NIT2 | ENSG00000114021 | NCBI 56954

Details for: NIT2

Gene ID: 56954

Gene Type: Protein-coding - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: NIT2

Ensembl ID: ENSG00000114021

Description: nitrilase family member 2

Cell Significance Landscape

Display Count:

Associated with

Immune system
(R-HSA-168256)
Innate immune system
(R-HSA-168249)
Neutrophil degranulation
(R-HSA-6798695)
2-oxoglutaramate amidase activity
(GO:0106008)
Asparagine metabolic process
(GO:0006528)
Centrosome
(GO:0005813)
Cytosol
(GO:0005829)
Extracellular exosome
(GO:0070062)
Extracellular region
(GO:0005576)
Glutamine metabolic process
(GO:0006541)
Mitochondrion
(GO:0005739)
Omega-amidase activity
(GO:0050152)
Oxaloacetate metabolic process
(GO:0006107)
Specific granule lumen
(GO:0035580)
Tertiary granule lumen
(GO:1904724)

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

mature B cell CL0000785

CSI 13.76

rCSI 11.96%

PRS 72.6
intestinal epithelial cell CL0002563

CSI 11.21

rCSI 11.72%

PRS 59.91
peripheral nervous system neuron CL2000032

CSI 7.53

rCSI 10.26%

PRS 53.69
plasmacytoid dendritic cell, human CL0001058

CSI 7.46

rCSI 5.21%

PRS 64.66
fallopian tube secretory epithelial cell CL4030006

CSI 6.46

rCSI 6.22%

PRS 61.91
double negative thymocyte CL0002489

CSI 5.39

rCSI 3.75%

PRS 73.02
epithelial cell of lung CL0000082

CSI 5.37

rCSI 4.45%

PRS 61.1
epithelial cell of proximal tubule CL0002306

CSI 4.75

rCSI 11.59%

PRS 55.43
alternatively activated macrophage CL0000890

CSI 4.7

rCSI 5.91%

PRS 73.65
immature B cell CL0000816

CSI 4.41

rCSI 3.28%

PRS 75.19
acinar cell CL0000622

CSI 4.23

rCSI 6.2%

PRS 73.45
lung macrophage CL1001603

CSI 3.94

rCSI 8.8%

PRS 69.66
hematopoietic multipotent progenitor cell CL0000837

CSI 3.76

rCSI 9.06%

PRS 79.49
neuroblast (sensu Vertebrata) CL0000031

CSI 3.65

rCSI 4.69%

PRS 59.02
pancreatic ductal cell CL0002079

CSI 3.45

rCSI 6.71%

PRS 64.86
regular ventricular cardiac myocyte CL0002131

CSI 3.43

rCSI 21.42%

PRS 53.76
common dendritic progenitor CL0001029

CSI 3.38

rCSI 4.24%

PRS 72.28
nasal mucosa goblet cell CL0002480

CSI 3.25

rCSI 3.77%

PRS 69.37
naive T cell CL0000898

CSI 3.08

rCSI 2.14%

PRS 77.07
midzonal region hepatocyte CL0019028

CSI 3.01

rCSI 7.07%

PRS 66.64
unswitched memory B cell CL0000970

CSI 2.9

rCSI 2.44%

PRS 78.79
perivascular cell CL4033054

CSI 2.87

rCSI 3.92%

PRS 67.86
retinal cone cell CL0000573

CSI 2.71

rCSI 4.36%

PRS 51.4
epithelial cell CL0000066

CSI 2.71

rCSI 4.16%

