LIMD2 | ENSG00000136490 | NCBI 80774

Details for: LIMD2

Gene ID: 80774

Gene Type: Protein-coding - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: LIMD2

Ensembl ID: ENSG00000136490

Description: LIM domain containing 2

Cell Significance Landscape

Display Count:

Associated with

Actin cytoskeleton
(GO:0015629)
Actin filament binding
(GO:0051015)
Actin filament bundle assembly
(GO:0051017)
Cytosol
(GO:0005829)
Metal ion binding
(GO:0046872)
Nucleoplasm
(GO:0005654)
Plasma membrane
(GO:0005886)
Protein binding
(GO:0005515)

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

small pre-B-II cell CL0000954

CSI 76.33

rCSI 73.41%

PRS 97.68
mature B cell CL0000785

CSI 72.88

rCSI 63.36%

PRS 97.7
CD16-positive, CD56-dim natural killer cell, human CL0000939

CSI 69.65

rCSI 46.42%

PRS 97.32
CD14-low, CD16-positive monocyte CL0002396

CSI 67.05

rCSI 51.66%

PRS 96.24
immature B cell CL0000816

CSI 66.82

rCSI 49.65%

PRS 97.58
naive B cell CL0000788

CSI 65.98

rCSI 56.59%

PRS 96.28
CD16-negative, CD56-bright natural killer cell, human CL0000938

CSI 64

rCSI 47.99%

PRS 98.25
naive thymus-derived CD8-positive, alpha-beta T cell CL0000900

CSI 62.57

rCSI 43.95%

PRS 97.67
class switched memory B cell CL0000972

CSI 53.06

rCSI 39.61%

PRS 97.64
central memory CD4-positive, alpha-beta T cell CL0000904

CSI 52.37

rCSI 30.93%

PRS 99.33
central memory CD8-positive, alpha-beta T cell CL0000907

CSI 52.36

rCSI 35.28%

PRS 98.88
plasmablast CL0000980

CSI 50.7

rCSI 39.88%

PRS 95.14
memory B cell CL0000787

CSI 50.56

rCSI 49.93%

PRS 96.4
megakaryocyte-erythroid progenitor cell CL0000050

CSI 47.02

rCSI 42.46%

PRS 92.96
double-positive, alpha-beta thymocyte CL0000809

CSI 44.64

rCSI 45.5%

PRS 97.16
hematopoietic stem cell CL0000037

CSI 41.56

rCSI 27.62%

PRS 94.84
naive thymus-derived CD4-positive, alpha-beta T cell CL0000895

CSI 40.57

rCSI 50.99%

PRS 97.71
myeloid leukocyte CL0000766

CSI 34.06

rCSI 31.43%

PRS 94.97
effector CD8-positive, alpha-beta T cell CL0001050

CSI 33.57

rCSI 25.53%

PRS 98.81
CD4-positive, alpha-beta memory T cell CL0000897

CSI 33.12

rCSI 23.77%

PRS 98.76
myofibroblast cell CL0000186

CSI 33.07

rCSI 45.8%

PRS 91.33
conventional dendritic cell CL0000990

CSI 32.24

rCSI 26.92%

PRS 88.26
mucosal invariant T cell CL0000940

CSI 32.01

rCSI 25.86%

PRS 97.09
common dendritic progenitor CL0001029

CSI 31.49

rCSI 39.52%

PRS 96.89
alpha-beta T cell CL0000789

CSI 31.05

rCSI 36.38%

PRS 98.21
early lymphoid progenitor CL0000936

CSI 28.85

rCSI 25.34%

PRS 96.15
group 3 innate lymphoid cell CL0001071

CSI 28.55

rCSI 21.45%

PRS 95.76
unswitched memory B cell CL0000970

CSI 27.33

rCSI 23%

PRS 98.21
naive T cell CL0000898

CSI 26.81

rCSI 18.66%

PRS 98.82
activated CD4-positive, alpha-beta T cell CL0000896

CSI 26.04

rCSI 24.07%

PRS 98.48
activated CD8-positive, alpha-beta T cell CL0000906

CSI 25.62

rCSI 25.18%

PRS 97.57
common lymphoid progenitor CL0000051

CSI 25.26

rCSI 33.76%

PRS 98.45
CD4-positive helper T cell CL0000492

CSI 24.84

rCSI 18.79%

PRS 98.54
activated type II NK T cell CL0000931

CSI 24.8

rCSI 27.92%

PRS 98.21
non-classical monocyte CL0000875

CSI 24.74

rCSI 39.65%

PRS 96.97
promonocyte CL0000559

CSI 24.5

rCSI 41.98%

PRS 95.05
activated CD8-positive, alpha-beta T cell, human CL0001049

CSI 24.27

rCSI 41.51%

PRS 97.53
enteroendocrine cell CL0000164

CSI 24.18

rCSI 33.04%

PRS 92.08
