Details for: NDUFAF3

Gene ID: 25915

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: NDUFAF3

Ensembl ID: ENSG00000178057

Description: NADH:ubiquinone oxidoreductase complex assembly factor 3

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • ciliated epithelial cell CL0000067
    CSI 75.08
    rCSI 66.02%
    PRS 13.79
  • multi-ciliated epithelial cell CL0005012
    CSI 74.09
    rCSI 73.94%
    PRS 16.18
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 53.48
    rCSI 48.3%
    PRS 17.04
  • pancreatic A cell CL0000171
    CSI 35.46
    rCSI 37.14%
    PRS 20.22
  • fallopian tube secretory epithelial cell CL4030006
    CSI 32.36
    rCSI 31.15%
    PRS 19.62
  • plasmablast CL0000980
    CSI 30.72
    rCSI 24.17%
    PRS 22.88
  • pancreatic D cell CL0000173
    CSI 30.32
    rCSI 29.82%
    PRS 20.51
  • lung ciliated cell CL1000271
    CSI 30.06
    rCSI 34.76%
    PRS 13.94
  • cardiac muscle cell CL0000746
    CSI 30.02
    rCSI 43.08%
    PRS 14.67
  • duct epithelial cell CL0000068
    CSI 29.6
    rCSI 43.31%
    PRS 20.09
  • fraction A pre-pro B cell CL0002045
    CSI 28.41
    rCSI 32.52%
    PRS 38.26
  • granulocyte monocyte progenitor cell CL0000557
    CSI 25.75
    rCSI 22.3%
    PRS 21.32
  • common myeloid progenitor CL0000049
    CSI 24.87
    rCSI 20.11%
    PRS 18.97
  • deuterosomal cell CL4033044
    CSI 24.57
    rCSI 83.06%
    PRS 30.31
  • ciliated columnar cell of tracheobronchial tree CL0002145
    CSI 21.95
    rCSI 50.04%
    PRS 19.57
  • intestinal epithelial cell CL0002563
    CSI 21.92
    rCSI 22.91%
    PRS 19.8
  • large pre-B-II cell CL0000957
    CSI 21.42
    rCSI 61.16%
    PRS 32.23
  • common dendritic progenitor CL0001029
    CSI 18.45
    rCSI 23.15%
    PRS 24.55
  • promyelocyte CL0000836
    CSI 18.36
    rCSI 26.48%
    PRS 26.53
  • promonocyte CL0000559
    CSI 18.12
    rCSI 31.04%
    PRS 25.73
  • microcirculation associated smooth muscle cell CL0008035
    CSI 17.29
    rCSI 50.05%
    PRS 21.43
  • stem cell CL0000034
    CSI 16.9
    rCSI 16.29%
    PRS 13.66
  • retinal ganglion cell CL0000740
    CSI 15.91
    rCSI 35.14%
    PRS 13.59
  • interstitial cell of Cajal CL0002088
    CSI 14
    rCSI 17.82%
    PRS 22.02
  • colon epithelial cell CL0011108
    CSI 13.91
    rCSI 14.57%
    PRS 17.7
  • hematopoietic stem cell CL0000037
    CSI 13.75
    rCSI 9.14%
    PRS 22.75
  • primitive red blood cell CL0002355
    CSI 13.25
    rCSI 71.49%
    PRS 33.24
  • peripheral nervous system neuron CL2000032
    CSI 13
    rCSI 17.72%
    PRS 16.82
  • pancreatic epsilon cell CL0005019
    CSI 12.45
    rCSI 58.02%
    PRS 42.03
  • type B pancreatic cell CL0000169
    CSI 12.06
    rCSI 26.69%
    PRS 17.54
  • retinal pigment epithelial cell CL0002586