PRS 57.71
mesenchymal cell CL0008019

CSI 2.68

rCSI 6.81%

PRS 56.09
ionocyte CL0005006

CSI 2.66

rCSI 2.85%

PRS 61.26
fibroblast of lung CL0002553

CSI 2.6

rCSI 2.42%

PRS 62.44
erythroid progenitor cell CL0000038

CSI 2.56

rCSI 14.65%

PRS 71.08
effector CD8-positive, alpha-beta T cell CL0001050

CSI 2.47

rCSI 1.88%

PRS 74.87
enteroendocrine cell CL0000164

CSI 2.46

rCSI 3.37%

PRS 63.51
CD4-positive, CD25-positive, alpha-beta regulatory T cell CL0000792

CSI 2.45

rCSI 2.41%

PRS 77.49
secretory cell CL0000151

CSI 2.45

rCSI 2.56%

PRS 62.14
kidney epithelial cell CL0002518

CSI 2.33

rCSI 4.45%

PRS 81.45
intestine goblet cell CL0019031

CSI 2.31

rCSI 2.05%

PRS 59.83
renal alpha-intercalated cell CL0005011

CSI 2.29

rCSI 3.07%

PRS 70.75
enteric smooth muscle cell CL0002504

CSI 2.21

rCSI 3.16%

PRS 64.12
CD4-positive helper T cell CL0000492

CSI 2.2

rCSI 1.67%

PRS 75.58
epithelial cell of lower respiratory tract CL0002632

CSI 2.16

rCSI 1.67%

PRS 63.76
interstitial cell of Cajal CL0002088

CSI 2.11

rCSI 2.68%

PRS 68.33
plasmablast CL0000980

CSI 2.08

rCSI 1.64%

PRS 68.56
elicited macrophage CL0000861

CSI 2.06

rCSI 1.89%

PRS 70.75
respiratory basal cell CL0002633

CSI 2.04

rCSI 2.12%

PRS 67.53
early lymphoid progenitor CL0000936

CSI 2.04

rCSI 1.79%

PRS 67.41
placental villous trophoblast CL2000060

CSI 2.01

rCSI 3.11%

PRS 60.03
interneuron CL0000099

CSI 2.01

rCSI 4.03%

PRS 50.92
kidney loop of Henle thin descending limb epithelial cell CL1001111

CSI 2

rCSI 2.84%

PRS 58.18
colon epithelial cell CL0011108

CSI 1.99

rCSI 2.09%

PRS 58.27
megakaryocyte-erythroid progenitor cell CL0000050

CSI 1.98

rCSI 1.79%

PRS 58.73
myoepithelial cell CL0000185

CSI 1.95

rCSI 4.95%

PRS 69.88
mesodermal cell CL0000222

CSI 1.95

rCSI 2.34%

PRS 59.93
intrahepatic cholangiocyte CL0002538

CSI 1.95

rCSI 4.67%

PRS 72.22
promonocyte CL0000559

CSI 1.93

rCSI 3.31%

PRS 70.71
respiratory hillock cell CL4030023

CSI 1.92

rCSI 3.42%

PRS 75.23
club cell CL0000158

CSI 1.9

rCSI 2.78%

PRS 57.85
granulocyte monocyte progenitor cell CL0000557

CSI 1.85

rCSI 1.6%

PRS 66.84
skin fibroblast CL0002620

CSI 1.84

rCSI 1.58%

PRS 67.21
pancreatic A cell CL0000171

CSI 1.82

rCSI 1.9%

PRS 65.48
common myeloid progenitor CL0000049

CSI 1.82

rCSI 1.47%

PRS 63.29
neural crest cell CL0011012

CSI 1.77

rCSI 1.4%

PRS 48.69
hematopoietic precursor cell CL0008001

CSI 1.77

rCSI 1.82%

PRS 78.14
pro-B cell CL0000826

CSI 1.77

rCSI 1.46%

PRS 64.07
