Details for: PTK7

Gene ID: 5754

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: PTK7

Ensembl ID: ENSG00000112655

Description: protein tyrosine kinase 7 (inactive)

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • glial cell CL0000125
    CSI 5.54
    rCSI 21.09%
    PRS 72.88
  • ionocyte CL0005006
    CSI 5.08
    rCSI 5.45%
    PRS 82.9
  • alveolar adventitial fibroblast CL4028006
    CSI 4.12
    rCSI 6.51%
    PRS 82.93
  • astrocyte of the cerebral cortex CL0002605
    CSI 3.98
    rCSI 8.92%
    PRS 64.87
  • skin fibroblast CL0002620
    CSI 3.6
    rCSI 3.1%
    PRS 82.29
  • pancreatic stellate cell CL0002410
    CSI 3.57
    rCSI 20.8%
    PRS 85.02
  • plasmacytoid dendritic cell, human CL0001058
    CSI 3.32
    rCSI 2.31%
    PRS 84.92
  • pancreatic D cell CL0000173
    CSI 3.17
    rCSI 3.11%
    PRS 83.82
  • interstitial cell of Cajal CL0002088
    CSI 3.14
    rCSI 4%
    PRS 86.39
  • choroid plexus epithelial cell CL0000706
    CSI 2.98
    rCSI 4.87%
    PRS 71.33
  • respiratory basal cell CL0002633
    CSI 2.88
    rCSI 2.98%
    PRS 85.27
  • secretory cell CL0000151
    CSI 2.83
    rCSI 2.95%
    PRS 80.9
  • intestinal epithelial cell CL0002563
    CSI 2.78
    rCSI 2.91%
    PRS 79.05
  • myoepithelial cell CL0000185
    CSI 2.78
    rCSI 7.03%
    PRS 86.18
  • bronchus fibroblast of lung CL2000093
    CSI 2.75
    rCSI 2.23%
    PRS 80.91
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 2.72
    rCSI 3.49%
    PRS 77.69
  • stem cell CL0000034
    CSI 2.71
    rCSI 2.62%
    PRS 75.21
  • renal beta-intercalated cell CL0002201
    CSI 2.7
    rCSI 6.44%
    PRS 81.57
  • lung ciliated cell CL1000271
    CSI 2.66
    rCSI 3.07%
    PRS 74.04
  • ciliated epithelial cell CL0000067
    CSI 2.66
    rCSI 2.34%
    PRS 71.03
  • fibroblast of lung CL0002553
    CSI 2.65
    rCSI 2.46%
    PRS 82.27
  • radial glial cell CL0000681
    CSI 2.64
    rCSI 3.67%
    PRS 79.9
  • neural crest cell CL0011012
    CSI 2.64
    rCSI 2.08%
    PRS 70.89
  • blood vessel endothelial cell CL0000071
    CSI 2.64
    rCSI 5.47%
    PRS 78.38
  • kidney loop of Henle thin descending limb epithelial cell CL1001111
    CSI 2.61
    rCSI 3.7%
    PRS 78.36
  • alveolar type 1 fibroblast cell CL4028004
    CSI 2.58
    rCSI 2.83%
    PRS 83.38
  • epithelial cell of lung CL0000082
    CSI 2.52
    rCSI 2.09%
    PRS 82.38
  • pancreatic A cell CL0000171
    CSI 2.46
    rCSI 2.58%
    PRS 84.45
  • vascular leptomeningeal cell CL4023051
    CSI 2.44
    rCSI 4.28%
    PRS 75.93
  • retinal pigment epithelial cell CL0002586
    CSI 2.43
    rCSI 4.83%
    PRS 77.31
  • interneuron CL0000099
    CSI 2.4
    rCSI 4.82%
    PRS 72.27
  • epithelial cell of lower respiratory tract CL0002632
    CSI 2.37
    rCSI 1.84%
    PRS 85.18
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 2.34
    rCSI 2.92%
    PRS 61.94
  • conjunctival epithelial cell CL1000432
    CSI 2.34
    rCSI 3.57%
    PRS 81.65
  • myofibroblast cell CL0000186
    CSI 2.3
    rCSI 3.18%
    PRS 78.6
  • basal-myoepithelial cell of mammary gland CL0002324
    CSI 2.26
    rCSI 4.28%
    PRS 91.1
  • enteroendocrine cell CL0000164
    CSI 2.17
    rCSI 2.97%
    PRS 81.07
  • stromal cell of ovary CL0002132
    CSI 2.16
    rCSI 5.93%
    PRS 87.7
  • ependymal cell CL0000065
    CSI 2.15
    rCSI 4.37%
    PRS 60.43
  • Mueller cell CL0000636
    CSI 2.08
    rCSI 4.76%
    PRS 73.22
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 2.04