    CSI 11.63
    rCSI 23.09%
    PRS 19.96
  • epithelial cell of lower respiratory tract CL0002632
    CSI 11.51
    rCSI 8.92%
    PRS 18.19
  • double negative thymocyte CL0002489
    CSI 11.37
    rCSI 7.9%
    PRS 22.65
  • kidney epithelial cell CL0002518
    CSI 11.27
    rCSI 21.51%
    PRS 41.54
  • pancreatic PP cell CL0002275
    CSI 11.21
    rCSI 44.62%
    PRS 32.46
  • pro-B cell CL0000826
    CSI 11.07
    rCSI 9.16%
    PRS 19.23
  • megakaryocyte CL0000556
    CSI 11.05
    rCSI 47.94%
    PRS 32.97
  • pancreatic stellate cell CL0002410
    CSI 10.52
    rCSI 61.24%
    PRS 28.16
  • perivascular cell CL4033054
    CSI 10.33
    rCSI 14.13%
    PRS 21.47
  • ionocyte CL0005006
    CSI 10.31
    rCSI 11.05%
    PRS 17.48
  • neural progenitor cell CL0011020
    CSI 10.2
    rCSI 44.89%
    PRS 17.92
  • retina horizontal cell CL0000745
    CSI 9.74
    rCSI 14.85%
    PRS 17.62
  • activated CD4-positive, alpha-beta T cell CL0000896
    CSI 9.72
    rCSI 8.99%
    PRS 34.07
  • pancreatic ductal cell CL0002079
    CSI 8.71
    rCSI 16.93%
    PRS 19.41
  • effector memory CD8-positive, alpha-beta T cell CL0000913
    CSI 8.7
    rCSI 7.93%
    PRS 28.93
  • activated type II NK T cell CL0000931
    CSI 8.38
    rCSI 9.43%
    PRS 30.24
  • bronchus fibroblast of lung CL2000093
    CSI 8.29
    rCSI 6.73%
    PRS 20.04
  • epithelial cell of proximal tubule CL0002306
    CSI 8.24
    rCSI 20.14%
    PRS 18.64
  • naive T cell CL0000898
    CSI 8.24
    rCSI 5.73%
    PRS 26.9
  • myeloid lineage restricted progenitor cell CL0000839
    CSI 8.23
    rCSI 42.48%
    PRS 36.77
  • tracheobronchial smooth muscle cell CL0019019
    CSI 8.2
    rCSI 14.46%
    PRS 24.94
  • mesenchymal stem cell CL0000134
    CSI 7.91
    rCSI 86.63%
    PRS 33.66
  • dendritic cell CL0000451
    CSI 7.9
    rCSI 9.74%
    PRS 53.19
  • T follicular helper cell CL0002038
    CSI 7.72
    rCSI 5.78%
    PRS 30.14
  • transit amplifying cell of colon CL0009011
    CSI 7.66
    rCSI 8.99%
    PRS 21.97
  • goblet cell CL0000160
    CSI 7.64
    rCSI 7.22%
    PRS 19.89
  • enteric smooth muscle cell CL0002504
    CSI 7.3
    rCSI 10.42%
    PRS 21.33
  • pancreatic acinar cell CL0002064
    CSI 7.29
    rCSI 9.69%
    PRS 20.79
  • mature NK T cell CL0000814
    CSI 7.26
    rCSI 9.29%
    PRS 63.19
  • IgA plasma cell CL0000987
    CSI 7.12
    rCSI 7.29%
    PRS 34.89
  • epithelial cell CL0000066
    CSI 7.01
    rCSI 10.77%
    PRS 26.06
  • myeloid leukocyte CL0000766
    CSI 6.9
    rCSI 6.37%
    PRS 19.62
  • secretory cell CL0000151
    CSI 6.83
    rCSI 7.13%
    PRS 19.44
  • CD8-positive, alpha-beta thymocyte CL0000811
    CSI 6.72
    rCSI 10.48%
    PRS 43.42
  • pulmonary artery endothelial cell CL1001568