mature T cell CL0002419

CSI 23.95

rCSI 18.63%

PRS 98.29
CD8-positive, CD28-negative, alpha-beta regulatory T cell CL0000920

CSI 23.44

rCSI 46.73%

PRS 97.65
classical monocyte CL0000860

CSI 23.25

rCSI 34.47%

PRS 96.52
precursor B cell CL0000817

CSI 22.98

rCSI 20.13%

PRS 96.38
mononuclear phagocyte CL0000113

CSI 22.66

rCSI 49.88%

PRS 95.83
B cell CL0000236

CSI 22.64

rCSI 30.29%

PRS 92.76
mature NK T cell CL0000814

CSI 22.54

rCSI 28.83%

PRS 96.3
gamma-delta T cell CL0000798

CSI 22.47

rCSI 26.39%

PRS 96.46
effector memory CD4-positive, alpha-beta T cell CL0000905

CSI 22.21

rCSI 30.25%

PRS 98.75
granulocyte monocyte progenitor cell CL0000557

CSI 21.83

rCSI 18.9%

PRS 95.17
hematopoietic precursor cell CL0008001

CSI 21.44

rCSI 22.06%

PRS 97.21
CD4-positive, alpha-beta T cell CL0000624

CSI 21.4

rCSI 27.38%

PRS 94.83
late pro-B cell CL0002048

CSI 21.32

rCSI 53.42%

PRS 97.77
regulatory T cell CL0000815

CSI 21.04

rCSI 24.4%

PRS 87.96
plasmacytoid dendritic cell, human CL0001058

CSI 20.7

rCSI 14.45%

PRS 96.15
double negative thymocyte CL0002489

CSI 20.51

rCSI 14.26%

PRS 98.43
fraction A pre-pro B cell CL0002045

CSI 20.24

rCSI 23.17%

PRS 96.1
common myeloid progenitor CL0000049

CSI 19.94

rCSI 16.12%

PRS 94.71
germinal center B cell CL0000844

CSI 19.45

rCSI 58%

PRS 95.72
CD4-positive, alpha-beta thymocyte CL0000810

CSI 19.38

rCSI 15.52%

PRS 95.71
CD14-positive monocyte CL0001054

CSI 19.37

rCSI 24.13%

PRS 97.69
CD8-positive, alpha-beta memory T cell, CD45RO-positive CL0001203

CSI 19.34

rCSI 23.43%

PRS 76.53
CD8-positive, alpha-beta memory T cell CL0000909

CSI 19.08

rCSI 19.93%

PRS 97.7
CD8-positive, alpha-beta cytotoxic T cell CL0000794

CSI 18.92

rCSI 22.59%

PRS 98.44
large pre-B-II cell CL0000957

CSI 18.66

rCSI 53.28%

PRS 93.14
transitional stage B cell CL0000818

CSI 18.38

rCSI 60.16%

PRS 98.16
neural crest cell CL0011012

CSI 18.25

rCSI 14.43%

PRS 88.83
lung macrophage CL1001603

CSI 18.21

rCSI 40.68%

PRS 97.12
effector memory CD8-positive, alpha-beta T cell CL0000913

CSI 17.94

rCSI 16.34%

PRS 98.45
T follicular helper cell CL0002038

CSI 17.18

rCSI 12.86%

PRS 98.48
myeloid cell CL0000763

CSI 16.63

rCSI 68.48%

PRS 95.17
Kupffer cell CL0000091

CSI 16.51

rCSI 37.75%

PRS 94.74
natural killer cell CL0000623

CSI 16.38

rCSI 31.75%

PRS 94.1
granulocyte CL0000094

CSI 16.36

rCSI 25%

PRS 96.56
promyelocyte CL0000836

CSI 16.28

rCSI 23.48%

PRS 95.2
pro-B cell CL0000826

CSI 16.21

rCSI 13.42%

PRS 94.65
macrophage CL0000235

CSI 15.74

rCSI 28.63%

PRS 93.79
T cell CL0000084

CSI 15.71

rCSI 30.72%

PRS 96.69
plasmacytoid dendritic cell CL0000784

CSI 15.22

rCSI 15.42%

PRS 97
effector CD4-positive, alpha-beta T cell CL0001044

CSI 14.44

rCSI 41.41%

PRS 99.04
CD1c-positive myeloid dendritic cell CL0002399

CSI 14.32

rCSI 17.3%

PRS 97.09
follicular B cell CL0000843

CSI 14.31

rCSI 52.03%

PRS 97.56
platelet CL0000233

CSI 13.78

rCSI 57.18%

PRS 90.23
CD14-positive, CD16-positive monocyte CL0002397

CSI 13.72

rCSI 17.98%

PRS 97.89
erythrocyte CL0000232

CSI 13.57

rCSI 30.78%

PRS 91.85
IgA plasma cell CL0000987

CSI 13.25

rCSI 13.56%

PRS 94.29
CD8-positive, alpha-beta thymocyte CL0000811

CSI 13.09

rCSI 20.41%

PRS 97.26
dendritic cell CL0000451

CSI 12.95

rCSI 15.95%

PRS 93.07
CD4-positive, CD25-positive, alpha-beta regulatory T cell CL0000792

CSI 12.45

rCSI 12.22%

PRS 98.62