myofibroblast cell CL0000186

CSI 1.75

rCSI 2.42%

PRS 62.93
centrilobular region hepatocyte CL0019029

CSI 1.74

rCSI 4.53%

PRS 65.52
mucus secreting cell CL0000319

CSI 1.73

rCSI 2.75%

PRS 72.92
transit amplifying cell of colon CL0009011

CSI 1.71

rCSI 2%

PRS 64.2
stem cell CL0000034

CSI 1.69

rCSI 1.63%

PRS 52.47
pancreatic D cell CL0000173

CSI 1.68

rCSI 1.65%

PRS 64.49
luminal epithelial cell of mammary gland CL0002326

CSI 1.67

rCSI 3.04%

PRS 76.99
duct epithelial cell CL0000068

CSI 1.66

rCSI 2.43%

PRS 66.5
lung pericyte CL0009089

CSI 1.65

rCSI 4.37%

PRS 70.71
ciliated cell CL0000064

CSI 1.65

rCSI 2.67%

PRS 58.45
dendritic cell, human CL0001056

CSI 1.64

rCSI 2.52%

PRS 70.94
Cajal-Retzius cell CL0000695

CSI 1.64

rCSI 12.82%

PRS 73.32
pulmonary ionocyte CL0017000

CSI 1.63

rCSI 1.99%

PRS 69.32
extravillous trophoblast CL0008036

CSI 1.59

rCSI 1.97%

PRS 58.43
goblet cell CL0000160

CSI 1.58

rCSI 1.49%

PRS 61.43
group 3 innate lymphoid cell CL0001071

CSI 1.57

rCSI 1.18%

PRS 67.33
lung neuroendocrine cell CL1000223

CSI 1.53

rCSI 2.27%

PRS 67.24
myeloid leukocyte CL0000766

CSI 1.53

rCSI 1.41%

PRS 63.28
pvalb GABAergic cortical interneuron CL4023018

CSI 1.49

rCSI 1.86%

PRS 41.71
epithelial cell of proximal tubule segment 3 CL4030011

CSI 1.48

rCSI 11.79%

PRS 70.93
hematopoietic stem cell CL0000037

CSI 1.48

rCSI 0.98%

PRS 64.99
keratinocyte CL0000312

CSI 1.45

rCSI 1.22%

PRS 66.17
mucous neck cell CL0000651

CSI 1.45

rCSI 2.09%

PRS 72.56
conjunctival epithelial cell CL1000432

CSI 1.43

rCSI 2.19%

PRS 62.54
ciliated epithelial cell CL0000067

CSI 1.43

rCSI 1.26%

PRS 49.69
hepatocyte CL0000182

CSI 1.35

rCSI 2.42%

PRS 60.98
radial glial cell CL0000681

CSI 1.33

rCSI 1.85%

PRS 60.43
lung ciliated cell CL1000271

CSI 1.33

rCSI 1.54%

PRS 51.92
pancreatic acinar cell CL0002064

CSI 1.33

rCSI 1.77%

PRS 68.32
Langerhans cell CL0000453

CSI 1.28

rCSI 1.96%

PRS 77.41
lung secretory cell CL1000272

CSI 1.27

rCSI 3.14%

PRS 60.26
differentiation-committed oligodendrocyte precursor CL4023059

CSI 1.22

rCSI 2.23%

PRS 53.79
promyelocyte CL0000836

CSI 1.21

rCSI 1.75%

PRS 71.38
multi-ciliated epithelial cell CL0005012

CSI 1.15

rCSI 1.15%

PRS 55.1
Hofbauer cell CL3000001

CSI 1.13

rCSI 2.13%

PRS 72.35
bronchus fibroblast of lung CL2000093

CSI 1.12

rCSI 0.91%

PRS 62.32
alveolar macrophage CL0000583

CSI 1.1

rCSI 1.82%

PRS 67.07
effector memory CD8-positive, alpha-beta T cell, terminally differentiated CL0001062

