Details for: WDR27

Gene ID: 253769

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: WDR27

Ensembl ID: ENSG00000184465

Description: WD repeat domain 27

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • choroid plexus epithelial cell CL0000706
    CSI 9.03
    rCSI 14.78%
    PRS 82.01
  • ependymal cell CL0000065
    CSI 8.86
    rCSI 17.98%
    PRS 71.46
  • erythroblast CL0000765
    CSI 8.04
    rCSI 21.35%
    PRS 91.02
  • kidney loop of Henle thin descending limb epithelial cell CL1001111
    CSI 6.72
    rCSI 9.52%
    PRS 87.02
  • sst GABAergic cortical interneuron CL4023017
    CSI 6.63
    rCSI 8.55%
    PRS 77.1
  • stromal cell CL0000499
    CSI 6.58
    rCSI 18.52%
    PRS 85.56
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 5.75
    rCSI 20.69%
    PRS 73.92
  • retinal bipolar neuron CL0000748
    CSI 5.67
    rCSI 10.62%
    PRS 80.67
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 5.22
    rCSI 6.5%
    PRS 73.81
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 5.17
    rCSI 12.57%
    PRS 73.71
  • mesothelial cell CL0000077
    CSI 5.04
    rCSI 19.7%
    PRS 72.12
  • VIP GABAergic cortical interneuron CL4023016
    CSI 4.81
    rCSI 5.75%
    PRS 75.97
  • inhibitory interneuron CL0000498
    CSI 4.51
    rCSI 10.42%
    PRS 80.2
  • fibroblast of cardiac tissue CL0002548
    CSI 4.14
    rCSI 19.83%
    PRS 90.28
  • pulmonary alveolar type 1 cell CL0002062
    CSI 4.09
    rCSI 23.55%
    PRS 86.43
  • cardiac endothelial cell CL0010008
    CSI 3.85
    rCSI 15.54%
    PRS 90.24
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 3.83
    rCSI 6.76%
    PRS 75.37
  • epithelial cell of proximal tubule CL0002306
    CSI 3.66
    rCSI 8.94%
    PRS 82.75
  • vascular leptomeningeal cell CL4023051
    CSI 3.51
    rCSI 6.16%
    PRS 85.74
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 3.41
    rCSI 12.9%
    PRS 76.21
  • sncg GABAergic cortical interneuron CL4023015
    CSI 3.37
    rCSI 5.42%
    PRS 77.05
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 3.32
    rCSI 19.55%
    PRS 76.48
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 3.28
    rCSI 5.5%
    PRS 75.92
  • L6b glutamatergic cortical neuron CL4023038
    CSI 3.23
    rCSI 10.1%
    PRS 77.36
  • naive B cell CL0000788
    CSI 3.2
    rCSI 2.74%
    PRS 93.32
  • kidney loop of Henle thin ascending limb epithelial cell CL1001107
    CSI 3.15
    rCSI 8.16%
    PRS 86.3
  • retinal cone cell CL0000573
    CSI 3.15
    rCSI 5.07%
    PRS 81.65
  • secretory cell CL0000151
    CSI 3.14
    rCSI 3.28%
    PRS 88.47
  • L4 intratelencephalic projecting glutamatergic neuron CL4030063
    CSI 2.9
    rCSI 6.94%
    PRS 78.82
  • Kupffer cell CL0000091
    CSI 2.89
    rCSI 6.6%
    PRS 90.51
  • kidney interstitial alternatively activated macrophage CL1000695
    CSI 2.84
    rCSI 7.39%
    PRS 90.88
  • respiratory suprabasal cell CL4033048
    CSI 2.78
    rCSI 3.57%
    PRS 91.31
  • common myeloid progenitor CL0000049
    CSI 2.74
    rCSI 2.21%
    PRS 90.98
  • astrocyte of the cerebral cortex CL0002605
    CSI 2.71
    rCSI 6.08%
    PRS 76.41
  • hepatic stellate cell CL0000632
    CSI 2.65
    rCSI 9.94%
    PRS 84.6
  • kidney connecting tubule epithelial cell CL1000768
    CSI 2.65
    rCSI 6.71%
    PRS 82.86
  • interneuron CL0000099
    CSI 2.59
    rCSI 5.2%
    PRS 83.14
  • retinal rod cell CL0000604
    CSI 2.59