    CSI 6.56
    rCSI 8.93%
    PRS 28.65
  • pulmonary alveolar type 2 cell CL0002063
    CSI 6.43
    rCSI 9.97%
    PRS 28.33
  • P/D1 enteroendocrine cell CL0002268
    CSI 6.39
    rCSI 34.77%
    PRS 43.9
  • CD14-positive, CD16-positive monocyte CL0002397
    CSI 6.2
    rCSI 8.12%
    PRS 27.02
  • choroid plexus epithelial cell CL0000706
    CSI 6.16
    rCSI 10.09%
    PRS 14.59
  • melanocyte CL0000148
    CSI 6.01
    rCSI 4.45%
    PRS 16.51
  • conjunctival epithelial cell CL1000432
    CSI 5.98
    rCSI 9.13%
    PRS 19.09
  • thymocyte CL0000893
    CSI 5.77
    rCSI 20.52%
    PRS 55.56
  • intermediate monocyte CL0002393
    CSI 5.76
    rCSI 8.69%
    PRS 19.15
  • mammary gland epithelial cell CL0002327
    CSI 5.73
    rCSI 20.1%
    PRS 32.98
  • hematopoietic precursor cell CL0008001
    CSI 5.54
    rCSI 5.7%
    PRS 30.75
  • myofibroblast cell CL0000186
    CSI 5.53
    rCSI 7.66%
    PRS 26.8
  • vascular associated smooth muscle cell CL0000359
    CSI 5.48
    rCSI 17.79%
    PRS 22.84
  • erythrocyte CL0000232
    CSI 5.35
    rCSI 12.13%
    PRS 25.72
  • retinal cone cell CL0000573
    CSI 5.34
    rCSI 8.6%
    PRS 14.55
  • effector CD8-positive, alpha-beta T cell CL0001050
    CSI 5.31
    rCSI 4.04%
    PRS 25.07
  • pulmonary capillary endothelial cell CL4028001
    CSI 5.31
    rCSI 10.13%
    PRS 30.01
  • effector CD4-positive, alpha-beta T cell CL0001044
    CSI 5.25
    rCSI 15.05%
    PRS 28.13
  • radial glial cell CL0000681
    CSI 5.2
    rCSI 7.23%
    PRS 19.41
  • ciliated cell CL0000064
    CSI 5.13
    rCSI 8.32%
    PRS 19.17
  • plasma cell CL0000786
    CSI 5.1
    rCSI 6.69%
    PRS 66.01
  • mast cell CL0000097
    CSI 5.09
    rCSI 10.98%
    PRS 65.41
  • lung secretory cell CL1000272
    CSI 5.08
    rCSI 12.58%
    PRS 17.52
  • CD16-positive, CD56-dim natural killer cell, human CL0000939
    CSI 5
    rCSI 3.33%
    PRS 45.7
  • intestinal tuft cell CL0019032
    CSI 4.96
    rCSI 7.58%
    PRS 21.73
  • erythroid lineage cell CL0000764
    CSI 4.88
    rCSI 31.38%
    PRS 41.46
  • enteroendocrine cell CL0000164
    CSI 4.86
    rCSI 6.64%
    PRS 21.02
  • glial cell CL0000125
    CSI 4.86
    rCSI 18.49%
    PRS 18.5
  • CD16-negative, CD56-bright natural killer cell, human CL0000938
    CSI 4.85
    rCSI 3.64%
    PRS 51.3
  • CD4-positive, alpha-beta thymocyte CL0000810
    CSI 4.77
    rCSI 3.82%
    PRS 33.86
  • blood vessel endothelial cell CL0000071
    CSI 4.77
    rCSI 9.9%
    PRS 18.91
  • retinal blood vessel endothelial cell CL0002585
    CSI 4.75
    rCSI 7.58%
    PRS 20.82
  • IgG plasma cell CL0000985
    CSI 4.73
    rCSI 5.67%
    PRS 32.79
  • unswitched memory B cell CL0000970
    CSI 4.7
    rCSI 3.96%