CSI 1.1

rCSI 5.52%

PRS 74.37
glioblast CL0000030

CSI 1.09

rCSI 1.74%

PRS 54.42

pre-conventional dendritic cell CL0002010

CSI 0.2

rCSI 2.4%

PRS 87.7%
indirect pathway medium spiny neuron CL4023029

CSI 0.2

rCSI 5.0%

PRS 43.5%
direct pathway medium spiny neuron CL4023026

CSI 0.3

rCSI 6.1%

PRS 42.7%
intestinal crypt stem cell of colon CL0009043

CSI 0.3

rCSI 2.1%

PRS 77.9%
podocyte CL0000653

CSI 0.4

rCSI 1.6%

PRS 61.4%
L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041

CSI 0.4

rCSI 1.6%

PRS 42.0%
L6b glutamatergic cortical neuron CL4023038

CSI 0.5

rCSI 1.4%

PRS 45.3%
pancreatic stellate cell CL0002410

CSI 0.5

rCSI 2.6%

PRS 70.8%
microcirculation associated smooth muscle cell CL0008035

CSI 0.5

rCSI 1.4%

PRS 63.2%
type B pancreatic cell CL0000169

CSI 0.5

rCSI 1.1%

PRS 59.8%
mesenchymal stem cell CL0000134

CSI 0.7

rCSI 7.3%

PRS 74.2%
intermediate monocyte CL0002393

CSI 0.7

rCSI 1.0%

PRS 66.1%
amacrine cell CL0000561

CSI 0.7

rCSI 2.0%

PRS 51.6%
ciliated columnar cell of tracheobronchial tree CL0002145

CSI 0.7

rCSI 1.7%

PRS 58.2%
myeloid lineage restricted progenitor cell CL0000839

CSI 0.7

rCSI 3.8%

PRS 83.3%
L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040

CSI 0.8

rCSI 2.0%

PRS 42.3%
mammary gland epithelial cell CL0002327

CSI 0.8

rCSI 2.9%

PRS 74.3%
vascular associated smooth muscle cell CL0000359

CSI 0.9

rCSI 2.8%

PRS 62.2%
myeloid dendritic cell CL0000782

CSI 0.9

rCSI 1.3%

PRS 77.9%
caudal ganglionic eminence derived cortical interneuron CL4023064

CSI 1.0

rCSI 1.8%

PRS 42.7%
periportal region hepatocyte CL0019026

CSI 1.0

rCSI 4.1%

PRS 66.7%
enterocyte CL0000584

CSI 1.0

rCSI 1.7%

PRS 65.6%
glioblast CL0000030

CSI 1.1

rCSI 1.7%

PRS 54.4%
effector memory CD8-positive, alpha-beta T cell, terminally differentiated CL0001062