    rCSI 4.56%
    PRS 85.51
  • mesodermal cell CL0000222
    CSI 2.55
    rCSI 3.06%
    PRS 88.31
  • chondrocyte CL0000138
    CSI 2.53
    rCSI 4.02%
    PRS 84.65
  • endocardial cell CL0002350
    CSI 2.51
    rCSI 12%
    PRS 85.84
  • alveolar type 1 fibroblast cell CL4028004
    CSI 2.44
    rCSI 2.67%
    PRS 91.05
  • Mueller cell CL0000636
    CSI 2.43
    rCSI 5.54%
    PRS 82.83
  • blood vessel endothelial cell CL0000071
    CSI 2.4
    rCSI 4.99%
    PRS 87.73
  • intestinal epithelial cell CL0002563
    CSI 2.38
    rCSI 2.49%
    PRS 87.17
  • cerebral cortex endothelial cell CL1001602
    CSI 2.37
    rCSI 4.1%
    PRS 83.93
  • cardiac neuron CL0010022
    CSI 2.31
    rCSI 7.38%
    PRS 88
  • ciliated epithelial cell CL0000067
    CSI 2.21
    rCSI 1.95%
    PRS 80.68
  • ciliated cell CL0000064
    CSI 2.17
    rCSI 3.52%
    PRS 83.79
  • stem cell CL0000034
    CSI 2.07
    rCSI 1.99%
    PRS 85.2
  • adipocyte CL0000136
    CSI 2.06
    rCSI 2.64%
    PRS 81.29
  • renal beta-intercalated cell CL0002201
    CSI 2.03
    rCSI 4.84%
    PRS 89.41
  • renal interstitial pericyte CL1001318
    CSI 2.02
    rCSI 5.58%
    PRS 86.56
  • Bergmann glial cell CL0000644
    CSI 1.99
    rCSI 2.72%
    PRS 81.83
  • podocyte CL0000653
    CSI 1.95
    rCSI 8.68%
    PRS 90.04
  • direct pathway medium spiny neuron CL4023026
    CSI 1.92
    rCSI 45.97%
    PRS 73.71
  • renal principal cell CL0005009
    CSI 1.91
    rCSI 4.97%
    PRS 88.75
  • retinal ganglion cell CL0000740
    CSI 1.9
    rCSI 4.2%
    PRS 78.61
  • multi-ciliated epithelial cell CL0005012
    CSI 1.89
    rCSI 1.88%
    PRS 83.8
  • indirect pathway medium spiny neuron CL4023029
    CSI 1.88
    rCSI 45.4%
    PRS 73.94
  • alveolar adventitial fibroblast CL4028006
    CSI 1.84
    rCSI 2.91%
    PRS 90.44
  • lung secretory cell CL1000272
    CSI 1.8
    rCSI 4.45%
    PRS 90.03
  • amacrine cell CL0000561
    CSI 1.77
    rCSI 5.13%
    PRS 80.91
  • lung ciliated cell CL1000271
    CSI 1.72
    rCSI 1.98%
    PRS 83.77
  • extravillous trophoblast CL0008036
    CSI 1.61
    rCSI 2%
    PRS 87.97
  • parietal epithelial cell CL1000452
    CSI 1.47
    rCSI 3.92%
    PRS 83.77
  • ciliated columnar cell of tracheobronchial tree CL0002145
    CSI 1.44
    rCSI 3.28%
    PRS 82.78
  • GABAergic neuron CL0000617
    CSI 1.42
    rCSI 4.77%
    PRS 75.89
  • lymphoid lineage restricted progenitor cell CL0000838
    CSI 1.42
    rCSI 5.52%
    PRS 97.5
  • S cone cell CL0003050
    CSI 1.34
    rCSI 5.89%
    PRS 84.85
  • chandelier pvalb GABAergic cortical interneuron CL4023036
    CSI 1.18
    rCSI 3.7%
    PRS 79.14
  • invaginating midget bipolar cell CL4033034
    CSI 1.05
    rCSI 6.18%
    PRS 80.94
  • kidney loop of Henle thick ascending limb epithelial cell CL1001106
    CSI 1
    rCSI 8.64%
    PRS 84.01
  • ON midget ganglion cell CL4033046
    CSI 0.72
    rCSI 14.68%
    PRS 80.79
  • glial cell CL0000125
    CSI 0.72
    rCSI 2.73%
    PRS 82.79
  • kidney distal convoluted tubule epithelial cell CL1000849
    CSI 0.68
    rCSI 7.17%
    PRS 85.52
  • blood vessel smooth muscle cell CL0019018
    CSI 0.64
    rCSI 5.17%
    PRS 86.39
  • diffuse bipolar 3a cell CL4033029
    CSI 0.56
    rCSI 3.81%
    PRS 81.68
  • OFF midget ganglion cell CL4033047
    CSI 0.48
    rCSI 9.83%
    PRS 81.56
  • ON parasol ganglion cell CL4033052
    CSI 0.47
    rCSI 6.72%
    PRS 81.87