    PRS 30.09
  • basal cell CL0000646
    CSI 4.65
    rCSI 6.22%
    PRS 20.81
  • elicited macrophage CL0000861
    CSI 4.64
    rCSI 4.26%
    PRS 22.18
  • mononuclear phagocyte CL0000113
    CSI -3.9
    rCSI -8.6%
    PRS 21.4%
  • CD8-positive, alpha-beta cytotoxic T cell CL0000794
    CSI -1.5
    rCSI -1.7%
    PRS 33.1%
  • respiratory hillock cell CL4030023
    CSI -0.7
    rCSI -1.2%
    PRS 31.5%
  • kidney connecting tubule epithelial cell CL1000768
    CSI 0.1
    rCSI 0.3%
    PRS 14.4%
  • sst GABAergic cortical interneuron CL4023017
    CSI 0.1
    rCSI 0.2%
    PRS 11.7%
  • cytotoxic T cell CL0000910
    CSI 0.2
    rCSI 1.3%
    PRS 27.8%
  • epithelial cell of esophagus CL0002252
    CSI 0.3
    rCSI 2.5%
    PRS 55.8%
  • prostate gland microvascular endothelial cell CL2000059
    CSI 0.3
    rCSI 6.1%
    PRS 55.6%
  • paneth cell CL0000510
    CSI 0.3
    rCSI 0.4%
    PRS 29.6%
  • osteoblast CL0000062
    CSI 0.3
    rCSI 7.1%
    PRS 80.8%
  • follicular dendritic cell CL0000442
    CSI 0.3
    rCSI 4.7%
    PRS 69.6%
  • common lymphoid progenitor CL0000051
    CSI 0.3
    rCSI 0.4%
    PRS 36.0%
  • L6b glutamatergic cortical neuron CL4023038
    CSI 0.3
    rCSI 0.9%
    PRS 11.9%
  • Hofbauer cell CL3000001
    CSI 0.3
    rCSI 0.6%
    PRS 23.9%
  • lung microvascular endothelial cell CL2000016
    CSI 0.4
    rCSI 7.1%
    PRS 52.5%
  • respiratory basal cell CL0002633
    CSI 0.4
    rCSI 0.4%
    PRS 22.3%
  • respiratory goblet cell CL0002370
    CSI 0.4
    rCSI 4.6%
    PRS 36.0%
  • bronchiolar smooth muscle cell CL4033017
    CSI 0.4
    rCSI 6.4%
    PRS 52.2%
  • intestinal crypt stem cell of colon CL0009043
    CSI 0.4
    rCSI 3.3%
    PRS 34.5%
  • CD34-positive, CD56-positive, CD117-positive common innate lymphoid precursor, human CL0001074
    CSI 0.5
    rCSI 5.2%
    PRS 63.1%
  • peptic cell CL0000155
    CSI 0.5
    rCSI 5.0%
    PRS 49.2%
  • respiratory epithelial cell CL0002368
    CSI 0.5
    rCSI 3.3%
    PRS 55.6%
  • mature alpha-beta T cell CL0000791
    CSI 0.6
    rCSI 2.0%
    PRS 32.5%
  • lymphoid lineage restricted progenitor cell CL0000838
    CSI 0.6
    rCSI 2.3%
    PRS 31.2%
  • eosinophil CL0000771
    CSI 0.6
    rCSI 4.0%
    PRS 47.3%
  • colon macrophage CL0009038
    CSI 0.6
    rCSI 2.8%
    PRS 39.1%
  • CD8-positive, alpha-beta memory T cell, CD45RO-positive CL0001203
    CSI 0.6
    rCSI 0.8%
    PRS 23.6%
  • transit amplifying cell of small intestine CL0009012
    CSI 0.6
    rCSI 2.8%
    PRS 35.0%
  • parietal epithelial cell CL1000452
    CSI 0.7
    rCSI 1.9%
    PRS 15.8%
  • brush cell CL0002204
    CSI 0.7
    rCSI 1.4%
    PRS 45.8%
  • basophil CL0000767
    CSI 0.7
    rCSI 1.5%
    PRS 37.8%
  • IgM plasma cell CL0000986