CSI 1.1

rCSI 5.5%

PRS 74.4%
alveolar macrophage CL0000583

CSI 1.1

rCSI 1.8%

PRS 67.1%
bronchus fibroblast of lung CL2000093

CSI 1.1

rCSI 0.9%

PRS 62.3%
Hofbauer cell CL3000001

CSI 1.1

rCSI 2.1%

PRS 72.4%
multi-ciliated epithelial cell CL0005012

CSI 1.2

rCSI 1.2%

PRS 55.1%
promyelocyte CL0000836

CSI 1.2

rCSI 1.8%

PRS 71.4%
differentiation-committed oligodendrocyte precursor CL4023059

CSI 1.2

rCSI 2.2%

PRS 53.8%
lung secretory cell CL1000272

CSI 1.3

rCSI 3.1%

PRS 60.3%
Langerhans cell CL0000453

CSI 1.3

rCSI 2.0%

PRS 77.4%
pancreatic acinar cell CL0002064

CSI 1.3

rCSI 1.8%

PRS 68.3%
lung ciliated cell CL1000271

CSI 1.3

rCSI 1.5%

PRS 51.9%
radial glial cell CL0000681

CSI 1.3

rCSI 1.9%

PRS 60.4%
hepatocyte CL0000182

CSI 1.4

rCSI 2.4%

PRS 61.0%
ciliated epithelial cell CL0000067

CSI 1.4

rCSI 1.3%

PRS 49.7%
conjunctival epithelial cell CL1000432

CSI 1.4

rCSI 2.2%

PRS 62.5%
mucous neck cell CL0000651

CSI 1.5

rCSI 2.1%

PRS 72.6%
keratinocyte CL0000312

CSI 1.5

rCSI 1.2%

PRS 66.2%
hematopoietic stem cell CL0000037

CSI 1.5

rCSI 1.0%

PRS 65.0%
epithelial cell of proximal tubule segment 3 CL4030011

CSI 1.5

rCSI 11.8%

PRS 70.9%
pvalb GABAergic cortical interneuron CL4023018

CSI 1.5

rCSI 1.9%

PRS 41.7%
myeloid leukocyte CL0000766

CSI 1.5

rCSI 1.4%

PRS 63.3%
lung neuroendocrine cell CL1000223

CSI 1.5

rCSI 2.3%

PRS 67.2%
group 3 innate lymphoid cell CL0001071

CSI 1.6

rCSI 1.2%

PRS 67.3%
goblet cell CL0000160

CSI 1.6

rCSI 1.5%

PRS 61.4%
extravillous trophoblast CL0008036

CSI 1.6

rCSI 2.0%

PRS 58.4%
pulmonary ionocyte CL0017000

CSI 1.6

rCSI 2.0%

PRS 69.3%
Cajal-Retzius cell CL0000695

CSI 1.6

rCSI 12.8%

PRS 73.3%
dendritic cell, human CL0001056

CSI 1.6

rCSI 2.5%

PRS 70.9%
ciliated cell CL0000064

CSI 1.7

rCSI 2.7%

PRS 58.5%
lung pericyte CL0009089

CSI 1.7

rCSI 4.4%

PRS 70.7%
duct epithelial cell CL0000068

CSI 1.7

rCSI 2.4%

PRS 66.5%
luminal epithelial cell of mammary gland CL0002326

CSI 1.7

rCSI 3.0%

PRS 77.0%
pancreatic D cell CL0000173

CSI 1.7

rCSI 1.7%

PRS 64.5%
stem cell CL0000034

CSI 1.7

rCSI 1.6%

PRS 52.5%
transit amplifying cell of colon CL0009011

CSI 1.7

rCSI 2.0%

PRS 64.2%
mucus secreting cell CL0000319

CSI 1.7

rCSI 2.8%

PRS 72.9%
centrilobular region hepatocyte CL0019029

CSI 1.7

rCSI 4.5%

PRS 65.5%
myofibroblast cell CL0000186

CSI 1.8

rCSI 2.4%

PRS 62.9%
pro-B cell CL0000826

CSI 1.8

rCSI 1.5%

PRS 64.1%
hematopoietic precursor cell CL0008001

CSI 1.8

rCSI 1.8%

PRS 78.1%
neural crest cell CL0011012

CSI 1.8

rCSI 1.4%

PRS 48.7%
common myeloid progenitor CL0000049

CSI 1.8

rCSI 1.5%

PRS 63.3%
pancreatic A cell CL0000171

CSI 1.8

rCSI 1.9%

PRS 65.5%
skin fibroblast CL0002620

CSI 1.8

rCSI 1.6%

PRS 67.2%
granulocyte monocyte progenitor cell CL0000557