    rCSI 2.36%
    PRS 73.76
  • respiratory hillock cell CL4030023
    CSI 2.02
    rCSI 3.61%
    PRS 89.41
  • respiratory suprabasal cell CL4033048
    CSI 1.98
    rCSI 2.53%
    PRS 84.53
  • fallopian tube secretory epithelial cell CL4030006
    CSI 1.95
    rCSI 1.87%
    PRS 80.84
  • enteric smooth muscle cell CL0002504
    CSI 1.9
    rCSI 2.72%
    PRS 82.27
  • mesodermal cell CL0000222
    CSI 1.86
    rCSI 2.23%
    PRS 79.56
  • lung pericyte CL0009089
    CSI 1.84
    rCSI 4.85%
    PRS 87.69
  • glioblast CL0000030
    CSI 1.8
    rCSI 2.87%
    PRS 73.17
  • pulmonary alveolar type 2 cell CL0002063
    CSI 1.79
    rCSI 2.77%
    PRS 85.1
  • multi-ciliated epithelial cell CL0005012
    CSI 1.78
    rCSI 1.78%
    PRS 75.47
  • basal cell CL0000646
    CSI 1.78
    rCSI 2.38%
    PRS 79.83
  • intestinal tuft cell CL0019032
    CSI 1.78
    rCSI 2.72%
    PRS 84.93
  • sncg GABAergic cortical interneuron CL4023015
    CSI 1.77
    rCSI 2.85%
    PRS 65.6
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 1.74
    rCSI 1.57%
    PRS 79.67
  • peripheral nervous system neuron CL2000032
    CSI 1.71
    rCSI 2.32%
    PRS 73.39
  • L4 intratelencephalic projecting glutamatergic neuron CL4030063
    CSI 1.68
    rCSI 4.03%
    PRS 68.53
  • kidney loop of Henle thin ascending limb epithelial cell CL1001107
    CSI 1.65
    rCSI 4.25%
    PRS 77.07
  • smooth muscle cell CL0000192
    CSI 1.61
    rCSI 3.84%
    PRS 77.56
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 1.59
    rCSI 2.81%
    PRS 63.52
  • chondrocyte CL0000138
    CSI 1.56
    rCSI 2.48%
    PRS 74.91
  • vascular associated smooth muscle cell CL0000359
    CSI 1.54
    rCSI 4.98%
    PRS 79.65
  • lung secretory cell CL1000272
    CSI 1.45
    rCSI 3.58%
    PRS 81.59
  • placental villous trophoblast CL2000060
    CSI 1.36
    rCSI 2.11%
    PRS 80.65
  • type B pancreatic cell CL0000169
    CSI 1.32
    rCSI 2.92%
    PRS 80.85
  • inhibitory interneuron CL0000498
    CSI 1.25
    rCSI 2.9%
    PRS 69.84
  • stromal cell CL0000499
    CSI 1.2
    rCSI 3.38%
    PRS 76.76
  • amacrine cell CL0000561
    CSI 1.16
    rCSI 3.37%
    PRS 71.29
  • retinal cone cell CL0000573
    CSI 1.15
    rCSI 1.85%
    PRS 71.95
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 1.12
    rCSI 1.88%
    PRS 64.06
  • L6b glutamatergic cortical neuron CL4023038
    CSI 1.09
    rCSI 3.4%
    PRS 65.73
  • mesenchymal cell CL0008019
    CSI 1.06
    rCSI 2.7%
    PRS 75.15
  • retinal ganglion cell CL0000740
    CSI 1.03
    rCSI 2.27%
    PRS 67.7
  • kidney connecting tubule epithelial cell CL1000768
    CSI 1.01
    rCSI 2.56%
    PRS 72.24
  • neural progenitor cell CL0011020
    CSI 0.97
    rCSI 4.25%
    PRS 70.12
  • ON midget ganglion cell CL4033046
    CSI 0.96
    rCSI 19.57%
    PRS 71.47
  • podocyte CL0000653
    CSI 0.93
    rCSI 4.15%
    PRS 82.11
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 0.86
    rCSI 2.08%
    PRS 62.07
  • mesenchymal stem cell CL0000134
    CSI 0.78
    rCSI 8.57%
    PRS 86.51
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 0.77
    rCSI 4.53%
    PRS 64.74
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 0.63
    rCSI 2.37%
    PRS 64.66
  • central nervous system neuron CL2000029
    CSI 0.45
    rCSI 3.29%
    PRS 69.37
  • OFF midget ganglion cell CL4033047
    CSI 0.32
    rCSI 6.6%
    PRS 72.58
  • pluripotent stem cell CL0002248
    CSI 0.17
    rCSI 5.01%
    PRS 90.67