peripheral nervous system neuron CL2000032

CSI 12.36

rCSI 16.84%

PRS 88.72
inflammatory macrophage CL0000863

CSI 11.79

rCSI 20.15%

PRS 98.76
monocyte CL0000576

CSI 11.71

rCSI 21.16%

PRS 95.01
CD4-positive, alpha-beta cytotoxic T cell CL0000934

CSI 11.6

rCSI 15.93%

PRS 98.82
elicited macrophage CL0000861

CSI 11.51

rCSI 10.56%

PRS 97.05
progenitor cell CL0011026

CSI 11.48

rCSI 24.41%

PRS 88.36
glutamatergic neuron CL0000679

CSI 11.05

rCSI 22.7%

PRS 84.33
CD8-alpha-alpha-positive, alpha-beta intraepithelial T cell CL0000915

CSI 11.03

rCSI 50.12%

PRS 98.42
cerebral cortex GABAergic interneuron CL0010011

CSI 10.67

rCSI 31.51%

PRS 93.48
mesenchymal cell CL0008019

CSI 10.67

rCSI 27.1%

PRS 90.15
T-helper 17 cell CL0000899

CSI 10.48

rCSI 8.32%

PRS 98.85
pulmonary artery endothelial cell CL1001568

CSI 10.37

rCSI 14.11%

PRS 96.7
alternatively activated macrophage CL0000890

CSI 10.09

rCSI 12.69%

PRS 97.55

L6b glutamatergic cortical neuron CL4023038

CSI 0.6

rCSI 2.0%

PRS 85.4%
immature alpha-beta T cell CL0000790

CSI 0.8

rCSI 12.0%

PRS 98.3%
NKp44-negative group 3 innate lymphoid cell, human CL0001080

CSI 1.2

rCSI 37.6%

PRS 98.0%
pre-conventional dendritic cell CL0002010

CSI 1.3

rCSI 16.9%

PRS 98.2%
pro-T cell CL0000827

CSI 1.5

rCSI 35.6%

PRS 99.1%
Cajal-Retzius cell CL0000695

CSI 1.6

rCSI 12.9%

PRS 94.4%
cytotoxic T cell CL0000910

CSI 1.7

rCSI 9.8%

PRS 91.8%
myelocyte CL0002193

CSI 1.8

rCSI 11.6%

PRS 97.3%
near-projecting glutamatergic cortical neuron CL4023012

CSI 2.0

rCSI 7.7%

PRS 84.6%
group 3 innate lymphoid cell, human CL0001078

CSI 2.1

rCSI 44.2%

PRS 96.5%
L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041

CSI 2.3

rCSI 8.4%

PRS 82.6%
erythroid progenitor cell CL0000038

CSI 2.3

rCSI 13.4%

PRS 94.8%
megakaryocyte progenitor cell CL0000553

CSI 2.3

rCSI 42.9%

PRS 98.6%
metallothionein-positive alveolar macrophage CL4033042

CSI 2.4

rCSI 26.1%

PRS 97.5%
B-1 B cell CL0000819

CSI 2.5

rCSI 65.4%

PRS 98.0%
smooth muscle cell CL0000192

CSI 2.6

rCSI 6.1%

PRS 88.9%
intestinal tuft cell CL0019032

CSI 2.6

rCSI 4.0%

PRS 94.8%
double negative T regulatory cell CL0011024

CSI 2.8

rCSI 53.1%

PRS 98.2%
IgM plasma cell CL0000986

CSI 2.8

rCSI 12.7%

PRS 97.8%
B-2 B cell CL0000822

CSI 3.0

rCSI 63.5%

PRS 97.8%
caudal ganglionic eminence derived cortical interneuron CL4023064

CSI 3.1

rCSI 5.4%

PRS 83.8%
antibody secreting cell CL0000946

CSI 3.2

rCSI 14.2%

PRS 99.0%
myeloid dendritic cell, human CL0001057

CSI 3.5

rCSI 19.4%

PRS 98.4%
mature alpha-beta T cell CL0000791

CSI 3.5

rCSI 12.7%

PRS 99.1%
myeloid dendritic cell CL0000782

CSI 3.7

rCSI 5.3%

PRS 98.5%
radial glial cell CL0000681

CSI 3.8

rCSI 5.2%

PRS 92.5%
hepatic pit cell CL2000054

CSI 3.9

rCSI 52.9%

PRS 96.1%
corticothalamic-projecting glutamatergic cortical neuron CL4023013

CSI 4.2

rCSI 25.0%

PRS 84.8%
CD34-positive, CD56-positive, CD117-positive common innate lymphoid precursor, human CL0001074

CSI 4.5

rCSI 52.8%

PRS 97.6%
lymphoid lineage restricted progenitor cell CL0000838

CSI 4.8

rCSI 18.6%

PRS 99.0%
helper T cell CL0000912

CSI 4.9

rCSI 7.0%

PRS 89.5%
microcirculation associated smooth muscle cell CL0008035

CSI 5.0

rCSI 14.5%

PRS 93.2%
basophil mast progenitor cell CL0002028

CSI 5.1

rCSI 27.0%

PRS 98.4%
effector memory CD8-positive, alpha-beta T cell, terminally differentiated CL0001062