CSI 1.9

rCSI 1.6%

PRS 66.8%
club cell CL0000158

CSI 1.9

rCSI 2.8%

PRS 57.9%
respiratory hillock cell CL4030023

CSI 1.9

rCSI 3.4%

PRS 75.2%
promonocyte CL0000559

CSI 1.9

rCSI 3.3%

PRS 70.7%
intrahepatic cholangiocyte CL0002538

CSI 2.0

rCSI 4.7%

PRS 72.2%
mesodermal cell CL0000222

CSI 2.0

rCSI 2.3%

PRS 59.9%
myoepithelial cell CL0000185

CSI 2.0

rCSI 5.0%

PRS 69.9%
megakaryocyte-erythroid progenitor cell CL0000050

CSI 2.0

rCSI 1.8%

PRS 58.7%
colon epithelial cell CL0011108

CSI 2.0

rCSI 2.1%

PRS 58.3%
kidney loop of Henle thin descending limb epithelial cell CL1001111

CSI 2.0

rCSI 2.8%

PRS 58.2%
interneuron CL0000099

CSI 2.0

rCSI 4.0%

PRS 50.9%
placental villous trophoblast CL2000060

CSI 2.0

rCSI 3.1%

PRS 60.0%
early lymphoid progenitor CL0000936

CSI 2.0

rCSI 1.8%

PRS 67.4%
respiratory basal cell CL0002633

CSI 2.0

rCSI 2.1%

PRS 67.5%
elicited macrophage CL0000861

CSI 2.1

rCSI 1.9%

PRS 70.8%
plasmablast CL0000980

CSI 2.1

rCSI 1.6%

PRS 68.6%
interstitial cell of Cajal CL0002088

CSI 2.1

rCSI 2.7%

PRS 68.3%
epithelial cell of lower respiratory tract CL0002632

CSI 2.2

rCSI 1.7%

PRS 63.8%
CD4-positive helper T cell CL0000492

CSI 2.2

rCSI 1.7%

PRS 75.6%
enteric smooth muscle cell CL0002504

CSI 2.2

rCSI 3.2%

PRS 64.1%
renal alpha-intercalated cell CL0005011

CSI 2.3

rCSI 3.1%

PRS 70.8%
intestine goblet cell CL0019031

CSI 2.3

rCSI 2.1%

PRS 59.8%
kidney epithelial cell CL0002518

CSI 2.3

rCSI 4.5%

PRS 81.5%
secretory cell CL0000151

CSI 2.5

rCSI 2.6%

PRS 62.1%
CD4-positive, CD25-positive, alpha-beta regulatory T cell CL0000792

CSI 2.5

rCSI 2.4%

PRS 77.5%
enteroendocrine cell CL0000164

CSI 2.5

rCSI 3.4%

PRS 63.5%
effector CD8-positive, alpha-beta T cell CL0001050

CSI 2.5

rCSI 1.9%

PRS 74.9%
erythroid progenitor cell CL0000038

CSI 2.6

rCSI 14.7%

PRS 71.1%
fibroblast of lung CL0002553

CSI 2.6

rCSI 2.4%

PRS 62.4%
ionocyte CL0005006

CSI 2.7

rCSI 2.9%

PRS 61.3%
mesenchymal cell CL0008019

CSI 2.7

rCSI 6.8%

PRS 56.1%
epithelial cell CL0000066

CSI 2.7

rCSI 4.2%

PRS 57.7%
retinal cone cell CL0000573

CSI 2.7

rCSI 4.4%

PRS 51.4%

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Interaction Source:

Pathway Categories (for ONTOLOGY source):

Baseline Cell Type:

Baseline Cell Context(s): Comma-separated if multiple.

Target Cell Type:

Target Cell Context(s): Comma-separated if multiple.

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Node Color (Target Cell CSI, relative to current network):
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- High
- Medium
- Low
- Very Low
- CSI N/A
Node Size: Proportional to Target Cell CSI magnitude
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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

