0060111

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 18362360

Title: The editing enzyme ADAR1 and the mRNA surveillance protein hUpf1 interact in the cell nucleus.

PubMed ID: 18362360

DOI: 10.1073/pnas.0710576105

PubMed ID: 19413330

Title: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.

PubMed ID: 19413330

DOI: 10.1021/ac9004309

PubMed ID: 19417104

Title: SMG-8 and SMG-9, two novel subunits of the SMG-1 complex, regulate remodeling of the mRNA surveillance complex during nonsense-mediated mRNA decay.

PubMed ID: 19417104

DOI: 10.1101/gad.1767209

PubMed ID: 19864460

Title: Mammalian pre-mRNA 3' end processing factor CF I m 68 functions in mRNA export.

PubMed ID: 19864460

DOI: 10.1091/mbc.e09-05-0389

PubMed ID: 19150429

Title: Human proline-rich nuclear receptor coregulatory protein 2 mediates an interaction between mRNA surveillance machinery and decapping complex.

PubMed ID: 19150429

DOI: 10.1016/j.molcel.2008.11.022

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 21145460

Title: Upf1 ATPase-dependent mRNP disassembly is required for completion of nonsense- mediated mRNA decay.

PubMed ID: 21145460

DOI: 10.1016/j.cell.2010.11.043

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 21406692

Title: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.

PubMed ID: 21406692

DOI: 10.1126/scisignal.2001570

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 25220460

Title: The RNA helicase DHX34 activates NMD by promoting a transition from the surveillance to the decay-inducing complex.

PubMed ID: 25220460

DOI: 10.1016/j.celrep.2014.08.020

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 24726324

Title: MOV10 Is a 5' to 3' RNA helicase contributing to UPF1 mRNA target degradation by translocation along 3' UTRs.

PubMed ID: 24726324

DOI: 10.1016/j.molcel.2014.03.017

PubMed ID: 24129315

Title: Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.

PubMed ID: 24129315

DOI: 10.1074/mcp.o113.027870

PubMed ID: 26000482

Title: Regnase-1 and Roquin regulate a common element in inflammatory mRNAs by spatiotemporally distinct mechanisms.

PubMed ID: 26000482

DOI: 10.1016/j.cell.2015.04.029

PubMed ID: 26841701

Title: The RNA helicase DHX34 functions as a scaffold for SMG1-mediated UPF1 phosphorylation.

PubMed ID: 26841701

DOI: 10.1038/ncomms10585

PubMed ID: 27974568

Title: IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10.

PubMed ID: 27974568

DOI: 10.1128/jvi.01606-16

PubMed ID: 29382845

Title: HTLV-1 Tax plugs and freezes UPF1 helicase leading to nonsense-mediated mRNA decay inhibition.

PubMed ID: 29382845

DOI: 10.1038/s41467-017-02793-6

PubMed ID: 30218034

Title: UPF1-like helicase grip on nucleic acids dictates processivity.

PubMed ID: 30218034

DOI: 10.1038/s41467-018-06313-y

PubMed ID: 17159905

Title: Structural and functional insights into the human Upf1 helicase core.

PubMed ID: 17159905

DOI: 10.1038/sj.emboj.7601464

PubMed ID: 19556969

Title: Unusual bipartite mode of interaction between the nonsense-mediated decay factors, UPF1 and UPF2.

PubMed ID: 19556969

DOI: 10.1038/emboj.2009.175

PubMed ID: 21419344

Title: Molecular mechanisms for the RNA-dependent ATPase activity of Upf1 and its regulation by Upf2.