CSI 5.1

rCSI 25.6%

PRS 98.2%
pancreatic stellate cell CL0002410

CSI 5.2

rCSI 30.5%

PRS 94.9%
thymocyte CL0000893

CSI 5.5

rCSI 19.6%

PRS 98.9%
mesodermal cell CL0000222

CSI 5.5

rCSI 6.6%

PRS 93.1%
lung resident memory CD8-positive, alpha-beta T cell CL4033039

CSI 5.6

rCSI 14.4%

PRS 98.3%
IgG plasma cell CL0000985

CSI 5.6

rCSI 6.7%

PRS 95.9%
myeloid lineage restricted progenitor cell CL0000839

CSI 5.9

rCSI 30.7%

PRS 98.4%
T-helper 1 cell CL0000545

CSI 6.0

rCSI 10.8%

PRS 98.2%
cerebral cortex endothelial cell CL1001602

CSI 6.2

rCSI 10.7%

PRS 90.1%
stromal cell CL0000499

CSI 6.5

rCSI 18.1%

PRS 90.8%
innate lymphoid cell CL0001065

CSI 6.9

rCSI 14.2%

PRS 88.3%
activated CD4-positive, alpha-beta T cell, human CL0001043

CSI 7.1

rCSI 17.1%

PRS 98.5%
CD14-positive, CD16-negative classical monocyte CL0002057

CSI 7.2

rCSI 43.6%

PRS 97.5%
hematopoietic multipotent progenitor cell CL0000837

CSI 7.5

rCSI 18.0%

PRS 98.0%
neuroblast (sensu Vertebrata) CL0000031

CSI 7.6

rCSI 9.7%

PRS 90.7%
intermediate monocyte CL0002393

CSI 7.6

rCSI 11.4%

PRS 96.8%
megakaryocyte CL0000556

CSI 7.7

rCSI 33.6%

PRS 94.0%
intraepithelial lymphocyte CL0002496

CSI 8.0

rCSI 21.7%

PRS 98.2%
eosinophil CL0000771

CSI 8.2

rCSI 53.9%

PRS 98.0%
L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040

CSI 8.4

rCSI 20.3%

PRS 82.3%
natural T-regulatory cell CL0000903

CSI 8.4

rCSI 15.9%

PRS 98.9%
professional antigen presenting cell CL0000145

CSI 8.7

rCSI 30.0%

PRS 95.7%
basophil CL0000767

CSI 8.9

rCSI 18.8%

PRS 96.8%
Langerhans cell CL0000453

CSI 9.0

rCSI 13.7%

PRS 97.8%
erythroblast CL0000765

CSI 9.1

rCSI 24.1%

PRS 93.9%
neutrophil CL0000775

CSI 9.1

rCSI 50.8%

PRS 91.5%
dendritic cell, human CL0001056

CSI 9.3

rCSI 14.4%

PRS 97.5%
memory T cell CL0000813

CSI 9.4

rCSI 18.2%

PRS 98.6%
group 2 innate lymphoid cell CL0001069

CSI 10.1

rCSI 54.6%

PRS 98.4%
alternatively activated macrophage CL0000890

CSI 10.1

rCSI 12.7%

PRS 97.6%
pulmonary artery endothelial cell CL1001568

CSI 10.4

rCSI 14.1%

PRS 96.7%
T-helper 17 cell CL0000899

CSI 10.5

rCSI 8.3%

PRS 98.9%
mesenchymal cell CL0008019

CSI 10.7

rCSI 27.1%

PRS 90.2%
cerebral cortex GABAergic interneuron CL0010011

CSI 10.7

rCSI 31.5%

PRS 93.5%