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [WDR27](/details-gene/253769) is a protein-coding gene located on chromosome 6q27 that encodes WD repeat domain 27, a protein characterized by WD repeats which typically mediate protein-protein interactions [Link](https://doi.org/10.1038/nature02055). Functional annotations suggest [WDR27](/details-gene/253769) operates as a protein-binding scaffold within the nucleoplasm. Expression data highlights its significant role in specialized cells of the central nervous system, particularly the [choroid plexus epithelial cell](/details-cell/CL0000706) and [ependymal cell](/details-cell/CL0000065) that line the brain's ventricles. Additionally, its high expression in developing [erythroblast](/details-cell/CL0000765)s suggests a potential function in hematopoietic cell differentiation. The initial characterization of this gene was part of large-scale cDNA and genome sequencing projects [Link](https://doi.org/10.1101/gr.2596504). ## Cellular Roles and Expression Landscape **Overall**, the expression profile of [WDR27](/details-gene/253769) indicates a broad but distinct pattern of cellular importance, with the highest significance observed in specific neural and hematopoietic lineages. The most prominent expression is within the central nervous system, specifically in cell types associated with the production and circulation of cerebrospinal fluid (CSF). It is a top marker in [choroid plexus epithelial cell](/details-cell/CL0000706) (CSI: 9.03), which are responsible for CSF secretion, and [ependymal cell](/details-cell/CL0000065) (CSI: 8.86), which line the ventricles and facilitate CSF movement. Beyond the ventricular system, [WDR27](/details-gene/253769) shows significant expression across a diverse array of neuronal subtypes, including both inhibitory neurons like [sst GABAergic cortical interneuron](/details-cell/CL4023017) and [pvalb GABAergic cortical interneuron](/details-cell/CL4023018), and excitatory neurons such as [L5 extratelencephalic projecting glutamatergic cortical neuron](/details-cell/CL4023041). This suggests a widespread, potentially fundamental role in neuronal nuclear function. A second major site of high [WDR27](/details-gene/253769) significance is the hematopoietic system, where it is highly expressed in [erythroblast](/details-cell/CL0000765)s (CSI: 8.04). These cells are red blood cell precursors undergoing terminal differentiation, a process involving dramatic nuclear condensation and eventual enucleation. The gene's elevated expression in this context points towards a role in orchestrating these complex nuclear events. The gene is also moderately expressed in various epithelial, stromal, and mesenchymal cell types, such as [kidney loop of Henle thin descending limb epithelial cell](/details-cell/CL1001111), [stromal cell](/details-cell/CL0000499), and [mesothelial cell](/details-cell/CL0000077), indicating a broader, though less specific, function in these tissues. ## Pathways and Molecular Function The known molecular function of [WDR27](/details-gene/253769) is protein binding ([GO:0005515](https://www.ebi.ac.uk/QuickGO/term/GO:0005515)), and it is localized to the nucleoplasm ([GO:0005654](https://www.ebi.ac.uk/QuickGO/term/GO:0005654)). This is consistent with its structural WD repeat domains, which act as platforms for the assembly of protein complexes. This function as a nuclear scaffold protein can be contextualized by its cellular expression patterns. * In the diverse neuronal subtypes, [WDR27](/details-gene/253769) may serve as a hub for transcriptional regulatory complexes that define or maintain specific neuronal identities. * In [choroid plexus epithelial cell](/details-cell/CL0000706)s and [ependymal cell](/details-cell/CL0000065)s, it could be involved in regulating the expression of genes essential for ion transport, fluid secretion, or ciliogenesis. * In [erythroblast](/details-cell/CL0000765)s, its protein-binding function in the nucleus is highly suggestive of a role in organizing the protein machinery required for the extensive chromatin remodeling and condensation that precedes enucleation. ## Research Directions The specific expression patterns of [WDR27](/details-gene/253769) invite further investigation into its precise biological roles, particularly in neurology and hematology. ### Proposed Hypotheses 1. **Role in CNS Fluid Homeostasis:** The high significance of [WDR27](/details-gene/253769) in [choroid plexus epithelial cell](/details-cell/CL0000706)s and [ependymal cell](/details-cell/CL0000065)s suggests that it is a key nuclear regulator of gene expression programs controlling cerebrospinal fluid (CSF) production and/or ciliary function required for CSF circulation. 2. **Function in Terminal Erythropoiesis:** The prominent expression in [erythroblast](/details-cell/CL0000765)s indicates that [WDR27](/details-gene/253769) functions as a critical scaffold protein in the nucleus, assembling protein complexes necessary for the orderly chromatin condensation and nuclear pyknosis that are hallmarks of terminal red blood cell differentiation. ### Key Experimental Approach To test the hypothesis regarding its role in erythropoiesis, a compelling experiment would be to use a CRISPR-Cas9-based approach to knock out [WDR27](/details-gene/253769) in primary human hematopoietic stem and progenitor cells (HSPCs). These engineered HSPCs would then be differentiated *in vitro* into the erythroid lineage. The progression of differentiation would be assessed via flow cytometry for key surface markers (e.g., CD71, Glycophorin A) and detailed imaging analysis (e.g., confocal microscopy with nuclear staining) to quantify rates of enucleation and assess nuclear morphology. A significant defect in nuclear condensation or a reduction in the percentage of enucleated cells in the knockout condition compared to wild-type controls would provide strong evidence for the gene's essential role in this process. ### Therapeutic Potential Based on current data, [WDR27](/details-gene/253769) does not present as an immediate high-priority therapeutic target. Its function as an intracellular nuclear protein makes it challenging to target with conventional biologics like antibodies. Furthermore, its expression in a variety of critical cell types, including diverse neurons and hematopoietic precursors, raises the possibility of significant on-target toxicity if systemically inhibited. Any therapeutic consideration would first require a much deeper understanding of its specific interaction partners and its potential links to pathology, such as developmental brain disorders or specific anemias.