    CSI 0.7
    rCSI 3.3%
    PRS 70.1%
  • vasa recta descending limb cell CL1001285
    CSI 0.7
    rCSI 5.9%
    PRS 62.9%
  • enteroendocrine cell of colon CL0009042
    CSI 0.8
    rCSI 3.6%
    PRS 46.9%
  • vasa recta ascending limb cell CL1001131
    CSI 0.8
    rCSI 3.5%
    PRS 57.2%
  • BEST4+ enteroycte CL4030026
    CSI 0.8
    rCSI 1.0%
    PRS 20.2%
  • neuroendocrine cell CL0000165
    CSI 0.8
    rCSI 3.1%
    PRS 37.4%
  • colon goblet cell CL0009039
    CSI 0.8
    rCSI 1.9%
    PRS 28.4%
  • antibody secreting cell CL0000946
    CSI 0.8
    rCSI 3.6%
    PRS 65.9%
  • chondrocyte CL0000138
    CSI 0.8
    rCSI 1.3%
    PRS 16.0%
  • enteric neuron CL0007011
    CSI 0.8
    rCSI 12.4%
    PRS 45.5%
  • basophil mast progenitor cell CL0002028
    CSI 0.9
    rCSI 4.6%
    PRS 61.6%
  • keratocyte CL0002363
    CSI 0.9
    rCSI 2.1%
    PRS 28.0%
  • class switched memory B cell CL0000972
    CSI 0.9
    rCSI 0.7%
    PRS 31.8%
  • retinal rod cell CL0000604
    CSI 0.9
    rCSI 1.6%
    PRS 18.7%
  • enteroendocrine cell of small intestine CL0009006
    CSI 0.9
    rCSI 2.1%
    PRS 28.8%
  • acinar cell of salivary gland CL0002623
    CSI 0.9
    rCSI 21.9%
    PRS 35.3%
  • vein endothelial cell of respiratory system CL4033008
    CSI 1.0
    rCSI 6.5%
    PRS 36.6%
  • metallothionein-positive alveolar macrophage CL4033042
    CSI 1.0
    rCSI 10.3%
    PRS 61.5%
  • basal cell of epithelium of trachea CL1000348
    CSI 1.0
    rCSI 6.8%
    PRS 51.8%
  • odontoblast CL0000060
    CSI 1.0
    rCSI 22.6%
    PRS 65.6%
  • intraepithelial lymphocyte CL0002496
    CSI 1.0
    rCSI 2.8%
    PRS 64.1%
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 1.1
    rCSI 2.6%
    PRS 11.0%
  • paneth cell of colon CL0009009
    CSI 1.1
    rCSI 10.5%
    PRS 48.9%
  • basal cell of prostate epithelium CL0002341
    CSI 1.1
    rCSI 3.1%
    PRS 40.4%
  • type L enteroendocrine cell CL0002279
    CSI 1.1
    rCSI 2.0%
    PRS 37.0%
  • rod bipolar cell CL0000751
    CSI 1.1
    rCSI 2.0%
    PRS 15.7%
  • small pre-B-II cell CL0000954
    CSI 1.1
    rCSI 1.1%
    PRS 38.4%
  • tracheal goblet cell CL1000329
    CSI 1.1
    rCSI 2.4%
    PRS 36.8%
  • CD4-positive, alpha-beta cytotoxic T cell CL0000934
    CSI 1.2
    rCSI 1.6%
    PRS 37.6%
  • alternatively activated macrophage CL0000890
    CSI 1.2
    rCSI 1.5%
    PRS 29.1%
  • kidney loop of Henle thin ascending limb epithelial cell CL1001107
    CSI 1.2
    rCSI 3.1%
    PRS 17.7%
  • glandular epithelial cell CL0000150
    CSI 1.2
    rCSI 3.3%
    PRS 36.7%
  • myeloid dendritic cell CL0000782
    CSI 1.3
    rCSI 1.8%
    PRS 28.5%
  • OFF-bipolar cell CL0000750
    CSI 1.3
    rCSI 1.7%
    PRS 29.8%
  • mucous neck cell CL0000651