CD8-alpha-alpha-positive, alpha-beta intraepithelial T cell CL0000915

CSI 11.0

rCSI 50.1%

PRS 98.4%
glutamatergic neuron CL0000679

CSI 11.1

rCSI 22.7%

PRS 84.3%
progenitor cell CL0011026

CSI 11.5

rCSI 24.4%

PRS 88.4%
elicited macrophage CL0000861

CSI 11.5

rCSI 10.6%

PRS 97.1%
CD4-positive, alpha-beta cytotoxic T cell CL0000934

CSI 11.6

rCSI 15.9%

PRS 98.8%
monocyte CL0000576

CSI 11.7

rCSI 21.2%

PRS 95.0%
inflammatory macrophage CL0000863

CSI 11.8

rCSI 20.2%

PRS 98.8%
peripheral nervous system neuron CL2000032

CSI 12.4

rCSI 16.8%

PRS 88.7%
CD4-positive, CD25-positive, alpha-beta regulatory T cell CL0000792

CSI 12.5

rCSI 12.2%

PRS 98.6%
dendritic cell CL0000451

CSI 13.0

rCSI 16.0%

PRS 93.1%
CD8-positive, alpha-beta thymocyte CL0000811

CSI 13.1

rCSI 20.4%

PRS 97.3%
IgA plasma cell CL0000987

CSI 13.3

rCSI 13.6%

PRS 94.3%
erythrocyte CL0000232

CSI 13.6

rCSI 30.8%

PRS 91.9%
CD14-positive, CD16-positive monocyte CL0002397

CSI 13.7

rCSI 18.0%

PRS 97.9%
platelet CL0000233

CSI 13.8

rCSI 57.2%

PRS 90.2%
follicular B cell CL0000843

CSI 14.3

rCSI 52.0%

PRS 97.6%
CD1c-positive myeloid dendritic cell CL0002399

CSI 14.3

rCSI 17.3%

PRS 97.1%
effector CD4-positive, alpha-beta T cell CL0001044

CSI 14.4

rCSI 41.4%

PRS 99.0%
plasmacytoid dendritic cell CL0000784

CSI 15.2

rCSI 15.4%

PRS 97.0%
T cell CL0000084

CSI 15.7

rCSI 30.7%

PRS 96.7%
macrophage CL0000235

CSI 15.7

rCSI 28.6%

PRS 93.8%
pro-B cell CL0000826

CSI 16.2

rCSI 13.4%

PRS 94.7%
promyelocyte CL0000836

CSI 16.3

rCSI 23.5%

PRS 95.2%
granulocyte CL0000094

CSI 16.4

rCSI 25.0%

PRS 96.6%
natural killer cell CL0000623

CSI 16.4

rCSI 31.8%

PRS 94.1%
Kupffer cell CL0000091

CSI 16.5

rCSI 37.8%

PRS 94.7%
myeloid cell CL0000763

CSI 16.6

rCSI 68.5%

PRS 95.2%
T follicular helper cell CL0002038

CSI 17.2

rCSI 12.9%

PRS 98.5%
effector memory CD8-positive, alpha-beta T cell CL0000913

CSI 17.9

rCSI 16.3%

PRS 98.5%
lung macrophage CL1001603

CSI 18.2

rCSI 40.7%

PRS 97.1%
neural crest cell CL0011012

CSI 18.3

rCSI 14.4%

PRS 88.8%
transitional stage B cell CL0000818

CSI 18.4

rCSI 60.2%

PRS 98.2%
large pre-B-II cell CL0000957

CSI 18.7

rCSI 53.3%

PRS 93.1%

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Interaction Source:

