Details for: ZNF814

Gene ID: 730051

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF814

Ensembl ID: ENSG00000204514

Description: zinc finger protein 814

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

  • Dna-binding transcription activator activity, rna polymerase ii-specific
    (GO:0001228)
  • Metal ion binding
    (GO:0046872)
  • Nucleus
    (GO:0005634)
  • Positive regulation of transcription by rna polymerase ii
    (GO:0045944)
  • Regulation of transcription by rna polymerase ii
    (GO:0006357)
  • Rna polymerase ii cis-regulatory region sequence-specific dna binding
    (GO:0000978)

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • choroid plexus epithelial cell CL0000706
    CSI 5.79
    rCSI 9.47%
    PRS 79.82
  • melanocyte CL0000148
    CSI 4.02
    rCSI 2.98%
    PRS 83.39
  • retina horizontal cell CL0000745
    CSI 4.01
    rCSI 6.11%
    PRS 85.04
  • regular atrial cardiac myocyte CL0002129
    CSI 3.97
    rCSI 12.78%
    PRS 84.58
  • kidney loop of Henle thin ascending limb epithelial cell CL1001107
    CSI 3.85
    rCSI 9.95%
    PRS 84.49
  • cerebellar granule cell CL0001031
    CSI 3.82
    rCSI 5.62%
    PRS 82.16
  • intestinal epithelial cell CL0002563
    CSI 3.78
    rCSI 3.95%
    PRS 85.56
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 3.59
    rCSI 13.57%
    PRS 73.65
  • Kupffer cell CL0000091
    CSI 3.46
    rCSI 7.92%
    PRS 88.91
  • brush cell of tracheobronchial tree CL0002075
    CSI 3.45
    rCSI 10.23%
    PRS 93.94
  • glioblast CL0000030
    CSI 3.38
    rCSI 5.39%
    PRS 80.18
  • interneuron CL0000099
    CSI 3.26
    rCSI 6.55%
    PRS 80.97
  • hematopoietic stem cell CL0000037
    CSI 3.25
    rCSI 2.16%
    PRS 89.85
  • lung secretory cell CL1000272
    CSI 3.25
    rCSI 8.04%
    PRS 88.23
  • naive B cell CL0000788
    CSI 3.19
    rCSI 2.73%
    PRS 91.76
  • hepatic stellate cell CL0000632
    CSI 2.91
    rCSI 10.91%
    PRS 82.47
  • Bergmann glial cell CL0000644
    CSI 2.87
    rCSI 3.92%
    PRS 79.92
  • neural crest cell CL0011012
    CSI 2.86
    rCSI 2.26%
    PRS 79.72
  • pro-B cell CL0000826
    CSI 2.75
    rCSI 2.27%
    PRS 89.92
  • radial glial cell CL0000681
    CSI 2.74
    rCSI 3.81%
    PRS 86.4
  • cardiac muscle cell CL0000746
    CSI 2.72
    rCSI 3.9%
    PRS 79.38
  • retinal rod cell CL0000604
    CSI 2.52
    rCSI 4.45%
    PRS 83.54
  • hepatocyte CL0000182
    CSI 2.49
    rCSI 4.46%
    PRS 86.94
  • lung ciliated cell CL1000271
    CSI 2.46
    rCSI 2.84%
    PRS 81.82
  • secretory cell CL0000151
    CSI 2.42
    rCSI 2.52%
    PRS 86.9
  • common dendritic progenitor CL0001029
    CSI 2.34
    rCSI 2.94%
    PRS 93.64
  • Mueller cell CL0000636
    CSI 2.33
    rCSI 5.31%
    PRS 80.84
  • adipocyte CL0000136
    CSI 2.32
    rCSI 2.98%
    PRS 79.01
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 2.32
    rCSI 2.98%
    PRS 84.47
  • sst GABAergic cortical interneuron CL4023017
    CSI 2.3
    rCSI 2.96%
    PRS 74.48
  • glial cell CL0000125
    CSI 2.27
    rCSI 8.65%
    PRS 80.74
  • cerebral cortex GABAergic interneuron CL0010011
    CSI 2.25
    rCSI 6.65%
    PRS 88.53
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 2.24
    rCSI 2.03%
    PRS 86.76
  • rod bipolar cell CL0000751
    CSI 2.24
    rCSI 4.02%
    PRS 82.45
  • mesothelial cell CL0000077
    CSI 2.22
    rCSI 8.69%
    PRS 69.32
  • common myeloid progenitor CL0000049
    CSI 2.22
    rCSI 1.79%
    PRS 89.66
  • blood vessel endothelial cell CL0000071
    CSI 2.22
    rCSI 4.6%
    PRS 85.8
  • ciliated epithelial cell CL0000067
    CSI 2.17
    rCSI 1.91%
    PRS 78.8
  • cardiac endothelial cell CL0010008
    CSI 2.16