    CSI 1.3
    rCSI 1.8%
    PRS 30.0%
  • dendritic cell, human CL0001056
    CSI 1.3
    rCSI 2.0%
    PRS 22.6%
  • endocrine cell CL0000163
    CSI 1.3
    rCSI 6.7%
    PRS 61.9%
  • hematopoietic multipotent progenitor cell CL0000837
    CSI 1.3
    rCSI 3.2%
    PRS 30.0%
  • ventricular cardiac muscle cell CL2000046
    CSI 1.4
    rCSI 4.6%
    PRS 63.1%
  • central memory CD8-positive, alpha-beta T cell CL0000907
    CSI 1.4
    rCSI 0.9%
    PRS 23.2%
  • smooth muscle cell CL0000192
    CSI 1.4
    rCSI 3.3%
    PRS 48.2%
  • mature B cell CL0000785
    CSI 1.4
    rCSI 1.2%
    PRS 23.8%
  • renal beta-intercalated cell CL0002201
    CSI 1.4
    rCSI 3.4%
    PRS 21.9%
  • Kupffer cell CL0000091
    CSI 1.5
    rCSI 3.3%
    PRS 18.5%
  • type EC enteroendocrine cell CL0000577
    CSI 1.5
    rCSI 5.2%
    PRS 30.8%
  • luminal epithelial cell of mammary gland CL0002326
    CSI 1.5
    rCSI 2.7%
    PRS 28.9%
  • T-helper 17 cell CL0000899
    CSI 1.5
    rCSI 1.2%
    PRS 33.6%
  • bronchial goblet cell CL1000312
    CSI 1.6
    rCSI 6.3%
    PRS 39.9%
  • blood vessel smooth muscle cell CL0019018
    CSI 1.6
    rCSI 13.0%
    PRS 19.2%
  • mesodermal cell CL0000222
    CSI 1.6
    rCSI 1.9%
    PRS 18.5%
  • tracheobronchial serous cell CL0019001
    CSI 1.6
    rCSI 7.0%
    PRS 34.9%
  • hepatocyte CL0000182
    CSI 1.6
    rCSI 2.9%
    PRS 17.6%
  • luminal cell of prostate epithelium CL0002340
    CSI 1.6
    rCSI 8.8%
    PRS 33.5%
  • muscle cell CL0000187
    CSI 1.7
    rCSI 3.4%
    PRS 41.0%
  • erythroblast CL0000765
    CSI 1.7
    rCSI 4.5%
    PRS 30.3%
  • pre-conventional dendritic cell CL0002010
    CSI 1.7
    rCSI 22.5%
    PRS 54.5%
  • CD8-positive, CD28-negative, alpha-beta regulatory T cell CL0000920
    CSI 1.7
    rCSI 3.5%
    PRS 31.6%
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 1.8
    rCSI 3.1%
    PRS 11.2%
  • placental villous trophoblast CL2000060
    CSI 1.8
    rCSI 2.7%
    PRS 17.8%
  • germinal center B cell CL0000844
    CSI 1.9
    rCSI 5.5%
    PRS 43.4%
  • foveolar cell of stomach CL0002179
    CSI 1.9
    rCSI 4.0%
    PRS 30.2%
  • extravillous trophoblast CL0008036
    CSI 1.9
    rCSI 2.4%
    PRS 16.6%
  • CD14-low, CD16-positive monocyte CL0002396
    CSI 1.9
    rCSI 1.5%
    PRS 17.5%
  • renal principal cell CL0005009
    CSI 1.9
    rCSI 5.0%
    PRS 24.5%
  • alveolar type 1 fibroblast cell CL4028004
    CSI 1.9
    rCSI 2.1%
    PRS 21.5%
  • podocyte CL0000653
    CSI 1.9
    rCSI 8.6%
    PRS 18.6%
  • myoepithelial cell CL0000185
    CSI 1.9
    rCSI 4.9%
    PRS 23.5%
  • megakaryocyte progenitor cell CL0000553
    CSI 2.0
    rCSI 35.6%
    PRS 52.4%
  • keratinocyte CL0000312
    CSI 2.0
    rCSI 1.7%
    PRS 22.6%