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [PTK7](/details-gene/5754) (Protein Tyrosine Kinase 7) is a member of the receptor tyrosine kinase family that is characterized as being catalytically inactive or a 'pseudokinase'. Despite the lack of intrinsic kinase activity, it functions as a critical cell surface co-receptor involved in a diverse range of cellular processes, including cell migration, adhesion, and planar cell polarity. It is a key modulator of the canonical Wnt signaling pathway. **Overall**, expression data indicates that [PTK7](/details-gene/5754) is a significant marker in various cell types, with particularly high significance in [glial cell](/details-cell/CL0000125) populations, multiple types of fibroblasts and stellate cells, and specialized secretory cells. Its involvement in fundamental developmental pathways and its aberrant expression in certain cancers underscore its biological and clinical importance. An association with a clinical phenotype is noted in OMIM ([601890](https://omim.org/entry/601890)). ## Cellular Roles and Expression Landscape The expression profile of [PTK7](/details-gene/5754) suggests a widespread but functionally distinct role across multiple tissue lineages. Its significance is highest in neural support cells and mesenchymal cell types. **Overall**, the top ranked cell types include [glial cell](/details-cell/CL0000125) (CSI: 5.54) and [astrocyte of the cerebral cortex](/details-cell/CL0002605) (CSI: 3.98), highlighting a potential role in the central nervous system. Furthermore, [PTK7](/details-gene/5754) is a prominent marker in several fibroblast and stromal cell populations, including [alveolar adventitial fibroblast](/details-cell/CL4028006) (CSI: 4.12), [skin fibroblast](/details-cell/CL0002620) (CSI: 3.60), and [pancreatic stellate cell](/details-cell/CL0002410) (CSI: 3.57). This pattern is consistent with its function in organizing the actin cytoskeleton and mediating cell adhesion. The gene also shows significant expression in various specialized epithelial and secretory cells such as [ionocyte](/details-cell/CL0005006) (CSI: 5.08), [choroid plexus epithelial cell](/details-cell/CL0000706) (CSI: 2.98), and [pancreatic D cell](/details-cell/CL0000173) (CSI: 3.17), implying a role in tissue morphogenesis and cell polarity. While predominantly expressed in non-hematopoietic lineages, its notable significance in [plasmacytoid dendritic cell, human](/details-cell/CL0001058) (CSI: 3.32) suggests a potential, albeit less characterized, function in the immune system. ## Pathways and Molecular Function Functionally, [PTK7](/details-gene/5754) is annotated to a broad spectrum of biological processes centered on development, cell motility, and signaling. It is integral to morphogenesis and development, with roles in [axis elongation](/details-cell/GO:0003401), [convergent extension](/details-cell/GO:0060026), and the development of numerous organ systems including the [kidney](/details-cell/GO:0001822), [coronary vasculature](/details-cell/GO:0060976), and nervous system ([axon guidance](/details-cell/GO:0007411)). As a cell surface molecule localized to the [plasma membrane](/details-cell/GO:0005886) and [cell-cell junction](/details-cell/GO:0005911), a primary function of [PTK7](/details-gene/5754) is the establishment and maintenance of cell polarity, specifically [planar polarity](/details-cell/GO:0001736) and [epithelial cell apical/basal polarity](/details-cell/GO:0045198). This is mediated through its molecular functions in [homophilic cell adhesion](/details-cell/GO:0007156) and [protein binding](/details-cell/GO:0005515). Critically, [PTK7](/details-gene/5754) acts as a co-receptor in the Wnt signaling pathway, where it is involved in the [positive regulation of canonical wnt signaling pathway](/details-cell/GO:0090263) ([Link](https://doi.org/10.1038/embor.2010.185)). Its pseudokinase domain, while catalytically inactive, is thought to mediate these protein-protein interactions, transducing signals that influence the [actin cytoskeleton organization](/details-cell/GO:0030036) to drive processes like [cell migration](/details-cell/GO:0016477). ## Research Directions The diverse roles of [PTK7](/details-gene/5754) in development and its aberrant expression in disease, as noted in several publications ([Link](https://doi.org/10.1182/blood-2010-01-262352), [Link](https://doi.org/10.1371/journal.pone.0014018)), present several avenues for future investigation. Its high expression in stromal cells and its function in cell migration are particularly relevant in the context of the tumor microenvironment. **Proposed Hypotheses:** 1. Given its high significance in fibroblast populations and its role in Wnt signaling and cell migration, [PTK7](/details-gene/5754) may be a key regulator in the activation of cancer-associated fibroblasts (CAFs). Upregulation of [PTK7](/details-gene/5754) in stromal fibroblasts, in response to tumor-secreted factors, could promote their transition to a pro-invasive, pro-angiogenic CAF phenotype. 2. Based on its high expression in [glial cell](/details-cell/CL0000125) and [astrocyte of the cerebral cortex](/details-cell/CL0002605) and its annotated function as an [axon guidance receptor](/details-cell/GO:0008046), [PTK7](/details-gene/5754) likely acts as a co-receptor on astrocytes that binds to specific neuronal ligands, thereby guiding migrating neurons and stabilizing synaptic connections during brain development and plasticity. **Experimental Approach:** To test the first hypothesis regarding the role of [PTK7](/details-gene/5754) in CAF activation, one could perform a co-culture experiment. Primary human fibroblasts (e.g., from lung or skin tissue) would be transduced with lentiviral vectors for CRISPR-Cas9-mediated knockout of [PTK7](/details-gene/5754) or a non-targeting control. These engineered fibroblasts would then be co-cultured with a relevant cancer cell line (e.g., A549 lung carcinoma). The activation state of the fibroblasts could be assessed by measuring the expression of CAF markers like alpha-smooth muscle actin (α-SMA) and fibroblast activation protein (FAP) via immunofluorescence and quantitative PCR. Furthermore, the functional impact on cancer cell behavior could be measured using transwell migration and invasion assays with conditioned media from the co-cultures. **Therapeutic Potential:** As a cell surface protein with altered expression in several malignancies, including acute myeloid leukemia ([Link](https://doi.org/10.1182/blood-2010-01-262352)), [PTK7](/details-gene/5754) represents a promising therapeutic target. Its accessibility on the plasma membrane makes it suitable for antibody-based therapies. The therapeutic strategy would focus on **inhibition** or targeted cell killing. For hematological cancers like AML where high [PTK7](/details-gene/5754) expression correlates with poor outcomes, it could be an excellent target for an antibody-drug conjugate (ADC) or for chimeric antigen receptor (CAR)-T cell therapy to specifically eliminate the malignant cell population.