    rCSI 8.71%
    PRS 88.39
  • common lymphoid progenitor CL0000051
    CSI 2.13
    rCSI 2.85%
    PRS 96.86
  • transit amplifying cell of colon CL0009011
    CSI 2.05
    rCSI 2.41%
    PRS 88.58
  • chondrocyte CL0000138
    CSI 2.05
    rCSI 3.26%
    PRS 82.59
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 1.99
    rCSI 2.48%
    PRS 71.18
  • glycinergic amacrine cell CL4030028
    CSI 1.96
    rCSI 5.1%
    PRS 82.04
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 1.94
    rCSI 7%
    PRS 71.35
  • neural cell CL0002319
    CSI 1.93
    rCSI 7.3%
    PRS 72.33
  • astrocyte of the cerebral cortex CL0002605
    CSI 1.92
    rCSI 4.3%
    PRS 74.05
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 1.91
    rCSI 2.2%
    PRS 80.68
  • VIP GABAergic cortical interneuron CL4023016
    CSI 1.88
    rCSI 2.24%
    PRS 73.53
  • BEST4+ enteroycte CL4030026
    CSI 1.82
    rCSI 2.27%
    PRS 87.77
  • kidney loop of Henle thin descending limb epithelial cell CL1001111
    CSI 1.79
    rCSI 2.53%
    PRS 85.33
  • peripheral nervous system neuron CL2000032
    CSI 1.77
    rCSI 2.41%
    PRS 81.23
  • renal beta-intercalated cell CL0002201
    CSI 1.77
    rCSI 4.22%
    PRS 87.79
  • retinal bipolar neuron CL0000748
    CSI 1.69
    rCSI 3.17%
    PRS 78.46
  • extravillous trophoblast CL0008036
    CSI 1.65
    rCSI 2.04%
    PRS 86.43
  • lymphoid lineage restricted progenitor cell CL0000838
    CSI 1.61
    rCSI 6.28%
    PRS 96.95
  • retinal pigment epithelial cell CL0002586
    CSI 1.59
    rCSI 3.16%
    PRS 84.13
  • direct pathway medium spiny neuron CL4023026
    CSI 1.58
    rCSI 37.86%
    PRS 70.97
  • pulmonary alveolar type 1 cell CL0002062
    CSI 1.57
    rCSI 9.04%
    PRS 84.62
  • fibroblast of cardiac tissue CL0002548
    CSI 1.56
    rCSI 7.49%
    PRS 88.4
  • epithelial cell of proximal tubule CL0002306
    CSI 1.54
    rCSI 3.76%
    PRS 80.89
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 1.44
    rCSI 2.54%
    PRS 72.83
  • lung pericyte CL0009089
    CSI 1.44
    rCSI 3.8%
    PRS 92.35
  • indirect pathway medium spiny neuron CL4023029
    CSI 1.43
    rCSI 34.62%
    PRS 71.39
  • retinal cone cell CL0000573
    CSI 1.34
    rCSI 2.15%
    PRS 79.62
  • glutamatergic neuron CL0000679
    CSI 1.31
    rCSI 2.69%
    PRS 75.81
  • amacrine cell CL0000561
    CSI 1.29
    rCSI 3.74%
    PRS 78.9
  • intestinal crypt stem cell of small intestine CL0009017
    CSI 1.24
    rCSI 3.33%
    PRS 90.58
  • L4 intratelencephalic projecting glutamatergic neuron CL4030063
    CSI 1.15
    rCSI 2.75%
    PRS 76.72
  • L6b glutamatergic cortical neuron CL4023038
    CSI 1.12
    rCSI 3.5%
    PRS 74.82
  • GABAergic neuron CL0000617
    CSI 1.1
    rCSI 3.7%
    PRS 73.68
  • kidney connecting tubule epithelial cell CL1000768
    CSI 1.1
    rCSI 2.79%
    PRS 80.66
  • neural progenitor cell CL0011020
    CSI 1.08
    rCSI 4.75%
    PRS 76.92
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 1.07
    rCSI 6.28%
    PRS 73.87
  • parietal epithelial cell CL1000452
    CSI 1.01
    rCSI 2.7%
    PRS 81.6
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 0.93
    rCSI 2.26%
    PRS 71.11
  • retinal ganglion cell CL0000740
    CSI 0.92
    rCSI 2.02%
    PRS 76.26
  • blood vessel smooth muscle cell CL0019018
    CSI 0.74
    rCSI 6.02%
    PRS 84.05
  • central nervous system neuron CL2000029
    CSI 0.66
    rCSI 4.83%
    PRS 78.04
  • regular ventricular cardiac myocyte CL0002131
    CSI 0.54
    rCSI 3.36%
    PRS 81.06
  • erythroid progenitor cell CL0000038
    CSI 0.54
    rCSI 3.08%
    PRS 90.74
  • ON midget ganglion cell CL4033046
    CSI 0.28
    rCSI 5.8%
    PRS 78.65