PubMed ID: 21419344

DOI: 10.1016/j.molcel.2011.02.010

Sequence Information:

  • Length: 1129
  • Mass: 124345
  • Checksum: 6CCA6FE42B15BA28
  • Sequence:
    • MSVEAYGPSS QTLTFLDTEE AELLGADTQG SEFEFTDFTL PSQTQTPPGG PGGPGGGGAG 
GPGGAGAGAA AGQLDAQVGP EGILQNGAVD DSVAKTSQLL AELNFEEDEE DTYYTKDLPI 
HACSYCGIHD PACVVYCNTS KKWFCNGRGN TSGSHIVNHL VRAKCKEVTL HKDGPLGETV 
LECYNCGCRN VFLLGFIPAK ADSVVVLLCR QPCASQSSLK DINWDSSQWQ PLIQDRCFLS 
WLVKIPSEQE QLRARQITAQ QINKLEELWK ENPSATLEDL EKPGVDEEPQ HVLLRYEDAY 
QYQNIFGPLV KLEADYDKKL KESQTQDNIT VRWDLGLNKK RIAYFTLPKT DSGNEDLVII 
WLRDMRLMQG DEICLRYKGD LAPLWKGIGH VIKVPDNYGD EIAIELRSSV GAPVEVTHNF 
QVDFVWKSTS FDRMQSALKT FAVDETSVSG YIYHKLLGHE VEDVIIKCQL PKRFTAQGLP 
DLNHSQVYAV KTVLQRPLSL IQGPPGTGKT VTSATIVYHL ARQGNGPVLV CAPSNIAVDQ 
LTEKIHQTGL KVVRLCAKSR EAIDSPVSFL ALHNQIRNMD SMPELQKLQQ LKDETGELSS 
ADEKRYRALK RTAERELLMN ADVICCTCVG AGDPRLAKMQ FRSILIDEST QATEPECMVP 
VVLGAKQLIL VGDHCQLGPV VMCKKAAKAG LSQSLFERLV VLGIRPIRLQ VQYRMHPALS 
AFPSNIFYEG SLQNGVTAAD RVKKGFDFQW PQPDKPMFFY VTQGQEEIAS SGTSYLNRTE 
AANVEKITTK LLKAGAKPDQ IGIITPYEGQ RSYLVQYMQF SGSLHTKLYQ EVEIASVDAF 
QGREKDFIIL SCVRANEHQG IGFLNDPRRL NVALTRARYG VIIVGNPKAL SKQPLWNHLL 
NYYKEQKVLV EGPLNNLRES LMQFSKPRKL VNTINPGARF MTTAMYDARE AIIPGSVYDR 
SSQGRPSSMY FQTHDQIGMI SAGPSHVAAM NIPIPFNLVM PPMPPPGYFG QANGPAAGRG 
TPKGKTGRGG RQKNRFGLPG PSQTNLPNSQ ASQDVASQPF SQGALTQGYI SMSQPSQMSQ 
PGLSQPELSQ DSYLGDEFKS QIDVALSQDS TYQGERAYQH GGVTGLSQY

Genular Protein ID: 1737285506

Symbol: A0A024R7L5_HUMAN

Name: N/A

UniProtKB Accession Codes:

A0A024R7L5

Database IDs:

ARBA 6 AlphaFoldDB 1 Antibodypedia 1 BioGRID-ORCS 1 CDD 4 CTD 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 4 ExpressionAtlas 1 GO 10 Gene3D 3 HOGENOM 1 InterPro 8 NCBI Taxonomy 1 OMA 1 OrthoDB 1 PANTHER 2 PROSITE 2 PROSITE-ProRule 1 Pfam 5 RefSeq 1 SAM 1 SMR 1 SUPFAM 1 VEuPathDB 1

Citations:

PubMed ID: 11181995

Title: The sequence of the human genome.

PubMed ID: 11181995

DOI: 10.1126/science.1058040

Sequence Information:

