Details for: CELA3B

Gene ID: 23436

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: CELA3B

Ensembl ID: ENSG00000219073

Description: chymotrypsin like elastase 3B

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • pancreatic acinar cell CL0002064
    CSI 5.95
    rCSI 7.91%
    PRS 99.75
  • pancreatic ductal cell CL0002079
    CSI 2.79
    rCSI 5.43%
    PRS 99.9

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

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  • Node Size: Proportional to Target Cell CSI magnitude
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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [CELA3B](/details-gene/23436), or Chymotrypsin-like elastase family member 3B, is a protein-coding gene located on chromosome 1. It encodes a serine-type endopeptidase, a class of enzymes crucial for proteolysis. Functional annotation confirms its role in peptidase activity ([GO:0008233](https://www.ebi.ac.uk/QuickGO/term/GO:0008233)) and specifically serine-type endopeptidase activity ([GO:0004252](https://www.ebi.ac.uk/QuickGO/term/GO:0004252)), primarily occurring in the [extracellular space](/details-go/GO0005615) ([GO:0005615](https://www.ebi.ac.uk/QuickGO/term/GO:0005615)). Expression data reveals its highly specific role within the exocrine pancreas, where it serves as a defining marker for [pancreatic acinar cell](/details-cell/CL0002064)s. Its discovery was part of the characterization of a novel class of human pancreatic elastase isozymes ([Link](https://doi.org/10.1016/s0021-9258(19)57291-x)). ## Cellular Roles and Expression Landscape The expression profile of [CELA3B](/details-gene/23436) demonstrates a remarkably high degree of tissue specificity. **Overall**, its significance is almost exclusively confined to the pancreas. It is most significantly expressed in [pancreatic acinar cell](/details-cell/CL0002064), with a Cell Significance Index (CSI) of 5.95, underscoring its role as a primary product of these specialized secretory cells. [Pancreatic acinar cell](/details-cell/CL0002064)s are responsible for synthesizing and secreting large quantities of digestive enzymes, and the high expression of [CELA3B](/details-gene/23436) is consistent with its function as a key digestive protease. The gene also shows notable, albeit lower, significance in [pancreatic ductal cell](/details-cell/CL0002079) (CSI: 2.79), which are involved in transporting these enzymes. The absence of significant expression in other cell types suggests a highly specialized biological function centered on protein digestion within the gastrointestinal tract. ## Pathways and Molecular Function The molecular function of [CELA3B](/details-gene/23436) is clearly defined by its associated Gene Ontology terms. As a secreted enzyme, it operates in the [extracellular space](/details-go/GO0005615) ([GO:0005615](https://www.ebi.ac.uk/QuickGO/term/GO:0005615)), which in its primary physiological context is the lumen of the small intestine following secretion from the pancreas. Its core function is Proteolysis ([GO:0006508](https://www.ebi.ac.uk/QuickGO/term/GO:0006508)), achieved through its Serine-type endopeptidase activity ([GO:0004252](https://www.ebi.ac.uk/QuickGO/term/GO:0004252)). Like other digestive proteases, it is synthesized as an inactive zymogen (proproteinase E) and becomes activated after secretion, preventing autodigestion of the pancreatic tissue ([Link](https://doi.org/10.1016/0006-291x(89)91104-2)). This enzymatic activity is essential for breaking down dietary proteins into smaller peptides for absorption. ## Research Directions The highly specific expression and potent proteolytic function of [CELA3B](/details-gene/23436) make its dysregulation a critical area for investigation, particularly in pancreatic diseases. While its physiological role is well-established, its potential contribution to pathology warrants further exploration. Based on its function, we can propose the following testable hypotheses: 1. **Premature intracellular activation of [CELA3B](/details-gene/23436) within [pancreatic acinar cell](/details-cell/CL0002064)s is a contributing factor to the cellular injury and inflammation observed in acute pancreatitis.** The mis-localization or premature cleavage of its zymogen form could initiate a proteolytic cascade, leading to pancreatic autodigestion. 2. **Aberrant expression or secretion of [CELA3B](/details-gene/23436) by pancreatic cancer cells contributes to tumor progression.** In the context of pancreatic ductal adenocarcinoma (PDAC), the enzyme may facilitate invasion and metastasis by degrading components of the extracellular matrix, a mechanism observed for other proteases in cancer. To investigate the second hypothesis, a key experiment would be to **characterize [CELA3B](/details-gene/23436) expression and function in the tumor microenvironment of PDAC.** This could be achieved by applying spatial transcriptomics and proteomics to human PDAC tissue samples to determine if cancer cells, particularly at the invasive front, upregulate [CELA3B](/details-gene/23436). Functional validation could involve using CRISPR-Cas9 to knock out [CELA3B](/details-gene/23436) in PDAC cell lines and assessing changes in their invasive capacity using 3D organoid models or in vitro Matrigel invasion assays. Given that [CELA3B](/details-gene/23436) is a secreted enzyme, it represents a potentially druggable target. If it is shown to be a key driver of tissue damage in pancreatitis or tumor invasion in PDAC, **inhibition** would be the therapeutic strategy. The development of specific small-molecule or antibody-based inhibitors that target the active site of [CELA3B](/details-gene/23436) could offer a targeted approach to mitigate its pathological activity while potentially sparing other related proteases, thereby minimizing off-target effects.