Pathway Categories (for ONTOLOGY source):

Baseline Cell Type:

Baseline Cell Context(s): Comma-separated if multiple.

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Target Cell Context(s): Comma-separated if multiple.

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Node Color (Target Cell CSI, relative to current network):
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Node Size: Proportional to Target Cell CSI magnitude
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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

Description
Proteins

## Summary [LIMD2](/details-gene/80774) (LIM domain containing 2) is a protein-coding gene located on chromosome 17q23.3. It encodes a small, LIM-only protein that functions as an adaptor or scaffolding molecule involved in cytoskeletal organization. Functionally, it is associated with actin filament binding and assembly, localizing to the actin cytoskeleton, cytosol, and plasma membrane. Expression data indicates that [LIMD2](/details-gene/80774) is a highly significant gene within the hematopoietic system, showing prominent expression across various lymphocyte populations including B cells, T cells, and natural killer (NK) cells. Research has implicated [LIMD2](/details-gene/80774) in the regulation of cell motility and tumor progression, where it has been shown to be overexpressed in metastatic lesions and to activate integrin-linked kinase (ILK) [Link](https://doi.org/10.1158/0008-5472.can-13-1275). ## Cellular Roles and Expression Landscape **Overall**, the expression profile of [LIMD2](/details-gene/80774) demonstrates its integral role in the immune system, with particularly high significance in cells of the lymphoid lineage. The gene is most prominently expressed in the B cell compartment, with high significance scores across multiple developmental stages. This includes early progenitors like [small pre-B-II cell](/details-cell/CL0000954) (CSI: 76.33), as well as [immature B cell](/details-cell/CL0000816), [naive B cell](/details-cell/CL0000788), and [mature B cell](/details-cell/CL0000785). Its sustained high expression in activated and memory populations, such as [class switched memory B cell](/details-cell/CL0000972) and [plasmablast](/details-cell/CL0000980), suggests a continuous role from development through to terminal differentiation and effector function. Beyond the B cell lineage, [LIMD2](/details-gene/80774) is also a key marker for cytotoxic lymphocytes. It shows high significance in both [CD16-positive, CD56-dim natural killer cell, human](/details-cell/CL0000939) (CSI: 69.65) and [CD16-negative, CD56-bright natural killer cell, human](/details-cell/CL0000938), indicating a broad function in NK cell biology. Similarly, its expression in [naive thymus-derived CD8-positive, alpha-beta T cell](/details-cell/CL0000900) and central memory T cells ([CD4-positive](/details-cell/CL0000904) and [CD8-positive](/details-cell/CL0000907)) points towards a role in T cell function. The gene's significance in [CD14-low, CD16-positive monocyte](/details-cell/CL0002396) further extends its relevance to the myeloid compartment, albeit more specifically to a pro-inflammatory subset. ## Pathways and Molecular Function The functional annotations for [LIMD2](/details-gene/80774) are highly consistent with its expression pattern in motile, immunologically active cells. Its primary molecular functions are related to the cytoskeleton, including 'Actin filament binding' ([GO:0051015](https://www.ebi.ac.uk/QuickGO/term/GO:0051015)) and participation in 'Actin filament bundle assembly' ([GO:0051017](https://www.ebi.ac.uk/QuickGO/term/GO:0051017)). This role is supported by its localization to the 'Actin cytoskeleton' ([GO:0015629](https://www.ebi.ac.uk/QuickGO/term/GO:0015629)) and 'Plasma membrane' ([GO:0005886](https://www.ebi.ac.uk/QuickGO/term/GO:0005886)). These functions are critical for the dynamic processes that define lymphocytes, such as migration, immune synapse formation, and phagocytosis. The reported interaction with and activation of integrin-linked kinase (ILK) provides a direct mechanistic link between [LIMD2](/details-gene/80774) and the regulation of cell adhesion and motility, processes central to immune surveillance and cancer metastasis [Link](https://doi.org/10.1158/0008-5472.can-13-1275). Its ability to bind metal ions ([GO:0046872](https://www.ebi.ac.uk/QuickGO/term/GO:0046872)) is characteristic of its LIM domain structure, which is often involved in mediating protein-protein interactions. ## Research Directions The widespread and high-level expression of [LIMD2](/details-gene/80774) in lymphocytes, coupled with its established role in promoting cell motility in cancer, opens several avenues for future investigation into its physiological and pathological functions. ### Proposed Hypotheses: 1. **[LIMD2](/details-gene/80774) is a key regulator of B cell receptor (BCR) signaling and antigen presentation.