  • Length: 1118
  • Mass: 123036
  • Checksum: A485D3ED52C39D06
  • Sequence:
    • MSVEAYGPSS QTLTFLDTEE AELLGADTQG SEFEFTDFTL PSQTQTPPGG PGGPGGGGAG 
GPGGAGAGAA AGQLDAQVGP EGILQNGAVD DSVAKTSQLL AELNFEEDEE DTYYTKDLPI 
HACSYCGIHD PACVVYCNTS KKWFCNGRGN TSGSHIVNHL VRAKCKEVTL HKDGPLGETV 
LECYNCGCRN VFLLGFIPAK ADSVVVLLCR QPCASQSSLK DINWDSSQWQ PLIQDRCFLS 
WLVKIPSEQE QLRARQITAQ QINKLEELWK ENPSATLEDL EKPGVDEEPQ HVLLRYEDAY 
QYQNIFGPLV KLEADYDKKL KESQTQDNIT VRWDLGLNKK RIAYFTLPKT DSDMRLMQGD 
EICLRYKGDL APLWKGIGHV IKVPDNYGDE IAIELRSSVG APVEVTHNFQ VDFVWKSTSF 
DRMQSALKTF AVDETSVSGY IYHKLLGHEV EDVIIKCQLP KRFTAQGLPD LNHSQVYAVK 
TVLQRPLSLI QGPPGTGKTV TSATIVYHLA RQGNGPVLVC APSNIAVDQL TEKIHQTGLK 
VVRLCAKSRE AIDSPVSFLA LHNQIRNMDS MPELQKLQQL KDETGELSSA DEKRYRALKR 
TAERELLMNA DVICCTCVGA GDPRLAKMQF RSILIDESTQ ATEPECMVPV VLGAKQLILV 
GDHCQLGPVV MCKKAAKAGL SQSLFERLVV LGIRPIRLQV QYRMHPALSA FPSNIFYEGS 
LQNGVTAADR VKKGFDFQWP QPDKPMFFYV TQGQEEIASS GTSYLNRTEA ANVEKITTKL 
LKAGAKPDQI GIITPYEGQR SYLVQYMQFS GSLHTKLYQE VEIASVDAFQ GREKDFIILS 
CVRANEHQGI GFLNDPRRLN VALTRARYGV IIVGNPKALS KQPLWNHLLN YYKEQKVLVE 
GPLNNLRESL MQFSKPRKLV NTINPGARFM TTAMYDAREA IIPGSVYDRS SQGRPSSMYF 
QTHDQIGMIS AGPSHVAAMN IPIPFNLVMP PMPPPGYFGQ ANGPAAGRGT PKGKTGRGGR 
QKNRFGLPGP SQTNLPNSQA SQDVASQPFS QGALTQGYIS MSQPSQMSQP GLSQPELSQD 
SYLGDEFKSQ IDVALSQDST YQGERAYQHG GVTGLSQY

Genular Protein ID: 406002354

Symbol: B3KY55_HUMAN

Name: N/A

UniProtKB Accession Codes:

B3KY55

Database IDs:

AlphaFoldDB 1 BioGRID-ORCS 1 CDD 1 CTD 1 DNASU 1 DisGeNET 1 EMBL 2 GO 3 Gene3D 1 InterPro 4 KEGG 1 NCBI Taxonomy 1 OrthoDB 1 PANTHER 2 PeptideAtlas 1 Pfam 2 RefSeq 1 SAM 1 SUPFAM 1

Sequence Information:

  • Length: 373
  • Mass: 40970
  • Checksum: 2C3F3105EB48AA33
  • Sequence:
    • MFFYVTQGQE EIASSGTSYL NRTEAANVEK ITTKLLKAGA KPDQIGIITP YEGQRSYLVQ 
YMQFSGSLHT KLYQEVEIAS VDAFQGREKD FIILSCVRAN EHQGIGFLND PRRLNVALTR 
ARYGVIIVGN PKALSKQPLW NHLLNYYKEQ KVLVEGPLNN LRESLMQFSK PRKLVNTINP 
GARFMTTAMY DAREAIIPGS VYDRSSQGRP SSMYFQTHDQ IGMISAGPSH VAAMNIPIPF 
NLVMPPMPPP GYFGQANGPA AGRGTPKGKT GRGGRQKNRF GLPGPSQTNL PNSQASQDVA 
SQPFSQGALT QGYISMSQPS QMSQPGLSQP ELSQDSYLGD EFKSQIDVAL SQDSTYQGER 
AYQHGGVTGL SQY