Description
Proteins

## Summary [NIT2](/details-gene/56954), or Nitrilase Family Member 2, is a protein-coding gene located on chromosome 3q12.2. It encodes an omega-amidase enzyme that plays a key role in amino acid metabolism, specifically the catabolism of asparagine and glutamine ([Link](https://doi.org/10.1016/j.biochi.2009.07.003)). The **Overall** expression profile reveals its significance in a diverse array of cell types, including high levels in immune cells like [mature B cells](/details-cell/CL0000785) and [plasmacytoid dendritic cells](/details-cell/CL0001058), as well as in various epithelial tissues such as [intestinal epithelial cells](/details-cell/CL0002563). This broad expression pattern suggests a fundamental metabolic function essential for multiple cellular lineages. Research has implicated [NIT2](/details-gene/56954) as a putative tumor suppressor and noted its dysregulation in developmental disorders like Down syndrome ([Link](https://doi.org/10.1111/j.1742-4658.2007.05828.x); [Link](https://pubmed.ncbi.nlm.nih.gov/14528910/)). ## Cellular Roles and Expression Landscape The **Overall** expression data for [NIT2](/details-gene/56954) highlights its importance across multiple, functionally distinct biological systems. It shows the highest significance in [mature B cells](/details-cell/CL0000785) (CSI: 13.76), indicating a prominent role in the adaptive immune system. Its significance extends to other immune lineages, including [plasmacytoid dendritic cells, human](/details-cell/CL0001058), [alternatively activated macrophages](/details-cell/CL0000890), and B cell precursors like [immature B cells](/details-cell/CL0000816) and [hematopoietic multipotent progenitor cells](/details-cell/CL0000837). Concurrently, [NIT2](/details-gene/56954) demonstrates substantial significance in non-hematopoietic cells, particularly in epithelial and secretory tissues. It is a key gene in [intestinal epithelial cells](/details-cell/CL0002563) (CSI: 11.21) and is also highly significant in [fallopian tube secretory epithelial cells](/details-cell/CL4030006), [epithelial cells of the lung](/details-cell/CL0000082), and [epithelial cells of the proximal tubule](/details-cell/CL0002306). Furthermore, its expression in the [peripheral nervous system neuron](/details-cell/CL2000032) suggests its metabolic functions are also required in the nervous system. This widespread yet high-significance expression pattern is consistent with a housekeeping-like enzymatic role in cellular metabolism rather than a function as a specific lineage marker. ## Pathways and Molecular Function [NIT2](/details-gene/56954)'s primary molecular function is as an enzyme with [omega-amidase activity](/details-go/GO:0050152), specifically [2-oxoglutaramate amidase activity](/details-go/GO:0106008). This enzymatic activity directly supports its involvement in biological processes such as the [asparagine metabolic process](/details-go/GO:0006528) and the [glutamine metabolic process](/details-go/GO:0006541), as confirmed by biochemical studies ([Link](https://doi.org/10.1016/j.biochi.2009.07.003); [Link](https://doi.org/10.1074/jbc.m111.259119)). Cellular component annotations indicate that [NIT2](/details-gene/56954) is localized to several compartments, including the [cytosol](/details-go/GO:0005829), [mitochondrion](/details-go/GO:0005739), and [extracellular exosomes](/details-go/GO:0070062), suggesting it may have diverse roles depending on its subcellular location. Notably, its presence in the [specific granule lumen](/details-go/GO:0035580) and [tertiary granule lumen](/details-go/GO:1904724) aligns with its annotation in the Reactome pathway for [Neutrophil degranulation](/details-pathway/R-HSA-6798695). This strongly suggests a role for [NIT2](/details-gene/56954) within the [innate immune system](/details-pathway/R-HSA-168249), where it may be released during neutrophil activation to modulate the local metabolic microenvironment. ## Research Directions Although the available data is from an **Overall** context, publications suggest that [NIT2](/details-gene/56954) function is highly relevant to pathology. It has been identified as a putative tumor suppressor, with evidence of allelic imbalance in various cancers ([Link](https://doi.org/10.1111/j.1742-4658.2007.05828.x)). Its dysregulation in the fetal Down syndrome brain further points to a critical role in development and disease ([Link](https://pubmed.ncbi.nlm.nih.gov/14528910/)). Based on its known functions and expression profile, several testable hypotheses can be proposed: 1. Given its localization to neutrophil granules and role in the [Neutrophil degranulation](/details-pathway/R-HSA-6798695) pathway, [NIT2](/details-gene/56954) may be secreted during inflammation to deplete local asparagine and glutamine, thereby modulating the metabolic activity and function of other nearby immune or target cells. 2. The tumor suppressor function of [NIT2](/details-gene/56954) may be directly linked to its metabolic activity. Loss of [NIT2](/details-gene/56954) function could lead to an accumulation of its substrates (e.g., 2-oxoglutaramate), which may have downstream oncogenic signaling effects or contribute to a metabolic rewiring that supports cell proliferation. To test the second hypothesis, a compelling experimental approach would be: - **Experiment:** Perform CRISPR-Cas9-mediated knockout of [NIT2](/details-gene/56954) in cancer cell lines that show allelic imbalance for this gene. The metabolic impact could be precisely quantified using stable isotope tracing with 13C-labeled glutamine followed by mass spectrometry-based metabolomics to measure flux alterations. These metabolic profiles should be correlated with functional assays assessing cell proliferation, colony formation in soft agar, and apoptosis to directly link the enzymatic activity of [NIT2](/details-gene/56954) to cancer-associated phenotypes. From a therapeutic standpoint, [NIT2](/details-gene/56954) represents a challenging but potentially valuable target. As a putative tumor suppressor, the therapeutic strategy would involve **activation** or functional restoration, not inhibition. This could be explored through small-molecule activators or gene therapy approaches aimed at re-introducing its enzymatic activity in tumor cells. However, its broad expression across many healthy tissues raises a significant risk of off-target effects, necessitating highly targeted delivery systems for any potential therapeutic agent.