Genular Protein ID: 886599566

Symbol: CEL3B_HUMAN

Name: Chymotrypsin-like elastase family member 3B

UniProtKB Accession Codes:

P08861 B2RE44 P11423 Q5VU28 Q5VU29 Q5VU30

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 DMDM 1 DNASU 1 DisGeNET 1 EC 2 EMBL 6 Ensembl 1 ExpressionAtlas 1 GO 4 Gene3D 1 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyConnect 1 GlyCosmos 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 7 KEGG 1 MANE-Select 1 MEROPS 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PIR 1 PRINTS 1 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 RNAct 1 RefSeq 1 SMART 1 SMR 1 STRING 1 SUPFAM 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 2826474

Title: Identification of a novel class of elastase isozyme, human pancreatic elastase III, by cDNA and genomic gene cloning.

PubMed ID: 2826474

DOI: 10.1016/s0021-9258(19)57291-x

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 16710414

Title: The DNA sequence and biological annotation of human chromosome 1.

PubMed ID: 16710414

DOI: 10.1038/nature04727

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 3477287

Title: Primary structure of human pancreatic protease E determined by sequence analysis of the cloned mRNA.

PubMed ID: 3477287

DOI: 10.1021/bi00386a030

PubMed ID: 2675835

Title: Generation of a subunit III-like protein by autolysis of human and porcine proproteinase E in a binary complex with procarboxypeptidase A.

PubMed ID: 2675835

DOI: 10.1016/0006-291x(89)91104-2

PubMed ID: 3178837

Title: Characterization of two glycoproteins of human pancreatic juice: P35, a truncated protease E and P19, precursor of protein X.

PubMed ID: 3178837

DOI: 10.1016/s0006-291x(88)80842-8

PubMed ID: 2753124

Title: Identification of a procarboxypeptidase A-truncated protease E binary complex in human pancreatic juice.

PubMed ID: 2753124

DOI: 10.1016/0014-5793(89)80712-4

PubMed ID: 2737288

Title: Localization and characterization of the glycosylation site of human pancreatic elastase 1.

PubMed ID: 2737288

DOI: 10.1016/0014-5793(89)80640-4

PubMed ID: 10620133

Title: Human elastase 1: evidence for expression in the skin and the identification of a frequent frameshift polymorphism.

PubMed ID: 10620133

DOI: 10.1046/j.1523-1747.2000.00825.x

Sequence Information:

  • Length: 270
  • Mass: 29263
  • Checksum: F738C5F8F5195D8C
  • Sequence:
    • MMLRLLSSLL LVAVASGYGP PSSRPSSRVV NGEDAVPYSW PWQVSLQYEK SGSFYHTCGG 
SLIAPDWVVT AGHCISSSRT YQVVLGEYDR AVKEGPEQVI PINSGDLFVH PLWNRSCVAC 
GNDIALIKLS RSAQLGDAVQ LASLPPAGDI LPNETPCYIT GWGRLYTNGP LPDKLQEALL 
PVVDYEHCSR WNWWGSSVKK TMVCAGGDIR SGCNGDSGGP LNCPTEDGGW QVHGVTSFVS 
AFGCNTRRKP TVFTRVSAFI DWIEETIASH