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.

Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [NDUFAF3](/details-gene/25915) is a protein-coding gene located on chromosome 3 that encodes the NADH:ubiquinone oxidoreductase complex assembly factor 3. This protein plays an essential role in the biogenesis of mitochondrial respiratory chain Complex I, the first and largest enzyme complex of the electron transport chain. Consistent with its fundamental role in aerobic respiration, [NDUFAF3](/details-gene/25915) shows significant expression in cells with high energy demands, such as [ciliated epithelial cell](/details-cell/CL0000067)s and various progenitor cells. Mutations in [NDUFAF3](/details-gene/25915) have been clinically linked to severe, often fatal, neonatal mitochondrial disorders, including Leigh syndrome, underscoring its critical importance in cellular metabolism and human health ([Link](https://doi.org/10.1016/j.ajhg.2009.04.020), [Link](https://doi.org/10.1016/j.ymgme.2016.12.005)). ## Cellular Roles and Expression Landscape The **Overall** expression profile of [NDUFAF3](/details-gene/25915) highlights its importance in cells requiring substantial energy production for motility, proliferation, and secretion. The gene demonstrates the highest significance in various types of ciliated cells, including [ciliated epithelial cell](/details-cell/CL0000067) (CSI: 75.08), [multi-ciliated epithelial cell](/details-cell/CL0005012) (CSI: 74.09), and [lung ciliated cell](/details-cell/CL1000271) (CSI: 30.06). This pattern strongly suggests that robust mitochondrial function, facilitated by [NDUFAF3](/details-gene/25915), is indispensable for supplying the high levels of ATP needed to power ciliary movement in tissues like the respiratory and reproductive tracts. Furthermore, [NDUFAF3](/details-gene/25915) is a significant marker in several progenitor cell populations, such as [megakaryocyte-erythroid progenitor cell](/details-cell/CL0000050) (CSI: 53.48), [fraction A pre-pro B cell](/details-cell/CL0002045) (CSI: 28.41), and [granulocyte monocyte progenitor cell](/details-cell/CL0000557) (CSI: 25.75). This indicates a crucial role for efficient energy metabolism during the highly active processes of cell division and differentiation in hematopoietic lineages. The gene is also prominent in metabolically active, terminally differentiated cells like [cardiac muscle cell](/details-cell/CL0000746) (CSI: 30.02) and secretory cells such as [pancreatic A cell](/details-cell/CL0000171) (CSI: 35.46) and [fallopian tube secretory epithelial cell](/details-cell/CL4030006) (CSI: 32.36). Conversely, the low significance of [NDUFAF3](/details-gene/25915) in cell types such as [CD8-positive, alpha-beta cytotoxic T cell](/details-cell/CL0000794) (CSI: -1.45) suggests that its role may be less central in certain mature immune effector cells, which can rely more on glycolytic pathways for rapid energy bursts. ## Pathways and Molecular Function Functional annotations confirm the central role of [NDUFAF3](/details-gene/25915) in mitochondrial energy metabolism. The gene product is localized to the [mitochondrial inner membrane](/details-go/GO:0005743) and is a key participant in [mitochondrial respiratory chain complex i assembly](/details-go/GO:0032981). Its involvement in [protein binding](/details-go/GO:0005515) is consistent with its function as an assembly factor that interacts with other subunits to construct the functional Complex I enzyme. This molecular function is a critical component of several major metabolic pathways cataloged by Reactome, including [Complex i biogenesis](/details-pathway/R-HSA-6799198), which is nested within the broader processes of [respiratory electron transport](/details-pathway/R-HSA-611105) and overall [metabolism](/details-pathway/R-HSA-1430728). The efficient assembly of Complex I is the rate-limiting first step for NADH oxidation and proton pumping, which establishes the electrochemical gradient necessary for ATP synthesis. ## Research Directions The expression data and known function of [NDUFAF3](/details-gene/25915) point to its indispensable role in cellular bioenergetics, with clear clinical implications. Future research can build upon this foundation to explore its cell-specific functions and therapeutic potential. **Testable Hypotheses:** 1. The exceptionally high significance of [NDUFAF3](/details-gene/25915) in ciliated cells suggests that its expression level is a key determinant of ciliary beat frequency and mucociliary clearance. Consequently, subtle hypomorphic variants in [NDUFAF3](/details-gene/25915) may contribute to the pathology of primary ciliary dyskinesia (PCD) or other ciliopathies. 2. Given its high expression in hematopoietic progenitors, [NDUFAF3](/details-gene/25915) may play a critical regulatory role during lineage commitment. Its downregulation could impair the differentiation of myeloid and erythroid lineages, which are particularly dependent on oxidative phosphorylation. **Proposed Experimental Approach:** To test the hypothesis regarding its role in ciliary function, one could use an in vitro model of the human airway epithelium. Primary human bronchial epithelial cells could be cultured at an air-liquid interface to induce differentiation into a ciliated epithelium. A CRISPR interference (CRISPRi) system could then be employed to achieve a tunable knockdown of [NDUFAF3](/details-gene/25915) expression. The functional consequences would be assessed by measuring ciliary beat frequency using high-speed video microscopy and quantifying ATP levels within the cells. A positive result would demonstrate a direct, dose-dependent relationship between [NDUFAF3](/details-gene/25915) expression and ciliary motor function. **Therapeutic Potential:** Because loss-of-function mutations in [NDUFAF3](/details-gene/25915) cause severe mitochondrial disease, the therapeutic strategy would be focused on **activation or functional restoration**, not inhibition. [NDUFAF3](/details-gene/25915) is a poor candidate for inhibitory drugs due to its essential role in healthy, high-energy tissues. For monogenic diseases like Leigh syndrome caused by [NDUFAF3](/details-gene/25915) mutations, gene therapy represents a plausible, albeit challenging, therapeutic avenue. This could involve the delivery of a functional copy of the gene to affected tissues, such as the central nervous system or muscle, using adeno-associated virus (AAV) vectors to restore Complex I assembly and mitochondrial function.