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF814](/details-gene/730051) is a protein-coding gene located on chromosome 19q13.43 that encodes Zinc Finger Protein 814. Functional annotations strongly suggest its role as a nuclear transcription factor involved in the positive regulation of transcription by RNA polymerase II ([GO:0045944](https://www.ebi.ac.uk/QuickGO/term/GO:0045944)). Its molecular function is characterized by sequence-specific DNA binding ([GO:0000978](https://www.ebi.ac.uk/QuickGO/term/GO:0000978)) and metal ion binding ([GO:0046872](https://www.ebi.ac.uk/QuickGO/term/GO:0046872)), consistent with its identity as a zinc finger protein. Expression data indicates its high significance in a diverse array of specialized cell types, most notably in [choroid plexus epithelial cell](/details-cell/CL0000706), suggesting it plays a critical role in establishing or maintaining the unique transcriptional programs of these cells. ## Cellular Roles and Expression Landscape The expression profile of [ZNF814](/details-gene/730051) indicates its importance across a wide variety of tissues and cell lineages, rather than being restricted to a single system. **Overall**, it shows the highest significance in [choroid plexus epithelial cell](/details-cell/CL0000706) (CSI: 5.79), a key component of the blood-cerebrospinal fluid barrier, implying a potential role in central nervous system homeostasis. The gene is also a significant marker in several other distinct cell types, which can be grouped into functional categories: * **Neural and Sensory Tissues:** High significance is observed in [melanocyte](/details-cell/CL0000148) (CSI: 4.02), [retina horizontal cell](/details-cell/CL0000745) (CSI: 4.01), [cerebellar granule cell](/details-cell/CL0001031) (CSI: 3.82), and [interneuron](/details-cell/CL0000099) (CSI: 3.26), suggesting a role in the development or function of the nervous and integumentary systems. * **Specialized Epithelial and Secretory Cells:** Beyond the choroid plexus, it is significant in [kidney loop of Henle thin ascending limb epithelial cell](/details-cell/CL1001107) (CSI: 3.85), [intestinal epithelial cell](/details-cell/CL0002563) (CSI: 3.78), [brush cell of tracheobronchial tree](/details-cell/CL0002075) (CSI: 3.45), and [lung secretory cell](/details-cell/CL1000272) (CSI: 3.25). This pattern points towards a function in regulating transport, barrier function, or secretion in diverse organs. * **Muscle and Immune Cells:** The gene also displays significance in [regular atrial cardiac myocyte](/details-cell/CL0002129) (CSI: 3.97), as well as immune-related cells like [Kupffer cell](/details-cell/CL0000091) (CSI: 3.46), [hematopoietic stem cell](/details-cell/CL0000037) (CSI: 3.25), and [naive B cell](/details-cell/CL0000788) (CSI: 3.19). This broad but cell-specific expression pattern suggests that [ZNF814](/details-gene/730051) is not a general housekeeping gene but rather a specific transcriptional regulator deployed to control distinct gene networks critical for the specialized identity and function of these varied cell types. ## Pathways and Molecular Function The functional annotations for [ZNF814](/details-gene/730051) define it as a DNA-binding transcription activator. Its activity is specific to RNA polymerase II-mediated transcription, as indicated by its association with [DNA-binding transcription activator activity, rna polymerase ii-specific](/details-go/GO:0001228) and [positive regulation of transcription by rna polymerase ii](/details-go/GO:0045944). The protein localizes to the [nucleus](/details-go/GO:0005634), where it can exert its regulatory effects. This function is consistent with its structural classification as a zinc finger protein, a large family of proteins known to bind DNA and regulate gene expression. While its direct downstream targets are not well characterized, its activity is likely regulated by post-translational modifications. Research has identified ZNF814 as a target of SUMOylation, a process that can modulate protein stability, localization, and activity ([Link](https://doi.org/10.1038/nsmb.2890), [Link](https://doi.org/10.1038/nsmb.3366)). This suggests that the transcriptional regulatory function of [ZNF814](/details-gene/730051) may be dynamically controlled in response to cellular signals. ## Research Directions The widespread but highly cell-type-specific significance of [ZNF814](/details-gene/730051) points to its role as a master regulator of specialized cellular functions. Further investigation is needed to elucidate its precise targets and regulatory networks in different contexts. **Proposed Testable Hypotheses:** 1. Given its top-ranking significance in [choroid plexus epithelial cell](/details-cell/CL0000706), [ZNF814](/details-gene/730051) is a critical transcription factor for establishing and maintaining the blood-cerebrospinal fluid barrier, regulating genes involved in ion transport and cerebrospinal fluid secretion. 2. The high significance of [ZNF814](/details-gene/730051) across diverse, terminally differentiated cells (e.g., [retina horizontal cell](/details-cell/CL0000745), [regular atrial cardiac myocyte](/details-cell/CL0002129), [melanocyte](/details-cell/CL0000148)) suggests it is essential for maintaining cellular identity and preventing dedifferentiation by actively regulating the expression of key functional genes specific to each lineage. **Suggested Experimental Approach:** To test the first hypothesis regarding the role of [ZNF814](/details-gene/730051) in the choroid plexus, an *in vitro* model using human choroid plexus organoids could be employed. CRISPR-Cas9-mediated knockout of [ZNF814](/details-gene/730051) in these organoids would allow for a multi-faceted analysis. The impact on barrier function could be directly measured by assessing transepithelial electrical resistance (TEER) and permeability to fluorescent tracers. Concurrently, single-cell RNA sequencing (scRNA-seq) of knockout versus control organoids would identify the downstream transcriptional targets of [ZNF814](/details-gene/730051) and reveal which cellular pathways related to barrier integrity and fluid secretion are disrupted. **Therapeutic Potential:** As an intracellular transcription factor, [ZNF814](/details-gene/730051) represents a challenging therapeutic target for conventional small molecules or antibodies. Its widespread, albeit specific, expression across numerous essential cell types raises a significant risk of off-target effects with systemic administration. Therefore, its potential as a therapeutic target is currently limited. Future therapeutic strategies, such as targeted gene editing or cell-type-specific delivery of RNA interference (RNAi) molecules, might be conceivable but require substantial technological advances. At present, it is more valuable as a research target for understanding the fundamental biology of specialized cells.