Genular Protein ID: 821378831

Symbol: PTK7_HUMAN

Name: Inactive tyrosine-protein kinase 7

UniProtKB Accession Codes:

Q13308 A8K974 B7Z477 E9PFZ5 Q13417 Q5T650 Q6IQ54 Q8NFA5 Q8NFA6 Q8NFA7 Q8NFA8

Database IDs:

ABCD 1 AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 5 CDD 3 CTD 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 28 Ensembl 6 ExpressionAtlas 1 GO 29 Gene3D 2 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyConnect 1 GlyCosmos 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 11 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 MetOSite 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 1 PDBsum 1 PIR 1 PRINTS 1 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 3 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 6 Pumba 1 RNAct 1 RefSeq 5 SIGNOR 1 SMART 2 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 SwissPalm 1 TopDownProteomics 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 2 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 7478540

Title: Colon carcinoma kinase-4 defines a new subclass of the receptor tyrosine kinase family.

PubMed ID: 7478540

PubMed ID: 8882711

Title: Characterization of the human full-length PTK7 cDNA encoding a receptor protein tyrosine kinase-like molecule closely related to chick KLG.

PubMed ID: 8882711

DOI: 10.1093/oxfordjournals.jbchem.a021228

PubMed ID: 12427550

Title: Organization of the human PTK7 gene encoding a receptor protein tyrosine kinase-like molecule and alternative splicing of its mRNA.

PubMed ID: 12427550

DOI: 10.1016/s0167-4781(02)00536-5

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 14574404

Title: The DNA sequence and analysis of human chromosome 6.

PubMed ID: 14574404

DOI: 10.1038/nature02055

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 12754519

Title: Identification and quantification of N-linked glycoproteins using hydrazide chemistry, stable isotope labeling and mass spectrometry.