Genular Protein ID: 1787592808

Symbol: NDUF3_HUMAN

Name: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 3

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 19463981

Title: Mutations in NDUFAF3 (C3ORF60), encoding an NDUFAF4 (C6ORF66)-interacting complex I assembly protein, cause fatal neonatal mitochondrial disease.

PubMed ID: 19463981

DOI: 10.1016/j.ajhg.2009.04.020

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 25944712

Title: N-terminome analysis of the human mitochondrial proteome.

PubMed ID: 25944712

DOI: 10.1002/pmic.201400617

PubMed ID: 27986404

Title: Mutations in mitochondrial complex I assembly factor NDUFAF3 cause Leigh syndrome.

PubMed ID: 27986404

DOI: 10.1016/j.ymgme.2016.12.005

Sequence Information:

  • Length: 184
  • Mass: 20350
  • Checksum: FCFF7B0CB6ADBCD8
  • Sequence:
  • MATALALRSL YRARPSLRCP PVELPWAPRR GHRLSPADDE LYQRTRISLL QREAAQAMYI 
    DSYNSRGFMI NGNRVLGPCA LLPHSVVQWN VGSHQDITED SFSLFWLLEP RIEIVVVGTG 
    DRTERLQSQV LQAMRQRGIA VEVQDTPNAC ATFNFLCHEG RVTGAALIPP PGGTSLTSLG 
    QAAQ

Genular Protein ID: 2170835456

Symbol: A4FU71_HUMAN

Name: N/A

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 11181995

Title: The sequence of the human genome.

PubMed ID: 11181995

DOI: 10.1126/science.1058040

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 127
  • Mass: 13842
  • Checksum: 4D795EDA0408C242
  • Sequence:
  • MYIDSYNSRG FMINGNRVLG PCALLPHSVV QWNVGSHQDI TEDSFSLFWL LEPRIEIVVV 
    GTGDRTERLQ SQVLQAMRQR GIAVEVQDTP NACATFNFLC HEGRVTGAAL IPPPGGTSLT 
    SLGQAAQ

Genular Protein ID: 3700458268

Symbol: Q9Y3Z0_HUMAN

Name: N/A

UniProtKB Accession Codes:

Database IDs:

Sequence Information:

  • Length: 127
  • Mass: 13866
  • Checksum: 486D5EDA0408C242
  • Sequence:
  • MYIDSYNSRG FMINGNRVLG PCALLPHSVV QWNVGSHQDI TEDSFSLFWL LEPRIEIVVV 
    GTGDRTERLQ SQVLQAMRQR GIAVEVQDTP NACATFNFLC HEGRVTGAAL IPPPGGTSHT 
    SLGQAAQ