Genular Protein ID: 1156545309

Symbol: ZN814_HUMAN

Name: Zinc finger protein 814

UniProtKB Accession Codes:

B7Z6K7 A6NF35

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 3 Ensembl 1 ExpressionAtlas 1 GO 5 Gene3D 2 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 InterPro 4 KEGG 1 MANE-Select 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 RefSeq 1 SMART 2 SMR 1 STRING 1 SUPFAM 2 TopDownProteomics 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15057824

Title: The DNA sequence and biology of human chromosome 19.

PubMed ID: 15057824

DOI: 10.1038/nature02399

PubMed ID: 25218447

Title: Uncovering global SUMOylation signaling networks in a site-specific manner.

PubMed ID: 25218447

DOI: 10.1038/nsmb.2890

PubMed ID: 28112733

Title: Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.

PubMed ID: 28112733

DOI: 10.1038/nsmb.3366

Sequence Information:

  • Length: 855
  • Mass: 97337
  • Checksum: 7C497F30FCAACB17
  • Sequence:
    • MAAAATLRLS AQGTVTFEDV AVNFTWEEWN LLSEAQRCLY RDVTLENLAL ISSLGCWCGV 
EDEAAPSKQS IYIQRETQVR TPMAGVSPKK AHPCEMCGPI LGDILHVADH QGTHHKQKLH 
RCEAWGNKLY DSGNFHQHQN EHIGEKPYRG SVEEALFAKR CKLHVSGESS VFSESGKDFL 
PRSGLLQQEA SHTGEKSNSK TECVSPIQCG GAHYSCGESM KHFSTKHILS QHQRLLTREE 
CYVCCECGKS FSKYASLSNH QRVHTEKKHE CGECGKSFSK YVSFSNHQRV HTEKKHECGE 
CGKSFSKYVS FSNHQRVHTG KRPYECGECG KSFSKYASFS NHQRVHTEKK HYECGECGKS 
FSKYVSFSNH QRVHTGKRPY ECGECGKSFS KYASFSNHQR VHTDKKHYEC GECGKSFSQK 
SSLIQHQRFH TGEKPYGCEE CGKSFSSEGH LRSHQRVHAG ERPFKCGECV KSFSHKRSLV 
HHQRVHSGER PYQCGECGKS FSQKGNLVLH QRVHTGARPY ECGECGKSFS SKGHLRNHQQ 
IHTGDRLYEC GECGKSFSHK GTLILHQRVH PRERSYGCGE CGKSFSSIGH LRSHQRVHTG 
ERPYECGECG KSFSHKRSLV HHQRMHTGER PYKCGDCGKS FNEKGHLRNH QRVHTTERPF 
KCGECGKCFS HKGNLILHQH GHTGERPYVC RECGKLFKKK SHLLVHQRIH NGEKPYACEA 
CQKFFRNKYQ LIAHQRVHTG ERPYECNDCG KSFTHSSTFC VHKRIHTGEK PYECSECGKS 
FAESSSFTKH KRVHTGEKPY ECSECGKSFA ESSSLTKHKR VHTGEKPYKC EKCGKLFNKK 
SHLLVHQSSH WRKAI