Genular Protein ID: 107977571

Symbol: WDR27_HUMAN

Name: WD repeat-containing protein 27

UniProtKB Accession Codes:

A2RRH5 A5PLM8 C9JGV0 Q5T066

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 2 CTD 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 7 Ensembl 2 ExpressionAtlas 1 GO 1 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 4 KEGG 1 MANE-Select 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 RNAct 1 RefSeq 2 SMART 1 STRING 1 SUPFAM 1 SignaLink 1 TopDownProteomics 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 neXtProt 1

Citations:

PubMed ID: 14574404

Title: The DNA sequence and analysis of human chromosome 6.

PubMed ID: 14574404

DOI: 10.1038/nature02055

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 827
  • Mass: 90038
  • Checksum: 932404623943AC4C
  • Sequence:
    • MENPQDIFSS NGGCLSDIVI EKYLVESKES VSHVQLACSM QDCAFPLDGT ELCIWNTKDP 
SHQLLILRGH HQPITAMAFG NKVNPLLICS ASLDYVIMWN LDECREKVLQ GLVPRGTVMG 
SLLGKVLCLQ LSLDDHVVAV CAGNKIFMLD IETQAVRAEL QGHLGPVTAV EFCPWRAGTL 
ISASEDRGFK VWDHCTGSLI YSSSVLSAYP LLSLFIDAES RQLVTGCADG QLWIFSLMDG 
HHYRRVARVD LRKKTETFST RRVKSGLCSQ PEESQLPSTS ALGKGEQVEV TFPVLRLAPC 
DLSLIPNSAC GCLSSENTRC VWIGSSVGLF VFNLANLEVE AALYYKDFQS LSILLAGSCA 
LRNRTADQKV LCLLASLFGG KIAVLEINPA ALVRAQQCPS MGQSLSVPAS SCVLPTSPLY 
LGIAKEKSTK AASEQRRAAR NVMKDQRLVF HSKVRSSGYA SAPHVTMFSP KTNIKSEGKG 
SSRSRSSCAR EAYPVECAVP TKPGPQVAAA PTCTRVCCIQ YSGDGQWLAC GLANHLLLVF 
DASLTGTPAV FSGHDGAVNA VCWSQDRRWL LSAARDGTLR MWSARGAELA LLLGKDMFSK 
PIQSAQFYYI DAFILLSSGP EFQLLRYHID TCKDEIKRYK QKSKSKLICR LSTTGAVDMT 
SLSAVNDFYS HIVLAAGRNR TVEVFDLNAG CSAAVIAEAH SRPVHQICQN KGSSFTTQQP 
QAYNLFLTTA IGDGMRLWDL RTLRCERHFE GHPTRGYPCG IAFSPCGRFA ACGAEDRHAY 
VYEMGSSTFS HRLAGHTDTV TGVAFNPSAP QLATATLDGK LQLFLAE