PubMed ID: 12754519

DOI: 10.1038/nbt827

PubMed ID: 19159218

Title: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry.

PubMed ID: 19159218

DOI: 10.1021/pr8008012

PubMed ID: 18471990

Title: Soluble PTK7 inhibits tube formation, migration, and invasion of endothelial cells and angiogenesis.

PubMed ID: 18471990

DOI: 10.1016/j.bbrc.2008.04.168

PubMed ID: 19349973

Title: Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins.

PubMed ID: 19349973

DOI: 10.1038/nbt.1532

PubMed ID: 20558616

Title: The cell polarity PTK7 receptor acts as a modulator of the chemotherapeutic response in acute myeloid leukemia and impairs clinical outcome.

PubMed ID: 20558616

DOI: 10.1182/blood-2010-01-262352

PubMed ID: 20837484

Title: The Wnt/planar cell polarity protein-tyrosine kinase-7 (PTK7) is a highly efficient proteolytic target of membrane type-1 matrix metalloproteinase: implications in cancer and embryogenesis.

PubMed ID: 20837484

DOI: 10.1074/jbc.m110.165159

PubMed ID: 21103379

Title: Silencing of PTK7 in colon cancer cells: caspase-10-dependent apoptosis via mitochondrial pathway.

PubMed ID: 21103379

DOI: 10.1371/journal.pone.0014018

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 21132015

Title: Protein tyrosine kinase 7 has a conserved role in Wnt/beta-catenin canonical signalling.

PubMed ID: 21132015

DOI: 10.1038/embor.2010.185

PubMed ID: 17344846

Title: Patterns of somatic mutation in human cancer genomes.

PubMed ID: 17344846

DOI: 10.1038/nature05610

Sequence Information:

  • Length: 1070
  • Mass: 118392
  • Checksum: 304926A1774EB5F4
  • Sequence:
    • MGAARGSPAR PRRLPLLSVL LLPLLGGTQT AIVFIKQPSS QDALQGRRAL LRCEVEAPGP 
VHVYWLLDGA PVQDTERRFA QGSSLSFAAV DRLQDSGTFQ CVARDDVTGE EARSANASFN 
IKWIEAGPVV LKHPASEAEI QPQTQVTLRC HIDGHPRPTY QWFRDGTPLS DGQSNHTVSS 
KERNLTLRPA GPEHSGLYSC CAHSAFGQAC SSQNFTLSIA DESFARVVLA PQDVVVARYE 
EAMFHCQFSA QPPPSLQWLF EDETPITNRS RPPHLRRATV FANGSLLLTQ VRPRNAGIYR 
CIGQGQRGPP IILEATLHLA EIEDMPLFEP RVFTAGSEER VTCLPPKGLP EPSVWWEHAG 
VRLPTHGRVY QKGHELVLAN IAESDAGVYT CHAANLAGQR RQDVNITVAT VPSWLKKPQD 
SQLEEGKPGY LDCLTQATPK PTVVWYRNQM LISEDSRFEV FKNGTLRINS VEVYDGTWYR 
CMSSTPAGSI EAQARVQVLE KLKFTPPPQP QQCMEFDKEA TVPCSATGRE KPTIKWERAD 
GSSLPEWVTD NAGTLHFARV TRDDAGNYTC IASNGPQGQI RAHVQLTVAV FITFKVEPER 
TTVYQGHTAL LQCEAQGDPK PLIQWKGKDR ILDPTKLGPR MHIFQNGSLV IHDVAPEDSG 
RYTCIAGNSC NIKHTEAPLY VVDKPVPEES EGPGSPPPYK MIQTIGLSVG AAVAYIIAVL 
GLMFYCKKRC KAKRLQKQPE GEEPEMECLN GGPLQNGQPS AEIQEEVALT SLGSGPAATN 
KRHSTSDKMH FPRSSLQPIT TLGKSEFGEV FLAKAQGLEE GVAETLVLVK SLQSKDEQQQ 
LDFRRELEMF GKLNHANVVR LLGLCREAEP HYMVLEYVDL GDLKQFLRIS KSKDEKLKSQ 
PLSTKQKVAL CTQVALGMEH LSNNRFVHKD LAARNCLVSA QRQVKVSALG LSKDVYNSEY 
YHFRQAWVPL RWMSPEAILE GDFSTKSDVW AFGVLMWEVF THGEMPHGGQ ADDEVLADLQ 
AGKARLPQPE GCPSKLYRLM QRCWALSPKD RPSFSEIASA LGDSTVDSKP