Disclaimer: This in-silico analysis is generated by an AI language model and may contain inaccuracies or hallucinations. However, it is cross-referenced with curated gene expression data from major biological sources. Please verify the information before use.

Omega-amidase NIT2

Genular Protein ID: 174947742

Symbol: NIT2_HUMAN

Name: Omega-amidase NIT2

UniProtKB Accession Codes:

Q9NQR4 B2R9A3 D3DN47 Q8WUF0

Database IDs:

Citations:

PubMed ID: 10959838

Title: Crystal structure of the worm NitFhit Rosetta stone protein reveals a Nit tetramer binding two Fhit dimers.

PubMed ID: 10959838

DOI: 10.1016/s0960-9822(00)00621-7

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 14528910

Title: Deranged hypothetical proteins Rik protein, Nit protein 2 and mitochondrial inner membrane protein, Mitofilin, in fetal Down syndrome brain.

PubMed ID: 14528910

PubMed ID: 15592455

Title: Immunoaffinity profiling of tyrosine phosphorylation in cancer cells.

PubMed ID: 15592455

DOI: 10.1038/nbt1046

PubMed ID: 17488281

Title: Growth inhibitory effect of the human NIT2 gene and its allelic imbalance in cancers.

PubMed ID: 17488281

DOI: 10.1111/j.1742-4658.2007.05828.x

PubMed ID: 19595734

Title: Identification of the putative tumor suppressor Nit2 as omega-amidase, an enzyme metabolically linked to glutamine and asparagine transamination.

PubMed ID: 19595734

DOI: 10.1016/j.biochi.2009.07.003

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 22674578

Title: Structural insights into the catalytic active site and activity of human Nit2/omega-amidase: kinetic assay and molecular dynamics simulation.

PubMed ID: 22674578

DOI: 10.1074/jbc.m111.259119

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 25944712

Title: N-terminome analysis of the human mitochondrial proteome.

PubMed ID: 25944712

DOI: 10.1002/pmic.201400617

Sequence Information:

Length: 276
Mass: 30608
Checksum: 32FA797601A419C6
Sequence:

MTSFRLALIQ LQISSIKSDN VTRACSFIRE AATQGAKIVS LPECFNSPYG AKYFPEYAEK 
IPGESTQKLS EVAKECSIYL IGGSIPEEDA GKLYNTCAVF GPDGTLLAKY RKIHLFDIDV 
PGKITFQESK TLSPGDSFST FDTPYCRVGL GICYDMRFAE LAQIYAQRGC QLLVYPGAFN 
LTTGPAHWEL LQRSRAVDNQ VYVATASPAR DDKASYVAWG HSTVVNPWGE VLAKAGTEEA 
IVYSDIDLKK LAEIRQQIPV FRQKRSDLYA VEMKKP

Details for: NIT2

Cell Significance Landscape

Associated with

Significant Cells

Network Configuration

Legend:

Other Information

Crystal structure of the worm NitFhit Rosetta stone protein reveals a Nit tetramer binding two Fhit dimers. PubMed ID: 10959838

Complete sequencing and characterization of 21,243 full-length human cDNAs. PubMed ID: 14702039

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). PubMed ID: 15489334

Deranged hypothetical proteins Rik protein, Nit protein 2 and mitochondrial inner membrane protein, Mitofilin, in fetal Down syndrome brain. PubMed ID: 14528910

Immunoaffinity profiling of tyrosine phosphorylation in cancer cells. PubMed ID: 15592455

Growth inhibitory effect of the human NIT2 gene and its allelic imbalance in cancers. PubMed ID: 17488281

Identification of the putative tumor suppressor Nit2 as omega-amidase, an enzyme metabolically linked to glutamine and asparagine transamination. PubMed ID: 19595734

Initial characterization of the human central proteome. PubMed ID: 21269460

Structural insights into the catalytic active site and activity of human Nit2/omega-amidase: kinetic assay and molecular dynamics simulation. PubMed ID: 22674578

An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. PubMed ID: 24275569

N-terminome analysis of the human mitochondrial proteome. PubMed ID: 25944712