** Given its high expression throughout B cell development and its role in actin dynamics, [LIMD2](/details-gene/80774) may be essential for the cytoskeletal reorganization required for BCR clustering, signaling initiation, and the subsequent formation of the B cell-T cell immune synapse. 2. **The function of [LIMD2](/details-gene/80774) is critical for the cytotoxic effector function of NK cells and CD8+ T cells.** Its involvement in actin regulation may be required for the formation of a stable lytic synapse, polarization of the microtubule-organizing center, and the directed release of cytotoxic granules toward a target cell. 3. **In hematological malignancies like B-cell lymphomas, upregulation of [LIMD2](/details-gene/80774) may contribute to disease progression.** Drawing from its described role in solid tumors [Link](https://doi.org/10.1158/0008-5472.can-13-1275), elevated [LIMD2](/details-gene/80774) expression in malignant B cells could enhance their migratory capacity, tissue infiltration, and resistance to apoptosis by augmenting ILK-mediated signaling. ### Key Experimental Approach: To test the hypothesis that [LIMD2](/details-gene/80774) is critical for NK cell cytotoxic function (Hypothesis 2), a targeted knockout of the gene could be generated in a human NK cell line (e.g., NK-92) using a CRISPR-Cas9 system. The cytotoxic capacity of [LIMD2](/details-gene/80774)-deficient NK cells would be compared to wild-type controls using a standard chromium-release assay or flow cytometry-based killing assay with a susceptible target cell line (e.g., K562). Furthermore, high-resolution live-cell imaging could be used to directly visualize and quantify lytic synapse formation, F-actin accumulation at the synapse, and lytic granule convergence in knockout versus control cells upon contact with targets. ### Therapeutic Potential: Given its documented role in promoting tumor progression and metastasis, [LIMD2](/details-gene/80774) represents a potential therapeutic target for **inhibition**. As an intracellular scaffolding protein, directly targeting it may be challenging. However, developing small molecule inhibitors that disrupt its specific protein-protein interaction with integrin-linked kinase (ILK) could be a viable strategy to attenuate its pro-migratory effects in cancer. A significant challenge for this approach is the gene's high expression in a wide range of essential immune cells. Systemic inhibition could lead to immunosuppressive side effects. Therefore, therapeutic strategies might require cancer-specific delivery systems or could be most relevant for hematological malignancies where the tumor cells themselves could be targeted.

Disclaimer: This in-silico analysis is generated by an AI language model and may contain inaccuracies or hallucinations. However, it is cross-referenced with curated gene expression data from major biological sources. Please verify the information before use.

LIMD2_HUMAN

Genular Protein ID: 289835598

Symbol: LIMD2_HUMAN

Name: N/A

UniProtKB Accession Codes:

Q9BT23 D3DU16 Q96S91

Database IDs:

Citations:

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 19413330

Title: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.

PubMed ID: 19413330

DOI: 10.1021/ac9004309

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 24590809

Title: LIMD2 is a small LIM-only protein overexpressed in metastatic lesions that regulates cell motility and tumor progression by directly binding to and activating the integrin-linked kinase.

PubMed ID: 24590809

DOI: 10.1158/0008-5472.can-13-1275

Sequence Information:

Length: 127
Mass: 14070
Checksum: B63174FCF0486956
Sequence:

MFQAAGAAQA TPSHDAKGGG SSTVQRSKSF SLRAQVKETC AACQKTVYPM ERLVADKLIF 
HNSCFCCKHC HTKLSLGSYA ALHGEFYCKP HFQQLFKSKG NYDEGFGRKQ HKELWAHKEV 
DPGTKTA